In vitro anti-Trichomonas gallinae effects of Ziziphus vulgaris L. and Camellia sinensis (L.) Kuntze extracts.

BACKGROUND: Trichomonas gallinae is a parasite that causes canker and severe loss and death, especially in young pigeons. Metronidazole (MTZ) is the recommended drug for treating avian trichomoniasis. Due to drug resistance, non-chemical alternatives, such as medicinal plant extracts, are also considered possible therapies for this disease. OBJECTIVES: This study compares the antitrichomonal effects of MTZ with extracts of Camellia sinensis and Ziziphus vulgaris on T. gallinae in vitro. METHODS: Samples of T. gallinae were taken from infected pigeons. Multi-well plates with different concentrations (5, 10, 25, 50 and 100 µg/mL) of plant extracts were used for the in vitro study. RESULTS: The minimum inhibitory concentration (MIC) of C. sinensis extract was 25 µg/mL over 24 h, compared to 50 µg/mL for MTZ. The MIC value of the Z. vulgaris extracts was 50 µg/mL. CONCLUSIONS: The results suggest that the extracts of Z. vulgaris and C. sinensis, as potential natural agents, could have anti-avian trichomoniasis properties. This study also shows that MTZ, C. sinensis and Z. vulgaris are equally effective in preventing the growth of T. gallinae trophozoites in the culture.

Examining the associations between perceived built environment and active travel, before and after the COVID-19 outbreak in Shiraz city, Iran.

This study aims to evaluate the effects of the COVID-19 pandemic and policies to control the outbreak such as quarantine, jobs closures, and traffic restrictions on active travel of Central Businesses District (CBD) residents in comparison with the non-CBD residents of Shiraz, Iran; and examine the relationship between perceived built environment factors and active travel in the pre- and post-outbreak. The results indicate that the most effective individuals factors on active travel are bicycle and car ownership, and built environment characteristics are walkability, bikeability, security, aesthetics, traffic calming, intersections safety, land uses diversity and density, destination accessibility, street pattern, and bike-sharing infrastructures. Also, the average walking and cycling time of the CBD residents before and after the outbreak is more than that of the non-CBD residents, which is due to the quality of built environment factors in the CBD. A built environment with mixed, diverse, dense and accessible land uses, as well as safe and secure cycling and walking routes have major effects on active travel in the crisis. Hence, it is suggested that policymakers take action to make the environment more people-friendly to maintain citizens' mobility in the critical situation, when many travel modes have lost their efficiency.

Monitoring of the Physical and Chemical Properties of a Gasoline Engine Oil during Its Usage.

Physicochemical properties of a mineral-based gasoline engine oil have been monitored at 0, 500, 1000, 2000, 3500, 6000, 8500, and 11500 kilometer of operation. Tracing has been performed by inductively coupled plasma and some other techniques. At each series of measurements, the concentrations of twenty four elements as well as physical properties such as: viscosity at 40 and 100°C; viscosity index; flash point; pour point; specific gravity; color; total acid and base numbers; water content have been determined. The results are indicative of the decreasing trend in concentration of additive elements and increasing in concentration for wear elements. Different trends have been observed for various physical properties. The possible reasons for variations in physical and chemical properties have been discussed.