Virtual Hall sensor triggered multi-MHz endoscopic OCT imaging for stable real-time visualization.

Circumferential scanning in endoscopic imaging is crucial across various disciplines, and optical coherence tomography (OCT) is often the preferred choice due to its high-speed, high-resolution, and micron-scale imaging capabilities. Moreover, real-time and high-speed 3D endoscopy is a pivotal technology for medical screening and precise surgical guidance, among other applications. However, challenges such as image jitter and non-uniform rotational distortion (NURD) are persistent obstacles that hinder real-time visualization during high-speed OCT procedures. To address this issue, we developed an innovative, low-cost endoscope that employs a brushless DC motor for scanning, and a sensorless technique for triggering and synchronizing OCT imaging with the scanning motor. This sensorless approach uses the motor's electrical feedback (back electromotive force, BEMF) as a virtual Hall sensor to initiate OCT image acquisition and synchronize it with a Fourier Domain Mode-Locked (FDML)-based Megahertz OCT system. Notably, the implementation of BEMF-triggered OCT has led to a substantial reduction in image jitter and NURD (<4 mrad), thereby opening up a new window for real-time visualization capabilities. This approach suggests potential benefits across various applications, aiming to provide a more accurate, deployable, and cost-effective solution. Subsequent studies can explore the adaptability of this system to specific clinical scenarios and its performance under practical endoscopic conditions.

High-resolution rectoscopy using MHz optical coherence tomography: a step towards real time 3D endoscopy.

Colonoscopy and endoscopic ultrasound play pivotal roles in the assessment of rectal diseases, especially rectal cancer and inflammatory bowel diseases. Optical coherence tomography (OCT) offers a superior depth resolution, which is a critical factor for individualizing the therapeutic concept and evaluating the therapy response. We developed two distinct rectoscope prototypes, which were integrated into a 1300 nm MHz-OCT system constructed at our facility. The rapid rotation of the distal scanning probe at 40,000 revolutions per minute facilitates a 667 Hz OCT frame rate, enabling real-time endoscopic imaging of large areas. The performance of these OCT-rectoscopes was assessed in an ex vivo porcine colon and a post mortem human in-situ colon. The OCT-rectoscope consistently distinguished various layers of the intestinal wall, identified gut-associated lymphatic tissue, and visualized a rectal polyp during the imaging procedure with 3D-reconstruction in real time. Subsequent histological examination confirmed these findings. The body donor was preserved using an ethanol-glycerol-lysoformin-based technique for true-to-life tissue consistency. We could demonstrate that the novel MHZ-OCT-rectoscope effectively discriminates rectal wall layers and crucial tissue characteristics in a post mortem human colon in-situ. This real-time-3D-OCT holds promise as a valuable future diagnostic tool for assessing disease state and therapy response on-site in rectal diseases.

Experimental and theoretical study of the formation process of photopolymer based self-written waveguides.

The realization of optical interconnects between multimode (MM) optical fibers and waveguides based on a self-writing process in photopolymer media represents an efficient approach for fast and easy-to-implement connection of light-guiding elements. When light propagates through photopolymer media, it modulates the material properties of the media and confines the spreading of the light beam to create a waveguide along the beam propagation direction. This self-writing process can be realized with a single photopolymer medium and is also suited to connect optical fibers or waveguides with active elements such as light sources and detectors. Numerical simulations of the underlying light-induced polymerization process is carried out by using a diffusion based material model which takes account both monomer diffusion and its conversion to polymer chains in regions exposed to light fields. In this work experimental results obtained from a one-polymer approach are validated with theoretical predictions from the diffusion model. The study involved the demonstration of temporal dynamics and transmittance from self-written waveguide (SWW) couplers during the self-writing process. The measured attenuation coefficient from experiment αexperiment = (8.43 ± 0.3) × 10-5 dB/µm showed good agreement with the theoretically predicted attenuation coefficient αsimulation = 7.93 × 10-5 dB/µm, thus demonstrating a successful application of the diffusion model to epoxy based acrylate SWWs. For comparison, attenuation measurements between optical fibers with SWWs as interconnects and one without SWW, i.e. with an air gap in between, were performed. The obtained results reveal that the theoretical approach correctly describes the waveguide formation process so that in the next step the studies can be extended towards including further relevant parameters such as temperature.

Double Interferometer Design for Independent Wavefront Manipulation in Spectral Domain Optical Coherence Tomography.

Spectral domain optical coherence tomography (SD-OCT) is a highly versatile method which allows for three dimensional optical imaging in scattering media. A number of recent publications demonstrated the technique to benefit from structured illumination and beam shaping approaches, e.g. to enhance the signal-to-noise ratio or the penetration depth with samples such as biological tissue. We present a compact and easy to implement design for independent wavefront manipulation and beam shaping at the reference and sample arm of the interferometric OCT device. The design requires a single spatial light modulator and can be integrated to existing free space SD-OCT systems by modifying the source arm only. We provide analytical and numerical discussion of the presented design as well as experimental data confirming the theoretical analysis. The system is highly versatile and lends itself for applications where independent phase or wavefront control is required. We demonstrate the system to be used for wavefront sensorless adaptive optics as well as for iterative optical wavefront shaping for OCT signal enhancement in strongly scattering media.

Iterative wavefront correction for complex spectral domain optical coherence tomography.

We propose a compact setup for wavefront manipulation in spectral domain optical coherence tomography (OCT). The system can easily be implemented into existing free-space OCT setups through modification of the source path only. We demonstrate complex-valued OCT signal acquisition based on phase shifting combined with iterative optical wavefront shaping, which locally enhances the OCT signal acquired from within a scattering sample. The system lends itself to future imaging studies in strongly scattering media such as biological tissue.

Polymer-based diffractive optical elements for rear end automotive applications: design and fabrication process.

Advances in illumination technology in the automotive industry are heading toward the use of coherent sources for adaptable and high-resolution head lamps as well as interior and rear-end lights. We present a cost-effective method to fabricate and design laser-based lighting systems for the rear end in automobiles and other vehicles. The design relies on using binary gratings to generate a desired intensity distribution. For cost-effective fabrication, an optical maskless UV lithography system that employs a spatial light modulator (SLM) for projection of the desired structure onto photoresist is introduced. To replicate the structures onto polymers like poly(methyl methacrylate) (PMMA), we use polydimethylsiloxane soft stamps and a hot embossing system. The experimental results show that the proposed design and fabrication process is promising for high-resolution rear-end lights that might be employed to project symbols or information to guide road users in future.

Trimodal system for in vivo skin cancer screening with combined optical coherence tomography-Raman and colocalized optoacoustic measurements.

A new multimodal system for rapid, noninvasive in vivo skin cancer screening is presented, combining optical coherence tomography (OCT) and optoacoustic (OA) modalities to provide precise tumor depth determination with a Raman spectroscopic modality capable of detecting the lesion type and, thus, providing diagnostic capability. Both OA and Raman setups use wide field skin illumination to ensure the compliance with maximum permissible exposure (MPE) requirements. The Raman signal is collected via the OCT scanning lens to maximize the signal-to-noise ratio of the measured signal while keeping radiation levels below MPE limits. OCT is used to optically determine the tumor thickness and for volumetric imaging whereas OA utilizes acoustic signals generated by optical absorption contrast for thickness determination at potentially higher penetration depths compared to OCT. Preliminary results of first clinical trials using our setup are presented. The measured lesion depth is in good agreement with histology results, while Raman measurements show distinctive differences between normal skin and melanocytic lesions, and, moreover, between different skin areas. In future, we will validate the setup presented for reliable detection of pathophysiological parameters, morphology and thickness of suspicious skin lesions.

Confocal signal evaluation algorithms for surface metrology: uncertainty and numerical efficiency.

Confocal microscopy is one of the dominating measurement techniques in surface metrology, with an enhanced lateral resolution compared to alternative optical methods. However, the axial resolution in confocal microscopy is strongly dependent on the accuracy of signal evaluation algorithms, which are limited by random noise. Here, we discuss the influence of various noise sources on confocal intensity signal evaluating algorithms, including center-of-mass, parabolic least-square fit, and cross-correlation-based methods. We derive results in closed form for the uncertainty in height evaluation on surface microstructures, also accounting for the number of axially measured intensity values and a threshold that is commonly applied before signal evaluation. The validity of our results is verified by numerical Monte Carlo simulations. In addition, we implemented all three algorithms and analyzed their numerical efficiency. Our results can serve as guidance for a suitable choice of measurement parameters in confocal surface topography measurement, and thus lead to a shorter measurement time in practical applications.

Comparative study of presurgical skin infiltration depth measurements of melanocytic lesions with OCT and high frequency ultrasound.

A reliable, fast, and non-invasive determination of melanoma thickness in vivo is highly desirable for clinical dermatology as it may facilitate the identification of surgical melanoma margins, determine if a sentinel node biopsy should be performed or not, and reduce the number of surgical interventions for patients. In this work, optical coherence tomography (OCT) and high frequency ultrasound (HFUS) are evaluated for quantitative in vivo preoperative assessment of the skin infiltration depth of melanocytic tissue. Both methods allow non-invasive imaging of skin at similar axial resolution. Comparison with the Breslow lesion thickness obtained from histopathology revealed that OCT is slightly more precise in terms of thickness determination while HFUS has better contrast. The latter does not require image post-processing, as necessary for the OCT images. The findings of our pilot study suggest that non-invasive OCT and HFUS are able to determine the infiltration depth of lesions like melanocytic nevi or melanomas preoperatively and in vivo with a precision comparable to invasive histopathology measurements on skin biopsies. In future, to further strengthen our findings a statistically significant study comprising a larger amount of data is required which will be conducted in an extended clinical study in the next step. Comparison of optical coherence tomography and high frequency ultrasound B-Scans and a H&E stained histology of a melanocytic nevus.

Autofocusing system for spatial light modulator-based maskless lithography.

To produce diffractive or holographic structures in a photolithographic process, an optical projection system enabling structure resolution in the submicrometer range is highly desirable. To ensure that the optical focus of such a system lies on the substrate surface during the whole lithographic fabrication process, an autofocus system able to focus on a depth of field of a few hundred nanometers is usually required. In this work, we developed an autofocus system for spatial light modulator (SLM)-based maskless photolithographic applications. The system is capable of high-precision focusing without affecting the photoresist performance. It is based on contrast measurement combined with focus-pattern illumination to ensure high contrast at the substrate surface. In addition, we evaluated various autofocus algorithms with respect to time efficiency and accuracy to determine suitable focus-pattern and focus-algorithm combinations.

Refractive-index measurement and inverse correction using optical coherence tomography.

We describe a novel technique for determination of the refractive index of hard biological tissue as well as nonopaque technical samples based on optical coherence tomography (OCT). Our method relies on an inverse refractive-index correction (I-RIC), which matches a measured feature geometry distorted due to refractive-index boundaries to its real geometry. For known feature geometry, the refractive index can be determined with high precision from the best match between the distorted and corrected images. We provide experimental data for refractive-index measurements on a polymethylmethacrylate (PMMA) and on an ex vivo porcine cranial-bone, which are compared to reference measurements and previously published data. Our method is potentially capable of in vivo measurements on rigid biological tissue such as bone as, for example, is required to improve guidance in robot-aided surgical interventions and also for retrieving complex refractive-index profiles of compound materials.

Systematic errors on curved microstructures caused by aberrations in confocal surface metrology.

Optical aberrations of microscope lenses are known as a source of systematic errors in confocal surface metrology, which has become one of the most popular methods to measure the surface topography of microstructures. We demonstrate that these errors are not constant over the entire field of view but also depend on the local slope angle of the microstructure and lead to significant deviations between the measured and the actual surface. It is shown by means of a full vectorial high NA numerical model that a change in the slope angle alters the shape of the intensity depth response of the microscope and leads to a shift of the intensity peak of up to several hundred nanometers. Comparative experimental data are presented which support the theoretical results. Our studies allow for correction of optical aberrations and, thus, increase the accuracy in profilometric measurements.

Flexible, fast, and low-cost production process for polymer based diffractive optics.

The generation of diffractive optical elements often requires time and cost consuming production techniques such as photolithography. Especially in research and development, small series of diffractive microstructures are needed and flexible and cost effective fabrication techniques are desirable to enable the fabrication of versatile optical elements on a short time scale. In this work, we introduce a novel process chain for fabrication of diffractive optical elements in various polymers. It is based on a maskless lithography process step, where a computer generated image of the optical element is projected via a digital mirror device and a microscope setup onto a silicon wafer coated with photosensitive resist. In addition, a stitching process allows us to microstructure a large area on the wafer. After development, a soft stamp of the microstructure is made from Polydimethylsiloxane, which is used as a mold for the subsequent hot embossing process, where the final diffractive optical element is replicated into thermoplastic polymer. Experimental results are presented, which demonstrate the applicability of the process.

Cladded self-written multimode step-index waveguides using a one-polymer approach.

Low-loss optical-coupling structures are highly relevant for applications in fields as diverse as information and communication technologies, integrated circuits, or flexible and highly-functional polymer sensor networks. For this suitable and reliable production methods are crucial. Self-written waveguides are an interesting solution. In this work, we present a simple and efficient one-polymer approach for self-written optical connections between light-guiding structures such as single-mode and multi-mode optical fibers or waveguides that relies on self focusing of the light inside a photopolymerizing mixture. The optical connections are produced in a two-step process by writing into monomer resin using cw laser light in the blue wavelength range and subsequent UV curing. Since only one photopolymerizing resin is required, we reduced the fabrication complexity compared to previous approaches to obtain a waveguide embedded in a rigid cladding material. We discuss the production method, the results obtained as function of relevant process parameters such as writing speed or curing time, and evaluate optical properties and coupling efficiencies.