Cunninghamella echinulata DSM1905 biofilm-based L-asparaginase production in pneumatically-driven bioreactors.

We evaluated by comparing the performance of three pneumatically-driven bioreactors in the production of L-asparaginase (L-ASNase), an enzyme used to treat leukaemia and lymphoma. A two-step screening process was conducted to detect Cunninghamella spp. strains producing L-ASNase. Cunninghamella echinulata DSM1905 produced the highest levels of L-ASNase during screening assays. Subsequently, fermentations were performed in bubble column (BCR), airlift (ALR), and hybrid fixed-bed airlift (FB-ALR) bioreactors to determine the best upstream bioprocess. Mycelial biomass production was higher in BCR than in ALR and FB-ALR (p ≤ 0.0322). The activity of L-ASNase produced in FB-ALR, in which the fungus grew as a consistent biofilm, was significantly higher (p ≤ 0.022) than that from ALR, which was higher than that of BCR (p = 0.036). The specific activity of ALR and FB-ALR presented no differences (p = 0.073), but it was higher than that of BCR (p ≤ 0.032). In conclusion, C. echinulata DSM1905, grown under the biofilm phenotype, produced the highest levels of L-ASNase, and FB-ALR was the best upstream system for enzyme production.

Central Carbon Metabolism in Candida albicans Biofilms Is Altered by Dimethyl Sulfoxide.

The effect of dimethyl sulfoxide (DMSO) on fungal metabolism has not been well studied. This study aimed to evaluate, by metabolomics, the impact of DMSO on the central carbon metabolism of Candida albicans. Biofilms of C. albicans SC5314 were grown on paper discs, using minimum mineral (MM) medium, in a dynamic continuous flow system. The two experimental conditions were control and 0.03% DMSO (v/v). After 72 h of incubation (37 °C), the biofilms were collected and the metabolites were extracted. The extracted metabolites were subjected to gas chromatography-mass spectrometry (GC/MS). The experiment was conducted using five replicates on three independent occasions. The GC/MS analysis identified 88 compounds. Among the 88 compounds, the levels of 27 compounds were markedly different between the two groups. The DMSO group exhibited enhanced levels of putrescine and glutathione and decreased levels of methionine and lysine. Additionally, the DMSO group exhibited alterations in 13 metabolic pathways involved in primary and secondary cellular metabolism. Among the 13 altered pathways, seven were downregulated and six were upregulated in the DMSO group. These results indicated a differential intracellular metabolic profile between the untreated and DMSO-treated biofilms. Hence, DMSO was demonstrated to affect the metabolic pathways of C. albicans. These results suggest that DMSO may influence the results of laboratory tests when it is used as a solvent. Hence, the use of DMSO as a solvent must be carefully considered in drug research, as the effect of the researched drugs may not be reliably translated into clinical practice.

Tratamento multidisciplinar de paciente adulto padrão face curta ­ relato de caso / Interdisciplinary treatment of short-face adult patient ­ case report

Resumo Introdução: As más-oclusões do Padrão Face Curta são responsáveis por prejuízos estéticos e funcionais, entretanto o diagnóstico tardio impede o tratamento ortopédico preventivo e a cirurgia ortognática pode ser uma opção para os pacientes desfuncionados. Objetivo: Descrever por meio de um relato de caso o tratamento ortodôntico descompensatório cirúrgico associado à reabilitação estética de um paciente Padrão Face Curta. Descrição: Paciente Padrão Face Curta, sexo masculino, 26 anos, leucoderma, relação de Classe II total de molar e canino, sobremordida e sobressalência aumentada, protrusão dentária superior, relação de lábio comprimido, terço inferior da face diminuído. O tratamento proposto foi o tratamento ortodôntico descompensatório cirúrgico associado à reabilitação estética, considerando a gravidade dos sinais apresentados e a queixa do paciente. No final do tratamento, houve o resgate do equilíbrio estético e oclusal, com a solução total das queixas do paciente. Conclusão: Neste relato de caso, pôde se verificar a necessidade da participação interdisciplinar para obter um melhor resultado em um paciente Padrão Face Curta. O diagnóstico e planejamento realizado pelo ortodontista e pelo cirurgião bucal foram essenciais para alcançar um bom acabamento estético e funcional. (AU)

Abstract Introduction: Short-face Pattern malocclusions are responsible for aesthetic and functional impairments; however, late diagnosis excludes preventive orthopedic treatment, and orthognathic surgery can be an option for dysfunctional patients. Objective: To describe through a case report the surgical decompensatory orthodontic treatment associated with aesthetic rehabilitation of a Short-faced Pattern patient. Description: Short-face Pattern patient, male, 26 years old, leucoderma, total Class II molar and canine relationship, increased overbite and overjet, maxillary teeth protrusion, compressed lip relationship, decreased lower third of the face. The proposed treatment was a surgical decompensatory orthodontic treatment associated with aesthetic rehabilitation, considering the severity of the signs presented and the patient's complaint. At the end of the treatment there was aesthetic and occlusal balance rescue, with total solution of the patient's complaints. Conclusion: In this case report, we can verify the need for interdisciplinary participation to obtain a better result in a Short-faced Pattern patient. The diagnosis and planning performed by the orthodontist and the oral surgeon were essential to reach a good aesthetic and functional finishing.(AU)

Bacteriophages as tools for biofilm biocontrol in different fields.

Microbial biofilms are difficult to control due to the limited accessibility that antimicrobial drugs and chemicals have to the entrapped inner cells. The extracellular matrix, binds water, contributes to altered cell physiology within biofilms and act as a barrier for most antiproliferative molecules. Thus, new strategies need to be developed to overcome biofilm vitality. In this review, based on 223 documents, the advantages, recommendations, and limitations of using bacteriophages as 'biofilm predators' are presented. The plausibility of using phages (bacteriophages and mycoviruses) to control biofilms grown in different environments is also discussed. The topics covered here include recent historical experiences in biofilm control/eradication using phages in medicine, dentistry, veterinary, and food industries, the pros and cons of their use, and the development of microbial resistance/immunity to such viruses.