Correlation Analysis between Intrarenal Small Artery Intimal Thickening and Clinicopathological Features and Prognosis in Primary Membranous Nephropathy Patients.

BACKGROUND: Primary membranous nephropathy (PMN) is the most common pathological type of nephrotic syndrome in adults. Intrarenal small artery intimal thickening can be observed in most renal biopsies. The purpose of this study was to investigate the association between intrarenal small artery intimal thickening and clinicopathological features and prognosis in PMN patients. METHODS: Data were continuously collected from patients who were diagnosed with PMN in Shenzhen Second People's Hospital (The First Affiliated Hospital of Shenzhen University) from 2008 to 2021 for a retrospective cohort study. Regression analysis and survival analysis were used to analyze the relationship between intrarenal small artery intimal thickening and renal prognosis in PMN patients. RESULTS: 300 PMN patients were enrolled in this study, including 165 patients (55%) with intrarenal small artery intimal thickening. Patients with intimal thickening were older, with higher BMI, systolic blood pressure and diastolic blood pressure, serum uric acid, a higher proportion of hypertension, acute kidney injury, nephrotic syndrome, more urine protein, and lower eGFR. Multivariate Cox regression analysis showed that after adjusting for age, gender, hypertension, BMI, urine protein, eGFR, and the use of ACEI/ARB and hormone immunosuppressants, intimal thickening was a risk factor for renal prognosis in PMN patients (HR = 3.68, 95% CI 1.36-9.96, p < 0.05). Kaplan-Meier survival curve analysis showed that the incidence of reaching the renal composite outcome was higher in the intimal thickening group (p < 0.05). CONCLUSION: The prognosis of PMN patients with intrarenal small artery intimal thickening is worse, so early intervention is very important for these patients.

In silico identification of natural compounds against SARS-CoV-2 main protease from Chinese herbal medicines.

Aims: To determine natural compounds with inhibitory effects toward SARS-CoV-2 Mpro from Chinese herbal medicines. Materials & methods: ∼1200 natural compounds from 19 Chinese herbal medicines were collected. Computational methods including molecular docking, drug-likeness assessment, molecular dynamics simulation and molecular mechanics Poisson-Boltzmann surface area analysis were combined to obtain potent inhibitors against SARS-CoV-2 Mpro. Results: Top 20 compounds mainly originated from Ranunculus ternatus and Picrasma quassioides exhibited low binding free energies which below -9.0 kcal/mol. Compounds Japonicone G and Picrasidine T were obtained with favorable drug-likeness. Moreover, the complex of Japonicone G and Mpro had prominent stability. Conclusion: Natural compound Japonicone G is highly promising as a potent inhibitor against SARS-CoV-2 for further study.

Discovery of 3CLpro inhibitor of SARS-CoV-2 main protease.

Coronavirus main protease (3CLpro), a special cysteine protease in coronavirus family, is highly desirable in the life cycle of coronavirus. Here, molecular docking, ADMET pharmacokinetic profiles and molecular dynamics (MD) simulation were performed to develop specific 3CLpro inhibitor. The results showed that the 137 compounds originated from Chinese herbal have good binding affinity to 3CLpro. Among these, Cleomiscosin C, (+)-Norchelidonine, Protopine, Turkiyenine, Isochelidonine and Mallotucin A possessed prominent drug-likeness properties. Cleomiscosin C and Turkiyenine exhibited excellent pharmacokinetic profiles. Furthermore, the complex of Cleomiscosin C with SARS-CoV-2 main protease presented high stability. The findings in this work indicated that Cleomiscosin C is highly promising as a potential 3CLpro inhibitor, thus facilitating the development of effective drugs for COVID-19.

In this work, computer aided drug design technology was used to study the main protease 3CLpro of novel coronavirus, and functional small molecules with inhibitory effects on novel coronavirus were screened from the compound library of natural products. The results showed that Cleomiscosin C is highly promising as a potential 3CLpro inhibitor with prominent binding affinity, pharmacokinetic profiles and stability.

A case report of systemic lupus erythematosus combined with central serous chorioretinopathy treated with glucocorticoids.

Glucocorticoids are generally contraindicated for use in central serous chorioretinopathy (CSC) because their use is considered to be an independent risk factor for CSC. There are rare reports regarding the treatment of systemic lupus erythematosus (SLE) combined with CSC. This current case report describes a rare case of a 24-year-old female patient with severely active SLE combined with CSC, whose vision was significantly restored after she was administered 120 mg methylprednisolone intravenously once a day for 3 days. This case report presents the clinical characteristics for the first time in terms of distinguishing between typical CSC and lupus chorioretinopathy. It also provides a review of the relevant literature. In patients with clinically severe active lupus nephritis combined with bilateral lupus chorioretinopathy, timely systemic application of appropriate doses of glucocorticoids is the preferred method to control the primary disease and serious ocular complications.

Highly-efficient selective recognition and rapid enrichment of chrysin by magnetic surface molecularly imprinted polymer.

As an important natural nutrient-like substance, chrysin is highly desirable in the field of health food. Here, to achieve the specific and efficient adsorption of chrysin, a magnetic surface molecularly imprinted polymer (chrysin/SMIPs) was fabricated by free radical polymerization of methacrylic acid onto the surface of functionalized magnetic Fe3O4 nanoparticles. The obtained chrysin/SMIPs showed favorable binding ability towards chrysin with 35.27 mg·g-1 maximum adsorption capacity in 20 min, prominent reusability with 9 cycles of adsorption-desorption and superior specific recognition with imprinting factor (IF) of 2.87 and selectivity coefficient (K) of 8.74, 5.64 and 8.64 corresponding to the interferents of genistein, daidzein and quercetin, respectively. Moreover, the chrysin/SMIPs showed high recoveries (91.13 % to 95.12 %) and precisions (RSDs from 2.51 % to 1.32 %) in the extraction of chrysin from real propolis samples. Therefore, chrysin/SMIPs is highly promising for the selective enrichment recovery of chrysin in health food.

Preparation of environment-friendly solid epoxy resin with high-toughness via one-step banburying.

Solid epoxy resin is highly desired in adhesives, electronic materials and coatings due to the attractive characteristics of solvent-free, highly efficient utilization and convenient storage and transportation. However, the challenges remain in fabricating high-toughness solid epoxy resin through a facile and efficient way. Here, a high-performance environment-friendly solid epoxy resin was fabricated by employing maleic anhydride grafted ethylene-vinyl acetate copolymer (EVA-g-MAH) as the flexibilizer via one-step banburying method. The results showed that the modified epoxy resin maintained a high glass transition temperature (T g) and thermal stability, while its impact strength, tensile toughness and flexural toughness were significantly increased compared with the neat epoxy resin. The impact strength, tensile toughness and flexural toughness of R-EM10 are improved 138%, 195% and 149%, respectively. The EVA-g-MAH was introduced in the epoxy matrix as a separate phase to increase toughness via transfer stress and dissipated energy. The attractive properties of this facile fabrication process and the high-toughness, as well as the environment-friendly performance make this solid epoxy highly promising for large-scale industrial application.

Myriophyllum spicatum Leaves: Aerophily for Gas Collection and Transportation in Water.

The unique living environment of aquatic plants makes them produce many fantastic properties different from land ones. For instance, the leaves of Myriophyllum spicatum show excellent hydrophobicity and aerophily characteristics. In this paper, the abundant morphological structure, composition, and aerophily properties of Myriophyllum spicatum leaves are revealed. The contact angle of the leaf surface can reach 122° in air, exhibiting wonderful gas collection ability under water. The results showed that the aerophily of the leaves is attributed to the multistage micro-nanostructure and waxy layer on the surface. The gas transportation toward the tips of leaves is based on the void gradient formed by the nanoscale morphology at different growth stages and the buoyancy as well. These features provide bionic experience for gas collection, bubble transportation, and liquid resistance reduction in water environments.

Magnetic Surface Molecularly Imprinted Polymer for Selective Adsorption of 4-Hydroxycoumarin.

4-hydroxyl coumarin (HC), an important intermediate during the synthesis procedure of rodenticide and anti-cardiovascular drug, shows highly medicinal value and economic value. To achieve the efficient adsorption of HC from natural biological samples, a novel magnetic surface molecularly imprinted polymer (HC/SMIPs) was constructed by employing methacrylic acid (MAA) as functional monomer, organic silane modified magnetic particles as matrix carrier and HC as template molecule. Due to the numerous specific imprinted cavities on the HC/SMIPs, the maximum adsorption capacity of HC/SMIPs for 4-hydroxycoumarin could reach to 22.78 mg g-1 within 20 min. In addition, HC/SMIPs exhibited highly selective adsorption for 4-hydroxycoumarin compared with other active drug molecules (osthole and rutin) and showed excellent regeneration performance. After 8 cycles of adsorption-desorption tests, the adsorption capacity of HC/SMIPs just slightly decreased by 6.64%. The efficient selective removal and easy recycle of 4-hydroxycoumarin from biological samples by HC/SMIPs made a highly promising to advance the application of imprinting polymers in complex practical environments.

Immunoglobulin heavy chain gene rearrangement in heavy chain deposition disease suggests it is a plasma cell disease: a case report.

Heavy chain deposition disease (HCDD) is characterized by the deposition of truncated monoclonal immunoglobulin heavy chains along glomerular basement membranes. Truncated heavy chains are thought to be associated with plasma cell disease (PCD), but previous bone marrow cytology tests showed that only 30% of HCDD cases are related to PCDs. We report the first known use of immunoglobulin heavy chain (IGH) gene rearrangement to diagnose a patient with γ3-HCDD, although bone marrow morphology test identified no abnormalities. Our findings provide strong evidence for a correlation between PCDs and HCDD, which could help understand the genetic background underlying abnormal heavy chains and assess disease prognosis. Further, concordant with previous findings, bortezomib-based chemotherapy had a good therapeutic effect in our patient. We summarize the experience of diagnosing and treating a case of HCDD, and combine this with a literature review to further explore the correlation between PCDs and HCDD, which has important clinical value.

Highly Efficient Self-Repairing Slippery Liquid-Infused Surface with Promising Anti-Icing and Anti-Fouling Performance.

Smart slippery liquid-infused porous surfaces (SLIPSs) have aroused remarkable attention owing to tremendous application foreground in biomedical instruments and industry. However, challenges still remain in fabricating durable SLIPSs. In this work, a fast and highly efficient self-repairing slippery surface (SPU-60M) was fabricated based on a polyurethane membrane and silicone oil. By introducing a great quantity of reversible disulfide bonds into the polymer backbone and hydrogen bonds in the polymer interchain, this SLIPS material could be quickly repaired in 15 min with 97.8% healing efficiency. Moreover, the self-healing efficiency could be maintained at 42.75% after the 10th cutting-healing cycle. Notably, SPU-60M showed excellent self-repairing ability not only in an ambient environment but also in an underwater environment and at ultralow temperatures. Besides, the icing delay time (DT) of SPU-60M could be prolonged to 1182 s at -15 °C, and the ice adhesion strength was only 10.33 kPa at -30 °C. In addition, SPU-60M had excellent anti-fouling performance with BSA adsorption of 2.41 µg/cm2 and Escherichia coli CFU counts of 41 × 104. These findings provide a facile way to design highly efficient self-repairing SLIPSs with multifunctionality.