Trace metals and nutrient analysis of marine fish species from the Gwadar coast.

Trace metals are naturally occurring metals found in very small concentrations in the environment. In the context of fish flesh, metals such as copper, calcium, potassium, sodium, zinc, iron, and manganese are absorbed by fish and play vital roles in various physiological functions. However, if these metals exceed the recommended limits set by WHO/FAO, they are termed 'toxic metals' due to their harmful impacts on both the fish and its consumers. Therefore, the present study aims to analyze the levels of protein, lipids, and certain metals-Aluminum (Al), Sodium (Na), Zinc (Zn), Titanium (Ti), Iron (Fe), Copper (Cu), Potassium (K), and Calcium (Ca) in three commercially important marine fishes i.e. Rastrelliger kanagurta, Sardinella abella, and Otolithes ruber. The study also aims to assess their potential impact on human health. The macro-Kjeldhal method and Soxhlet apparatus were used to estimate protein and lipid contents, while atomic absorption spectroscopy (AAS) was used to estimate trace metals found in fishes. The study found that these fish species are valuable sources of protein, lipids, and certain essential minerals. The protein content (CP) in these three species ranged from 63.35 to 86.57%, while lipid content was from 21.05 to 23.86%. The overall results of the trace metal concentrations analyzed in the present study revealed that Aluminum (Al), Sodium (Na), Zinc (Zn), Titanium (Ti), Copper (Cu), Potassium (K), and Calcium (Ca) were found in low concentration or traces and also within suitable ranges as set by WHO/FAO. However, Iron (Fe) was absent in all three species. Moreover, both copper and potassium were found in all three species, while Zinc was present in Rastrelliger kanagurta and Sardinella abella, calcium in Sardinella abella, and sodium in Otolithes ruber only. Titanium was recorded for the first time in S. abella. However, the total health risk assessment associated with these fish food consumption was measured by THQ and TTHQ and found to be less than 1, which shows no potential risk related to trace metals found in these fishes on human health upon their consumption. In conclusion, these commercially important marine fish species were found valuable sources of protein, lipids, and essential trace minerals that are necessary for human health. Thus, the current study provides useful information for the local population to make informed decisions about their daily diets and highlights the importance of sustainable fishing practices to maintain these valuable marine resources by periodical monitoring of their ecosystem.

Elemental composition of three-spot swimming crab Portunus sanguinolentus (Herbst, 1783) shell from the coasts of Sindh and Balochistan, Pakistan.

The present study aimed to investigate the elemental composition of the hard shell of the three-spot swimming crab, Portunus sanguinolentus, collected from the coasts of Pakistan. Thirty crab shells were collected and divided into three groups considering their size. The element detection was performed by energy-dispersive X-ray spectroscopy with scanning electron microscope (SIM/EDAX). The mean concentration of carbon, oxygen, calcium, copper, magnesium, and phosphorus observed in the shell of P. sanguinolentus was 13.63 ± 6.21%, 46.25 ± 12.62%, 34.39 ± 18.33%, 3.19 ± 1.25%, 1.15 ± 0.99%, and 1.39 ± 0.51%, respectively. The concentration pattern of these observed elements in P. sanguinolentus shells was found in the following order: O > Ca > C > Cu > P > Mg. To compare among the three groups of shells, the highest concentration of calcium (54.60%) was obtained in large-sized shell groups, which was significantly different from the other groups (p < 0.05). A high concentration of copper was found to be accumulated in the large-sized (3.55%) and medium-sized (4.21%) shell groups, which was significantly higher than in small-sized shell groups (p < 0.05). In the case of the large-sized shell group, the mean concentrations of magnesium and phosphorus were significantly lower than the medium-sized shell group (p < 0.05). The results indicate that crab shells could act as a good bio-sorbent for several minerals in its ecosystem. P. sanguinolentus shell is very rich in calcium, magnesium, and phosphorus. Extraction of calcium, magnesium, and phosphorus from P. sanguinolentus shells could be profitable for biofertilizer and pharmaceutical industries.