Mygalin, an Acanthoscurria gomesiana spider-derived synthetic, modulates haloperidol-induced cataleptic state in mice.

BACKGROUND: Haloperidol (HAL) is an antipsychotic used in the treatment of schizophrenia. However, adverse effects are observed in the extrapyramidal tracts due to its systemic action. Natural compounds are among the treatment alternatives widely available in Brazilian biodiversity. Mygalin (MY), a polyamine that was synthesized from a natural molecule present in the hemolymph of the Acanthoscurria gomesian spider, may present an interesting approach. AIMS: This study aimed to evaluate the effect of MY in mice subjected to HAL-induced catalepsy. METHODS: Male Swiss mice were used. Catalepsy was induced by intraperitoneal administration of HAL (0.5 mg/kg - 1 mL/Kg) diluted in physiological saline. To assess the MY effects on catalepsy, mice were assigned to 4 groups: (1) physiological saline (NaCl 0.9 %); (2) MY at 0.002 mg/Kg; (3) MY at 0.02 mg/Kg; (4) MY at 0.2 mg/Kg. MY or saline was administered intraperitoneally (IP) 10 min b HAL before saline. Catalepsy was evaluated using the bar test at 15, 30, 60, 90, and 120 min after the IP administration of HAL. RESULTS: The latency time in the bar test 15, 30, 60, and 90 min increased (p < 0.05) after IP administration of HAL compared to the control group. Catalepsy was attenuated 15, 30, 90, and 120 min (p < 0.05) after the IP-administration of MY at 0.2 mg/Kg; while MY at 0.02 mg/Kg attenuated catalepsy 15 min after the HAL treatment. Our findings showed that MY attenuates the HAL-induced cataleptic state in mice.

Mygalin, an Acanthoscurria gomesiana spider‐derived synthetic, modulates haloperidol-induced cataleptic state in mice

Background: Haloperidol (HAL) is an antipsychotic used in the treatment of schizophrenia. However, adverse effects are observed in the extrapyramidal tracts due to its systemic action. Natural compounds are among the treatment alternatives widely available in Brazilian biodiversity. Mygalin (MY), a polyamine that was synthesized from a natural molecule present in the hemolymph of the Acanthoscurria gomesian spider, may present an interesting approach. Aims: This study aimed to evaluate the effect of MY in mice subjected to HAL-induced catalepsy. Methods: Male Swiss mice were used. Catalepsy was induced by intraperitoneal administration of HAL (0.5 mg/kg − 1 mL/Kg) diluted in physiological saline. To assess the MY effects on catalepsy, mice were assigned to 4 groups: (1) physiological saline (NaCl 0.9 %); (2) MY at 0.002 mg/Kg; (3) MY at 0.02 mg/Kg; (4) MY at 0.2 mg/Kg. MY or saline was administered intraperitoneally (IP) 10 min b HAL before saline. Catalepsy was evaluated using the bar test at 15, 30, 60, 90, and 120 min after the IP administration of HAL. Results: The latency time in the bar test 15, 30, 60, and 90 min increased (p < 0.05) after IP administration of HAL compared to the control group. Catalepsy was attenuated 15, 30, 90, and 120 min (p < 0.05) after the IP-administration of MY at 0.2 mg/Kg; while MY at 0.02 mg/Kg attenuated catalepsy 15 min after the HAL treatment. Our findings showed that MY attenuates the HAL-induced cataleptic state in mice.

Effect of lower limb resistance training on ICF components in chronic stroke: A systematic review and meta-analysis of RCTs.

BACKGROUND: Resistance training (RT) effectively promotes functional independence after stroke. OBJECTIVES: To investigate the effect of lower limb RT on body structure and function (muscle strength, postural balance), activity (mobility, gait) and participation (quality of life, impact of stroke on self-perceived health) outcomes in individuals with chronic stroke. METHODS: Six databases were searched from inception until September 2022 for randomized controlled trials comparing lower limb RT to a control intervention. The random-effects model was used in the meta-analyses. Effect sizes were reported as standardized mean differences (SMD). Quality of evidence was assessed using the GRADE approach. RESULTS: Fourteen studies were included. Significant improvements were found in body structure and function after lower limb RT: knee extensors (paretic side - SMD: 1.27; very low evidence), knee flexors (paretic side - SMD: 0.51; very low evidence; non-paretic side - SMD: 0.52; low evidence), leg press (paretic side - SMD: 0.83; very low evidence) and global lower limb muscle strength (SMD: -1.47; low evidence). No improvement was found for knee extensors (p = 0.05) or leg press (p = 0.58) on the non-paretic side. No improvements were found in the activity domain after lower limb RT: mobility (p = 0.16) and gait (walking speed-usual: p = 0.17; walking speed-fast: p = 0.74). No improvements were found in the participation domain after lower limb RT: quality of life (p > 0.05), except the bodily pain dimension (SMD: 1.02; low evidence) or the impact of stroke on self-perceived health (p = 0.38). CONCLUSION: Lower limb RT led to significant improvements in the body structure and function domain (knee extensors and flexors, leg press, global lower limb muscle strength) in individuals with chronic stroke. No improvements were found in the activity (mobility, gait [walking speed]) or participation (quality of life, impact of stroke on self-perceived health) domains. PROSPERO REGISTRATION NUMBER: CRD42021272645.

Do indesejável ao (in)evitável: velhice estigmatizada na pandemia da Covid-19 / From the undesirable to the (in) preventable: old age stigmatized in the Covid-19 pandemic / De lo indeseable a lo (in) evitable: vejez estigmatizada enla pandemia de Covid-19

O artigo tem por objetivo analisar as representações sociais sobre a imagem da pessoa idosa associadas a pandemia de COVID-19 nas publicações do Instagram. Caracteriza-se como um estudo exploratório, descritivo, de abordagem qualitativa, com estratégia de investigação pautada no estudo de caso. Os dados consistiram em 19 memes publicados na internet na rede social Instagram por meio da busca aleatória do maior número possível de memes, a partir do buscador "idoso" e "quarentena". A partir da Técnica de Análise de Conteúdo proposta por Bardin emergiram duas categorias analíticas: Categoria 1. Recolhimento/enclausuramento da pessoa idosa (52,63%) e Categoria 2. Impedimento/limitação da mobilidade das pessoas idosas fora de casa (47,37%). As representações sociais acerca da pessoa idosa publicadas nas redes sociais relacionadas ao caso da COVID-19 desvelaram um sentido negativo atribuído ao envelhecer e demonstram como há um processo de naturalização da estigmatização e invisibilidade da violência contra pessoas idosas. (AU)

The article aims to analyze the social representations of the image of the elderly associated with the COVID-19 pandemic in Instagram publications. It is characterized as an exploratory, descriptive study with a qualitative approach, with an investigation strategy based on the case study.The data consisted of 19 memes published on the internet on the social network Instagramthrough the random search of the largest possible number of memes, using the search engine "elderly" and "quarantine". From the Content Analysis Technique proposed by Bardin, two analytical categories emerged: Category 1. Withdrawal/enclosure of the elderly person (52.63%) and Category 2. Impediment/limitation of mobility of elderly people outside the home (47.37 %). The social representations about the elderly person published on social networks related to the case of COVID-19 revealed a negative meaning attributed to aging and demonstrate how there is a process of naturalization of stigmatization and invisibility of violence against the elderly. (AU)

El artículo tiene como objetivo analizar las representaciones sociales de la imagen del anciano asociado a la pandemia COVID-19 en publicaciones de Instagram. Se caracteriza por ser un estudio exploratorio, descriptivo con enfoque cualitativo, con una estrategia de investigación basada en el estudio de caso. Los datos consistieron en 19 memes publicados en Internet en la red social Instagram através de la búsqueda aleatoria del mayor número posiblede memes, utilizando el motor de búsqueda "ancianos" y "cuarentena". De la Técnica de Análisis de Contenidos propuesta por Bardin surgieron dos categorías analíticas: Categoría 1. Retiro/encerramiento del anciano (52,63%) y Categoría 2. Impedimento/limitación de la movilidad de los ancianos fuera del hogar (47,37%). Las representaciones sociales sobre las personas mayores publicadas en las redes sociales relacionadas con el caso de COVID-19 revelaron un significado negativo atribuido al envejecimiento y demuestran cómo hay un proceso de naturalización de la estigmatización e invisibilidad de la violencia contra las personas mayores. (AU)

Effects of a 28-day feeding trial of grain-containing versus pulse-based diets on cardiac function, taurine levels and digestibility in domestic dogs.

In 2018, the US Food and Drug Administration reported a link between canine dilated cardiomyopathy (DCM) and grain-free diets. Evidence to support a link has emerged, but the specific ingredients responsible and the role of taurine or other causative factors remain unclear. We hypothesized dogs fed pulse-based, grain-free diets for 28 days will show decreased macronutrient digestibility, increased fecal bile acid excretion, and reduced plasma cystine, cysteine, methionine and taurine, causing sub-clinical cardiac or blood changes indicative of early DCM. Three diets were formulated using white rice flour (grain), whole lentil (grain-free), or wrinkled pea (grain-free) and compared to the pre-trial phase on a commercial grain-based diet. After 28 days of feeding each diet, the wrinkled pea diet impaired stroke volume and cardiac output, increased end-systolic ventricular diameter and increased plasma N-Terminal Pro-B-type Natriuretic Peptide (NT-ProBNP), albeit in a sub-clinical manner. Digestibility of some macronutrients and sulphur-containing amino acids, excluding taurine, also decreased with pulse-based compared to grain-based diets, likely due to higher fiber levels. Plasma taurine levels were unchanged; however, plasma methionine was significantly lower after feeding all test diets compared to the commercial diet. Overall, DCM-like changes observed with the wrinkled pea diet, but not lentil diet, after only 4 weeks in a breed not known to be susceptible support a link between pea-based diets and canine nutritionally-mediated DCM.

Analysis of Plantar Tactile Sensitivity in Older Women after Conventional Proprioceptive Training and Exergame.

OBJECTIVE: To evaluate and compare the effects of conventional proprioceptive training and games with motion monitoring on plantar tactile sensitivity in older women. METHODS: A randomized controlled clinical trial, with 50 older women randomized into three groups: conventional proprioception (n = 17), games with motion monitoring (n = 16), and the control (n = 17). They underwent 24 intervention sessions, three times a week, for eight weeks. The conventional proprioception group performed exercises involving gait, balance, and proprioception. The games performed by the motion monitoring group included exercises using the Xbox Kinect One video game from Microsoft®. The evaluation of tactile pressure sensitivity was performed using Semmes-Weinstein monofilaments. Intragroup comparisons between the two paired samples were performed using paired Student's t-test or Wilcoxon test. Intergroup comparisons between the three independent samples were performed using the Kruskal-Wallis test and Dunn's post hoc test, with p ≤ 0.05. RESULTS: The older women submitted to conventional games with motion monitoring training and showed improvement in plantar tactile sensitivity in the right and left feet. When comparing the intergroup results, the two training modalities obtained an improvement in the plantar tactile sensitivity of the older women when compared to the control group. CONCLUSIONS: We conclude that both training modalities may favor the improvement of plantar tactile sensitivity in older women, with no significant differences between conventional and virtual training.

Children and youth mental health from the perspective of workers in a health network / Saúde mental infantojuvenil na perspectiva de trabalhadores de uma rede de saúde / La salud mental infantil y juvenol desde la perspectiva de los trabajadores de una red sanitaria

The objective was to understand the difficulties and potential of a Mental Health (MH) network aimed at children and adolescents, identifying how professionals understand and operationalize the principles of the policy of SM for children and adolescents. Five Focus Groups were held, with the participation of 43 workers. We used lexical analysis through the Iramuteq software, originating four classes: "What is being a child and adolescent?"; "What do children and adolescents suffer from?"; "On the relationship between services" and "Potentials and challenges of the Mental Health Network". Difficulties in sharing care were identified, articulating the network outside of oneself. Capsij is perceived as a priority for user embracement, and there is still difficulty in providing care in MH in Primary Care (AB). It is concluded that the complexity of care in MS for children and adolescents imposes the need to continue strategies to strengthen the AB and intersectoral actions aimed at an expanded clinic. (AU)

Objetivou-se conhecer as dificuldades e potencialidades de uma rede de Saúde Mental (SM) voltada a crianças e adolescentes, identificando como profissionais compreendem e operacionalizam os princípios da política de SM infantojuvenil. Foram realizados cinco Grupos Focais, com participação de 43 trabalhadores. Utilizou-se da análise lexical por meio do software Iramuteq, originando quatro classes: "O que é ser criança e adolescente?"; "Do que sofrem as crianças e adolescentes?"; "Sobre a relação entre os serviços" e "Potencialidades e desafios da Rede de Saúde Mental". Identificou-se dificuldades em compartilhar o cuidado, articulando a rede para fora de si. O Capsij é percebido como prioritário para o acolhimento, havendo, ainda, dificuldade em efetivar os cuidados em SM na Atenção Básica (AB). Conclui-se que a complexidade da atenção em SM para crianças e adolescentes impõe a necessidade de continuidade de estratégias de fortalecimento da AB e de ações intersetoriais visando uma clínica ampliada. (AU)

El objetivo fue comprender las dificultades y potencialidades de una red de Salud Mental (SM) para niños y adolescentes, identificando cómo los profesionales entienden y operacionalizan los principios de la política de SM para la niñez y adolescencia. Se realizaron cinco Grupos Focales, en los que participaron 43 trabajadores. Se utilizó el análisis léxico a través del software Iramuteq, originando cuatro clases: "¿Qué es ser niño y adolescente?"; "¿Qué padecen los niños y adolescentes?"; "Sobre la relación entre servicios" y "Potencialidades y desafíos de la Red de Salud Mental". Se identificaron dificultades a la hora de compartir los cuidados, articulando la red para fuera de sí. El Capsij se percibe como una prioridad para la acogida, existiendo aún dificultades para implementar los cuidados en SM en Atención Primaria (AP). Se concluye que la complejidad de la atención en SM para niños y adolescentes impone la necesidad de continuidad de las estrategias de fortalecimiento de la AP y de acciones intersectoriales, visando una clínica ampliada. (AU)

Difficulties in Caring for the Older Adults: Perspective of Brazilian and Portuguese Caregivers.

This study aims to understand the difficulties in caring for the older adults with functional dependence from the perspective of Brazilian and Portuguese caregivers. This is a study based on the Theory of Social Representations, based on the Thematic Content Analysis proposed by Bardin, carried out with 21 informal caregivers of older adults in Brazil and 11 informal caregivers of older adults in Portugal. The instrument consisted of a questionnaire with sociodemographic data and data on health conditions along with an open interview with guiding questions on the theme of care. Data were analyzed using the Content Analysis technique proposed by Bardin, with the help of the QRS NVivo® Version 11 software (QSR International, Burlington, MA, USA). Three categories emerged from the speeches: "Caregiver burden", "Caregiver support network" and "Older adults resistance". The main difficulties mentioned by caregivers were associated with family articulation in meeting the needs of their older adults, whether due to the excessive demand of tasks, which results in overloading the caregiver, or the behaviors of the older adults themselves, or even the availability of a truly supportive and effective network.

Managing inpatient bed setup: an action-research approach using lean technical practices and lean social practices.

PURPOSE: This article aims to introduce a guide to improving hospital bed setup by combining lean technical practices (LTPs), such as kaizen and value stream mapping (VSM) and lean social practices (LSPs), such as employee empowerment. DESIGN/METHODOLOGY/APPROACH: Action research approach was employed to analyze the process of reconfiguration of bed setup management in a Brazilian public hospital. FINDINGS: The study introduces three contributions: (1) presents the use of VSM focused specifically on bed setup, while the current literature presents studies mainly focused on patient flow management, (2) combines the use of LSPs and LTPs in the context of bed management, expanding current studies that are focused either on mathematical models or on social and human aspects of work, (3) introduces a practical guide based on six steps that combine LSPs and LSPs to improve bed setup management. RESEARCH LIMITATIONS/IMPLICATIONS: The research focused on the analysis of patient beds. Surgical beds, delivery, emergency care and intensive care unit (ICU) were not considered in this study. In addition, the process indicators analyzed after the implementation of the improvements did not contemplate the moment of the COVID-19 pandemic. Finally, this research focused on the implementation of the improvement in the context of only one Brazilian public hospital. PRACTICAL IMPLICATIONS: The combined use of LSPs and LTPs can generate considerable gains in bed setup efficiency and consequently increase the capacity of a hospital to admit new patients, without the ampliation of the physical space and workforce. SOCIAL IMPLICATIONS: The improvement of bed setup has an important social character, whereas it can generate important social benefits such as the improvement of the admission service to patients, reducing the waiting time, reducing hospitalization costs and improving the hospital capacity without additional physical resources. All these results are crucial for populations, their countries and regions. ORIGINALITY/VALUE: While the current literature on bed management is more focused on formal models or pure human and social perspectives, this article brings these two perspectives together in a single, holistic framework. As a result, this article points out that the complex bed management problem can be efficiently solved by combining LSPs and LTPs to present theoretical and practical contributions to the important social problem of hospital bed management.

Percepções de familiares sobre uma rede de cuidados de saúde mental infantojuvenil / Family perceptions about a children's mental health network / Percepciones familiares sobre una red de atención de salud mental infantojuvenil

Este artigo pretende conhecer como a rede de cuidados em saúde tem se operacionalizado a partir da percepção de familiares de crianças com demanda de cuidado em saúde mental (SM). Foram realizados dois grupos focais, um com familiares da Atenção Básica (AB) e outro com familiares do Centro de Atenção Psicossocial Infantojuvenil (CAPSij), totalizando 15 participantes. Seguiu-se com a análise lexical do tipo classificação hierárquica descendente, com o auxílio do software R Interface, a fim de análises multidimensionais de textos e questionários (IRaMuTeQ), resultando em cinco classes: A Pílula Mágica; Forças e Fraquezas dos serviços; Procurando por ajuda; Aceitando o diagnóstico da criança e Onde procurei ajuda. Os resultados apontam para dificuldades presentes na AB em identificar e manejar situações de Saúde Mental Infantojuvenil (SMIJ), por meio de uma lógica ainda medicalizante. Ressalta-se que a escola é apresentada como lugar de destaque na produção da demanda por cuidado e a família ainda é pouco convocada à construção das ações. Conclui-se, então, que avanços ainda são necessários para operacionalização de um cuidado pautado nas diretrizes da política de SMIJ.(AU)

This article aims to know how the healthcare network has been operationalized from the perception of family members of children with demand for mental health care (MH). Two focus groups were held, one with family members from Primary Care (PC) and the other with family members from the Child Psychosocial Care Center (CAPSij), totaling 15 participants. A lexical analysis of the descending hierarchical classification type was performed with the help of the software R Interface for multidimensional analyzes of texts and questionnaires (IRAMUTEQ), resulting in five classes: The Magic Pill; Strengths and Weaknesses of services; Looking for help; Accepting the child's diagnosis; and Where did I look for help. The results point to difficulties present in PC in identifying and managing situations of mental health in children and adolescents (MHCA), with a medicalization logic. Note that the school is presented as a prominent place in producing the demand for care, and the family is still not very much involved in the actions. It is, thus, concluded that advances are still needed for operationalization of care guided by MHCA policy guidelines.(AU)

Este artículo tuvo por objetivo conocer cómo opera una red asistencial a partir de la percepción de familiares de niños con demanda de atención en salud mental (SM). Se realizaron dos grupos focales, uno con familiares de Atención Primaria (AP) y otro con familiares del Centro de Atención Psicosocial Infantojuvenil (CAPSij), totalizando 15 participantes. Se realizó análisis léxico del tipo clasificación jerárquica descendente con la ayuda del software Interface de R pour les Analyses Multidimensionnelles de Textes et de Questionnaires (IRAMUTEQ), lo que resultó en cinco clases: "La píldora mágica"; "Fortalezas y debilidades de los servicios"; "En busca de ayuda"; "Aceptar el diagnóstico del niño" y "¿Dónde busqué ayuda?". Los resultados apuntan las dificultades presentes en AP para identificar y manejar situaciones de salud mental infantojuvenil (SMIJ) mediante una lógica aún medicalizante. La escuela tiene un lugar destacado en la producción de la demanda de cuidados y la familia aún no está muy involucrada en la construcción de acciones. Se concluye que se necesitan avances para ofertar una atención guiada por lineamientos de la política del SMIJ.(AU)

Existe correlação entre atividade elétrica muscular e cerebral após treino com terapia de contensão do membro superior e tronco em sujeitos com acidente vascular encefálico? / Is there a correlation between muscle and brain electrical activity after training with upper limb and trunk constraint-induced therapy in subjects with stroke?

Objetivo: Verificar se existe correlação entre excitabilidade central e periférica após o treino com a terapia de restrição induzida do movimento (TRIM) modificada associada a contensão de tronco para o membro superior parético após Acidente Vascular Encefálico (AVE). Métodos: Ensaio clínico controlado onde vinte e dois voluntários foram selecionados e randomizado em dois grupos: Grupo Controle (GC) e Grupo Experimental (GE). Eles foram avaliados por meio do eletroencefalograma (EEG) e da eletromiografia (EMG) de superfície. Os canais EEG analisados foram: Canal Ântero Frontal (AF3/AF4); Canal Frontal região medial (F7/F8); Canal Frontal região lateral (F3/F4); Canal Fronto Central (FC5/FC6); Região temporal (T7/T8); Região occipital (O1/O2). Os músculos analisados com EMG foram bíceps braquial, flexores e extensores de punho. As avaliações foram realizada durante a atividade, por dez minutos. O GE recebeu o treinamento com TRIM modificada uma hora por dia, duas semanas consecutivas. Resultados: O GC (pós-intervenção) apresentou correlação moderada negativa (r= -0,69; p= 0,02) do canal Fa com extensor de punho. O GE apresentou (pré-intervenção) correlação moderada negativa do canal AF (r= -0,68; p= 0,02) e FC (r= -0,71; p= 0,01) com flexor de punho. Na pós intervenção apresentou correlação positiva do canal Fa (r= 0,61; p= 0,04) com o extensor de punho. Conclusão: A TRIM modificada associada à contensão de tronco para o membro superior parético apresentou correlação positiva do canal Fa com o músculo extensor de punho e o grupo não treinado apresentou correlação negativa do canal Fa com extensor de punho.

Objective: Establish the correlation between central and peripheral excitability after training with the modified upper limb constraint-induced movement therapy (CIMT) associated with trunk constraint for patients with stroke. Methods: This study is a randomized clinical trial. Twenty-two volunteers were included and randomized into the Control (CG) and Experimental Groups (EG). They were assessed with electroencephalography (EEG) and surface electromyography (EMG). The EEG channels analyzed were antero-frontal (AF3/AF4), frontal-medial (F7/F8), frontal-lateral (F3/F4), frontal-central (FC5/FC6), temporal (T7/T8), and occipital (O1/O2). The muscles evaluated with EMG were biceps brachii, wrist flexors, and wrist extensors. The evaluations were performed simultaneously with a functional assessment for ten minutes. The EG received the modified CIMT training one hour per day for two consecutive weeks. Results: After the intervention, the CG showed a moderate negative correlation of the Fa channel with the wrist extensor (r= -0.69; p= 0.02), whereas the EG had a moderate negative correlation of the FA channel (r= -0.68; p= 0.02) and FC (r= - 0.71; p= 0.01) with wrist flexor. In the post-intervention, a positive correlation of the FA channel was found with the wrist extensor (r= 0.61; p= 0.04). Conclusion: The modified CIMT associated with the trunk constraint for the paretic upper limb showed a positive correlation between the Fa channel and the wrist extensor muscle, and the control group showed a negative correlation between the Fa channel and the wrist extensor.

Effect of Foot Reflexology on Muscle Electrical Activity, Pressure, Plantar Distribution, and Body Sway in Patients with Type 2 Diabetes Mellitus: A Pilot Randomized Controlled Trial.

OBJECTIVE: To verify the effect of foot reflexology on the electrical muscle activity of the lateral and medial gastrocnemius muscle, and to examine the distribution, plantar pressure, and body sway in patients with type 2 diabetes mellitus. METHODS: This pilot randomized controlled trial enrolled 17 volunteers who were clinically diagnosed with diabetes mellitus. The sample was assigned to one of two groups: the control group (CG, n = 7), who received information on foot care and health, and the intervention group (IG, n = 10), who received the application of foot reflexology on specific areas of the feet, for 10 consecutive days. There was blinding of the evaluator and the therapist. Surface electromyography (EMG) was used to assess the electrical activity of the medial and lateral gastrocnemius muscles in maximum voluntary isometric contraction (MVIC) and isotonic contraction (IC); baropodometry and stabilometry were used to analyze unloading, plantar weight distribution, and body sway. RESULTS: There was a statistically significant difference for the variables of maximum peak electrical activity of the left medial gastrocnemius (p = 0.03; effect size = 0.87 and power = 0.81) and left lateral gastrocnemius muscles (p = 0.04, effect size = 0.70 and power = 0.66) respectively, in the intragroup IC, and median frequency of the left medial gastrocnemius muscle in the intragroup MVIC (p = 0.03; effect size = 0.64 and power = 0.59), and in the variables intergroups of the total area on the right side (p = 0.04; effect size = 1.03 and power = 0.50) and forefoot area on the left side (p = 0.02; effect size = 0.51 and power = 0.16). CONCLUSIONS: We conclude that foot reflexology influenced some variables of the intergroup plantar distribution and intragroup EMG in the sample studied. There is a need for a placebo group, a larger sample and a follow-up to strengthen the findings of these experiments.

Health education actions on male breast cancer: A protocol for systematic review and meta analysis.

BACKGROUND: Although rare, the rates of diagnosis of male breast cancer (MBC) have been increasing over the years, and it can be quite aggressive in the male organism. Some strategies such as health education disseminating knowledge about MBC can be essential for early discovery and finding better prognosis. OBJECTIVE: Protocol to map the available evidence of health education approaches on MBC. METHODS: A scoping review on health education on MBC will be carried out in Latin American and Caribbean Literature on Health Sciences (LILACS), Web of Science, Scopus, Scielo, Online System of Literature Search and Analysis Medical (MEDLINE), Embase, Virtual Health Library (VHL). Two independent reviewers will perform screening, data extraction, and risk of bias assessment through the Joanna Briggs Institute (JBI) Critical Assessment Checklist. For the quality of evidence, the Grading of Recommendations, Assessment, Development and Evaluation (GRADE) Analysis will be used. RESULTS: The results of this review will be published in a peer-reviewed journal. CONCLUSIONS: This scoping review will provide evidence of how health education on MBC is being addressed in health systems. Evidence can help healthcare professionals and patients recognize the most effective educational inventions in disseminating knowledge and preventing MBC.

Elderly People's Memories about the Itinerary of the HIV/AIDS Diagnosis.

This study aimed to analyze the remembrances of elderly people about their illness and the path taken in health services until diagnosis of HIV/AIDS. This is a cross-sectional and descriptive analytical study, with a qualitative approach and theoretical and methodological support from the social representations theory and conducted with 38 elderly people. A questionnaire with sociodemographic data, health conditions, and a script for semi-structured interviews was used. Data analysis was performed with the support of the QRS NVivo® software and in light of Bardin's content analysis. In relation to the itinerary to the diagnosis of HIV/AIDS, the memories are from manifestations of opportunistic diseases and of a long trajectory with health problems and hospitalizations in search of a late diagnosis. The representation of death associated with the diagnosis of HIV/AIDS materializes for some with the loss of the partner in this process of searching for the diagnosis.

Influenza A(H11N2) Virus Detection in Fecal Samples from Adélie (Pygoscelis adeliae) and Chinstrap (Pygoscelis antarcticus) Penguins, Penguin Island, Antarctica.

Influenza A viruses infect a range of host species, including a large variety of mammals and more than a hundred species of birds. A total of 95 avian fecal samples were collected from penguin colonies in the South Shetland Islands, close to the Antarctic Peninsula, and tested by reverse transcription-PCR (RT-PCR) to detect avian influenza viruses (AIVs). Five out of seven samples collected from Penguin Island were positive for AIVs. Analysis of the genomes recovered from four samples revealed the detection of influenza A(H11N2) virus in fecal samples from Adélie penguins (Pygoscelis adeliae) and from a colony of chinstrap penguins (Pygoscelis antarcticus). Bayesian phylogeographic analysis revealed the clustering of all currently available H11N2 samples from Antarctica's avifauna in a single cluster that emerged at least in the early 2010s, suggesting its continued circulation on the continent. Our results reinforce the need for continuous surveillance of avian influenza on the Antarctic continent. IMPORTANCE Although wild birds play a role in the transmission and ecology of avian influenza viruses (AIVs) across the globe, there are significant gaps in our understanding of the worldwide distribution of these viruses in polar environments. In this study, using molecular analysis and full-genome sequencing, we describe the detection of distinct influenza A(H11N2) viruses in fecal samples of penguins in the Southern Shetland Islands, Antarctica. We emphasize the need for virus monitoring as AIVs may have implications for the health of endemic fauna and the potential risk of the introduction of highly pathogenic AIVs to the continent.

Conception of workers in a municipal health network about the service-management relationship / Concepção dos Trabalhadores de uma Rede Municipal de Saúde sobre a Relação Serviço-Gestão / Concepción de los trabajadores de una red de salud municipal sobre la relación servicio-gestión

The objective of this work was to understand the conceptions of health professionals about the relationship between management and the different services that constitute the public health network of a municipality in the southeastern region of Brazil. It used the Focus Group (FG) technique for data collection. Three FG were held with an average of 12 participants per meeting, totaling 38 participants. The Lexical Descending Hierarchical Classification analysis was applied with the support of the Iramuteq software. The results outlined four classes: Management-Service Relationship; Daily challenges of services; Sustainability of work in the territory and Prioritization of the user. The relationship between health services and management was marked by hierarchical practices. However, it was possible to verify that there are expectations regarding the establishment of a transversal relationship. Collective spaces and user participation in care management are considered as important elements for co-management. (AU)

Objetivou-se compreender as concepções de profissionais de saúde sobre a relação de uma gerência com os diversos serviços que compõem a rede de saúde pública de um município da região sudeste do Brasil. Utilizou-se da técnica do Grupo Focal (GF) para a coleta de dados. Foram realizados três GF com a composição média de 12 participantes por encontro, totalizando 38 participantes. Seguiu-se com a análise lexical do tipo Classificação Hierárquica Descendente com o auxílio do software Iramuteq. Os resultados delinearam quatro classes: "Relação Gestão-Serviço"; "Desafios cotidianos dos serviços"; "Sustentação do trabalho no território" e "Priorização do usuário". O estudo indica que a relação serviços-gestão é marcada por práticas hierarquizadas. Contudo, foi possível verificar que existem expectativas em torno da instauração de uma relação transversalizada. Pondera-se os espaços coletivos e a participação dos usuários na gestão do cuidado como elementos importantes para a cogestão. (AU)

El objetivo de este trabajo fue comprender las concepciones de los profesionales de la salud sobre la relación entre la gestión y los diferentes servicios que componen la red pública de salud de un municipio de la región sudeste de Brasil. Se utilizó la técnica de Grupo Focal (GF) para la recolección de datos. Se realizaron tres GF con un promedio de 12 participantes por reunión, totalizando 38 participantes. Un análisis léxico de Clasificación Jerárquica Descendente fue aplicado con el apoyo del software Iramuteq. Los resultados distinguieron cuatro clases: "Relación Gestión-Servicio"; "Retos cotidianos del servicio"; "Sostenibilidad del trabajo en el territorio" y "Priorización de usuario". El estudio indicó que la relación entre los servicios de salud y la gestión está marcada por prácticas jerárquicas. Sin embargo, se pudo constatar que existen expectativas en cuanto al establecimiento de una relación transversal. Los espacios colectivos y la participación de los usuarios en la gestión de la atención se consideran elementos importantes para la cogestión. (AU)

Monitoring Stimulated Darkening from UV-C Light on Different Bean Genotypes by NMR Spectroscopy.

The use of UV-C cool white light on bean (Phaseolus vulgaris L.) seeds significantly increases the biochemical seed coat post-harvest darkening process, whilst preserving seed germination. The aim of this work consists in monitoring the effect caused by the incidence of UV-C light on different bean genotypes using NMR spectroscopy. The genotype samples named IAC Alvorada; TAA Dama; BRS Estilo and BRS Pérola from the Agronomic Institute (IAC; Campinas; SP; Brazil) were evaluated. The following two methodologies were used: a prolonged darkening, in which the grain is placed in a room at a controlled temperature (298 K) and humidity for 90 days, simulating the supermarket shelf; an accelerated darkening, where the grains are exposed to UV-C light (254 nm) for 96 h. The experiments were performed using the following innovative time-domain (TD) NMR approaches: the RK-ROSE pulse sequence; one- and two-dimensional high resolution (HR) NMR experiments (1H; 1H-1H COSY and 1H-13C HSQC); chemometrics tools, such as PLS-DA and heat plots. The results suggest that the observed darkening occurs on the tegument after prolonged (90 days) and accelerated (96 h) conditions. In addition, the results indicate that phenylalanine is the relevant metabolite within this context, being able to participate in the chemical reactions accounted for by the darkening processes. Additionally, it is possible to confirm that a UV-C lamp accelerates oxidative enzymatic reactions and that the NMR methods used were a trustworthy approach to monitor and understand the darkening in bean seeds at metabolite level.

Obesidade e fatores de risco associados em idosos residentes no interior da Bahia / Obesity and associated risk factors in elderly residents in the interior of Bahia

Avaliar a obesidade e os fatores de riscos associados à obesidade em idosos residentes no interior da Bahia. Trata-se de uma pesquisa do tipo exploratória, descritiva e analítica, com delineamento transversal e abordagem quantitativa. O instrumento de pesquisa foi constituído de dados sociodemográficos e condições de saúde. Constatou-se maior frequência de idosos do sexo feminino (80,0%), faixa etária de 60 a 69 anos (50,0%), viúvos (40,9%), renda de um salário mínimo (55,5%) e alfabetizados (86,4%). Em relação à capacidade funcional, a maioria dos idosos foi classificada como independente nas Atividades Básicas de Vida Diária (83,6%) e Atividades Instrumentais de Vida Diária (70,9%). No que se refere ao estado nutricional, segundo o IMC, 50,0% dos idosos apresentaram a condição de excesso de peso (>27 kg/m²). Com aplicação do teste Qui-quadrado (χ2) entre as todas categorias do IMC e as variáveis do estudo verificou-se diferença estatisticamente significante a categoria excesso de peso (>27 kg/m2) e as variáveis do estudo: HAS (p=0,043), presença de doença (p=0,019) e sexo feminino (p=0,000). Constatou-se alta frequência de sobrepeso e doenças crônicas relacionadas ao sobrepeso no grupo de idosos pesquisados, sendo a hipertensão arterial sistêmica e o diabetes as mais frequentes.

To evaluate obesity and the risk factors associated with obesity in elderly people living in the interior of Bahia. This is an exploratory, descriptive and analytical research, with a cross-sectional design and quantitative approach. The research instrument consisted of socio-demographic data and health conditions. There was a higher frequency of elderly women (80.0%), aged between 60 and 69 years (50.0%), widowed (40.9%), income of 1 minimum wage (55, 5%) and literate (86.4%). Regarding functional capacity, most elderly people were classified as independent in Basic Activities of Daily Living (83.6%) and Instrumental Activities of Daily Living (70.9%). With regard to nutritional status, according to the BMI, 50.0% of the elderly people were overweight (> 27 kg / m²). With the application of the chi-square test (χ2) between all categories of BMI and the study variables, there was a statistically significant difference between the category of overweight (> 27 kg / m2) and the study variables: SAH (p=0.043), presence of disease (p=0.019) and female gender (p=0.000). There was a high frequency of overweight and chronic diseases related to overweight in the group of elderly people surveyed, with systemic arterial hypertension and diabetes being the most frequent.

Central involvement of 5-HT1A receptors in antinociception induced by photobiomodulation in animal model of neuropathic pain.

This study aimed to investigate the central involvement of 5-HT1A receptors in the nociceptive behavior of mice submitted to the chronic constriction injury (CCI) of sciatic nerve and the subsequent application of photobiomodulation (PBM). Male mice (Swiss-albino) were submitted to CCI and subsequently received an infusion of WAY100635 (5-HT1A receptor antagonist) or intracerebroventricular saline (ICV), followed by infrared laser irradiation (808 nm), in continuous mode, with the power of 100 mW and a dose of 0 J/cm2 (control group) or 50 J/cm2. The thermal hyperalgesia was evaluated by hot plate test, while mechanical allodynia was evaluated by von Frey filaments. After CCI, animals showed a reduction in the nociceptive threshold (p<0.001) when compared to the sham group. In von Frey test, the CCI + saline + PBM 50 J/cm2 group showed an increase in nociceptive threshold (p<0.001) in all measurement moments in comparison with groups CCI + SALINE + PBM 0 J/cm2, CCI + WAY100635 + PBM 50 J/cm2, and CCI + WAY100635 + PBM 0 J/cm2. Similarly, in hot plate test, CCI + SALINE + PBM 50 J/cm2 group showed an increase in nociceptive threshold after application of PBM at 120 and 180 min. Because of the results found, it can be suggested the involvement of 5-HT1A receptors in the central nervous system, since WAY100635 was able to reverse the antinociceptive effect provided by PBM in animals submitted to CCI.

Genetic mapping reveals complex architecture and candidate genes involved in common bean response to Meloidogyne incognita infection.

Root-knot nematodes (RKNs), particularly Meloidogyne incognita, are among the most damaging and prevalent agricultural pathogens due to their ability to infect roots of almost all crops. The best strategy for their control is through the use of resistant cultivars. However, laborious phenotyping procedures make it difficult to assess nematode resistance in breeding programs. For common bean, this task is especially challenging because little has been done to discover resistance genes or markers to assist selection. We performed genome-wide association studies and quantitative trait loci mapping to explore the genetic architecture and genomic regions underlying the resistance to M. incognita and to identify candidate resistance genes. Phenotypic data were collected by a high-throughput assay, and the number of egg masses and the root-galling index were evaluated. Complex genetic architecture and independent genomic regions were associated with each trait. Single nucleotide polymorphisms on chromosomes Pv06, Pv07, Pv08, and Pv11 were associated with the number of egg masses, and SNPs on Pv01, Pv02, Pv05, and Pv10 were associated with root-galling. A total of 216 candidate genes were identified, including 14 resistance gene analogs and five differentially expressed in a previous RNA sequencing analysis. Histochemical analysis indicated that reactive oxygen species might play a role in the resistance response. Our findings open new perspectives to improve selection efficiency for RKN resistance, and the candidate genes are valuable targets for functional investigation and gene editing approaches.