Advances in contezolid: novel oxazolidinone antibacterial in Gram-positive treatment.

PURPOSE: The principal objective of this project was to review and thoroughly examine the chemical characteristics, pharmacological activity, and quantification methods associated with contezolid. METHODS: The article was based on published and ongoing preclinical and clinical studies on the application of contezolid. These studies included experiments on the physicochemical properties of contezolid, in vitro antimicrobial research, in vivo antimicrobial research, and clinical trials in various phases. There were no date restrictions on these studies. RESULTS: In June 2021, contezolid was approved for treating complicated skin and soft tissue infections. The structural modification of contezolid has resulted in better efficacy compared to linezolid. It inhibits bacterial growth by preventing the production of the functional 70S initiation complex required to translate bacterial proteins. The current evidence has indicated a substantial decline in myelosuppression and monoamine oxidase inhibition without impairing its antibacterial properties. Contezolid was found to have a more significant safety profile and to be metabolised by flavin monooxygenase 5, reducing the risk of harmful effects due to drug-drug interactions. Adjusting doses is unnecessary for patients with mild to moderate renal or hepatic insufficiency. CONCLUSION: As an oral oxazolidinone antimicrobial agent, contezolid is effective against multi-drug resistant Gram-positive bacteria. The introduction of contezolid provided a new clinical option.

[Effects of Long-term Tillage on Soil Bacterial Community Structure and Physicochemical Properties of Dryland Wheat Fields in Northern China].

To explore the effects of long-term tillage on bacterial community structure in different soil layers of dryland wheat fields and its relationship with soil physicochemical properties, a long-term field experiment was conducted from 2016 to 2021 in Wenxi Experimental Demonstration Base of Shanxi Agricultural University, Shanxi Province. We studied the effects of no-tillage (NT), subsoiling-tillage (ST), and deep plowing (DP) on soil physicochemical properties; α and ß diversity of the bacterial community; and dominant and different species of phyla and genera in different soil layers. Additionally, PICRUSt2 was used to predict the metabolic function of soil bacterial community. The results revealed that subsoiling-tillage and deep plowing significantly increased the soil water content in the 20-40 cm soil layer and significantly decreased the soil organic carbon content in the 0-20 cm soil layer compared with that under no-tillage for five consecutive years. Compared with that under deep plowing, subsoiling-tillage significantly increased soil water content, soil organic carbon content, dissolved organic carbon content, and dissolved organic nitrogen content in the 0-20 cm soil layer. Compared with that under no-tillage, subsoiling-tillage and deep plowing increased the α diversity of the soil bacterial community in the 0-40 cm soil layer, and subsoiling-tillage was higher than deep plowing. Compared with that under no-tillage, subsoiling-tillage and deep plowing significantly increased the relative abundances of Acidobacteria and Nitrospirae in the 0-20 cm soil layer and Acidobacteria, Chloroflexi, Gemmatimonadetes, Rokubacteria, GAL15, and Nitrospirae in the 20-40 cm soil layer. Compared with that under no-tillage, subsoiling-tillage and deep plowing significantly increased the relative abundance of Nitrospira in the 0-20 cm soil layer and Rubrobacter and Streptomyces in the 20-40 cm soil layer. Compared with that under deep plowing, subsoiling-tillage significantly increased the relative abundance of Acidobacteria and Gemmatimonadetes in the 0-40 cm soil layer. Redundancy analysis demonstrated that the contents of soil organic carbon, dissolved organic carbon, and dissolved organic nitrogen in the 0-20 cm soil layer exerted positive effects on Actinobacteria and Blastococcus, and the soil water content in the 0-40 cm soil layer exerted positive effects on Acidobacteria, Chloroflexi, and Gemmatimonadetes under subsoiling-tillage. The results of PICRUSt2 prediction showed that subsoiling-tillage and deep plowing significantly increased the relative abundance of amino acid metabolism and the metabolism of cofactors and vitamins but decreased the relative abundance of lipid metabolism of bacterial communities in the 20-40 cm soil layer compared with that under no-tillage. Compared with that under deep plowing, subsoiling-tillage significantly increased the relative abundances of amino acid metabolism in the 0-40 cm soil layer and other amino acid metabolism in the 0-20 cm soil layer. In conclusion, subsoiling-tillage or deep plowing could increase the soil water content, α diversity of the soil bacterial community, and their metabolic capacity in the dryland wheat fields during the summer fallow period. The relative abundance of Acidobacteria and Gemmatimonadetes and the ability of amino acid metabolism of the bacterial community were increased by subsoiling-tillage, and thus the contents of soil dissolved organic carbon and dissolved nitrogen can be increased.

Progress on genomics and locus of important agronomic traits in Chenopodium quinoa.

Quinoa (Chenopodium quinoa, Willd.) as a new health food in the 20th century, its comprehensive nutritional composition, stress resistance and other characteristics have been paid much of attention, and enjoys the reputation of "nutritional gold", "vegetarian king" and "food in the future" in the world. In recent years, with the rapid development of genomics and high-throughput sequencing technology, the high-quality whole genome sequence of quinoa has been completed, and the omics analysis and functional research of a series of key genes have been gradually carried out. In this review, we summarize the research progress in quinoa genomics, gene family analysis of important transcription factors, genetic map construction, QTL mapping of important traits, and genes for important agronomic and yield traits. Moreover, according to the current status of quinoa breeding, this paper also put forward five key problems in quinoa breeding, and pointed out four important directions of genetic improvement and breeding of quinoa in the future, so as to provide reference for the realization of directional genetic improvement of quinoa in the future.

Effects of sowing modes on soil water dynamics and grain protein formation in dryland wheat.

In order to clarify the mechanisms underlying dryland wheat quality improvement through sowing technology, the effects of wide space sowing (WSS), furrow sowing (FS), and drill sowing (DS) on soil moisture dynamics and grain protein formation in dryland wheat field were studied in Wenxi, Shanxi Province in 2017-2018 (normal year) and 2018-2019 (dry year). The results showed that compared with the DS, FS significantly increased soil water storage in the 0-200 cm depth at anthesis stage (8.2%-18.7%), and increased the soil water storage in 0-60 cm layer during the two years, 60-120 cm layer in normal year, and 120-200 cm layer in dry year. WSS significantly increased soil water storage in 0-200 cm layer at anthesis stage in normal year (5.2%). Compared with DS, FS significantly increased water consumption of anthesis to mature stage, while WSS significantly increased water consumption from sowing to anthesis stage in dry year. Compared with DS, the glutamine synthetase (GS) activity of grains at 15-35 days after anthesis was significantly increased by FS and WSS, while the glutamate synthase (GOGAT) activity of flag leaves and grains at 5-35 days after anthesis was significantly increased by FS in the dry year. Compared with DS, FS significantly increased grain yield by 20.4%-44.8%, grain protein yield by 25%-49%, and increased grain albumin and globulin contents. WSS significantly increased grain yield by 9%-40%, and increased grain gliadin content. Under different sowing modes of dryland wheat, GS and GOGAT activities in flag leaves and grains after anthesis were significantly correlated with water consumption at anthesis to maturity stage. There were significant correlations between GS and GOGAT activities in flag leaves and grains and 0-120 cm soil layers in the normal water year, and between GS and GOGAT activities and 0-60 cm, 120-200 cm soil layers in the dry year. The contents of grain albumin, globulin and total protein were significantly correlated with soil water storage in 0-60 cm layer at anthesis stage, as well as soil water storage in 120-200 cm layer at anthesis stage in the dry year. In conclusion, FS was beneficial to increasing soil water storage at anthesis and water consumption after anthesis of dryland wheat, improving GS and GOGAT activities of flag leaf and grain, with positive consequences on yield and grain protein content. In addition, deep water storage was more conducive to quality improvement in the late growth period in the dry year.

[Effects of deep ploughing during the fallow period and soil moisture-based furrow sowing on water and nitrogen utilization of dryland wheat.] / 休闲期深翻和探墒沟播对旱地小麦水氮资源利用的影响.

To understand the effects of deep ploughing during the fallow period and soil moisture-based furrow sowing on the utilization of water and nitrogen in dryland wheat, a field experiment following split-plot design was carried out from 2016 to 2018 in Wenxi County of Shanxi Province, with deep ploughing during the fallow period and no-tillage as main plots and soil moisture-based furrow sowing and drilling sowing as sub-plots. The results showed that, compared with no-tillage treatment, deep ploughing during the fallow period significantly increased soil water storage efficiency (by 38.3%-42.2%), soil water consumption (by 9.2%-13.2%), and nitrogen accumulation in each growth period, which in turn increased the yield by 7.1%-12.0%, annual water use efficiency by 5.5%-14.0%, nitrogen fertilizer absorption efficiency by 4.4%-10.3%, and nitrogen fertilizer partial productivity by 7.1%-12.0%. Compared with the treatment of drilling sowing, the soil moisture-based furrow sowing increased the total water consumption during the growth period (by 2.0%-4.8%) and nitrogen accumulation in each growth period, increased the yield by 6.8%-12.4%, water use efficiency during the growth period by 4.5%-7.2%, nitrogen absorption efficiency by 4.4%-10.3%, nitrogen partial productivity by 6.9%-12.4%. In conclusion, deep ploughing during the fallow period and soil moisture-based furrow sowing in dryland wheat could promote the storage and utilization of natural precipitation, increase plant nitrogen accumulation, and facilitate high wheat yield.

Effects of phosphorus fertilizer on root characteristics, uptake and utilization of phosphorus and yield of dryland wheat with contrasting yearly rainfall pattern.

To understand the growth responses of dryland wheat to different application rates of phosphorus fertilizer in different rainfall years, we examined root characteristics, spike number, yield and phosphate utilization. Results would help improve phosphate fertilizer use in dryland wheat production. We carried out a field experiment at the research station of Shanxi Agricultural University from 2012 to 2016. We examined the effects of four application rates of phosphorus (0, 75, 150 and 225 kg·hm-2 on root growth, phosphate utilization and yield formation of dryland wheat in different years with contrasting rainfall pattern. Compared with the treatment without phosphorus fertilization, phosphate application increased root surface area at all growth stages and root weight density in the 0-80 cm soil layer at jointing, anthesis, and maturity stages. Phosphate application significantly increased soil water consumption from jointing to anthesis, and total soil water consumption in the growing season. Phosphate application enhanced the amount of pre-anthesis phosphate translocation and phosphate accumulation of grain. Spike number, yield and water use efficiency were increased with 75, 150 and 225 kg P·hm-2 by 9.2% to 22.5%, 11.8% to 30.0%, and 2.1% to 12.1%, respectively. In the dry years, the application rates of 150 and 225 kg P·hm-2 in comparison to 75 kg P·hm-2 significantly increased root weight density and root surface area at all stages, soil water consumption from sowing to jointing and from jointing to anthesis, and total water consumption in the growing season. In comparison to the rate of 75 kg P·hm-2, 150 and 225 kg P·hm-2 increased soil water consumption from sowing to jointing by 7.3-8.7 mm, soil water consumption from jointing to anthesis by 15.6-18.1 mm, and total water consumption by 15.6-18.1 mm. Significant increase in the pre-anthesis phosphate translocation and phosphate accumulation in grain was higher under 150 and 225 kg P·hm-2 than that under 75 kg P·hm-2 in dry years. Furthermore, the two rates (150 and 225 kg P·hm-2) in dry years increased spike number by 9.3%-10.7% and yield by 11.9%-14.6%. The application rate of 150 kg P·hm-2 significantly improved phosphorus use efficiency by 20%-82% in comparison to other rates. In normal years, the rates of 150 and 225 kg P·hm-2 increased root surface area, root weight density at both anthesis and maturity compared with 75 kg P·hm-2. Soil water consumption from anthesis to maturity and total soil water consumption in the growing season were also increased by 1.2-15.0 and 3.8-23.1 mm, respectively. In addition, phosphorus accumulation in post-anthesis and phosphate accumulation in grain were increased in both 150 and 225 kg P·hm-2, which increased spike number by 1.4%-9.6% and yield by 3.5%-10.4%. The effects of phosphate application at the rate of 150 kg P·hm-2 were significantly different from 75 and 225 kg P·hm-2. In conclusion, phosphorus fertilizer application enhanced uptake of water and phosphate in dryland wheat at early and middle growth stages in dry years and at the late growth stage in normal years. Phosphorus application increased wheat yield mainly due to the increases of spike number. The application of 150 kg P·hm-2 is the best choice for high water and phosphorus fertilizer use efficiency and high yield in both dry and normal years.

Soil physical properties response to tillage practices during summer fallow of dryland winter wheat field on the Loess Plateau.

Soil physical properties are a greatly important part of the soil and indicator of soil quality, which can directly affect soil nutrient turnover and crop yields in dryland. This study was carried out with three tillage practices during the summer fallow season since 2011, including no tillage (NT), plow tillage (PT), and subsoiling (ST) in dryland winter wheat fields of the Loess Plateau. Results showed that soil tillage during the summer fallow had a small effect on soil bulk density (ρ b) in the 0-50-cm soil profile before sowing and after harvesting of winter wheat. Soil ρ b under NT at a depth of 20-30 cm was significantly greater than those under PT in both seasons. Both soil gravimetric water content (θ g) and volumetric moisture content (θ v) after harvesting increased by 28.8-78.6% and 37.5-87.3%, respectively, compared with those before sowing. Adoption of PT significantly increased soil θ g and θ v in the entire 0-50-cm profile before sowing compared with NT and ST (P < 0.05). In addition, there was a small effect on soil porosity (e.g., total porosity, air-filled porosity, and capillary porosity) in the profile of 0-50 cm both before sowing and after harvesting. Overall, short-term tillage during summer fallow mainly affected soil water content in the 0-50-cm soil profile, and it had a slight effect on other physical soil properties.

[Relationships of water conservation through mulching in fallow period with wheat nitrogen transportation and crop yield in dryland].

Field experiments were carried out to study the effects of different mulching times (30 or 60 d after previous wheat harvest) and mulching methods (whole-mulching, half-mulching and no- mulching) on wheat plant N absorption and assimilation after deep plowing in fallow period on dryland. The results showed that mulching improved water storage efficiency in fallow period and soil water storage of 0-300 cm at sowing stage significantly. Mulching in fallow period, especially the whole-mulching, increased the N accumulation amount of each growth stage, N translocation amount and rate before anthesis (NABA) , and N accumulation amount after anthesis (NAAA) and grain N. Yield, N uptake efficiency (NUPE), N partial factor productivity (NPFP) and N harvest index (NHI) were all significantly increased under mulching, and whole-mulching had better effects. Mulching at 30 d after previous wheat harvest significantly increased the soil water storage of 0-300 cm at sowing stage and the water storage efficiency in fallow period compared with mulching at 60 d after previous wheat harvest. Compared with mulching at 60 d after previous wheat harvest, mulching at 30 d increased the N accumulation amount of each growth stage, the N accumulation amount in leaf and shoot at maturity, the NABA of stem + sheath, leaf and shoot and the yield. The soil water at the depth of 0-300 cm at sowing stage was positively correlated to N mobilization amount before anthesis and N accumulation amount after anthesis. The N mobilization amount of stem + sheath had a remarkable direct effect on its yield with the direct path coefficient of 0.619. In summary, mulching in fallow period increased the soil moisture at sowing stage to promote N absorption and utilization, increase yield and improve quality, and whole-mulching in advance had better effects.

[Effects of tillage in fallow period on soil water and nitrogen absorption and translocation by wheat plant].

Field test was carried out to study the effect of tillage in fallow period on soil water before sowing and growth stages, and nitrogen (N) absorption, translocation by wheat plant. The current data showed that tillage in fallow period improved the soil water at the depth of 0-300 cm before sowing and growth stages, especially in dry years. Such tillage significantly improved N accumulation in leaf, stem and sheath (SS) at anthesis, grain N accumulation at maturity, N mobilization in SS and the contribution of mobilized N to grain N, amount of mobilized N in leaf, level of N accumulation before anthesis, N transportation from vegetative organs to grains after anthesis, and nitrogen accumulation after anthesis, which in turn enhanced the efficiency of N uptake. Deep tillage at 45 days after harvest had the best effect. Significant correlations were detected between soil water and N accumulation before anthesis as well as N translation from vegetative organs grains after anthesis, particularly in dry years, while the correlation between soil water from sowing to an thesis and nitrogen accumulation amount after anthesis was significant in wet years, but not in dry years. Tillage in fallow period especially deep tillage after raining could benefit soil water preservation, as well as N absorption and translocation by plant.

[Expression and activity determination of TNFR domain of osteoprotegerin in E.coli and corresponding antibody preparation].

Osteoprotegerin (OPG) plays an important role in the regulation of bone resorption and remodeling. The TNFR domain of OPG, which is involved in the inhibition of formation and activity of osteoclasts, was amplified by PCR and inserted into multiple cloning site of PET-28a. The recombinant plasmid was transferred into E.coli BL21 to express recombinant protein. It was found that expressed product existed in the form of inclusion body. The inclusion body was solubilized, renatured and purified by affinity chromatography. Polyclonal antibodies with high specificity were obtained from the serum of rabbit immunized with purified recombinant protein. Mice were used to determine the hypocalcemic effect of the recombinant protein. Results showed that the recombinant protein expressed in E.coli had the proper bioactivity.

Expression, purification and sublocalization of SARS-CoV nucleocapsid protein in insect cells.

The causative agent of severe acute respiratory syndrome (SARS) is a previously unidentified coronavirus, SARS-CoV. The nucleocapsid (N) protein of SARS-CoV is a major viral protein recognized by acute and early convalescent sera from SARS patients. To facilitate the studies on the function and structure of the N protein, this report describe the expression and purification of recombinant SARS-CoV N protein using the baculovirus expression system. Recombinant hexa-histidine-tagged N protein with a molecular mass of 47 kD was produced in insect cells. Recombinant N protein was purified to near homogeneity by Ni2+-NTA affinity chromatography. In addition, we examined the subcellular localization of the N protein by confocal microscopy in Trichoplusia ni BT1 Tn 5B1-4 cells infected with recombinant baculovirus. The N protein was found localized in the cytoplasm as well as in the nucleolus. The purified recombinant N protein can be used in further functional study of SARS-CoV.