RESUMEN
In this study, we investigated in an androgenized rat model the involvement of autophagy and mitochondrial dynamics in granulosa cells in the pathogenesis of polycystic ovarian syndrome (PCOS) and its modulation by exogenous gonadotropin (eCG). We found 5α-dihydrotestosterone (DHT) treatment reduces ovarian length and weight with predominantly late antral and/or preovulatory stage follicles and no corpora lutea. DHT increased the population of large lysosomes (>50 micron) and macroautophagy, an event associated with granulosa cell apoptosis. Increased granulosa cell Dynamin Related Protein 1 (Drp1) content in the DHT group was accompanied by increased circular and constricted, but reduced rod-shaped, mitochondria. eCG eliminated all atypical follicles and increased the number of late antral and preovulatory follicles with less granulosa cell apoptosis. eCG-treated rats had a higher proportion of connected mitochondria, and in combination with DHT had a lower proportion of circular and constricted mitochondria than rats treated with DHT alone, suggesting that eCG induces mitochondrial fusion and attenuates fission in granulosa cells. In summary, we observed that DHT-induced up-regulation of Drp1 is associated with excessive mitochondrial fission, macroautophagy and apoptosis in granulosa cells at the antral stage of development in an androgenized rat model for PCOS, a response partially attenuated by exogenous gonadotropin.
Asunto(s)
Andrógenos/farmacología , Dihidrotestosterona/farmacología , Dinaminas/metabolismo , Células de la Granulosa/metabolismo , Mitocondrias/fisiología , Síndrome del Ovario Poliquístico/patología , Animales , Apoptosis/fisiología , Autofagia/fisiología , Modelos Animales de Enfermedad , Femenino , Gonadotropinas/farmacología , Mitocondrias/efectos de los fármacos , Dinámicas Mitocondriales/fisiología , Ratas , Ratas Sprague-DawleyRESUMEN
Germ plasm, a cytoplasmic factor of germline cell differentiation, is suggested to be a perspective tool for in vitro meiotic differentiation. To discriminate between the: (1) germ plasm-related structures (GPRS) involved in meiosis triggering; and (2) GPRS involved in the germ plasm storage phase, we investigated gametogenesis in the marine medaka Oryzias melastigma. The GPRS of the mitosis-to-meiosis period are similar in males and females. In both sexes, five events typically occur: (1) turning of the primary Vasa-positive germ plasm granules into the Vasa-positive intermitochondrial cement (IMC); (2) aggregation of some mitochondria by IMC followed by arising of mitochondrial clusters; (3) intramitochondrial localization of IMC-originated Vasa; followed by (4) mitochondrial cluster degradation; and (5) intranuclear localization of Vasa followed by this protein entering the nuclei (gonial cells) and synaptonemal complexes (zygotene-pachytene meiotic cells). In post-zygotene/pachytene gametogenesis, the GPRS are sex specific; the Vasa-positive chromatoid bodies are found during spermatogenesis, but oogenesis is characterized by secondary arising of Vasa-positive germ plasm granules followed by secondary formation and degradation of mitochondrial clusters. A complex type of germ plasm generation, 'the follicle cell assigned germ plasm formation', was found in late oogenesis. The mechanisms discovered are recommended to be taken into account for possible reconstruction of those under in vitro conditions.
Asunto(s)
Gránulos Citoplasmáticos/fisiología , ARN Helicasas DEAD-box/metabolismo , Células Germinativas/citología , Oocitos/citología , Oogénesis , Oryzias/crecimiento & desarrollo , Espermatocitos/citología , Espermatogénesis , Animales , Núcleo Celular , Gránulos Citoplasmáticos/ultraestructura , Femenino , Proteínas de Peces/metabolismo , Células Germinativas/metabolismo , Células Germinativas/ultraestructura , Masculino , Oocitos/metabolismo , Espermatocitos/metabolismoRESUMEN
Current peroxisome proliferator-activated receptor (PPAR)-targeted drugs, such as the PPARγ-directed diabetes drug rosiglitazone, are associated with undesirable side effects due to robust agonist activity in non-target tissues. To find new PPAR ligands with fewer toxic effects, we generated transgenic zebrafish that can be screened in high throughput for new tissue-selective PPAR partial agonists. A structural analog of coenzyme Q10 (idebenone) that elicits spatially restricted partial agonist activity for both PPARα and PPARγ was identified. Coenzyme Q10 was also found to bind and activate both PPARs in a similar fashion, suggesting an endogenous role in relaying the states of mitochondria, peroxisomes and cellular redox to the two receptors. Testing idebenone in a mouse model of type 2 diabetes revealed the ability to reverse fatty liver development. These findings indicate new mechanisms of action for both PPARα and PPARγ, and new potential treatment options for nonalcoholic fatty liver disease (NAFLD) and steatosis.This article has an associated First Person interview with the first author of the paper.
Asunto(s)
Enfermedad del Hígado Graso no Alcohólico/tratamiento farmacológico , PPAR alfa/metabolismo , PPAR gamma/metabolismo , Ubiquinona/análogos & derivados , Células 3T3-L1 , Animales , Animales Modificados Genéticamente , Benzoquinonas/química , Benzoquinonas/farmacología , Benzoquinonas/uso terapéutico , Evaluación Preclínica de Medicamentos , Células HEK293 , Humanos , Ligandos , Metabolismo de los Lípidos/efectos de los fármacos , Hígado/efectos de los fármacos , Hígado/metabolismo , Hígado/patología , Masculino , Ratones , Ratones Endogámicos C57BL , PPAR alfa/agonistas , PPAR gamma/agonistas , Ubiquinona/química , Ubiquinona/farmacología , Ubiquinona/uso terapéutico , Pez CebraRESUMEN
To highlight the ultrastructural features of transformation occurring with germplasm-related structures (GPRS), the spermatogenic cells of Xenopus laevis were studied by transmission electron microscopy and quantitative analysis. It was determined that in spermatogonia and spermatocytes, the compact germinal granules underwent fragmentation into particles comparable with inter-mitochondrial cement (IMC). Fragments of IMC agglutinated some cell mitochondria and resulted in the creation of mitochondrial clusters. Clustered mitochondria responded with loss of their membranes that occurred by the twisting of membranous protrusions around themselves until multi-layered membranes were formed. The mitochondrial affinity of multi-layered membranes was proven by an immunopositive test for mitochondrial dihydrolipoamide acetyltransferase. As a consequence of mitochondrial membrane twisting, the naked mitochondrial cores appeared and presumably underwent dispersion, which is the terminal stage of GPRS transformation. As no GPRS were observed in spermatids and sperm, it was assumed that these structures are functionally assigned to early stages of meiotic differentiation.
Asunto(s)
Testículo/citología , Animales , Diferenciación Celular , Masculino , Meiosis , Microscopía Inmunoelectrónica , Mitocondrias/ultraestructura , Espermátides/ultraestructura , Espermatocitos/ultraestructura , Testículo/ultraestructura , Xenopus laevisRESUMEN
Mouse Vasa homologue (MVH) antibodies were applied to mouse Graafian oocytes to clarify if mitochondrion-originated germinal body-like structures, described previously by conventional electron microscopy, were associated with the germ plasm. It was found that both the mitochondrion-like structures with cristae and the germinal body-like structures that lacked any signs of cristae were labelled specifically by the anti-MVH antibody. Moreover, some granules were MVH-positive ultrastructural hybrids of the mitochondria and germinal body-like structures, the presence of which clearly supported the idea of a mitochondrial origin for the germinal body-like structures. This finding is the first evidence that mitochondrion-originated germinal body-like granules represent mouse germ plasm.
Asunto(s)
ARN Helicasas DEAD-box/metabolismo , Oocitos/metabolismo , Oocitos/ultraestructura , Orgánulos/metabolismo , Animales , Western Blotting , ARN Helicasas DEAD-box/inmunología , Femenino , Masculino , Ratones , Microscopía Inmunoelectrónica , Mitocondrias/metabolismo , Mitocondrias/ultraestructura , Oocitos/citología , Orgánulos/ultraestructuraRESUMEN
The germ plasm-related structures (GPRS) and the transformation that occurs to them during the spermatogenesis of the sea urchin Anthocidaris crassispina were studied by electron microscopy and morphometry. The GPRS were observed in spermatogonia and spermatocytes, but not in spermatids and sperm, which suggests an important role for these structures during the onset of meiosis. It was proposed that the germinal granules are fragmented into the compact electron-dense nuage, and fragments of the latter penetrate into the periphery of the compact electron-lucent nuage. The process of nuage integration is completed with the formation of the combined nuage, which aggregates some mitochondria into clusters. Once formed, the mitochondrial clusters undergo dissemination and assume the appearance of the dispersed nuage with mitochondrial derivatives, which in turn develops into the scattered nuage. The scattered nuage, which presumably presents the composite mixture saturated with mitochondrial matrix, terminates the GPRS transformation.
Asunto(s)
Anthocidaris/fisiología , Espermatogénesis/fisiología , Espermatozoides/ultraestructura , Animales , Masculino , Mitocondrias/ultraestructura , Espermátides/ultraestructura , Espermatocitos/ultraestructura , Espermatogonias/ultraestructuraRESUMEN
The alterations involved in oocyte atresia of the starfish Pisaster ochraceus were investigated using both light and electron microscopy. It was shown that atresia may be defined by three patterns of cell destruction. Initially, the small electron-lucent vesicles produced by the Golgi complex underwent amalgamation into groups. This was followed by loss of vesicle membranes and consequent formation of transparent necrotic zones in the cytoplasm. The second pattern, ultrastructurally comparable with autophagic cell death, was marked by apparent amalgamation of the morphologically similar electron-lucent vesicles into growing vacuoles, giving rise to a multibranched autophagic vacuole. This vacuole engulfed the cytosol granules and ultimately came to occupy the entire space within the oocyte. In addition, the cytosol insulation inside of the 'apoptotic body-like spheres' was regularly observed. Thus, it is supposed that oocyte destruction may occur by a complex mechanism that includes elements of necrosis, autophagic cell death and apoptosis.
Asunto(s)
Estrellas de Mar/ultraestructura , Animales , Muerte Celular/fisiología , Femenino , Oocitos/metabolismo , Oocitos/ultraestructura , Estrellas de Mar/metabolismoRESUMEN
To find more morphological characteristics useful for discussion on aralian or non-aralian Panax affinity, pollen morphological diversity was comparatively analysed in P. ginseng, Aralia elata and Oplopanax elatus collected during their pollination periods. In the anthers of both the buds and open flowers, the pollen average diameter varied between some species-specific maximum and minimal measurement. However, the larger pollen grains were typically found in the buds whereas the smaller pollen prevailed in the open flowers, testifying to the pollen size diminution during anther maturation. Based on this finding, the subsequent examination of pollen according to size decrease was put into operation as a method of pollen modification for the study. The structural mechanisms of pollen metamorphosis were identified as not being species specific but rather universal. These mechanisms are suggested to be the shrinkage of the pollen vegetative cytoplasm, the intine enlargement, the deepening of three colporate apertures provided by exine sunken into enlarged intine areas, the aperture accretion as well as the transformation of the exine from thick/sculptured into thin/less sculptured. During 'size-reducing metamorphosis', the pollen grains changed dramatically, going through a species-specific set of intermediate morphs to the final species-specific morphotype. In P. ginseng this morphotype is round (diameter is about 16 microm), in A. elata it is round with a single projection (diameter is about 15 microm) and in O. elatus it is ovoid with a single projection (average diameter is about 18 microm). In addition, every species is peculiar in having the unique vegetative cytoplasm inclusions and individual construction of the largest pollen exine. From a phylogenetic perspective, these findings presumably add support to the option of equal remoteness of P. ginseng from A. elata and O. elatus. The characteristics found seem to be suitable for examination of Panax affinity, by the subsequent study of more Araliaceae representatives.
Asunto(s)
Aralia/ultraestructura , Oplopanax/ultraestructura , Panax/ultraestructura , Polen/crecimiento & desarrollo , Polen/ultraestructura , Aralia/clasificación , Oplopanax/clasificación , Panax/clasificación , Especificidad de la EspecieRESUMEN
Phagocytic resorption during spermatogenesis was studied in the sea urchin Anthocidaris crassispina. Nutritive phagocytes in gonad absorbed both waste sperm cells and residual bodies discarded from maturing spermatids, and these materials were subsequently compartmented in heterophagosomes. Based on 180 heterophagosomes examined by transmission electron microscopy, over 99% of heterophagosomes contained either residual bodies or sperm cells only. Simultaneous resorption of sperm cells and residual bodies in a heterophagosome was uncommon, with only approximately 0.56% occurrence, suggesting that heterophagosomes have a selective resorption ability in nutritive phagocytes.
Asunto(s)
Fagocitos/citología , Erizos de Mar/embriología , Animales , Embrión no Mamífero , Masculino , Fagocitos/ultraestructura , Espermatozoides/fisiología , Espermatozoides/ultraestructuraRESUMEN
Adult sea urchins, Anthocidaris crassispina, were exposed to 0.1 and 10 mgL(-1) phenol for 4 weeks. Abnormal sperm development was clearly evident in phenol-treated sea urchins, although no mortality was found throughout the exposure period. Occurrences of multinucleate sperm cells (including spermatocytes to spermatozoa) showed a significant increase from 0.07% in the control to 10.7% and 43.3% in the 0.1- and 10-mgL(-1) treatments, respectively (P<0.01). Likewise, sperm with electron dense, dark tails increased significantly from 8% in the control to 36.6% and 43.4% in the 0.1- and 10-mgL(-1) phenol-treated sea urchins, respectively (P<0.01). In addition, disruption of cytoplasmic membranous structures such as loss of mitochondrial cristae and distortion of mitochondrial membranes and nucleus envelope were commonly found in phenol-treated spermatogonia and spermatocytes. Previous studies have clearly demonstrated motility impairment and a concomitant reduction of fertilization capability in sea urchin sperm with dark tails and/or distorted mitochondria. Our current findings therefore suggest that chronic exposure to phenol at 0.1 mgL(-1) could lower the quality of sperm and reproductive success in sea urchins, which may threaten the survival of these ecologically important species. The observed impairment of spermatogenesis by phenol might also occur in other invertebrate species.