Estimation of genetic parameters for maintenance energy requirements and residual feed intake in Nellore cattle.

We estimated heritabilities and genetic and phenotypic correlation estimates for maintenance energy requirements (NEmR), residual feed intake (RFI), growth, carcass and reproductive indicator traits, using data from 41 feed efficiency trials in Brazil, comprising 4381 males and females. Continuous traits were analysed using a linear animal model and threshold traits were analysed using a threshold animal model. The heritability estimates were low for RFI (0.190) and NEmR (0.193); other heritabilities were mainly moderate (growth and carcass traits) or high (sexual precocity traits). The genetic correlation of RFI with NEmR was high (0.701). The genetic correlations of NEmR were low with carcass and reproductive traits, and moderate with growth traits. Thus, selection to improve weaning weight and female sexual precocity indicator traits would not affect maintenance energy requirement. Genetic selection to reduce maintenance energy requirements is feasible and would also reduce DMI and RFI. Selection to improve RFI can be used to identify animals with lower maintenance energy requirements. Long-term selection to reduce RFI and NEmR would have favourable effects on yearling weight, carcass muscle indicator traits and female sexual precocity. Genetic (co)variance component estimates for NEmR, in conjunction with economic values of selection criteria, may be used to develop novel approaches for genetic selection to improve efficiency of beef production.

Maternal exposure to glyphosate-based herbicide causes vascular dysfunction in offspring female rats.

This study analyzed how glyphosate exposure in the gestational period affects vascular function in their female offspring and whether oxidative stress is involved in this effect. To this, pregnant Wistar rats were exposed through drinking water to 0.2% of a glyphosate commercial formulation, and we analyzed the response to acetylcholine and phenylephrine in the aorta from offspring of Glyphosate-based herbicide (O-GBH) and controls (O-CON) rats at six months of age. Relaxation to acetylcholine was reduced in O-GBH than in O-CON. Acute Indomethacin and Apocynin increased relaxation to acetylcholine in O-GBH. The aorta from O-GBH was hyperactive to phenylephrine; the preincubation with N-nitro-L-arginine methyl ester (L-NAME) increased contraction to phenylephrine more in O-CON than O-GBH. TEMPOL similarly reduced phenylephrine response, and L-NAME prevented this effect. The TBARS and GSH levels were increased in O-GBH than in O-CON. Results reinforce the concept that oxidative stress during the perinatal period contributes to the development of vascular changes in adulthood. Results also reveal that oxidative stress parameters altered, and the current levels considered safe for exposure to Glyphosate deserve further investigation, especially in the female gender.

Wound Healing Effect of Lippia sidoides and Myracrodruon urundeuva Nanogel.

Wound healing is a natural regenerative response to tissue injury and the conventional treatments consists of the use wound dressings with local administration of medicines, but, in some cases, are only partially effective and limited by toxicity or ineffective anti-microbial protection. Medicinal plants such as Lippia sidoides and Myracrodruon urundeuva have shown interesting pharmacological activities, allied to this, the association of these medicinal plants and nanotechnology, could mean an advantage in relation to classical approach. This study investigated the effect of a nanogel loaded with Lippia sidoides essential oil and Myracrodruon urundeuva extract (NAA) in an excisional wound healing model in rats. Animals were anesthetized and skin wounds were made using a metal punch. The groups were treated with vehicle, NAA or collagenase gel, for 7, 14 or 21 days and then sacrificed for tissue analysis. NAA did not show acute dermal irritation, further significantly reduced (p<0.05) the final wound area, accelerated the wound contraction and organization of collagen in the group treated for 14 days. The data presented here demonstrate the therapeutic potential for the use of nanotechnology associated with medicinal plants and provides evidence that corroborate with the use of L. sidoides and M. urundeuva as healing medicinal plants.

Vulnerabilidades de pescadores de comunidades ribeirinhas às Infecções Sexualmente Transmissíveis / Vulnerabilidad de los pescadores de las comunidades costeras a las infecciones de transmisión sexual / Vulnerability of fishermen from coastal communities to sexually transmitted infections

RESUMO Introdução: as Infecções Sexualmente Transmissíveis, especificamente a Síndrome da Imunodeficiência Adquirida são patologias que representam grandes desafios para a saúde no contexto mundial, merecendo destaque devido ao seu alto potencial de disseminação. No universo masculino, verifica-se que vem se intensificando o aumento da incidência dessas patologias, sobretudo nos grupos representados pelos adolescentes e jovens. Objetivo: analisar as vulnerabilidades de pescadores de comunidades ribeirinhas às infecções sexualmente transmissíveis. Métodos: trata-se de uma pesquisa exploratória e descritiva de abordagem qualitativa, realizada nas comunidades ribeirinhas do interior piauiense, Brasil. A amostra foi composta por 18 homens que vivem nessas comunidades. Resultados: os resultados foram divididos em duas categorias, a saber: Vulnerabilidades de pescadores relacionadas as infecções sexualmente transmissíveis e práticas preventivas; Políticas públicas de saúde voltadas para a saúde sexual dos homens. Percebeu-se que há uma grande deficiência de conhecimento dos pescadores sobre essas infecções, devido ao baixo grau de escolaridade e a ausência de ações educativas em saúde por parte da Estratégia de Saúde da Família, o que os torna muito vulneráveis às Infecções Sexualmente Transmissíveis. Conclusão: há uma necessidade de realizar ações educativas em saúde para os pescadores, por parte da Estratégia Saúde da Família, visto que as políticas de saúde para os homens contemplam ações para as populações mais vulneráveis(AU)

RESUMEN Introducción: las infecciones de transmisión sexual son enfermedades que constituyen importantes desafíos para la salud en el contexto global, y el síndrome de inmunodeficiencia adquirida merece una mención especial debido a su alto potencial de propagación. En el universo masculino, parece que se está intensificando la creciente incidencia de estas enfermedades, especialmente en los grupos representados por adolescentes y jóvenes. Objetivo: analizar las vulnerabilidades de pescadores de comunidades ribereñas con las infecciones de transmisión sexual. Métodos: estudio con enfoque cualitativo, exploratorio y descriptivo, llevado a cabo en las comunidades ribereñas de Piauí interior. La muestra consistió en 18 hombres que viven en estas comunidades. Resultados: se dividieron en dos categorías, a saber: los pescadores de vulnerabilidad relacionados con las prácticas de prevención de infecciones y las políticas públicas de salud centrados en la salud sexual de los hombres de transmisión sexual. Se observó que existe deficiencia de los conocimientos de los pescadores sobre estas infecciones, debido al bajo nivel de educación y la falta de las acciones en salud en la Estrategia Salud de la Familia, que los hace muy vulnerables a las infecciones de transmisión sexual. Conclusión: se necesitan actividades de educación en la salud de los pescadores, mediante la estrategia de salud de la familia, como las políticas de salud para los hombres que incluyen acciones para las poblaciones más vulnerables(AU)

ABSTRACT Introduction: Sexually transmitted infections, specifically the acquired immunodeficiency syndrome are diseases that pose major challenges for health in the global context deserves special mention because of its high potential for spread. In the male universe, it appears that is intensifying the increasing incidence of these diseases, especially in the groups represented by adolescents and youth. Objective: Analyze the fishing vulnerabilities related to sexuality and sexually transmitted infections prevention practices. Methods: This is an exploratory and descriptive qualitative approach, carried out in the riverine communities of Piaui interior. The sample consisted of 18 men who live in these communities. Results: The results were divided into two categories, namely: Vulnerability fishermen related and prevention practices Sexually Transmitted Infections; health public policies focused on the sexual health of men. It was noticed that there is a big fishermen's knowledge deficiency on these infections, due to the low level of education and lack of educative health actions by the Family Health Strategy, which makes them very vulnerable to sexually transmitted infections. Conclusion: There is a need for educational activities in health for fishermen, by the Family Health Strategy, as health policies for men include actions for the most vulnerable populations(AU)

Anti-inflammatory and healing action of oral gel containing borneol monoterpene in chemotherapy-induced mucositis in rats ( Rattus norvegicus )

ABSTRACT The aim of this study was to investigate the effect of gels containing the monoterpene borneol in induced oral mucositis using an animal model. Gels were prepared with borneol at 1.2% and 2.4% (w/w). Oral mucositis was induced by administration of three doses of 5-fluorouracil (30 mg/kg, i.p.) and injury with acetic acid (50%, v/v) soaked in filter paper applied to right cheek mucosa for 60s. Four subgroups comprising 12 animals each were formed. Six animals from each group were sacrificed at days seven and fourteen after oral mucositis induction. Mucous samples were processed and stained with hematoxylin-eosin and Masson's Trichrome. The semiquantitative evaluation involved observation of inflammatory parameters. ImageJ® software was used in the quantitative evaluation. For statistical analyses, Two-way ANOVA, followed by Tukey's post-test (p <0.05), were employed. Borneol 2.4% gel proved effective in the treatment of oral mucositis with statistically significant differences between groups for angiogenesis control, inflammatory cell count reduction and percentage neoformed collagen increase. The confirmation of anti-inflammatory and healing action of borneol in oral mucositis in rats renders it a good marker for predicting this activity for plant extracts rich in this substance

Easysolution: correção ortodôntica com alinhadores estéticos / Easysolution: orthodontic correction with esthetic aligners ­ a case report

Os alinhadores podem ser uma opção de tratamento para correção de más-oclusões dentárias que envolvam, principalmente, os dentes anteriores. O sistema Easysolution é planejado com setup virtual e precisão nos movimentos dentários, alcançando melhor resultado. O objetivo deste trabalho foi apresentar a correção de um apinhamento suave na região anterior, com giroversões dentárias, além de discutir as vantagens e desvantagens desse sistema. O presente estudo confirma que é possível corrigir apinhamentos em dentes anteriores, e giroversões de incisivos de até 22°, com o sistema Easysolution.

The aligners can be a treatment option to correct dental malocclusions, mainly involving the anterior teeth. The Easysolution system is designed with virtual setup, with greater precision in tooth movement, achieving a better result. This case presents the correction of a slight crowding in the anterior region, and the giroversion of teeth 11, 21, 32 and 42, discussing the advantages and disadvantages of this system. This case report confirms that it is possible to correct crowding in anterior teeth and incisor rotations of up to 22°.

Primeira descrição da caracterização fenotípica e susceptibilidade in vitro a drogas de leveduras do gênero Cryptococcus spp isoladas de pacientes HIV positivos e negativos, Estado de Mato Grosso / First description of phenotypic profile and in vitro drug susceptibility of Cryptococcus spp yeast isolated from HIV-positive and HIV-negative patients in State of Mato Grosso

Foram avaliados 37 isolados de 10 pacientes HIV negativos e 26 positivos, em Mato Grosso. Exame direto, cultura e quimiotipagem de espécies foram realizados. Cetoconazol, itraconazol, voriconazol, fluconazol e anfotericina B foram avaliados. Foram identificadas 37 leveduras do gênero Cryptococcus spp sendo 26 de pacientes HIV- positivos (25 Cryptococcus neoformans e um Cryptococcus gattii) e 10 de HIV- negativos (cinco Cryptococcus neoformans e cinco Cryptococcus gattii). Considerando isolados clínicos (Cryptococcus neoformans) de HIV positivos observou-se resistência (8 por cento e 8,7 por cento) e susceptibilidade dose-dependência (20 por cento e 17,4 por cento) para fluconazol e itraconazol respectivamente. Para isolados de Cryptococcus neoformans oriundos de pacientes HIV negativos, observou-se susceptibilidade dose-dependência (40 por cento) ao fluconazol. Os isolados de Cryptococcus gattii provenientes de pacientes HIV- negativos mostraram-se susceptíveis a todos os antifúngicos, exceto um isolado de Cryptococcus gattii que foi susceptível dose-dependente ao fluconazol (20 por cento). O isolado proveniente do paciente HIV- positivo demonstrou resistência ao fluconazol (CIM > 256µg/mL) e itraconazol (CIM=3µg/mL).

Thirty-seven isolates from 10 HIV-negative and 26 HIV-positive patients in Mato Grosso were evaluated. Direct examination, culturing and chemotyping of species were performed. Ketoconazole, itraconazole, voriconazole, fluconazole and amphotericin B were evaluated. Thirty-seven yeasts of Cryptococcus spp were identified, of which 26 were from HIV-positive patients (25 Cryptococcus neoformans and one Cryptococcus gattii) and 10 from HIV-negative patients (five Cryptococcus neoformans and five Cryptococcus gattii). The Cryptococcus neoformans clinical isolates from HIV-positive patients showed resistance (8 percent and 8.7 percent) and dose-dependent susceptibility (20 percent and 17.4 percent) to fluconazole and itraconazole, respectively. Among the Cryptococcus neoformans isolates from HIV-negative patients, there was dose-dependent susceptibility (40 percent) to fluconazole. Cryptococcus gattii isolates from HIV-negative patients were shown to be susceptible to all antifungal agents, except for one isolate of Cryptococcus gattii that showed dose-dependent susceptibility to fluconazole (20 percent). The Cryptococcus gattii isolate from an HIV-positive patient showed resistance to fluconazole (MIC > 256 »g/ml) and itraconazole (MIC = 3 »g/ml).

Micoses sistêmicas: fatores associados ao óbito em pacientes com infecção pelo vírus da imunodeficiência humana, Cuiabá, Estado de Mato Grosso, 2005-2008 / Systemic mycosis: factors associated with death among patients infected with the human immunodeficiency virus, Cuiabá, State of Mato Grosso, Brazil, 2005-2008

A prevalência de micose sistêmica entre 1.300 pacientes portadores de HIV/Aids de Cuiabá, Mato Grosso foi de 4,6 por cento, no período de 2005-2008. As espécies de fungos isoladas foram o Cryptococcus neoformans (50 por cento), Cryptococcus gattii (1,6 por cento), Cryptococcus spp (6,6 por cento), Histoplasma capsulatum (38,3 por cento) e Paracoccidioides brasiliensis (3,3 por cento). Óbito foi registrado em 32 (53,3 por cento) pacientes, sendo a criptococose a principal causa. A contagem de linfócitos T CD4+ foi baixa e semelhante entre os pacientes que sobreviveram ou faleceram por micose sistêmica. O etilismo (OR:8,2; IC95 por cento: 1,4-62,1; p=0,005) e o nível médio de desidrogenase lática [758 (182) U/L vs 416 (268) U/L; p<0,001] foram as características independentemente associadas ao óbito dos pacientes do estudo. Os resultados mostram alta letalidade por micoses sistêmicas em pacientes portadores de HIV/Aids de Cuiabá e sugerem que características clínico-laboratoriais tais como o etilismo e a elevação precoce da desidrogenase lática podem ser fatores relacionados ao pior prognóstico nessas condições.

Between 2005 and 2008, the prevalence of systemic mycosis among 1,300 HIV/AIDS patients in Cuiabá, Mato Grosso, was 4.6 percent. The fungus species isolated were Cryptococcus neoformans in 50 percent, Cryptococcus gattii in 1.6 percent, Cryptococcus spp in 6.6 percent, Histoplasma capsulatum in 38.3 percent and Paracoccidioides brasiliensis in 3.3 percent. Death was recorded in the cases of 32 patients (53.3 percent), and cryptococcosis was the main cause. The CD4+ T lymphocyte count was low and similar among patients who survived or died due to systemic mycosis. The factors independently associated with the deaths of these patients were alcoholism (OR: 8.2; 95 percent CI: 1.4-62.1; p = 0005) and the mean level of lactate dehydrogenase [758 (182) U/l vs. 416 (268) U/l; p < 0001]. The findings showed that systemic mycosis was highly lethal among the patients with HIV/AIDS in Cuiabá and suggested that clinical-laboratory characteristics such as alcoholism and early elevation of lactate dehydrogenase may be factors relating to worse prognosis under these conditions.

O Ácido (8)17,12E,14-labdatrieno-18-óico (labdano302), diterpeno tipo labdano isolado de Xylopia langsdorffiana St. Hil. & Tul. (Annonaceae) relaxa a traquéia isolada de cobaia / The (8)17,12E,14-labdatrien-18-oic acid (labdane302), labdane-type diterpene isolated from Xylopia langsdorffiana St. Hil. & Tul. (Annonaceae) relaxes the guinea-pig trachea

A espécie Xylopia langsdorffiana St. Hil.. & Tul. é popularmente conhecida como "pimenteira-da-terra" no Sudeste do Brasil. A partir do fracionamento do extrato etanólico, obtido das cascas do caule desta espécie, foi isolado um diterpeno tipo labdano, identificado como sendo o ácido 8(17),12E,14-labdatrieno-18-óico, e que neste trabalho é codificado como labdano302. O labdano302 relaxou o tônus basal dos anéis de traquéia isolada de cobaia com um valor de CE50 de 6,7 ± 0,5 x 10-8 M. O diterpeno labdano302 relaxou de maneira dependente de concentração os anéis pré-contraídos com carbacol (10-6 M), tanto na presença (CE50 = 1,4 ± 0,7 x 10-5 M) como na ausência de epitélio funcional (CE50 = 1,5 ± 0,3 x 10-5 M), bem como anéis pré-contraídos com 18 ou 60 mM de KCl, apresentando valores de CE50 de 2,3 ± 0,4 x 10(7) M e 1,8 ± 0,8 x 10-5 M, respectivamente. Este efeito relaxante, sobre as contrações induzidas por 18 mM de KCl, tanto foi significantemente mais potente quanto mais eficaz quando comparado ao efeito sobre as contrações induzidas por 60 mM de KCl. Assim, labdano302 mostra um efeito relaxante em traquéia isolada de cobaia, tanto em seu tônus basal como sob estímulo contrátil, aparentemente sem a participação dos fatores relaxantes derivados do epitélio, contudo com possível participação dos canais de K+.

Xylopia langsdorfiana St. Hil. & Tul. is popularly known as "pimenteira-da-terra" in Southeast of Brazil. The fractionation of the ethanol extract obtained from the stem-bark of this species yielded a labdane-type diterpene identified as 8(17),12E,14-labdatrien-18-oic acid, referred here as labdane302. In this study, we investigated the effect of labdane302 in guinea-pig trachea. labdane302 relaxed the basal tonus of trachea rings with EC50 value of 6.7 ± 0.5 x 10-8 M. The diterpene labdane302 relaxed the pre-contracted rings by carbachol 10-6 M both in the presence (EC50 = 1.4 ± 0.7 x 10-5 M) and absence of functional epithelium (EC50 = 1.5 ± 0.3 x 10-5 M), as well as pre-contracted by KCl 18 mM or 60 mM, presented EC50 values of 2.3 ± 0.4 x 10-7 M and 1.8 ± 0.8 x 10-5 M, respectively. This relaxant effect, upon contractions induced by KCl 18 mM, was more potent as well as more efficient than the one presented with KCl 60 mM pre-contracted rings. The labdane-type diterpene labdane302 shows the relaxant effect in isolated guinea-pig trachea, pre-contracted or upon basal tonus, apparently without participation of epithelium-derived relaxant factors, but apparently involving activation of K+ channels.

Extratos metanólico e acetato de etila de Solanum megalonyx Sendtn. (Solanaceae) apresentam atividade espasmolítica em íleo isolado de cobaia: um estudo comparativo / Methanol and ethyl acetate extracts from Solanum megalonyx Sendtn. (Solanaceae) present spasmolytic activity in guinea-pig ileum: a comparative study

Solanum megalonyx Sendtn. (Solanaceae) é conhecida popularmente por "jurubeba" no Nordeste do Brasil e se apresenta na forma de arbusto. Várias espécies de Solanum apresentam efeito espasmolítico em órgãos isolados. Assim, objetivou-se investigar e comparar o efeito dos extratos metanólico (SM-MeOH) e acetato de etila (SM-AcOEt), obtidos das partes aéreas de S. megalonyx, em íleo isolado de cobaia. SM-MeOH e SM-AcOEt antagonizaram (n = 5) as contrações fásicas induzidas por 1 mM de acetilcolina (logCI50 = 3,2 ± 0,1 e 1,8 ± 0,6 mg/mL, respectivamente) ou de histamina (logCI50 = 2,8 ± 0,5 e 1,7 ± 0,3 mg/mL, respectivamente). SM-MeOH e SM-AcOEt também relaxaram (n = 5) o íleo pré-contraído por 40 mM de KCl (logCE50 = 1,9 ± 0,09 e 1,9 ± 0,1 mg/mL, respectivamente), por 1 mM de histamina (logCE50 = 1,9 ± 0,07 e 1,7 ± 0,08 mg/mL, respectivamente) ou de acetilcolina (logCE50 = 1,9 ± 0,02 e 1,7 ± 0,09 mg/mL, respectivamente) de maneira dependente de concentração e equipotente. Demonstra-se pela primeira vez que S. megalonyx apresenta efeito espasmolítico não seletivo em íleo isolado de cobaia, sugerindo que os extratos podem estar agindo em um passo comum da via de sinalização dos agentes contráteis testados.

Solanum megalonyx Sendtn. (Solanaceae) is known popularly as "jurubeba" in Northeastern Brazil where it can be found as a shrub. Several species of Solanum present spasmolytic effect in several tissues, thus this study was aimed to investigate and compare the effect of the methanol extract (SMMeOH) and ethyl acetate extract (SM-AcOEt), obtained from aerial parts of Solanum megalonyx Sendtn., in guinea-pig ileum. In this work, both SM-MeOH and SM-AcOEt antagonized the phasic contraction induced by acetylcholine 1 mM (logIC50 = 3.2 ± 0.1 and 1.8 ± 0.6 mg/mL) and histamine 1 mM (logIC50 = 2.8 ± 0.5 and 1.7 ± 0.3 mg/mL, respectively) (n = 5), without statistical differences between these values. In another set of experiments, SM-MeOH and SM-AcOEt also relaxed the isolated guinea-pig ileum pre-contracted by KCl 40 mM (logEC50 = 1.9 ± 0.09 and 1.9 ± 0.1 mg/mL, respectively), histamine 1 mM (logEC50 = 1.9 ± 0.07 mg/mL and 1.7 ± 0.08 mg/mL, respectively) or acetylcholine (logEC50 = 1.9 ± 0.02 mg/mL and 1.7 ± 0.09 mg/mL, respectively) (n = 5) in a concentration-dependent and equipotent manner. This study demonstrates for the first time that aerial parts of S. megalonyx present a non-selective spasmolytic effect in guinea-pig ileum, suggesting that the extracts could be acting in a common step of the pathway signaling that leads to contraction induced by the contractile agents tested.

Hepatitis B virus screening in contacts of blood donors with antibodies against core protein (anti-HBc), but without surface antigen (HBsAg)

To increase blood safety Brazil introduced screening for anti-HBc among blood donors in 1993. There was a decrease in the hepatitis B virus (HBV) transmission, but this measure identified a great number of HBsAg-negative, anti-HBc-positive donors. Surveillance policy determines that contacts of HBV carriers should be screened to HBV markers, but there is no recommendation about how to guide contacts of HBsAg-negative, anti-HBc-positive donors. Aiming to evaluate whether the contacts of this group are at greater risk for HBV infection, a cross-sectional study was performed to compare prevalence of HBV infection between contacts of HBsAg-positive blood donors (group I) and contacts of HBsAg-negative, anti-HBc-positive donors (group II). Contacts were submitted to a questionnaire and blood tests for HBV markers. In group I (n = 143), 53 (37.1 percent) were anti-HBc-positive and 11 (7.7 percent) were HBsAg-positive. In group II (n = 111), there were 9 and 0.9 percent, respectively. HBV exposure was associated with group I, sexual activity, blood transfusion, being one of the donor's parents, and living for more than ten years with the donor. Regarding the families as sample units, it was more common to find at least one member with HBV markers (p < 0.05) among the families of group I compared to group II. Contacts of HBsAg-negative, anti-HBc-positive individuals presented a much lower risk of having already been exposed to HBV and there is no need to screen them for HBV in low to moderate prevalence populations.

Púrpura em paciente com estrongiloidíase disseminada / Purpura in patient with disseminated strongiloidiasis

A infecção pelo Strongyloides stercoralis em associação com imunosupressão pode manifestar-se com lesões em múltiplos órgãos e sistemas, caracterizando a forma disseminada da doença. Lesões cutâneas não são freqüentemente relatadas e, se presentes, manifestam-se como rash e petéquias. Púrpuras bem definidas são pouco descritas. No presente trabalho é descrito um caso de estrongiloidíase disseminada, com acometimento cutâneo em forma de púrpura, que se desenvolveu em um paciente timectomizado e usuário crônico de corticosteróide devido à miastenia gravis.

Dapsone syndrome with acute renal failure during leprosy treatment: case report

Dapsone syndrome is a rare hypersensitivity reaction to dapsone and is characterized by high fever, papular or exfoliative dermatitis, progressing to liver toxicity and generalized lymphadenopathy, resembling a mononucleosis infection. We report a patient who developed acute renal failure, as well as other complications characteristic of dapsone syndrome, during leprosy treatment. Renal involvement had not been previously described as a dapsone syndrome feature.

Physical-chemical characteristics and fatty acids composition in dairy goat milk in response to roughage diet

O presente trabalho teve por objetivo avaliar o efeito de fontes de volumosos (feno de alfafa T1; feno de aveia T2 e silagem de milho T3) nas características físico-químicas (densidade, pH, acidez, teores de gordura, proteína, lactose e sólidos totais) e composição em ácidos graxos (C:4 a C:20) do leite. Foram utilizadas 9 cabras Saanen em lactação, em delineamento experimental triplo quadrado de Youden (3 animais x 2 períodos). Não houve efeito (P>0,05) dos tratamentos nas variáveis físico-químicas. Através de análise multivariada, verificou-se três padrões distintos de ácidos graxos: leite com maior quantidade de ácidos graxos de cadeia curta e dos ácidos C17:1w7 e C18:2w6 (T1); leite com quantidades equivalentes de ácidos graxos de cadeia curta, média e longa (T2) e leite com maiores quantidades dos ácidos C16:1w7, C17:0, C18:1w9 e C20:0 (T3). Estes resultados indicaram que a fonte de volumoso em dietas de cabras em lactação afeta a composição em ácidos graxos, sem alterar as características físico-químicas do leite. Os ácidos mais sensíveis aos efeitos dos tratamentos foram: C10:0, C12:0, C14:0, C16;0, C16:1w7, C18:0 e C18:3w6.

Imunoblot como teste suplementar para detecção de anticorpos contra o vírus da hepatite C em doadores de sangue / Immunoblot as a supplemental test to detect antibodies to hepatitis C virus in blood donors

Supplemental tests using Immunoblot are recommended to improve specificity of anti-HCV by ELISA. In Brazil immunoblot is not officially recommended. Aiming to identify EIA false-positive rate 70 positive EIA anti-HCV blood donors were submitted to 3rd generation immunoblot at Hemocentro of Mato Grosso State where polymerase chain reaction tests are not performed. There were 44 (62.9) immunoblot-positive, 22 (31.4) negative and 4 (5.7) indeterminate. Anti-HCV immunoblot can distinguish blood donors with false-positive ELISA from those who need medical assessment. Our data suggest that immunoblot could be useful in Brazilian blood banks where molecular biology tests are not available.

Hepatite Delta no Estado de Mato Grosso: apresentaçäo de cinco casos / Hepatitis Delta in the tate of Mato Grosso, Brazil: report of five cases

Cinco casos graves de infeccao pelos virus das hepatites B e D foram diagnosticados em jovens oriundos do norte do Mato Grosso, onde e comum a ocorrencia de hepatite B, mas nao de hepatite D. A proximidade com os Estados do Acre e do Amazonas e a migracao interna podem explicara introducao do virus da hepatite D na regiao. Os autores salientam a necessidade de manter vigilancia epidemiologica para casos de hepatite D na regiao

Disseminação linfática na endometriose colorretal / Lymphatic dissemination in colorectal endometriosis

A 40-year-old female patient presented abdominal pain during her periods and progressing constipation during the last 6 years. Retosigmoidoscopy showed a stenotic lesion 14cm above the anal verge. The patient underwent a retosigmoidectomy and the specimen was sent to histopathologic exam. The diagnose was endometriosis with the involvement of the colon and rectum, associated with dissemination of the endometrial cells by the lymph nodes. The patient recovered well and was discharged on the 6th postoperative day. It is commented on the rarity of the lymphatic dissemination in these cases and according to the reviewed literature, this is the 5th case reported. Thus, dissemination of the disease to extra-abdominal sites could possibly occur by this route. Therefore, pathologists should carefully exam the lymph nodes of the mesocolon in these specimens because findings of endometrial "metastasis" may be present.