Diffuse adult high-grade gliomas (HGGs) with necrosis encompass anaplastic oligodendrogliomas (AOs) with necrosis (grade III), glioblastomas (GBM, grade IV) and glioblastomas with an oligodendroglial component (GBMO, grade IV). Here, we aimed to search for prognostic relevance of histological classification and molecular alterations of these tumors. About 210 patients were included (63 AO, 56 GBM and 91 GBMO). GBMO group was split into "anaplastic oligoastrocytoma (AOA) with necrosis grade IV/GBMO," restricted to tumors showing intermingled astrocytic and oligodendroglial component, and "GBM/GBMO" based on tumors presenting oligodendroglial foci and features of GBM. Genomic arrays, IDH1 R132H expression analyses and IDH direct sequencing were performed. 1p/19q co-deletion characterized AO, whereas no IDH1 R132H expression and intact 1p/19q characterized both GBM and GBM/GBMO. AOA with necrosis/GBMO mainly demonstrated IDH1 R132H expression and intact 1p/19q. Other IDH1 or IDH2 mutations were extremely rare. Both histological and molecular classifications were predictive of progression free survival (PFS) and overall survival (OS) (P < 10(-4) ). Diffuse adult HGGs with necrosis can be split into three histomolecular groups of prognostic relevance: 1p/19q co-deleted AO, IDH1 R132H-GBM and 1p/19q intact IDH1 R132H+ gliomas that might be classified as IDH1 R132H+ GBM. Because of histomolecular heterogeneity, we suggest to remove the name GBMO.
Brain Neoplasms/diagnosis , Brain Neoplasms/genetics , Brain Neoplasms/metabolism , Glioma/diagnosis , Glioma/genetics , Glioma/metabolism , Adult , Brain Neoplasms/classification , Chromosome Aberrations , Chromosome Deletion , Chromosomes, Human, Pair 1/genetics , ErbB Receptors/genetics , Female , Follow-Up Studies , Gene Expression Profiling , Glioma/classification , Humans , Isocitrate Dehydrogenase/genetics , Ki-67 Antigen/metabolism , Male , Middle Aged , Mutation/genetics , Necrosis , Oligonucleotide Array Sequence Analysis , Prognosis , Survival Analysis
Kaposi's sarcoma-associated herpesvirus (KSHV), the causative agent of Kaposi's sarcoma is able to infect several cell types. By investigating hyperplastic lymph nodes from AIDS patients with multicentric Castleman's disease, we demonstrate, for the first time, by dual colour immunohistochemistry, that KSHV is frequently detectable in germinal centre macrophages. These macrophages, which display a latency programme and frequently contain apoptotic bodies, may represent a non-negligible reservoir for the virus in lymphoid organs.
AIDS-Related Opportunistic Infections/diagnosis , Castleman Disease/virology , Herpesvirus 8, Human/isolation & purification , Macrophages/virology , Sarcoma, Kaposi/diagnosis , Herpesvirus 8, Human/physiology , Humans , Immunohistochemistry/methods , Virus Latency
