Catalase-associated immune responses in plant-microbe interactions: A review.

Catalase, an enzyme central to maintaining redox balance and combating oxidative stress in plants, has emerged as a key player in plant defense mechanisms and interactions with microbes. This review article provides a comprehensive analysis of catalase-associated immune responses in plant-microbe interactions. It underscores the importance of catalase in plant defense mechanisms, highlights its influence on plant susceptibility to pathogens, and discusses its implications for understanding plant immunity and host-microbe dynamics. This review contributes to the growing body of knowledge on catalase-mediated immune responses and offers insights that can aid in the development of strategies for improved plant health and disease resistance.

Exploring the role of levan in plant immunity to pathogens: A review.

This review article delves into the intricate relationship between levan, a versatile polysaccharide, and its role in enhancing plant resistance against pathogens. By exploring the potential applications of levan in agriculture and biotechnology, such as crop protection, stress tolerance enhancement, and biotechnological innovations, significant advancements in sustainable agriculture are uncovered. Despite challenges in optimizing application methods and addressing regulatory hurdles, understanding the mechanisms of levan-mediated plant immunity offers promising avenues for future research. This review underscores the implications of utilizing levan to develop eco-friendly solutions, reduce reliance on chemical pesticides, and promote sustainable agricultural practices. Ultimately, by unraveling the pivotal role of levan in plant-pathogen interactions, this review sets the stage for transformative innovations in agriculture and highlights the path towards a more resilient and sustainable agricultural future.

Chitosan coating of seeds improves the germination and growth performance of plants: A Rreview.

This review article explores the fascinating world of chitosan coating applied to seeds and its profound impacts on enhancing the germination process and growth performance of plants. Chitosan, a biodegradable and non-toxic polysaccharide derived from chitin, has shown remarkable potential in seed treatment due to its bioactive properties. The review discusses the mechanisms of chitosan's effect on plant germination including promoting water uptake, enhancing nutrient absorption, and protecting seeds from biotic and abiotic stresses. Moreover, it evaluates the effects of chitosan on plant growth parameters such as root development, shoot growth, chlorophyll content, and overall yield. The review also discusses the sustainable aspects of chitosan coatings in agriculture, emphasizing their eco-friendly nature and potential for reducing reliance on synthetic chemicals. Overall, the findings underscore the significant benefits of chitosan-coated seeds in improving the overall performance of plants, paving the way for a greener and more productive agricultural future. Finally, the article will conclude with a SWOT analysis discussing the strengths, weaknesses, opportunities, and threats of this technology.

Genetic variations and gene expression profiles of Rice Black-streaked dwarf virus (RBSDV) in different host plants and insect vectors: insights from RNA-Seq analysis.

Rice black-streaked dwarf virus (RBSDV) is an etiological agent of a destructive disease infecting some economically important crops from the Gramineae family in Asia. While RBSDV causes high yield losses, genetic characteristics of replicative viral populations have not been investigated within different host plants and insect vectors. Herein, eleven publicly available RNA-Seq datasets from Chinese RBSDV-infected rice, maize, and viruliferous planthopper (Laodelphax striatellus) were obtained from the NCBI database. The patterns of SNP and RNA expression profiles of expected RBSDV populations were analyzed by CLC Workbench 20 and Geneious Prime software. These analyses discovered 2,646 mutations with codon changes in RBSDV whole transcriptome and forty-seven co-mutated hotspots with high variant frequency within the crucial regions of S5-1, S5-2, S6, S7-1, S7-2, S9, and S10 open reading frames (ORFs) which are responsible for some virulence and host range functions. Moreover, three joint mutations are located on the three-dimensional protein of P9-1. The infected RBSDV-susceptible rice cultivar KTWYJ3 and indigenous planthopper datasets showed more co-mutated hotspot numbers than others. Our analyses showed the expression patterns of viral genomic fragments varied depending on the host type. Unlike planthopper, S5-1, S2, S6, and S9-1 ORFs, respectively had the greatest read numbers in host plants; and S5-2, S9-2, and S7-2 were expressed in the lowest level. These findings underscore virus/host complexes are effective in the genetic variations and gene expression profiles of plant viruses. Our analysis revealed no evidence of recombination events. Interestingly, the negative selection was observed at 12 RBSDV ORFs, except for position 1015 in the P1 protein, where a positive selection was detected. The research highlights the potential of SRA datasets for analysis of the virus cycle and enhances our understanding of RBSDV's genetic diversity and host specificity.

Nature's coatings: Sodium alginate as a novel coating in safeguarding plants from frost damages.

Frost damage remains a significant challenge for agricultural practices worldwide, leading to substantial economic losses and food insecurity. Practically, traditional methods for frost management have proven ineffective and come with several drawbacks, such as energy consumption and limited efficacy. Hence, proposing an anti-freezing coating can be an innovative idea. The potential of sodium alginate (SA) to construct anti-freezing hydrogels has been explored in several sciences. SA hydrogels can form protective films around plants as a barrier against freezing temperatures and ice crystals on the plant's surface. Sodium alginate exhibits excellent water retention, enhancing plant hydration during freezing conditions. This coating can provide insulation, effectively shielding the plant from frost damage. The advantages of SA as a coating material, such as its biocompatibility, biodegradability, and non-toxic nature, are highlighted. Therefore, the proposed use of SA as an innovative coating material holds promise for safeguarding plants from frost damage. Following SA potential and frost's huge damage, the present review provides a comprehensive overview of the recent developments in SA-based anti-freezing hydrogels, their applications, and their potential in agriculture as anti-freezing coatings. However, further research and field trials are necessary to optimize the application methods and understand the long-term effects on productivity.

Genome mining conformance to metabolite profile of Bacillus strains to control potato pathogens.

Biocontrol agents are safe and effective methods for controlling plant disease pathogens, such as Fusarium solani, which causes dry wilt, and Pectobacterium spp., responsible for potato soft rot disease. Discovering agents that can effectively control both fungal and bacterial pathogens in potatoes has always presented a challenge. Biological controls were investigated using 500 bacterial strains isolated from rhizospheric microbial communities, along with two promising biocontrol strains: Pseudomonas (T17-4 and VUPf5). Bacillus velezensis (Q12 and US1) and Pseudomonas chlororaphis VUPf5 exhibited the highest inhibition of fungal growth and pathogenicity in both laboratory (48%, 48%, 38%) and greenhouse (100%, 85%, 90%) settings. Q12 demonstrated better control against bacterial pathogens in vivo (approximately 50%). Whole-genome sequencing of Q12 and US1 revealed a genome size of approximately 4.1 Mb. Q12 had 4413 gene IDs and 4300 coding sequences, while US1 had 4369 gene IDs and 4255 coding sequences. Q12 exhibited a higher number of genes classified under functional subcategories related to stress response, cell wall, capsule, levansucrase synthesis, and polysaccharide metabolism. Both Q12 and US1 contained eleven secondary metabolite gene clusters as identified by the antiSMASH and RAST servers. Notably, Q12 possessed the antibacterial locillomycin and iturin A gene clusters, which were absent in US1. This genetic information suggests that Q12 may have a more pronounced control over bacterial pathogens compared to US1. Metabolic profiling of the superior strains, as determined by LC/MS/MS, validated our genetic findings. The investigated strains produced compounds such as iturin A, bacillomycin D, surfactin, fengycin, phenazine derivatives, etc. These compounds reduced spore production and caused deformation of the hyphae in F. solani. In contrast, B. velezensis UR1, which lacked the production of surfactin, fengycin, and iturin, did not affect these structures and failed to inhibit the growth of any pathogens. Our findings suggest that locillomycin and iturin A may contribute to the enhanced control of bacterial pectolytic rot by Q12.

ß-glucan-induced disease resistance in plants: A review.

Systemic acquired resistance (SAR) and induced systemic resistance (ISR) are caused by various factors, including both pathogenic and non-pathogenic ones. ß-glucan primarily originates from bacteria and fungi, some species of these organisms work as biological agents in causing diseases. When ß-glucan enters plants, it triggers the defense system, leading to various reactions such as the production of proteins related to pathogenicity and defense enzymes. By extracting ß-glucan from disturbed microorganisms and using it as an inducing agent, plant diseases can be effectively controlled by activating the plant's defense system. ß-glucan plays a crucial role during the interaction between plants and pathogens. Therefore, modeling the plant-pathogen relationship and using the molecules involved in this interaction can help in controlling plant diseases, as pathogens have genes related to resistance against pathogenicity. Thus, it is reasonable to identify and use biological induction agents at a large scale by extracting these compounds.

The application of chitosan as a carrier for fertilizer: A review.

The smart combination of agriculture and other sciences can greatly reduce the limits of fertilizer use. Chitosan is a linear amino polysaccharide with a rigid structure which has hydrophilic and crystal properties. The formation of intermolecular hydrogen bonds the presence of reactive groups and cross-linking, the formation of salts with organic and inorganic acids with complexing and chelating properties ionic conductivity, film formation are the characteristics of chitosan. With the presence of amino groups, chitosan can form a complex with other compounds and also enter the vascular system of plants and lead to the activation of metabolic-physiological pathways of plants. This polymeric compound can bond with other natural polymers and in combination with fertilizers and nutritional elements, on the one hand, it can provide the nutritional needs of the plant and on the other hand, it also helps to improve the soil texture. Chitosan nanomaterials as a Next-generation fertilizers act as plant immune system enhancers through slow, controlled, and targeted delivery of nutrients to plants. Chitosan can assist agricultural researchers and has become an ideal and effective option with its many applications in various fields.

Bioremediation of Heavy Metals by Rhizobacteria.

Heavy elements accumulate rapidly in the soil due to industrial activities and the industrial revolution, which significantly impact the morphology, physiology, and yield of crops. Heavy metal contamination will eventually affect the plant tolerance threshold and cause changes in the plant genome and genetic structure. Changes in the plant genome lead to changes in encoded proteins and protein sequences. Consuming these mutated products can seriously affect human and animal health. Bioremediation is a process that can be applied to reduce the adverse effects of heavy metals in the soil. In this regard, bioremediation using plant growth-promoting rhizobacteria (PGPRs) as beneficial living agents can help to neutralize the negative interaction between the plant and the heavy metals. PGPRs suppress the adverse effects of heavy metals and the negative interaction of plant-heavy elements by different mechanisms such as biological adsorption and entrapment of heavy elements in extracellular capsules, reduction of metal ion concentration, and formation of complexes with metal ions inside the cell.

Microencapsulation of Bacillus velezensis Using Alginate-Gum Polymers Enriched with TiO2 and SiO2 Nanoparticles.

Bacillus bacteria are a group of plant growth stimulants that increase plant growth and resistance to plant pathogens by producing various metabolites. With their large surface area and small size, nanoparticles can be used in controlled-release formulations and increase the efficiency of the desired product. Encapsulation of biological agents in combination with nanoparticles can be an essential step in increasing the performance of these agents in adverse environmental conditions. In this study, which is the result of a collaboration between scientists from Italy and Iran, Bacillus velezensis was encapsulated in alginate combined with whey protein and zedo, mastic, and tragacanth gums in the presence of silica and titania nanoparticles to obtain two-layer and multilayer assemblies acting as novel, smart micro-encapsulation systems. The results of laboratory studies showed that the B. velezensis could produce protease, lipase, siderophore, auxin, and a dissolution of mineral phosphate. Scanning electron microscopy images (SEM) showed that the studied microcapsules were almost spherical. Moisture affinity, swelling, and efficiency of each microcapsule were examined. The results showed that the highest encapsulation efficiency (94.3%) was related to the multilayer formulation of alginate-whey protein-zedo. XRD and FTIR spectroscopy showed that the alginate, whey protein, and zedo were mixed properly and no incompatible composition occurred in the reaction. This study aimed to provide a suitable formulation of biofertilizers based on biodegradable compounds as an alternative to chemical fertilizers, which is low cost and very effective without harming humans and the environment.

Chitosan as a potential natural compound to manage plant diseases.

The necessity for non-chemical approaches has grown as awareness of the dangers posed by pesticides has spread. Chitosan, due to its biocompatibility, biodegradability, and bioactivity is one the effective choice in phytopathology. Chitosan is a biopolymer that reduces plant diseases through two main mechanisms: (1) Direct antimicrobial function against pathogens, including plasma membrane damage mechanisms, interactions with DNA and RNA (electrostatic interactions), metal chelating capacity, and deposition onto the microbial surface, (2) Induction of plant defense responses resulting from downstream signalling, transcription factor activation, gene transcription and finally cellular activation after recognition and binding of chitin and chitosan by cell surface receptors. This biopolymer have potential with capability to combating fungi, bacteria, and viruses phythopathogens. Chitosan is synthesized by deacetylating chitin. The degree of deacetylation and molecular weight of chitosan are variable and have been mentioned as important structural parameters in chitosan's biological properties. Chitosan with a higher degree of deacetylation (>70 %) has better biological properties. Many crops able to withstand pre- and post-harvest illnesses better after receiving chitosan as a seed treatment, soil amendment, or foliar spray. This review discussed the properties and use of chitosan and focuses on its application as a plant resistance inducer against pathogens.