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1.
PLoS Negl Trop Dis ; 15(8): e0009672, 2021 08.
Artículo en Inglés | MEDLINE | ID: mdl-34449764

RESUMEN

BACKGROUND: Understanding epidemiological variables affecting gametocyte carriage and density is essential to design interventions that most effectively reduce malaria human-to-mosquito transmission. METHODOLOGY/PRINCIPAL FINDINGS: Plasmodium falciparum and P. vivax parasites and gametocytes were quantified by qPCR and RT-qPCR assays using the same methodologies in 5 cross-sectional surveys involving 16,493 individuals in Brazil, Thailand, Papua New Guinea, and Solomon Islands. The proportion of infections with detectable gametocytes per survey ranged from 44-94% for P. falciparum and from 23-72% for P. vivax. Blood-stage parasite density was the most important predictor of the probability to detect gametocytes. In moderate transmission settings (prevalence by qPCR>5%), parasite density decreased with age and the majority of gametocyte carriers were children. In low transmission settings (prevalence<5%), >65% of gametocyte carriers were adults. Per survey, 37-100% of all individuals positive for gametocytes by RT-qPCR were positive by light microscopy for asexual stages or gametocytes (overall: P. falciparum 178/348, P. vivax 235/398). CONCLUSIONS/SIGNIFICANCE: Interventions to reduce human-to-mosquito malaria transmission in moderate-high endemicity settings will have the greatest impact when children are targeted. In contrast, all age groups need to be included in control activities in low endemicity settings to achieve elimination. Detection of infections by light microscopy is a valuable tool to identify asymptomatic blood stage infections that likely contribute most to ongoing transmission at the time of sampling.


Asunto(s)
Malaria Falciparum/parasitología , Malaria Vivax/parasitología , Plasmodium falciparum/aislamiento & purificación , Plasmodium vivax/aislamiento & purificación , Adolescente , Enfermedades Asintomáticas , Brasil/epidemiología , Niño , Preescolar , Estudios Transversales , Femenino , Humanos , Lactante , Malaria Falciparum/epidemiología , Malaria Falciparum/transmisión , Malaria Vivax/epidemiología , Malaria Vivax/transmisión , Masculino , Papúa Nueva Guinea/epidemiología , Plasmodium falciparum/genética , Plasmodium falciparum/crecimiento & desarrollo , Plasmodium falciparum/fisiología , Plasmodium vivax/genética , Plasmodium vivax/crecimiento & desarrollo , Plasmodium vivax/fisiología , Tailandia/epidemiología , Adulto Joven
2.
Malar J ; 19(1): 319, 2020 Sep 03.
Artículo en Inglés | MEDLINE | ID: mdl-32883308

RESUMEN

BACKGROUND: The use of molecular diagnostics has revealed an unexpectedly large number of asymptomatic low-density malaria infections in many malaria endemic areas. This study compared the gains in parasite prevalence obtained by the use of ultra-sensitive (us)-qPCR as compared to standard qPCR in cross-sectional surveys conducted in Thailand, Brazil and Papua New Guinea (PNG). The compared assays differed in the copy number of qPCR targets in the parasite genome. METHODS: Plasmodium falciparum (Pf) and Plasmodium vivax (Pv) parasites were quantified by qPCR amplifying the low-copy Pf_ and Pv_18S rRNA genes or the multi-copy targets Pf_varATS and Pv_mtCOX1. Cross-sectional surveys at the three study sites included 2252 participants of all ages and represented different transmission intensities. RESULTS: In the two low-transmission areas, P. falciparum positivity was 1.3% (10/773) (Thailand) and 0.8% (5/651) (Brazil) using standard Pf_18S rRNA qPCR. In these two countries, P. falciparum positivity by Pf_varATS us-qPCR increased to 1.9% (15/773) and 1.7% (11/651). In PNG, an area with moderate transmission intensity, P. falciparum positivity significantly increased from 8.6% (71/828) by standard qPCR to 12.2% (101/828) by us-qPCR. The proportions of P. falciparum infections not detected by standard qPCR were 33%, 55% and 30% in Thailand, Brazil and PNG. Plasmodium vivax was the predominating species in Thailand and Brazil, with 3.9% (30/773) and 4.9% (32/651) positivity by Pv_18S rRNA qPCR. In PNG, P. vivax positivity was similar to P. falciparum, at 8.0% (66/828). Use of Pv_mtCOX1 us-qPCR led to a significant increase in positivity to 5.1% (39/773), 6.4% (42/651) and 11.5% (95/828) in Thailand, Brazil, and PNG. The proportions of P. vivax infections missed by standard qPCR were similar at all three sites, with 23%, 24% and 31% in Thailand, Brazil and PNG. CONCLUSION: The proportional gains in the detection of P. falciparum and P. vivax infections by ultra-sensitive diagnostic assays were substantial at all three study sites. Thus, us-qPCR yields more precise prevalence estimates for both P. falciparum and P. vivax at all studied levels of endemicity and represents a significant diagnostic improvement. Improving sensitivity in P. vivax surveillance by us-qPCR is of particular benefit, because the additionally detected P. vivax infections signal the potential presence of hypnozoites and subsequent risk of relapse and further transmission.


Asunto(s)
Estudios Transversales/métodos , Malaria Falciparum/epidemiología , Malaria Vivax/epidemiología , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa/métodos , Brasil/epidemiología , Malaria Falciparum/transmisión , Malaria Vivax/transmisión , Papúa Nueva Guinea/epidemiología , Plasmodium falciparum/aislamiento & purificación , Plasmodium vivax/aislamiento & purificación , Prevalencia , Sensibilidad y Especificidad , Tailandia/epidemiología
3.
Nat Med ; 26(5): 741-749, 2020 05.
Artículo en Inglés | MEDLINE | ID: mdl-32405064

RESUMEN

A major gap in the Plasmodium vivax elimination toolkit is the identification of individuals carrying clinically silent and undetectable liver-stage parasites, called hypnozoites. This study developed a panel of serological exposure markers capable of classifying individuals with recent P. vivax infections who have a high likelihood of harboring hypnozoites. We measured IgG antibody responses to 342 P. vivax proteins in longitudinal clinical cohorts conducted in Thailand and Brazil and identified candidate serological markers of exposure. Candidate markers were validated using samples from year-long observational cohorts conducted in Thailand, Brazil and the Solomon Islands and antibody responses to eight P. vivax proteins classified P. vivax infections in the previous 9 months with 80% sensitivity and specificity. Mathematical models demonstrate that a serological testing and treatment strategy could reduce P. vivax prevalence by 59-69%. These eight antibody responses can serve as a biomarker, identifying individuals who should be targeted with anti-hypnozoite therapy.


Asunto(s)
Biomarcadores/sangre , Malaria Vivax/diagnóstico , Pruebas Serológicas/métodos , Adulto , Brasil/epidemiología , Niño , Estudios de Cohortes , Diagnóstico Precoz , Humanos , Inmunoglobulina G/análisis , Inmunoglobulina G/sangre , Control de Infecciones/métodos , Estudios Longitudinales , Malaria Vivax/sangre , Malaria Vivax/epidemiología , Melanesia/epidemiología , Plasmodium vivax/fisiología , Prevalencia , Sensibilidad y Especificidad , Pruebas Serológicas/normas , Tailandia/epidemiología , Factores de Tiempo
4.
PLoS Negl Trop Dis ; 11(9): e0005888, 2017 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-28892517

RESUMEN

Plasmodium vivax remains an important cause of malaria in South America and the Asia-Pacific. Naturally acquired antibody responses against multiple P. vivax proteins have been described in numerous countries, however, direct comparison of these responses has been difficult with different methodologies employed. We measured antibody responses against 307 P. vivax proteins at the time of P. vivax infection, and at 2-3 later time-points in three countries. We observed that seropositivity rates at the time of infection were highest in Thailand, followed by Brazil then PNG, reflecting the level of antigenic input. The majority of sero-reactive antigens in all sites induced short-lived antibody responses with estimated half-lives of less than 6 months, although there was a trend towards longer-lived responses in PNG children. Despite these differences, IgG seropositivity rates, magnitude and longevity were highly and significantly rank-correlated between the different regions, suggesting such features are reflective of the individual protein.


Asunto(s)
Formación de Anticuerpos , Antígenos de Protozoos/inmunología , Malaria Vivax/inmunología , Plasmodium vivax/inmunología , Proteínas Protozoarias/inmunología , Adolescente , Adulto , Anciano , Anticuerpos Antiprotozoarios/sangre , Brasil , Niño , Preescolar , Femenino , Semivida , Humanos , Inmunoglobulina G/sangre , Malaria Vivax/parasitología , Masculino , Persona de Mediana Edad , Papúa Nueva Guinea , Tailandia , Adulto Joven
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