Effects of growth hormone-releasing hormone agonistic analog MR-409 on insulin-secreting cells under cyclopiazonic acid-induced endoplasmic reticulum stress.

The endoplasmic reticulum (ER) stress is one of the mechanisms related to decreased insulin secretion and beta cell death, contributing to the progress of type 2 diabetes mellitus (T2D). Thus, investigating agents that can influence this process would help prevent the development of T2D. Recently, the growth-hormone-releasing hormone (GHRH) action has been demonstrated in INS-1E cells, in which it increases cell proliferation and insulin secretion. As the effects of GHRH and its agonists have not been fully elucidated in the beta cell, we proposed to investigate them by evaluating the role of the GHRH agonist, MR-409, in cells under ER stress. Our results show that the agonist was unable to ameliorate or prevent ER stress. However, cells exposed to the agonist showed less oxidative stress and greater survival even under ER stress. The mechanisms by which GHRH agonist, MR-409, leads to these outcomes require further investigation.

Quality of life of people with chronic ulcers.

INTRODUCTION: Patients with chronic ulcers have physical, social, and psychological changes that directly affect their quality of life. OBJECTIVES: To evaluate the quality of life and analyze the association between personal and health characteristics in patient quality of life for those with chronic ulcers. METHOD: A cross-sectional study, nonprobabilistic. The questionnaire to evaluate personal and health characteristics and the Ferrans and Power Quality of Life Index-Wound Version were used for the data collection. The sample consisted of 200 patients with chronic ulcers of several etiologies (venous ulcers, pressure ulcers, diabetic ulcers, and mixed) recruited at the basic health units in two cities in the South of Minas Gerais, Brazil. RESULTS: Overall, the study found that their quality of life was rated as good. There was a positive correlation between the different ages (P = 0.0165), number of children (P = 0.0083), and practice of religion (P = 0.0394) with quality of life.

Fatores ambientais e parâmetros genéticos para características reprodutivas de fêmeas Nelore na região meio norte do Brasil

The study was carried out to evaluate the age-at-first-calving and calving interval (IPP and IDP) in Nelore cows, using IPP and IDP data from 23,066 females reared in a grazing regime in the States of Maranhão and Piauí from 1976 to 2012. Data were analyzed statistically using the SAS (Statistical Analysis System) program. The mean and coefficient of variation for the age-at-first-calving was 1252.77 ± 125 days (10.41 months; 10.56%) and, for the calving interval, values were 591.73 ± 149.91 days (12.49 months; 38.74%). Uncharacteristic analyses were used to estimate genetic parameters for heritability values, obtaining values of approximately 0.026 and 0.006 for IPP and IDP, respectively. The repeatability estimate for the IDP characteristic, as calculated by uncharacteristic analysis, had a generally low value of 0.025. Similarly, for trait analysis, heritability values were 0.03 and 0.06 for IPP and IDP, respectively, and the estimated repeatability coefficient was 0.14. The genetic correlations between the reproductive characteristics (IPP and IDP) observed in this study were equal to 0.06. The reproductive performance of the herd studied, in tropical climate conditions, can be considered to be satisfactory, in accordance with the parameters obtained by other authors in different regions.

O estudo foi conduzido para avaliar a idade ao primeiro parto (IPP) e o intervalo de partos (IDP) em fêmeas da raça Nelore utilizando 23.066 registros de IPP e IDP de fêmeas criadas em regime de pasto nos estados do Maranhão e Piauí no período de 1976 a 2012. As análises foram realizadas utilizando modelo animal uni e bicaracterísticas para IPP e IDP. As médias obtidas e os coeficientes de variação para as características de idade ao primeiro parto foram 1252,77±125 dias (41,76 meses) a 10,56%; intervalo de partos 591,729±149,91 dias (19,72 meses) a 38,74%, respectivamente. Foram estimados parâmetros genéticos em análise unicaracterística, sendo obtidos valores de herdabilidade em torno de 0,026 e 0,006 para IPP e IDP, respectivamente. A estimativa de repetibilidade para as características IDP em análise unicaracterística apresentou-se, de forma geral, com baixa magnitude 0,025. Na mesma ordem, para análise bicaracterística, as herdabilidades foram de 0,03 e 0,06 para IPP e IDP, e o coeficiente de repetibilidade estimado de 0,14, respectivamente. As correlações genéticas entre as características reprodutivas (IPP e IDP) observadas neste trabalho foram iguais a 0,06. O desempenho reprodutivo do rebanho estudado, para as condições de clima tropical, pode ser considerado satisfatório, estando em acordo com os parâmetros encontrados por outros autores em diferentes regiões.

Fatores ambientais e parâmetros genéticos para características reprodutivas de fêmeas Nelore na região meio norte do Brasil

The study was carried out to evaluate the age-at-first-calving and calving interval (IPP and IDP) in Nelore cows, using IPP and IDP data from 23,066 females reared in a grazing regime in the States of Maranhão and Piauí from 1976 to 2012. Data were analyzed statistically using the SAS (Statistical Analysis System) program. The mean and coefficient of variation for the age-at-first-calving was 1252.77 ± 125 days (10.41 months; 10.56%) and, for the calving interval, values were 591.73 ± 149.91 days (12.49 months; 38.74%). Uncharacteristic analyses were used to estimate genetic parameters for heritability values, obtaining values of approximately 0.026 and 0.006 for IPP and IDP, respectively. The repeatability estimate for the IDP characteristic, as calculated by uncharacteristic analysis, had a generally low value of 0.025. Similarly, for trait analysis, heritability values were 0.03 and 0.06 for IPP and IDP, respectively, and the estimated repeatability coefficient was 0.14. The genetic correlations between the reproductive characteristics (IPP and IDP) observed in this study were equal to 0.06. The reproductive performance of the herd studied, in tropical climate conditions, can be considered to be satisfactory, in accordance with the parameters obtained by other authors in different regions.

O estudo foi conduzido para avaliar a idade ao primeiro parto (IPP) e o intervalo de partos (IDP) em fêmeas da raça Nelore utilizando 23.066 registros de IPP e IDP de fêmeas criadas em regime de pasto nos estados do Maranhão e Piauí no período de 1976 a 2012. As análises foram realizadas utilizando modelo animal uni e bicaracterísticas para IPP e IDP. As médias obtidas e os coeficientes de variação para as características de idade ao primeiro parto foram 1252,77±125 dias (41,76 meses) a 10,56%; intervalo de partos 591,729±149,91 dias (19,72 meses) a 38,74%, respectivamente. Foram estimados parâmetros genéticos em análise unicaracterística, sendo obtidos valores de herdabilidade em torno de 0,026 e 0,006 para IPP e IDP, respectivamente. A estimativa de repetibilidade para as características IDP em análise unicaracterística apresentou-se, de forma geral, com baixa magnitude 0,025. Na mesma ordem, para análise bicaracterística, as herdabilidades foram de 0,03 e 0,06 para IPP e IDP, e o coeficiente de repetibilidade estimado de 0,14, respectivamente. As correlações genéticas entre as características reprodutivas (IPP e IDP) observadas neste trabalho foram iguais a 0,06. O desempenho reprodutivo do rebanho estudado, para as condições de clima tropical, pode ser considerado satisfatório, estando em acordo com os parâmetros encontrados por outros autores em diferentes regiões.