Preweaning piglet survival on commercial farms.

Preweaning piglet mortality (PWM), a trait highly related to litter size, is one of the main concerns associated with productive efficiency and animal welfare in commercial pig farms. The objectives of this work were to study piglet survival at the farm level, to establish a survival rate (SR) as a target indicator to be improved, and to model it based on other reproductive parameters. Analyzed data corresponded to 580 Spanish commercial farms with a total inventory of 809,768 sows. These farms showed a mean SR of 85.70% piglets born alive (BA), which decreased to 81.81% when total piglets born (TB) were considered. The SR was strongly associated with prolificacy (P < 0.01), the parities with the highest prolificacy being those that had the lowest SR. Thus, the highest correlations were for the SR of piglets BA in the third and fourth parities (r = -0.460 and r = -0.452, respectively, P < 0.01), and for the SR of piglets TB in the fourth parity (r = -0.546, P < 0.01), which was the one with the highest prolificacy. The values corresponding to the quartile of farms with the highest SR within the most productive farms were established as targets to be improved, which were ≥88.5% of piglets BA and 83.2% of piglets TB. Nevertheless, the direct associations shown between the piglet's survival and prolificacy and other productive factors, such as the age of piglets at weaning, the farrowings per sow and year and the farrowing interval, suggest the convenience of modeling the risk of PWM on farms to have its own target of survival index to be improved.

Sow prolificacy and preweaning piglet mortality have increased parallelly on commercial farms. This loss of piglets is a concern of efficiency and animal welfare, and it requires the improvement of piglet survival by reducing the number of stillborn piglets and piglet mortality during lactation, paying particular attention to hyperprolific sows (≥15 total piglets born per litter). Data from 580 commercial farms with an average inventory of 809,768 sows have been analyzed to propose two predictive models based on several reproductive parameters and two survival rate targets with the aim of reducing this problem, which are ≥88.5% of piglets born alive and ≥83.2% of total piglets born.

Multi-Criteria Evaluation Model of Management for Weaned Piglets and Its Relations with Farm Performance and Veterinary Medicine Consumption.

Weaned piglets, being immature, demand careful handling to mitigate post-weaning stress in order to avoid immunosuppression and the use of antimicrobials to palliate the effects of disease outbreaks due to poor management. The objective of this work is to design a quick scan calculator or multi-criteria evaluation model of management for weaned piglets, founded on 10 critical indices covering post-weaning management aspects based on hygienic measures and management of facilities and animals. These include pre-weaning handling, batch management, biosecurity, water management, feed management, health program, stockmen training, temperature, ventilation, and floor conditions and density to relate handling and hygiene practices with farm performance and the consumption of veterinary medication. Each index carries a maximum score of ten, with evaluations derived from different management factors that make up each index (from three to eight factors were evaluated per index). Their cumulative score reflects the degree of adequacy of on-farm management. Therefore, a perfectly managed farm would achieve 100 points. The calculator underwent testing on 23 intensive farms with a total population of close to 16,000 sows and more than 400,000 weaned piglets, revealing the highest mean scores in floor conditions and density (8.03 out of 10) and pre-weaning handling and health programs (6.87 and 6.28, respectively). Conversely, the lowest scores corresponded to temperature, ventilation, water management, and stockmen training (4.08, 4.32, 4.81, and 4.93, respectively). The assessed farms averaged a global score of 56.12 out of 100 (from 37.65 to 76.76). The calculator's global score correlated with key post-weaning productivity and piglet health indicators, such as the feed conversion ratio, mortality rate, and piglet production cost, with r values of -0.442, -0.437, and -0.435, respectively (p < 0.05). Additionally, it negatively correlated with medication costs per piglet (r = -0.414; p < 0.05) and positively with annual farm productivity (r = 0.592; p < 0.01). To enhance management, hygiene, and prevention, farms should prioritize addressing indices with the lowest scores, thereby reducing medication consumption and enhancing productivity and health outcomes. Additionally, this quick scan calculator can be used for benchmarking purposes.

Detection of Mycobacterium tuberculosis complex field infections in cattle using fecal volatile organic compound analysis through gas chromatography-ion mobility spectrometry combined with chemometrics.

Bovine tuberculosis is considered a re-emerging disease caused by different species from the Mycobacterium tuberculosis complex (MTC), important not only for the livestock sector but also for public health due to its zoonotic character. Despite the numerous efforts that have been carried out to improve the performance of the current antemortem diagnostic procedures, nowadays, they still pose several drawbacks, such as moderate to low sensitivity, highlighting the necessity to develop alternative and innovative tools to complement control and surveillance frameworks. Volatilome analysis is considered an innovative approach which has been widely employed in animal science, including animal health field and diagnosis, due to the useful and interesting information provided by volatile metabolites. Therefore, this study assesses the potential of gas chromatography coupled to ion mobility spectrometry (GC-IMS) to discriminate cattle naturally infected (field infections) by MTC from non-infected animals. Volatile organic compounds (VOCs) produced from feces were analyzed, employing the subsequent information through chemometrics. After the evaluation of variable importance for the projection of compounds, the final discriminant models achieved a robust performance in cross-validation, as well as high percentages of correct classification (>90%) and optimal data of sensitivity (91.66%) and specificity (99.99%) in external validation. The tentative identification of some VOCs revealed some coincidences with previous studies, although potential new compounds associated with the discrimination of infected and non-infected subjects were also addressed. These results provide strong evidence that a volatilome analysis of feces through GC-IMS coupled to chemometrics could become a valuable methodology to discriminate the infection by MTC in cattle. IMPORTANCE Bovine tuberculosis is endemic in many countries worldwide and poses important concerns for public health because of their zoonotic condition. However, current diagnostic techniques present several hurdles, such as low sensitivity and complexity, among others. In this regard, the development of new approaches to improve the diagnosis and control of this disease is considered crucial. Volatile organic compounds are small molecular mass metabolites which compose volatilome, whose analysis has been widely employed with success in different areas of animal science including animal health. The present study seeks to evaluate the combination of fecal volatilome analysis with chemometrics to detect field infections by bovine tuberculosis (Mycobacterium tuberculosis complex) in cattle. The good robust performance of discriminant models as well as the optimal data of sensitivity and specificity achieved highlight volatilome analysis as an innovative approach with huge potential.

Husbandry practices associated with extensification in European pig production and their effects on pork quality.

This review has been developed as part of the mEATquality project with the main objective to examine the types of extensification practices used in European pig husbandry and their effect on intrinsic meat quality. Literature search has resulted in 679 references in total, from which 53 showed a strict compliance with the goals of this review: 1) the use of local European breeds and their crossbreds (22 papers); 2) addition of forage to diet (9 papers); 3) increased space allowance (3 papers); 4) enrichment of environment (19 papers). The evaluation of selected extensification factors showed that not all of them have a clear impact on meat quality, and are often confounded. The most clear differences were observed when comparing autochthonous with commercial breeds, and systems with access to pastures or woodlands vs. indoor housing. Despite many studies focusing on the extensification of husbandry practices, some of the factors cannot be confirmed to have a direct effect on pork intrinsic quality.

Preliminary Feasibility of Near-Infrared Spectroscopy to Authenticate Grazing in Dairy Goats through Milk and Faeces Analysis.

Consumers are increasingly prone to request information about the production systems of the food they buy. For this purpose, certification and authentication methodologies are necessary not only to protect the choices of consumers, but also to protect producers and production systems. The objective of this preliminary work was to authenticate the grazing system of dairy goats using Near-Infrared Spectroscopy (NIRS) analyses of milk and faeces of the animals. Spectral information and several mathematical pre-treatments were used for the development of six discriminant models based on different algorithms for milk and faeces samples. Results showed that the NIRS spectra of both types of samples had some differences when the two feeding regimes were compared. Therefore, good discrimination rates were obtained with both strategies (faeces and milk samples), with classification percentages of up to 100% effectiveness. Discrimination of feeding regime and grazing authentication based on NIRS analysis of milk samples and an alternative sample such as faeces is considered as a potential approach for dairy goats and small ruminant production.

Use of polypyrrole ferrite microparticles and liquid chromatography-mass spectrometry for testing natural grass contamination by multiclass mycotoxins.

An analytical methodology based on the combination of dispersive magnetic solid-phase extraction (DMSPE) and liquid chromatography-mass spectrometry (LC-MS) is proposed to explore the occurrence of 13 mycotoxins (aflatoxins B1, G1, B2, and G2; deoxynivalenol; T-2 toxin; ochratoxin A; HT-2 toxin; enniatins A, A1, B, and B2; and beauvericin) and their derivatives in natural grass samples. Magnetic microparticles (Fe3O4) coated with polypyrrole (PPy) polymer were used in DMSPE sample treatment as adsorbent phase, and Fourier-transform infrared spectroscopy, field emission scanning electron microscopy, and energy dispersive X-ray spectroscopy have been used for its characterization. The experimental parameters influencing the adsorption and desorption steps of DMSPE have been optimized. Method validation has been carried out obtaining limits of quantification between 0.07 and 92 µg kg-1 corresponding to enniatin B or A1 and DON, respectively. A total of 83 natural grass samples from 8 dehesa farms were analysed. Enniatin B was found in all the samples (0.29 to 488 µg kg-1 concentration range) followed by enniatin B1 (92.8% of the samples) with a 0.12-137 µg kg-1 concentration range. Moreover, co-occurrence of mycotoxins was studied and between 2 and 5 mycotoxins appeared simultaneously in 97.6% of the samples. Distribution of the contamination according to natural grass location was also investigated.

Antiparasitic Tannin-Rich Plants from the South of Europe for Grazing Livestock: A Review.

Internal parasites are one of the main causes of health threats in livestock production, especially in extensive livestock farming. Despite the environmental toxic effects (loss of dung beetles, biodiversity, and other issues) and resistance phenomenon derived from their prolonged use, anti-parasitic chemical pharmaceuticals are frequently used, even in organic farming. Such a situation within the context of climate change requires urgent exploration of alternative compounds to solve these problems and apparent conflicts between organic farming objectives regarding the environment, public health, and animal health. This review is focused on some plants (Artemisia spp., Cichorium intybus L., Ericaceae family, Hedysarum coronarium L., Lotus spp., Onobrychis viciifolia Scop.) that are well known for their antiparasitic effect, are voluntarily grazed and ingested, and can be spontaneously found or cultivated in southern Europe and other regions with a Mediterranean climate. The differences found between effectiveness, parasite species affected, in vitro/in vivo experiments, and active compounds are explored. A total of 87 papers where antiparasitic activity of those plants have been studied are included in this review; 75% studied the effect on ruminant parasites, where gastrointestinal nematodes were the parasite group most studied (70%), and these included natural (31%) and experimental (37%) infections.

Feed Supplementation Detection during the Last Productive Stage of the Acorn-Fed Iberian Pig through a Faecal Volatilome Analysis.

The acorn-fed Iberian pig is known worldwide due to the quality of the resulting products commercialized after a natural and free grazing period of fattening in the dehesa agroforestry ecosystem. The quality regulation of the pig breed reserves "acorn" denomination for only those products obtained from animals exclusively fed grazing acorns and other natural resources; however, sometimes, feed supplementation of the pig's diet is fraudulently employed to reach an earlier slaughtering weight and to increase pig stocking rate, a strategy called postre (meaning "feed supplement"). In this sense, although many studies focused on Iberian pig diet have been published, the field detection of feed use for acorn-fed pig during the last finishing stage foraging in the dehesa, a practice which clashes with the official regulation, has not been explored yet. The present study employs a volatilome analysis (gas chromatography coupled to ion mobility spectrometry) of a non-invasive biological sample (faeces) to discriminate the grazing diet of only natural resources, that acorn-fed Iberian pigs are supposed to have, from those pigs that are also supplemented with feed. The results obtained show the suitability of the methodology used and the usefulness of the information obtained from faeces samples to discriminate and detect the fraudulent use of feed for acorn-fed Iberian pig fattening: a classification success ranging between 86.4% and 100% was obtained for the two chemometric approaches evaluated. These, together with the results of discriminant models, are discussed, in addition to the importance that the methodology optimized implies for the Iberian pig sector and market, which is also introduced. This methodology could be adapted to control organic farming animals or other upstanding livestock production systems which are supposed to be fully dependent on a natural grazing diet.

Geographical origin, curing plant and commercial category discrimination of cured Iberian hams through volatilome analysis at industry level.

Cured Iberian ham is a worldwide known product due to its high quality. Nowadays, there is a lack of official analytical methods to differentiate geographical origin (Protected Designation of Origin (PDO)), the curing plant where hams are processed, as well as the commercial categories in each industry. In this work, volatile organic compounds (VOCs) extracted from 998 Iberian hams were analyzed by Gas Chromatography coupled to Ion Mobility Spectrometry (GC-IMS), using the subsequent information to design discriminant models. High classification percentages were obtained for the three objectives of the study: 98,5% for geographical origin, 93,5% and 100% for curing plant discrimination, and an average rate of 84,5% for commercial category discrimination in the seven curing plants subject to study. Differences obtained in discriminant models are probably due to the complexity of Iberian ham manufacturing process. In this sense, the results obtained in the present study suggest slight differences between geographical areas and industries evaluated, even covered by the same PDO. Also, those differences may be related to the existing variability in terms of breed purity and feeding regime of Iberian pigs, which are two of the main determining factors of ham aroma.

The Role of Women on Dairy Goat Farms in Southern Spain.

One of the factors involved in goat milk production is the role of women as farmers. The aim of this study was to evaluate the role of women on dairy goat farms, considering: (1) the profile of women occupationally involved, (2) the organization of the women's work, (3) the degree of involvement by women in the decision-making on these farms, and (4) the influence of women's work on productive results. This study was conducted on 52 dairy goat farms in southern Spain. A descriptive analysis and means comparisons were performed to describe the farms where any women were involved or not. In 61.5% of the farms, at least one woman was involved, with an age of 42.2 ± 8.8 years. Very few women were farm owners, although women took binding decisions in 81.25% of these farms. Their work is dedicated to milking and caring for the kids. Women had a positive influence on the productive variables analysed, and for mastitis in herds, the incidence was lower in herds where women participated (p < 0.01). In conclusion, it is recommended to include women's work as a factor when characterizing dairy goats farms' systems to evaluate their positive effect on a farm's performance.

A New Approach to Detecting and Measuring Changes in the Feeding Behaviour Habits of Group-Housed Growing-Finishing Pigs.

The present work aims to estimate the methods of repeatability and of a new non-parametric approach based on typifying individuals into classes and quantifying (%) the pigs in a group that show similar feeding behaviour habits (FBHs) in consecutive periods ("maintenance"). Both methods were estimated over six consecutive 14-day periods in two trials of group-housed growing-finishing pigs (n = 60 each). The first trial started in summer and ended in autumn, and pigs were fed a pelleted diet (HT-P). The second trial started in spring and ended in summer, and the same diet was fed mash (TH-M). The average daily feed intake obtained the lowest repeatability and maintenance values, and it progressively decreased as pigs grew, independent of environmental conditions or physical feed form, whereas the maintenance and repeatability of the number of feeder visits and the visit size decreased when environmental conditions changed from temperate to hot, and mash-fed pigs had higher maintenance and repeatability values for the time spent eating than pellet-fed pigs. In conclusion, the new approach (maintenance) is a tool that is complementary to the classic repeatability concept and is useful for analysing the evolution of FBHs across periods of time at the individual level.

The Feeding Behaviour Habits of Growing-Finishing Pigs and Its Effects on Growth Performance and Carcass Quality: A Review.

Based on the available data of feeding behaviour habits (FBHs), this work aimed to discuss which type of pig, according to its FBHs, performs better and is more efficient. As pigs grow, average daily feed intake, meal size, and feeding rate increase, whereas small variations or even decreases in time spent eating and daily feeder visits have been reported. Moreover, the sex, breed, space allowance, feeder design, feed form, diet composition, and environmental conditions modify FBHs. On the other hand, the literature indicates the existence of four types of pigs: pigs that eat their daily feed intake in many short meals (nibblers) or in few large meals (meal eaters) combined with eating fast (faster eaters) or slow (slow eaters). The available scientific literature about ad libitum fed pigs suggests that pigs eating faster with bigger meals eat more, gain more weight, and are fatter than pigs eating less, slower, and with smaller meals. However, the feeding rate and the meal size do not influence feed efficiency. In conclusion, studies comparing growing-finishing pigs with similar feed intake, but different feeding rate and meal size are needed to better understand the influence of FBHs on feed efficiency.

Effect of Non-Genetic Factors on Reproduction of Extensive versus Intensive Florida Dairy Goats.

The main objective of this study was to evaluate the effect of the production system and other environmental/phenotype factors on age at first kidding (AFK), kidding interval (KI) and prolificacy of 19,772 Florida goats reared between 2000 and 2019 on 49 dairy farms (38 farms intensively managed and 11 extensively managed with grazing). AFK was lower on intensive (490.2 ± 0.9 days; n = 13,345) than on extensive farms (511.7 ± 2.5 days; n = 2357; p < 0.001), and highest during the spring season (533.9 ± 2.7 days; n = 1932; p < 0.001) in both production systems. The average KI was 355.7 ± 0.4 days, mainly varying according to dry period, kidding season and lactation number and kidding type (p < 0.01). A significant interaction between production system, kidding season and dry period was observed with the highest AFK on intensive farms during spring and summer for goats presenting a dry period of up to six months. The overall prolificacy (1.64 ± 0.01) increased in recent years in both systems, and it was affected by the production system, but with different patterns; so, the highest prolificacy of primiparous and multiparous goats was observed on extensive and intensive farms, respectively. Besides that, the prolificacy and other reproductive parameters, such as AFK, significantly increased in the last decade, which could be related to management improvements. Besides that, the existence of inter-annual variations should be considered to compare data between farms and years, and to establish the farms' objectives according to their production systems and production goals.

Review of the Methodologies for Measurement of Greenhouse Gas Emissions in Livestock Farming: Pig Farms as a Case of Study.

The global emission and accumulation of gases due to livestock farming is estimated to contribute to about 14.5% of the global warming effect due to greenhouse gases (GHG). Pig farming represents 9% of global livestock GHG emissions, without considering other activities of pork production process, such as feed production. Most of information about pig farms GHG emissions is based on theoretical calculations with not too much accuracy. Hence, there is a critical need to study the best sampling and analytical techniques (portable or not) that can be used to map their contribution to GHG emissions. The selection of the best analytical detection method becomes important for public policies on climate change, and in order to evaluate animal and manure handling practices to reduce GHG and to combat global warming. In this article, different techniques, which could be used to measure the emissions of GHG from livestock, are reviewed, showing the advantages and disadvantages of each technique, with special emphasis on those already used in studies about GHG from pig farms and those that allow the simultaneous determination of several species of gases. Open chambers equipped with photoacoustic multi-gas monitor have been the techniques most employed in intensive pig farms studies. Gas Chromatography coupled to different detectors has been only widely used in pig farms to monitor simultaneously several GHG species using previous sampling devices. However, there are no studies in the literature based on extensive pig farms. In these systems, micrometeorological techniques could be a promising strategy.

Application of Volatilome Analysis to the Diagnosis of Mycobacteria Infection in Livestock.

Volatile organic compounds (VOCs) are small molecular mass metabolites which compose the volatilome, whose analysis has been widely employed in different areas. This innovative approach has emerged in research as a diagnostic alternative to different diseases in human and veterinary medicine, which still present constraints regarding analytical and diagnostic sensitivity. Such is the case of the infection by mycobacteria responsible for tuberculosis and paratuberculosis in livestock. Although eradication and control programs have been partly managed with success in many countries worldwide, the often low sensitivity of the current diagnostic techniques against Mycobacterium bovis (as well as other mycobacteria from Mycobacterium tuberculosis complex) and Mycobacterium avium subsp. paratuberculosis together with other hurdles such as low mycobacteria loads in samples, a tedious process of microbiological culture, inhibition by many variables, or intermittent shedding of the mycobacteria highlight the importance of evaluating new techniques that open different options and complement the diagnostic paradigm. In this sense, volatilome analysis stands as a potential option because it fulfills part of the mycobacterial diagnosis requirements. The aim of the present review is to compile the information related to the diagnosis of tuberculosis and paratuberculosis in livestock through the analysis of VOCs by using different biological matrices. The analytical techniques used for the evaluation of VOCs are discussed focusing on the advantages and drawbacks offered compared with the routine diagnostic tools. In addition, the differences described in the literature among in vivo and in vitro assays, natural and experimental infections, and the use of specific VOCs (targeted analysis) and complete VOC pattern (non-targeted analysis) are highlighted. This review emphasizes how this methodology could be useful in the problematic diagnosis of tuberculosis and paratuberculosis in livestock and poses challenges to be addressed in future research.

Thermoregulatory and Feeding Behavior under Different Management and Heat Stress Conditions in Heifer Water Buffalo (Bubalus bubalis) in the Tropics.

In the wake of climate change and global warming, the production systems of water buffaloes (Bubalus bubalis) are receiving increasing attention in the tropics, where the silvopastoral systems can improve animal welfare and production conditions. The objective of this study was to characterize the behavior of heifer buffaloes in a silvopastoral system (SPS) with Leucaena leucocephala (600 trees/ha) and in a conventional system (CVS), under intense heat stress and moderate heat stress in Cuba. We observed nine animals, with an average weight of 167.9 kg at the beginning of the study, during the daylight period, from 6:00 to 18:00 h, at 10 min intervals, for 12 days. Activities recorded were grazing, ingestion of tree leaves, rumination, water intake, walking, lying, standing, sheltering in the shade of trees, and wallowing. Sheltering in the shade of trees and wallowing were collectively considered as thermoregulatory behavior (TB). TB was different in both systems and conditions of heat stress (p < 0.05), with 4.06 in CVS and 3.81 h in SPS in the intense heat stress period, while it was 2.91 and 1.08 h for SPS and CVS, respectively, during the moderate heat stress period. The wallowing activity showed statistically significant differences (p < 0.05) in the intense heat stress season with 1.18 and 2.35 h for SPS and CVS, respectively. Time spent on feeding behavior was highest in the SPS system (p < 0.05). Longer times of thermoregulatory and feeding behavior indicate the importance of trees in animal welfare for this species in tropical conditions, thus supporting avoided deforestation and the replanting of trees in existing production systems and landscapes.

Determination of principal ergot alkaloids in swine feeding.

BACKGROUND: Ergot alkaloids are secondary metabolites produced by fungi in the genus Claviceps. They contaminate a large variety of cereals, such as rye, triticale, wheat and barley. The ingestion of contaminated cereals might cause adverse health effects in humans and animals. In fact, pigs, cattle, sheep, and poultry are involved in sporadic outbreaks and, although there are several studies about occurrence of ergot alkaloids in grain cereals, there are scarce studies focused on compound feed. RESULTS: Twelve ergot alkaloids have been quantified in 228 feed samples intended for swine. The analytes were extracted using QuEChERS with Z-Sep+ as sorbent in the clean-up step, which reduced the matrix effect, allowing limits of quantification between 2.1 and 21.7 µg kg-1 . The analytes were subsequently quantified by ultra-high-performance liquid chromatography with tandem mass spectrometry (UHPLC-MS/MS). A total of 29 samples (12.7%) revealed contamination by at least one ergot alkaloid, and among contaminated samples, 65% were contaminated by more than one. Only 6 of 12 target ergot alkaloids showed concentrations above the limit of quantification. The concentrations for individual ergot alkaloids ranged between 5.9 µg kg-1 for ergosinine to 145.3 µg kg-1 for ergometrine (the predominant ergot alkaloid), while the total ergot alkaloid content ranged from 5.9 to 158.7 µg kg-1 . CONCLUSIONS: The occurrence of ergot alkaloids in feed samples in Spain seems to be lower than in other regions of Europe. All the samples fulfilled current recommendations of the feed industry about practical limits for ergot alkaloids in pig feeds. This suggests that the feeds are safe for pig consumption, regarding the presence of ergot alkaloids. © 2021 Society of Chemical Industry.

Selectivity of Leguminous Trees by Water Buffaloes in Semi-intensive Systems.

Water buffaloes (Bubalus bubalis) manifest different levels of selectivity for different pastures and forages. Knowledge of feed selectivity is important to facilitate the design of efficient production systems that take into account optimal animal welfare. In this study, the selectivity of nine 18-month old female water buffaloes for Leucaena leucocephala, Albizia lebbeck, Gliricidia sepium, and Moringa oleifera was evaluated. After 12 h of grazing Megathyrsus maximum, the animals were housed in individual shelters and 1.2 kg of leaves from each of the four tree species were offered to the animals simultaneously. The selectivity, measured as the intake of dry matter (DM), was highest for A. lebbeck (mean = 0.34 kgDM, SD = 0.05 kg), followed by L. leucocephala (mean = 0.30 kgDM, SD = 0.03 kg), M. oleifera (mean = 0.11 kgDM, SD = 0.05 kg), and G. sepium (mean = 0.10 kgDM, SD = 0.02 kg) (P < 0.01). The crude protein intake was highest for A. lebbeck and L. leucocephala. Notably, the less selected leaves were those of G. sepium and M. oleifera. This study suggests that the inclusion of A. lebbeck and L. leucocephala in silvopastoral systems may increase both the consumption and well-being of water buffaloes in the tropics.

Udder Morphometry and Its Relationship with Intramammary Infections and Somatic Cell Count in Serrana Goats.

The external morphological traits of the mammary gland, and their relationships with somatic cell count (SCC) and the presence of intramammary infection (IMI), were studied in 30 Serrana goats, Transmontano ecotype. Globular-shaped udders were the most predominant, with slightly separated and symmetrical halves, presenting some degree of suspension. Funnel-shaped teats were the most prevalent shape with an opening of 120° to 160° degrees. Significant differences were observed between healthy group and the coagulase negative staphylococci (CNS)-infected group for udder cleft, teat perimeter and distance between teats parameters; and between healthy group and CNS or Staphylococcus aureus groups for degree of separation, teat shape and udder shape (p < 0.05). The udder shape, symmetry, degree of suspension and degree of separation parameters showed to be different depending on SCC (p < 0.05). The udder perimeter and udder depth traits showed differences between the lowest and the middle SCC group. We concluded that bifurcated pendular udders, with vertical loose teats and located close to each other, are more likely to have IMI, and have the highest SCC. The inclusion in breeding programs of certain mammary conformation traits would not only help to improve milk production, but would also decrease the susceptibility to IMI of the herd.

Effect of Silvopastoral Systems in the Thermoregulatory and Feeding Behaviors of Water Buffaloes Under Different Conditions of Heat Stress.

Buffaloes use wallowing behavior to release excess heat in tropical conditions. The aim of this study was to evaluate the impact of silvopastoral systems in the feeding and thermoregulatory behavior of water buffaloes under moderate and intense heat stress. The behavior of water buffaloes was evaluated in two different production systems. The conventional system with Guinea grass (Megathyrsus maximus) only, and the silvopastoral system with Guinea grass and Leucaena leucocephala trees. The relation between the frequency of animal activities and the length of time the animals engaged in each activity was measured during the day time (6:00-18:00 h) by visual observations at 10-min intervals. The results obtained suggest that buffaloes use tree shade to partially supplement wallowing. Feeding behavior increased under intense heat stress in the silvopastoral system indicating that it can be a promising alternative to improve the buffaloes rearing conditions in the tropics.