RESUMEN
OBJETIVO: Este estudio estuvo dirigido a determinar cuál fue la incidencia de recurrencia bioquímica entre los pacientes sometidos a prostatectomía radical por cáncer de próstata localizado en el Hospital Nacional Edgardo Rebagliati Martins durante el periodo enero 2000-diciembre 2004 y cuáles fueron sus factores asociados. MATERIALES Y METODOS: Estudio descriptivo tipo retrospectivo, correlacional. RESULTADOS: Se estudiaron 144 pacientes. La incidencia de PSA>0.4: se presenta en un 36.1 por ciento. El PSA>0.4 y el estadio T2b final, da un p=0.00001 OR: 0.08 (0.03-0.20). PSA>0.4 y los márgenes positivos, da un p=0.00002 OR 0.23 (0.12-0.35). PSA>0.4 y Gleason de la biopsia, da un p=0.0002 OR 0.15 (0.05-0.45). PSA>0.4 y Gleason del espécimen da un p=0.0001 OR 0.05 (0.01-0.38). PSA>0.4 y PSA prequirúrgico >10, da un p=0.00002 OR 0.08 (0.02-0.27). CONCLUSION: Se encontró estadísticamente significativa con un valor de p<0.05, PSA preoperatorio >10, el estadio clínico T2c, márgenes positivos y el score de Gleason 4+3 o mayor en la pieza quirúrgica son predictores independientes de recurrencia bioquímica en pacientes con cáncer de próstata.
OBJECTIVE: This study was aimed to determine what was the incidence of biochemical recurrence in patients undergoing radical prostatectomy for localized prostate cancer in the Edgardo Rebagliati Martins National Hospital during the period January 2000-December 2004 and what were the factors associated. MATERIALS AND METHODS: A retrospective descriptive study, correlational. RESULTS: 144 patients were studied. The incidence of PSA>04: comes in a 36.1 per cent. The PSA>0.4 and T2b stage end, gives p=0.00001 OR: 0.08 (0.03 to 0.20). PSA>0.4 and positive margins, gives p=0.00002 OR 0.23 (0.12 to 0.35). PSA>0.4 and Gleason biopsy gives a p=0.0002 OR 0.15 (0.05-0.45). PSA>0.4 and specimen Gleason gives a p=0.0001 OR 0.05 (0.01 to 0.38). PSA>0.4 and preoperative PSA>10, gives p=0.00002 OR 0.08 (0.02 to 0.27). CONCLUSION: We found statistically significant with a p-value<0.05, preoperative PSA>10, clinical stage T2c, positive margins and Gleason score 4+3 or greater in the surgical specimen are independent predictors of biochemical recurrence in patients with cancer prostate.
Asunto(s)
Humanos , Masculino , Persona de Mediana Edad , Antígeno Prostático Específico , Neoplasias de la Próstata/cirugía , Prostatectomía , Recurrencia Local de Neoplasia/epidemiología , Estudios Retrospectivos , Estudios Transversales , Estudios de Casos y ControlesRESUMEN
HLA-G molecule plays an important role on immune response regulation and has been implicated on the inhibition of T and natural killer cell cytolytic function and inhibition of allogeneic T-cell proliferation. Due to its immune-modulator properties, the HLA-G gene expression has been associated with the outcome of allograft and of autoimmune, infectious, and malignant disorders. Several lines of evidence indicate that HLA-G polymorphisms at the 5'-upstream regulatory region (5' URR) and 3'-untranslated region (3' UTR) may influence the HLA-G expression levels. Because Brazilians represent one of the most heterogeneous populations in the world with the widest HLA-G coding region variability already detected among the studied populations, a high level of variability and haplotype diversity would be expected in Brazilians. On this basis, the 5' URR, coding, and 3' UTR variability were evaluated in a Brazilian series consisting of 100 healthy bone marrow donors, as well as the linkage disequilibrium pattern along the gene and the extended haplotypes encompassing several gene segment variations. The HLA-G locus seems to present six different HLA-G lineages showing functional variations mainly in nucleotides of the regulatory regions. Differences were observed at the 5' URR in positions that either coincide with or are close to transcription factor-binding sites and at the 3' UTR mainly in positions that have already been reported to influence HLA-G mRNA availability. We report several lines of evidence for balancing selection acting on the regulatory regions, which may indicate that these HLA-G lineages may be related to the differential HLA-G expression profiles.
Asunto(s)
Evolución Molecular , Regulación de la Expresión Génica/genética , Variación Genética , Antígenos HLA-G/genética , Polimorfismo Genético , Selección Genética , Secuencia de Bases , Brasil , Cartilla de ADN/genética , Haplotipos/genética , Humanos , Desequilibrio de Ligamiento , Datos de Secuencia Molecular , Análisis de Secuencia de ADNRESUMEN
The HLA-G gene displays several peculiarities that are distinct from those of classical HLA class I genes. The unique structure of the HLA-G molecule permits a restricted peptide presentation and allows the modulation of the cells of the immune system. Although polymorphic sites may potentially influence all biological functions of HLA-G, those present at the promoter and 3' untranslated regions have been particularly studied in experimental and pathological conditions. The relatively low polymorphism observed in the MHC-G coding region both in humans and apes may represent a strong selective pressure for invariance, whereas, in regulatory regions several lines of evidence support the role of balancing selection. Since HLA-G has immunomodulatory properties, the understanding of gene regulation and the role of polymorphic sites on gene function may permit an individualized approach for the future use of HLA-G for therapeutic purposes.
Asunto(s)
Antígenos HLA/genética , Antígenos de Histocompatibilidad Clase I/genética , Polimorfismo Genético , Animales , Enfermedades Autoinmunes/genética , Enfermedades Autoinmunes/inmunología , Secuencia de Bases , Evolución Molecular , Antígenos HLA/química , Antígenos HLA/inmunología , Antígenos HLA/metabolismo , Antígenos HLA-G , Antígenos de Histocompatibilidad Clase I/química , Antígenos de Histocompatibilidad Clase I/inmunología , Antígenos de Histocompatibilidad Clase I/metabolismo , Humanos , Modelos Moleculares , Datos de Secuencia Molecular , Neoplasias/genética , Neoplasias/inmunología , Inmunología del Trasplante , Virosis/etiología , Virosis/genética , Virosis/inmunologíaRESUMEN
In order to determine whether a human homolog (NRAMP1) to a murine candidate gene for resistance to mycobacteria influences susceptibility to human disease, we analyzed data from seven multicase leprosy families (84 individuals) from French Polynesia for linkage markers within the NRAMP1 gene and leprosy per se. Individual family members were typed at nine polymorphic loci within NRAMP1. In addition, three physically linked, polymorphic microsatellite markers-D2S104, D2S173 and D2S1471-were also typed. Linkage analyses were done using affected sibpair and LOD score methods employing different modes of inheritance with full and reduced penetrance. The results of this study strongly suggest that NRAMP1 is not linked to leprosy susceptibility in the French Polynesian families tested.