Asunto(s)
Araquidonato 12-Lipooxigenasa/genética , Ictiosis Lamelar/genética , Piel/patología , Animales , Modelos Animales de Enfermedad , Genes Recesivos , Humanos , Ictiosis Lamelar/metabolismo , Peroxidación de Lípido/genética , Ratones , Ratones Noqueados , Mutación/genética , Fenómenos Fisiológicos de la PielRESUMEN
Loss-of-function mutations in the lipoxygenase (LOX) genes ALOX12B and ALOXE3 are the second most common cause of autosomal recessive congenital ichthyosis. The encoded proteins, 12R-LOX and epidermal LOX-3 (eLOX-3), act in sequence to convert fatty acid substrates via R-hydroperoxides to specific epoxyalcohol derivatives and have been proposed to operate in the same metabolic pathway during epidermal barrier formation. Here, we show that eLOX-3 deficiency in mice results in early postnatal death, associated with similar but somewhat less severe barrier defects and morphological changes than reported earlier for the 12R-LOX-knockout mice. Skin lipid analysis demonstrated that the severity of barrier failure is related to the loss of covalently bound ceramides in both 12R-LOX- and eLOX-3-null mice, confirming a proposed functional linkage of the LOX pathway to ceramide processing and formation of the corneocyte lipid envelope. Furthermore, analysis of free oxygenated fatty acid metabolites revealed strongly reduced levels of hepoxilin metabolites in eLOX-3-deficient epidermis, indicating an additional function of eLOX-3 in mammalian skin as a hepoxilin synthase linked to the 12S-LOX pathway.
Asunto(s)
Araquidonato 12-Lipooxigenasa/fisiología , Epidermis/fisiología , Oxidorreductasas Intramoleculares/fisiología , Lipooxigenasa/fisiología , Animales , Araquidonato 12-Lipooxigenasa/genética , Ceramidas/análisis , Ceramidas/metabolismo , Modelos Animales de Enfermedad , Epidermis/química , Epidermis/metabolismo , Epidermis/patología , Ácidos Grasos no Esterificados/análisis , Ácidos Grasos no Esterificados/metabolismo , Ictiosis/genética , Ictiosis/metabolismo , Oxidorreductasas Intramoleculares/genética , Lípidos/análisis , Lipooxigenasa/genética , Ratones , Ratones Noqueados , Mutación , Índice de Severidad de la EnfermedadRESUMEN
In the yeast Saccharomyces cerevisiae three pathways lead to the formation of phosphatidylethanolamine (PE), namely decarboxylation of phosphatidylserine (PS) (i) by Psd1p in mitochondria, and (ii) by Psd2p in a Golgi/vacuolar compartment; and (iii) synthesis via CDP-ethanolamine pathway in the endoplasmic reticulum. To determine the contribution of these pathways to the supply of PE to peroxisomes, we subjected mutants bearing defects in the respective metabolic routes to biochemical and cell biological analysis. Despite these defects in PE formation mutants were able to grow on oleic acid indicating induction of peroxisome proliferation. Biochemical analysis revealed that PE formed through all three pathways was supplied to peroxisomes. These analyses also demonstrated that selective as well as equilibrium interorganelle flux of PE appear to be equally important for cellular homeostasis of this phospholipid. Electron microscopic inspection confirmed that defects in PE synthesis still allowed formation of peroxisomes, although these organelles from strains lacking PSD1 were significantly smaller than wild type. The fact that peroxisomes were always found in close vicinity to mitochondria, ER and lipid particles supported the view that membrane contact may play a role in lipid traffic between these organelles.
Asunto(s)
Redes y Vías Metabólicas , Peroxisomas/metabolismo , Fosfatidiletanolaminas/biosíntesis , Saccharomyces cerevisiae/metabolismo , Western Blotting , Redes y Vías Metabólicas/efectos de los fármacos , Mutación/genética , Ácido Oléico/farmacología , Tamaño de los Orgánulos/efectos de los fármacos , Peroxisomas/efectos de los fármacos , Peroxisomas/ultraestructura , Fosfolípidos/análisis , Saccharomyces cerevisiae/efectos de los fármacos , Saccharomyces cerevisiae/crecimiento & desarrollo , Saccharomyces cerevisiae/ultraestructura , Fracciones Subcelulares/efectos de los fármacos , Fracciones Subcelulares/metabolismoRESUMEN
Vps4p and Vps36p of Saccharomyces cerevisiae are involved in the transport of proteins to the vacuole via the carboxypeptidase Y pathway. We found that deletion of VPS4 and VPS36 caused impaired maturation of the vacuolar proaminopeptidase I (pAPI) via autophagy or the cytosol to vacuole targeting pathway. Supplementation with ethanolamine rescued this defect, leading to an increase of the cellular amount of phosphatidylethanolamine (PtdEtn), an enhanced level of the PtdEtn-binding autophagy protein Atg8p and a balanced rate of autophagy. We also discovered that maturation of pAPI was generally affected by PtdEtn depletion in a psd1Delta psd2Delta mutant due to reduced recruitment of Atg8p to the preautophagosomal structure. Ethanolamine supplementation provided the necessary amounts of PtdEtn for complete maturation of pAPI. Since the expression level of Atg8p was not compromised in the psd1Delta psd2Delta strain, we concluded that the amount of available PtdEtn was limiting. Thus, PtdEtn appears to be a limiting factor for the balance of the carboxypeptidase Y pathway and autophagy/the cytosol to vacuole targeting pathway in the yeast.
Asunto(s)
Autofagia/fisiología , Catepsina A/metabolismo , Fosfatidiletanolaminas/fisiología , Saccharomyces cerevisiae/fisiología , Vacuolas/enzimología , Aminopeptidasas/metabolismo , Secuencia de Bases , Western Blotting , Cartilla de ADN , Precursores de Proteínas/metabolismo , Procesamiento Proteico-Postraduccional , Saccharomyces cerevisiae/enzimologíaRESUMEN
Recently we reported a differentiation-dependent inhibition of telomerase activity in human epidermis. Consistent with this observation we found that in keratinocyte cultures calcium-induced differentiation correlates with a decline in telomerase activity. To get further support for a role of calcium in the regulation of telomerase and to elucidate the underlying molecular mechanisms we investigated the effect of calcium on telomerase in the human epidermal keratinocyte line HaCaT. Treatment with thapsigargin, which increases intracellular calcium concentrations, inhibited telomerase activity without down-regulating the expression of hTERT (human telomerase reverse transcriptase). This observation together with the fact that increasing calcium reduced telomerase activity in cell-free extracts suggests that calcium directly interacts with the telomerase complex. This interaction could be mediated by the calcium-binding protein S100A8 as indicated by its ability to mimic the inhibitory effect of calcium. S100A8-induced reduction in telomerase activity was abrogated by S100A9. The ratio of both proteins remained constant in cells treated with thapsigargin, but their interactions were altered similarly in intact cells after thapsigargin treatment and in cell-free extracts in response to calcium. We hypothesize that calcium binds to S100A8/S100A9 complexes and alters their composition, thus enabling S100A8 to interact with the telomerase complex and inhibit its activity.