Pilot-scale investigation of performance and microbial community in a novel system combining fixed and suspended activated sludge.

The conventional activated sludge (CAS) process is a widely used method for wastewater treatment due to its effectiveness and affordability. However, it can be prone to sludge abnormalities such as sludge bulking/foaming and sludge loss, which can lead to a decrease in treatment efficiency. To address these issues, a novel bag-based fixed activated sludge (BBFAS) system utilizing mesh bags to contain the sludge was developed for low carbon/nitrogen ratio wastewater treatment. Pilot-scale experiments demonstrated that the BBFAS system could successfully avoid the sludge abnormalities. Moreover, it was not affected by mass transfer resistance and exhibited significantly higher nitrogen removal efficiency, surpassing that of the CAS system by up to 78%. Additionally, the BBFAS system demonstrated comparable organic matter removal efficiency to CAS system. 16S rRNA gene high-throughput sequencing revealed that the bacterial community structure within the BBFAS system was significantly different from that of the CAS system. The bacteria associated with ammonium removal were more abundant in the BBFAS system than in the CAS system. The abundance of Nitrospira in the BBFAS could reach up to 6% and significantly higher than that in the CAS system, and they were likely responsible for both ammonia-oxidizing and nitrite-oxidizing functions. Clear stratification of microbial communities was observed from the outer to inner layers of the bag components due to the gradients of dissolved oxygen and other substrates. Overall, this study presents a promising approach for avoiding activated sludge abnormalities while maintaining high pollutant removal performance.

Large-scale comparative analysis reveals different bacterial community structures in full- and lab-scale wastewater treatment bioreactors.

The activated sludge process is widely used for biological wastewater treatment due to its low cost and high efficiency. Although numerous lab-scale bioreactor experiments have been conducted to investigate the microorganism performance and mechanisms in activated sludge, understanding the bacterial community differences between full- and lab-scale bioreactors has remained elusive. In this study, we investigated the bacterial communities in 966 activated sludge samples obtained from various bioreactors, including both full- and lab-scale ones, from 95 previous studies. Our findings reveal significant differences in the bacterial communities between full- and lab-scale bioreactors, with thousands of bacterial genera exclusive to each scale. We also identified 12 genera that are frequently abundant in full-scale bioreactors but rarely observed in lab-scale reactors. By using a machine-learning method, organic matter and temperature were determined as the primary factors affecting microbial communities in full- and lab-scale bioreactors. Additionally, transient bacterial species from other environments may also contribute to the observed bacterial community differences. Furthermore, the bacterial community differences between full- and lab-scale bioreactors were verified by comparing the results of lab-scale bioreactor experiments to full-scale bioreactor sampling. Overall, this study sheds light on the bacteria overlooked in lab-scale studies and deepens our understanding of the differences in bacterial communities between full- and lab-scale bioreactors.