RESUMEN
As well as being a significant source of environmental radiation exposure, α-particles are increasingly considered for use in targeted radiation therapy. A better understanding of α-particle induced damage at the DNA scale can be achieved by following their tracks in real-time in targeted living cells. Focused α-particle microbeams can facilitate this but, due to their low energy (up to a few MeV) and limited range, α-particles detection, delivery, and follow-up observations of radiation-induced damage remain difficult. In this study, we developed a thin Boron-doped Nano-Crystalline Diamond membrane that allows reliable single α-particles detection and single cell irradiation with negligible beam scattering. The radiation-induced responses of single 3 MeV α-particles delivered with focused microbeam are visualized in situ over thirty minutes after irradiation by the accumulation of the GFP-tagged RNF8 protein at DNA damaged sites.
Asunto(s)
Partículas alfa , Daño del ADN/efectos de la radiación , Proteínas de Unión al ADN/metabolismo , Ubiquitina-Proteína Ligasas/metabolismo , Partículas alfa/efectos adversos , Línea Celular Tumoral , Proteínas de Unión al ADN/genética , Genes Reporteros , Histonas/metabolismo , Humanos , Membranas Artificiales , Proteínas Recombinantes de Fusión/genética , Proteínas Recombinantes de Fusión/metabolismo , Imagen de Lapso de Tiempo , Ubiquitina-Proteína Ligasas/genéticaRESUMEN
For the first time, overproduction of hydroxyl radicals (HOË) induced by plasma hydrogenated detonation nanodiamonds (H-NDs) under X-ray irradiation is reported. Using coumarin (COU) as a fluorescent probe, we reveal a significant increase of 40% of the HOË production in the presence of H-NDs (6-100 µg ml-1) compared with water alone. This effect is related to the negative electron affinity of the hydrogenated nanodiamonds and illustrates the ability of H-NDs to produce reactive oxygen species probably via electron emission in water under X-ray irradiation.
RESUMEN
Small size and enhanced properties of nanoparticles (NP) are great advantages toward device miniaturization. However, adhesion is essential for the reliability of such NP layer-based devices. In this work, we present some quick tests to investigate the adhesion behavior of the whole NP layer by mimicking several applicative environments: biological buffers and cells, corrosion, and microfabrication processes. This statistic approach evaluates both adhesion and mobility respectively through particle density and layer homogeneity. We chose nanodiamonds (ND) as reference particles because they are spherical and inert and exhibit either positive or negative zeta potential for the same diameter while surfactant-free. Several deposition methods were used to prepare a wide range of ND layers with various densities and size distribution. We found some unexpected results confirming that the deposition method has to be carefully selected according to the targeted application. A selection of the suitable method(s) to prepare ND layers which are resilient in their applicative environment can be done based on these results. However, ND adhesion still remains critical in some conditions and thus requires further improvement. Most important, this study points out that NP adhesion behavior is more complex than simple particle detachment-or not-from the surface. The particles could also reorganize themselves in clusters. We evidenced, in particular, a surprising mobility driven by air/water interfaces during evaporation of water microdroplets. Further comparison with other materials would indicate if the highlighted phenomena could be extended to any nanoparticles layer.
