Optimizing the rate of phosphorus to enhance grain yield and quality in two Camelina sativa (L. ) crantz accessions / Otimizando a taxa de fósforo para aumentar o rendimento e a qualidade dos grãos em dois acessos de Camelina sativa (L. ) crantz

Camelina sativa (L.) Crantz is an emerging oil seed crop and research information on its response to different levels of phosphorous (P) fertilizer is lacking. The two years study was performed to investigate the response of C. sativa to various rates of P fertilizer. The experiments were laid out in Randomized Complete Block Design (RCBD) with factorial arrangement having four replications. The P was applied in soil at the rate of 0, 30, 40 and 60 kg ha-¹ to two C. sativa accessions namely Canadian and Australian. Soil applied phosphorus rates had significant effects on the growth, yield and quality of C. sativa and two accessions were varied to each other. Australian accession performed better in terms of quality traits and Canadian was superior in terms of seed yield. An increase in P rate improved growth, yield and quality and 60 kg P ha-¹ resulted in maximal crop growth rate (6.79), seed yield (1239 kg ha-¹), total P uptake (0.67%) and oil contents (39.8%). The regression model estimated that each increment in P rate increased the seed yield by 11.5 and 11.2 kg ha-¹ in Canadian and Australian accessions, respectively. Conclusively, increases in P rates (0 to 60 kg ha-¹) impart a positive impact on C. sativa accessions and 60 kg P ha-¹ was most effective to achieve optimum yield and profitability.

Camelina sativa (L.) Crantz é uma cultura de sementes oleaginosas emergente e faltam informações de pesquisa sobre sua resposta a diferentes níveis de fertilizante de fósforo (P). O estudo de dois anos foi realizado para investigar a resposta de C. sativa a várias doses de fertilizante P. Os experimentos foram dispostos em blocos casualizados em arranjo fatorial com quatro repetições. O P foi aplicado em solo na proporção de 0, 30, 40 e 60 kg ha-¹ em dois acessos de C. sativa, canadenses e australianos. As doses de P aplicadas no solo tiveram efeitos significativos sobre o crescimento, rendimento e qualidade de C. sativa e dois acessos foram variados entre si. O acesso australiano teve melhor desempenho em termos de características de qualidade e o canadense foi superior em termos de rendimento de sementes. O aumento na taxa de P melhorou o crescimento, rendimento e qualidade e 60 kg P ha-¹ resultou em taxa máxima de crescimento da cultura (6,79), rendimento de sementes (1239 kg ha-¹), absorção total de P (0,67%) e teor de óleo (39,8 %). O modelo de regressão estimou que cada incremento na dose de P aumentou a produtividade de sementes em 11,5 e 11,2 kg ha-¹ nos acessos canadense e australiano, respectivamente. Conclusivamente, aumentos nas doses de P (0 a 60 kg ha-¹) conferem um impacto positivo sobre os acessos de C. sativa e 60 kg P ha-¹ foram mais eficazes para atingir rendimento e lucratividade ideais.

Optimizing the rate of phosphorus to enhance grain yield and quality in two Camelina sativa (L. ) crantz accessions / Otimizando a taxa de fósforo para aumentar o rendimento e a qualidade dos grãos em dois acessos de Camelina sativa (L. ) crantz

Camelina sativa (L.) Crantz is an emerging oil seed crop and research information on its response to different levels of phosphorous (P) fertilizer is lacking. The two years study was performed to investigate the response of C. sativa to various rates of P fertilizer. The experiments were laid out in Randomized Complete Block Design (RCBD) with factorial arrangement having four replications. The P was applied in soil at the rate of 0, 30, 40 and 60 kg ha-¹ to two C. sativa accessions namely Canadian and Australian. Soil applied phosphorus rates had significant effects on the growth, yield and quality of C. sativa and two accessions were varied to each other. Australian accession performed better in terms of quality traits and Canadian was superior in terms of seed yield. An increase in P rate improved growth, yield and quality and 60 kg P ha-¹ resulted in maximal crop growth rate (6.79), seed yield (1239 kg ha-¹), total P uptake (0.67%) and oil contents (39.8%). The regression model estimated that each increment in P rate increased the seed yield by 11.5 and 11.2 kg ha-¹ in Canadian and Australian accessions, respectively. Conclusively, increases in P rates (0 to 60 kg ha-¹) impart a positive impact on C. sativa accessions and 60 kg P ha-¹ was most effective to achieve optimum yield and profitability.(AU)

Camelina sativa (L.) Crantz é uma cultura de sementes oleaginosas emergente e faltam informações de pesquisa sobre sua resposta a diferentes níveis de fertilizante de fósforo (P). O estudo de dois anos foi realizado para investigar a resposta de C. sativa a várias doses de fertilizante P. Os experimentos foram dispostos em blocos casualizados em arranjo fatorial com quatro repetições. O P foi aplicado em solo na proporção de 0, 30, 40 e 60 kg ha-¹ em dois acessos de C. sativa, canadenses e australianos. As doses de P aplicadas no solo tiveram efeitos significativos sobre o crescimento, rendimento e qualidade de C. sativa e dois acessos foram variados entre si. O acesso australiano teve melhor desempenho em termos de características de qualidade e o canadense foi superior em termos de rendimento de sementes. O aumento na taxa de P melhorou o crescimento, rendimento e qualidade e 60 kg P ha-¹ resultou em taxa máxima de crescimento da cultura (6,79), rendimento de sementes (1239 kg ha-¹), absorção total de P (0,67%) e teor de óleo (39,8 %). O modelo de regressão estimou que cada incremento na dose de P aumentou a produtividade de sementes em 11,5 e 11,2 kg ha-¹ nos acessos canadense e australiano, respectivamente. Conclusivamente, aumentos nas doses de P (0 a 60 kg ha-¹) conferem um impacto positivo sobre os acessos de C. sativa e 60 kg P ha-¹ foram mais eficazes para atingir rendimento e lucratividade ideais.(AU)

Nutrient digestibility, nitrogen excretion, and milk production of mid-lactation Jersey × Friesian cows fed diets containing different proportions of rumen-undegradable protein.

The present study was planned to test the hypothesis that feeding lactating dairy cattle with varying levels of rumen-undegradable protein (RUP) can enhance protein utilization, milk production, milk protein, and nitrogen (N) excretion. Forty mid-lactating crossbred (Jersey × Friesian) cattle were randomly divided into four groups. Four treatment diets were formulated to contain 30%, 40%, 50%, and 60% RUP of crude protein. Dry matter (DM) and crude protein intakes were significantly reduced with increasing dietary RUP levels. Crude protein digestibility increased linearly with incremental increases in dietary RUP levels. Cattle fed 60% RUP showed a linear decrease in N intake compared to that in the other groups. A linear decrease in urinary N and linear increases in net N, milk N, and N-use efficiency were observed with increasing dietary RUP levels. Actual milk, energy-corrected milk, and 4% fat-corrected milk yields (kg/day) increased linearly with an increasing degradability of protein. However, milk protein, solids not fat and total solids, as well as the yields of protein, fat, and lactose, showed significant increases with increased RUP supplementation. Collectively, the results indicate that formulating dairy cow diets to contain 60% RUP results in better lactating performance and N-use efficiency and lower N excretion.

Quorum Sensing Interfering Strategies and Their Implications in the Management of Biofilm-Associated Bacterial Infections

Abstract The bacterial species employ various types of molecular communication systems recognized as quorum sensing for the synchronization of differential gene expression to regulate virulence traits and biofilm formation. A variety of quorum sensing inhibitors; molecules that interfere with quorum sensing among bacteria have been examined which can block the action of autoinducers. Moreover, the studies have scrutinized various enzymes for their quorum quenching activity resulting in the degradation of signaling molecules or blocking of gene expression. So far, the studies have found that these approaches are not only capable to reduce the pathogenicity and biofilm formation but also resulted in increased bacterial susceptibility to antibiotics and bacteriophages. The effectiveness of these strategies has been validated in different animal models and it seems that these practices will be transformed in near future to develop the medical devices including catheters, implants, and dressings for the prevention of bacterial infections. Although many of these approaches are still in the research stage, the increasing library of quorum quenching molecules and enzymes will open innovative perspectives for the development of antibacterial approaches which will extend the therapeutic arsenal against the pathogenic bacterial species.

Sarcocystosis in South American camelids: The state of play revisited.

Members of the genus Sarcocystis (Apicomplexa: Sarcocystidae) are intracellular protozoan parasites that infect a wide range of domestic and wild animals, resulting in economic losses in production animals worldwide. Sarcocystis spp. have indirect life-cycles where canids and felids serve as main definitive hosts while a range of domestic and wild animals serve as intermediate hosts, including South American camelids (SACs) such as alpacas, llamas and guanacos. These animals primarily occur in South American countries on Andean, elevated plains but in recent years, alpacas and llamas have become emerging animal industries in other parts of the world such as Australia, Europe and the USA due to their high-quality fiber, meat and hides. For instance, alpaca meat is becoming popular in many parts of the world due to its lower cholesterol content than other red meat, thereby it has the potential of a valuable product for both local and international markets. However, SAC meat can be degraded and/or even condemned due to the presence of macroscopic sarcocysts in skeletal muscles, leading to significant economic losses to farmers. The infection is generally asymptomatic, though highly pathogenic or even fatal Sarcocystis infections have also been reported in alpacas and llamas. Despite the economic importance of sarcocystosis in SACs, little is known about the life-cycle of parasites involved, disease transmission, epidemiology, pathogenesis, diagnosis, control and public health significance. This review article provides an in-depth analysis of the existing knowledge on the taxonomy, epidemiology, clinicopathology and diagnosis of Sarcocystis in SACs, highlights knowledge gaps and proposes future areas of research that could contribute to our better understanding of sarcocystosis in these animals.

Assessment of flatulence causing agents in Chickpea (Cicer arietinum L.) and their possible removal

Abstract Flatulence and fullness of stomach is one of the most common problem associated with chickpea primary due to presence of some oligosaccharides and phenols. In this investigation Desi and Kabuli varieties were compared for these oligosaccharides and phenolic compounds. Furthermore, the effect of different processing and cooking methods such as soaking, cooking and germination in the reduction of these antiphysiological factors were are also studies. Maximum tannic acid (0.90 ± 0.20%) was observed in Parbat and C-44 while minimum (0.60 ± 0.04%) in Karak-2. Stachyose contents ranged between 1.10 ± 0.05 (Karak-3) to 1.42 ± 0.02% (Parbat) while raffinose was 0.63 ± 0.05(Karak-3) to 0.81 ± 0.02% (Dasht). The highest tannic acid content was reduced up to 50% in C-44 by cooking of 72 hours germinated seeds. Stachyose and raffinose contents were completely removed after 72 hours germination. Present studies revealed that cooking after germination is the most effective method to reduce the anti-nutritional factors of chickpea. Individually, soaking and cooking also contributed to the loss of the same factors but to a lesser extent.

Multiple drug resistance patterns in various phylogenetic groups of uropathogenic E. coli isolated from Faisalabad region of Pakistan

The objective of this work was the phylogenetic characterization of local clinical isolates of uropathogenic E. coli with respect to drug resistance. A total of 59 uropathogenic E. coli responsible for community acquired urinary tract infections were included in this study. A triplex PCR was employed to segregate each isolate into four different phylogenetic groups (A, B1, B2 and D). Drug resistance was evaluated by disc diffusion method. The drugs used were ampicillin, aztreonam, cefixime, cefoperazone, ceftriaxone, cephradine among â-lactam group; amikacin, gentamicin, and streptomycin among aminoglycosides; nalidixic acid and ciprofloxacin from quinolones; trimethoprim-sulfomethoxazole, and tetracycline. Among 59 uropathogenic E. coli isolates majority belonged to phylogenetic group B2 (50 percent) where as 19 percent each belonged to groups A and B1, and 12 percent to group D. All the isolates were multiple drug resistant (MDR). Most effective drugs against Group A, B1, and B2 were gentamicin, amikacin and cefixime; ceftriaxone and quinolones; and ceftriaxone and amikacin, respectively. Group D isolates were found to be highly resistant to all drugs. Our results have shown emergence of MDR isolates among uropathogenic E. coli with dominance of phylogenetic group B2. However, it was found that group D isolates were though less frequent, more drug resistant as compared with group B2. Groups A and B1 were relatively uncommon. Amikacin, ceftriaxone and gentamicin were the most effective drugs in general.

QTL mapping for physiology, yield and plant architecture traits in cotton (Gossypium hirsutum L. ) grown under well-watered versus water-stress conditions

Increasing scarcity of irrigation water is a major threat to sustainable production of cotton (Gossypium hirsutum L.). Identifying genomic regions contributing to abiotic stress tolerance will help develop cotton cultivars suitable for water-limited regions through molecular marker-assisted breeding. A molecular mapping F2 population was derived from an intraspecific cross of the drought sensitive G. hirsutum cv. FH-901 and drought tolerant G. hirsutum cv. RH-510. Field data were recorded on physiological traits (osmotic potential and osmotic adjustment); yield and its component traits (seedcotton yield, number of bolls/plant and boll weight); and plant architecture traits (plant height and number of nodes per plant) for F2, F2:3 and F2:4 generations under well-watered versus water-limited growth conditions. The two parents were surveyed for polymorphism using 6500 SSR primer pairs. Joinmap3.0 software was used to construct linkage map with 64 polymorphic markers and it resulted into 35 markers mapped on 12 linkage groups. QTL analysis was performed by composite interval mapping (CIM) using QTL Cartographer2.5 software. In total, 7 QTLs (osmotic potential 2, osmotic adjustment 1, seedcotton yield 1, number of bolls/plant 1, boll weight 1 and plant height 1) were identified. There were three QTLs (qtlOP-2, qtlOA-1, and qtlPH-1) detected only in water-limited conditions. Two QTLs (qtlSC-1 and qtlBW-1) were detected for relative values. Two QTLs (qtlOP-1 and qtlBN-1) were detected for well-watered treatment. Significant QTLs detected in this study can be employed in MAS for molecular breeding programs aiming at developing drought tolerant cotton cultivars.

Multiple drug resistance patterns in various phylogenetic groups of uropathogenic E.coli isolated from Faisalabad region of Pakistan.

The objective of this work was the phylogenetic characterization of local clinical isolates of uropathogenic E. coli with respect to drug resistance. A total of 59 uropathogenic E. coli responsible for community acquired urinary tract infections were included in this study. A triplex PCR was employed to segregate each isolate into four different phylogenetic groups (A, B1, B2 and D). Drug resistance was evaluated by disc diffusion method. The drugs used were ampicillin, aztreonam, cefixime, cefoperazone, ceftriaxone, cephradine among ß-lactam group; amikacin, gentamicin, and streptomycin among aminoglycosides; nalidixic acid and ciprofloxacin from quinolones; trimethoprim-sulfomethoxazole, and tetracycline. Among 59 uropathogenic E. coli isolates majority belonged to phylogenetic group B2 (50%) where as 19% each belonged to groups A and B1, and 12% to group D. All the isolates were multiple drug resistant (MDR). Most effective drugs against Group A, B1, and B2 were gentamicin, amikacin and cefixime; ceftriaxone and quinolones; and ceftriaxone and amikacin, respectively. Group D isolates were found to be highly resistant to all drugs. Our results have shown emergence of MDR isolates among uropathogenic E. coli with dominance of phylogenetic group B2. However, it was found that group D isolates were though less frequent, more drug resistant as compared with group B2. Groups A and B1 were relatively uncommon. Amikacin, ceftriaxone and gentamicin were the most effective drugs in general.

Multiple drug resistance patterns in various phylogenetic groups of uropathogenic E.coli isolated from Faisalabad region of Pakistan

The objective of this work was the phylogenetic characterization of local clinical isolates of uropathogenic E. coli with respect to drug resistance. A total of 59 uropathogenic E. coli responsible for community acquired urinary tract infections were included in this study. A triplex PCR was employed to segregate each isolate into four different phylogenetic groups (A, B1, B2 and D). Drug resistance was evaluated by disc diffusion method. The drugs used were ampicillin, aztreonam, cefixime, cefoperazone, ceftriaxone, cephradine among -lactam group; amikacin, gentamicin, and streptomycin among aminoglycosides; nalidixic acid and ciprofloxacin from quinolones; trimethoprim-sulfomethoxazole, and tetracycline. Among 59 uropathogenic E. coli isolates majority belonged to phylogenetic group B2 (50%) where as 19% each belonged to groups A and B1, and 12% to group D. All the isolates were multiple drug resistant (MDR). Most effective drugs against Group A, B1, and B2 were gentamicin, amikacin and cefixime; ceftriaxone and quinolones; and ceftriaxone and amikacin, respectively. Group D isolates were found to be highly resistant to all drugs. Our results have shown emergence of MDR isolates among uropathogenic E. coli with dominance of phylogenetic group B2. However, it was found that group D isolates were though less frequent, more drug resistant as compared with group B2. Groups A and B1 were relatively uncommon. Amikacin, ceftriaxone and gentamicin were the most effective drugs in general.