Plastic pollution has become a global problem since the extensive use of plastic in industries such as packaging, electronics, manufacturing and construction, healthcare, transportation, and others. This has resulted in an environmental burden that is continually growing, which has inspired many scientists as well as environmentalists to come up with creative solutions to deal with this problem. Numerous studies have been reviewed to determine practical, affordable, and environmentally friendly solutions to regulate plastic waste by leveraging microbes' innate abilities to naturally decompose polymers. Enzymatic breakdown of plastics has been proposed to serve this goal since the discovery of enzymes from microbial sources that truly interact with plastic in its naturalistic environment and because it is a much faster and more effective method than others. The scope of diverse microbes and associated enzymes in polymer breakdown is highlighted in the current review. The use of co-cultures or microbial consortium-based techniques for the improved breakdown of plastic products and the generation of high-value end products that may be utilized as prototypes of bioenergy sources is highlighted. The review also offers a thorough overview of the developments in the microbiological and enzymatic biological degradation of plastics, as well as several elements that impact this process for the survival of our planet.
Introduction: The area of "Green Synthesis of Nano-medicine," as compared to its synthetic counterparts, is a relatively safer research technology for various biomedical applications, including identification, therapeutic application, and prevention of pathological conditions, pain control, safety, and development of human wellness. The present study explored the synthesis and characterization of AgNPs using the ethanolic extract of Piper cubeba fruit as a reducing and stabilizing agent and its potential as an enzyme inhibitory agent. Urease inhibitors are helpful against many severe diseases, including gastric ulcers induced by Helicobacter pylori. Method: The fruits of the Piper cubeba plant were taken and ground to a fine powder. Plant material was added to 500 ml ethanol, and the mixture was filtered. The solvent of the filtrate was evaporated, and a thick, gummy extract was obtained and stored at 4°C in the refrigerator. AgNPs were green synthesized from solutions of AgNO3 using the P. cubeba extract, which was indicated by a change in the color from light brown to deep brown. The synthesized AgNPs were characterized via Ultraviolet-visible (UV-Vis) spectroscopy, Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), and scanning electron microscopy (SEM). Results and Discussion: Analysis showed the reduction of Ag+ to Ag0 at room temperature (25°C), and the average particle size of AgNPs was in the range of 40-80 nm. Consequently, the synthesized AgNPs were evaluated for their anti-urease activity. The maximum urease inhibition of the Piper cubeba ethanolic extract was 88.5% at 5 mg conc., and of derived nanoparticles was 78.6% at 0.05 mg conc. The results were nearly similar to the control drug, i.e., thiourea (0.5 and 0.6 mM conc., respectively). Conclusion: The study concluded that the P. cubeba extract, as well as its green-derived AgNPs, might prove to be a better and safer substitute for their enzyme inhibitory potential in emerging medicine and novel drug delivery techniques to improve and maintain human health.
