The cadmium tolerance and bioaccumulation mechanism of Tetratostichococcus sp. P1: insight from transcriptomics analysis.

Cadmium (Cd) has become a severe issue in relatively low concentration and attracts expert attention due to its toxicity, accumulation, and biomagnification in living organisms. Cd does not have a biological role and causes serious health issues. Therefore, Cd pollutants should be reduced and removed from the environment. Microalgae have great potential for Cd absorption for waste treatment since they are more environmentally friendly than existing treatment methods and have strong metal sorption selectivity. This study evaluated the tolerance and ability of the microalga Tetratostichococcus sp. P1 to remove Cd ions under acidic conditions and reveal mechanisms based on transcriptomics analysis. The results showed that Tetratostichococcus sp. P1 had a high Cd tolerance that survived under the presence of Cd up to 100 µM, and IC50, the half-maximal inhibitory concentration value, was 57.0 µM, calculated from the change in growth rate based on the chlorophyll content. Long-term Cd exposure affected the algal morphology and photosynthetic pigments of the alga. Tetratostichococcus sp. P1 removed Cd with a maximum uptake of 1.55 mg g-1 dry weight. Transcriptomic analysis revealed the upregulation of the expression of genes related to metal binding, such as metallothionein. Group A, Group B transporters and glutathione, were also found upregulated. While the downregulation of the genes were related to photosynthesis, mitochondria electron transport, ABC-2 transporter, polysaccharide metabolic process, and cell division. This research is the first study on heavy metal bioremediation using Tetratostichococcus sp. P1 and provides a new potential microalga strain for heavy metal removal in wastewater.[Figure: see text]Abbreviations:BP: Biological process; bZIP: Basic Leucine Zipper; CC: Cellular component; ccc1: Ca (II)-sensitive cross complementary 1; Cd: Cadmium; CDF: Cation diffusion facilitator; Chl: Chlorophyll; CTR: Cu TRansporter families; DAGs: Directed acyclic graphs; DEGs: Differentially expressed genes; DVR: Divinyl chlorophyllide, an 8-vinyl-reductase; FPN: FerroportinN; FTIR: Fourier transform infrared; FTR: Fe TRansporter; GO: Gene Ontology; IC50: Growth half maximal inhibitory concentration; ICP: Inductively coupled plasma; MF: molecular function; NRAMPs: Natural resistance-associated aacrophage proteins; OD: Optical density; RPKM: Reads Per Kilobase of Exon Per Million Reads Mapped; VIT1: Vacuolar iron transporter 1 families; ZIPs: Zrt-, Irt-like proteins.

Future trends and patterns in leachate biological treatment research from a bibliometric perspective.

Leachate has become a great deal of concern due to its complex properties which are primarily caused by the high concentrations of organics and ammonia. Thus, proper leachate treatment is required prior to its discharge. Leachate can be treated in various ways, and biological treatment is one of the approaches. This treatment has been shown to be both effective and cost-efficient while offering the possibility of resource recovery in the form of bioenergy. In this study, the underlying patterns in publications related to leachate biological treatment were uncovered through bibliometric analysis. This study also lays the groundwork for a deeper understanding of the past, current, and future trends of the leachate biological treatment. Research publications from 1974 to 2021 were retrieved from the Scopus database, and it was identified that 2013 articles were published in the span of 47 years. From the analyzed publications, China played a leading role in publishing leachate biological treatment research articles as well as having the most productive institutions and authors. Meanwhile, the USA was found to be the most active country in initiating international collaborations with 33 countries. The research hotspots were also successfully identified using keyword co-occurrences analysis. Anaerobic digestion and constructed wetland were revealed to be the research hotspots. The critical role of biological treatment in removing nitrogen from leachate was also highlighted. Besides, numerous research gaps were identified in the application of aerobic granular sludge (AGS) for leachate treatment. This can be a potential area for research in the future. Finally, future research should be encouraged to focus on the use of sustainable treatment systems in which energy recovery in the form of biogases is promoted.