[The effects of Ketac Molar Aplicap glass-ionomer material on growth of cariogenic bacteria contained in the dental plaque]. / Wplyw materialu szklo-jonomerowego Ketac Molar Aplicap na wzrost bakterii próchnicotwórczych plytki nazebnej.

INTRODUCTION: In the aging population, the prevalence of root caries has been observed, which is a characteristic feature of the elderly people. The most important element used in caries prevention is fluoride, which is derived from the air, diet or fluoride-containing preparations and materials, e.g. glass-ionomer restorations. The aim of the study was to evaluate the effect of Ketac Molar Aplicap glass-ionomer on the growth of Lactobacillus sp. bacteria, one of the species most frequently found in the carietic focus of the tooth root. METHODS: The study was carried out in 15 patients with good oral hygiene, in whom 35 fillings from Ketac Molar Aplicap conventional glass-ionomer material were performed. After 6 months, three-day dental plaque from these fillings and from the tooth enamel of the control group was examined. RESULTS: No statistically significant differences (p = 0.554) in the amounts of Lactobacillus sp. between the study and control group were revealed. CONCLUSIONS: Lack of inhibiting effect of glass-ionomer material on the growth of the dental plaque with Lactobacillus sp. after the time of observation is implied.

[Influence of topical fluoridation of glass ionomer cements on inhibitory activity on cariogenic bacteria]. / Wplyw fluorkowania kontaktowego materialów szklojonomerowych na wlasciwosci hamowania przez nie wzrostu bakterii plytki nazebnej.

Glass ionomer cements are important options in restorative and preventive dentistry due to their adhesion to the tooth surface and fluoride release, which can decrease the risk of recurrent caries. The aim of this study was to define, in vivo, the influence of the topical use of fluoride gel on dental plaque bacteria growing on the glass ionomer cement. Fifteen patients were included into this study. Thirty five class V restorations from the glass ionomer cement (Ketac Molar Aplicap, ESPE Germany) were placed in the patient's one half of the lower jaw. The sound enamel of other side of the lower jaw was treated as a control. After 6 month 72 old dental plaque was collected from the surfaces of restorations and the surfaces of the sound enamel. Total amount of 30 dental plaque samples were investigated according to the previously described method (17). In dental plaque samples the amount of Streptococcus mutans was calculated at the Department of Microbiology, Medical University of Lódz. Next the topical application of fluoride gel (Fluormex) was performed on the surfaces of glass ionomer (Ketac Molar) fillings and the sound enamel. The patients were asked not to clean the teeth for 72 h. After this time the dental plaque was again collected from the surfaces of restorations and sound enamel. Statistical analysis of collected data was accomplished and showed no statistically significant differences in the amount of Streptococcus mutans both on Ketac Molar and the enamel before and after the topical use of fluoride gel. It was concluded that the topical fluoridation of glass ionomer cement did not affect Streptococcus mutans growing in dental plaque.

[Influence of the fluoride releasing dental materials on the bacterial flora of dental plaque]. / Wplyw materialów uwalniajacych fluor na sklad plytki nazebnej.

The assessment of influence of silver-free, fluor releasing dental materials on dental plaque bacteria quantity. 17 patients were included into the study. 51 restorations were placed following manufacturers recommendations. Following materials were used: conventional glassionomer Ketac-Molar ESPE, resin modified glassionomer Fuji II LC GC and fluor containing composite Charisma Heraeus Kulzer Class V restorations were placed in following teeth of upper and lower jaw: canines, first bicuspids, second bicuspids. Sound enamel was a control. After 10 weeks the 72 hours old dental plaque was collected from surface of restorations and control using sterile probe. Total amount of 68 dental plaques were investigated. Each plaque was placed on scaled and sterile aluminum foil. The moist weight of dental plaque was scaled. Dental plaque was moved into 7 ml 0.85% NaCl solution reduced by cystein chlorine hydrogen and disintegrated by ultrasounds (power:100 Watt, wave amplitude: 5 micorm). The suspension of dental plaque was serially diluted from 10(-4) to 10(-5) in sterile 0,85% NaCl solution, and seeded with amount of 0.1 ml on appropriate base. In dental plaque trials the amount of cariogenic bacteria was calculated--Streptococcus mutans, Streptococcus, Lactobacillus, Veillonella and Neisseria, and also total amount of aerobic and anaerobic bacteria was measured. Microbiologic studies were performed in Institute of Microbiology, Medical University, Lódz. Statistical analysis of collected data was accomplished. In 72 hours old dental plaques collected from the surfaces of Ketac -Molar, Fuji II LC, Charisma after 10 weeks since being placed into the class V cavity, results show no statistically significant differences in the amount of Streptococcus mutans, Streptococcus spp., Lactobacillus spp., Veillonella spp., Neisseria spp, in total amount of aerobic and anaerobic bacteria and in the quantity proportion of Streptococcus mutans versus Streptococcus spp. in comparison with control trail. Results show no statistically significant differences in the amount of listed above bacteria and in the proportion of Streptococcus mutans versus Streptococcus spp. in 72 hours old dental plaques collected from surfaces of investigated restorative materials.

[Comparison of bacterial flora in the oral cavity of children and adults]. / Porównanie flory bakteryjnej jamy ustnej u dzieci i u osób doroslych.

The aim of the study was to assess levels of occurrence and number of aerobic bacteria hemolysing and non-hemolysing, anaerobic bacteria, streptococci hemolysing and non-hemolysing, staphylococci, and bacteria responsible for tooth decay (Streptococcus mutans and Lactobacillus spp.) on oral cavity in children and adults. The results obtained indicate the difference of the level of occurrence of, aerobic bacteria hemolysing and non-hemolysing, anaerobic bacteria, streptococci hemolysing and non-hemolysing, staphylococci, and bacteria responsible for tooth decay (Streptococcus mutans and Lactobacillus spp.) was not statistically significant in either group. The counts and average values of the counts for aerobic bacteria non-hemolysing, anaerobic bacteria, streptococci hemolysing and non-hemolysing and Streptococcus mutans turned out to be statistically significantly larger in adults than in children. However for aerobic bacteria hemolysing, staphylococci and Lactobacillus spp. the difference of the counts was not statistically significant in either group.