[Progesterone receptors in the prognosis after therapy of pre- and post-menopausal patients with squamous intraepithelial lesions and cancer of the uterine cervix]. / Receptores a progesterona en el pronóstico de la terapia en pacientes pre y posmenopáusicas con lesiones escamosas intraepiteliales y por cáncer cérvico uterino.

The objective of this study was to know if there is a correlation in contents progesterone receptors (RPg) in biopsies from patients pre and postmenopausal, with normal cervix, Low-Grade squamous intraepithelial lesions (LGESIL) and High-Grade (HGSIL), and invasive cancer. Sixty three patients with abnormal cytology; colposcopy was carried out and two biopsies were taken from the suspicious lesion, they were sent for histopathological study, and for RPg; both studies were correlated later. Both pre and postmenopausal with LGSIL, the RPg the mean values were 16.81 fmol/mg, protein, and values negative, respectively. With regard to HGSIL, the RPg content, the mean values was 20.31 fmol/mg, protein in pre-menopausal patients, whereas it was 3.8 fmol/mg, protein in postmenopausal patients. It was seen that RPg concentration is higher in invasive cancer than to LGSIL and HGSIL patients in pre and postmenopausal. It is concluded that this study on quantification to RPg receptor level measurement may well be to select poor prognostic groups of patients for inclusion in the management of squamous intraepithelial lesions and invasive cancer.

[Reductive effect of the sesquiterpenic lactone "Helenalin " and its metallic derivatives in the formation of the estradiol receptor complex in breast tumor tissue]. / Efecto reductor de la lactona sesquiterpénica "Helenalina" y sus derivados metálicos en la formación del complejo estradiol receptor en tejido tumoral mamario.

Chemotherapy for systemic therapy in breast cancer has been widely used, and has been supported by many varied compounds with different origins and different compositions. Nevertheless, all of them produce several side adverse effects which must be taken into account. For this reason we must study new possibilities in which the administered drug acts selectively on the tumour cell without injuring healthy tissue. For studying its effect, a gamma lactone called "Helenaline" and its metalic derivates He-Co, He-Hg and He-Cu were studied, which chemical composition allows them to react with -SH residues present in the tumour cell receptors, which when interspaced by a previous reaction, could modify its structural composition and finally its affinity by the hormone. The inhibition effect for formation of estradiol-receptor complex in breast tumour tissue using Helenaline 12 n M and 126 n M was studied, obtaining 14% and 56% inhibition effect respectively. When He-Co, He-Hg and He-Cu effect was studied, this effect was raised obtaining 11%, 10.5% and 60% with 12 nM and 44.5%, 74.4% and 86% with 126 nM respectively.

[Infiltrating duct breast carcinoma: the role of estradiol and progesterone receptors]. / Carcinoma mamario ductal infiltrante. Papel de los receptores a estradiol y progesterona.

The content of receptors to estradiol (ER) and progesterone (PgR) was studied in hormone-dependent tumours from pre- and postmenopausal patients with an infiltrating ductal type of mammary cancer in clinical stage III. The receptors concentration was correlated with the response endocrine treatment. (ER) and (PgR) demonstrated values in the ranges 11-275 and 11-76 femtomoles/mg protein respectively. According to the type of receptor found, four different groups were found, the group (ER+) (PgR+) was found in 25% and 26% of the pre- and postmenopausal patients respectively, 31% and 27% demonstrated the group (ER+) (PgR-). The combination (ER-) (PgR+) was present in 21% of premenopausal women and 16% in the postmenopausal conditions. 23% and 31 of the pre- and postmenopausal women presented (ER-) (PgR-) respectively. Patients, to whom tamoxifen was administered, were those who demonstrated the following receptor groups: (ER+) (PgR+), (ER+) (PgR-) and (ER-) (PgR+). Following therapeutic treatment those surviving without tumoural activity, were those who demonstrated higher estrogen receptor values. The concentration of (ER) was observed to be decreased by 29% in patients who presented distant metastasis and in the cases with local metastasis, (ER) showed at 54% decrease with respect to (ER) values from symptomatic patients. The concentration of (PgR) remained constant in all the aforementioned groups.

[Estradiol, progesterone, and dexamethasone receptors in infiltrating ductal carcinoma]. / Receptores a estradiol, progesterona y dexametazona en carcinoma ductal infiltrante.

The concentration of receptor to estradiol (E-R) progesterone (Pg-R) and dexamethasone (G-R) was studied, by the dextran coated charcoal method in the cytosol of the mammary gland tissue from patients with infiltrating ductal carcinoma. The study was done in one hundred cases. 45% of these patients were postmenopausic and 55% premenopausic, with average ages of 66 and 37 years respectively. Their first pregnancy occurred on average at 24 years and they had 3-4 gestations, 45% breast-fed for a minimum period of six months and menarche at 13 years. The specific union found in the tumours was considered "receptor positive" were in the range of 10 to 181 femtomoles/mg. The "Receptor negative" tumour was considered to have a specific union less than 10 femtomoles/mg protein. On using E-R as a marker to predict the hormonal treatment it was found in this study that 56% of these patients demonstrated R-E+ and was absent in 44%. Simultaneous determination of E-R and Pg-R, raised the number of patients presenting specific receptors to 70% and on determining E-R, Pg-R and G-R, in the same tumours as markers of hormone dependence, the number of patients that presented them, increased to 77%. Finally 23% of these patients did not contain E-R, Pg-R or G-R. When the values of R-E+ were grouped according to the menstrual state of the patient, it was found that the high values of R-E+ corresponded to the postmenopausic patients. No relation was found between the concentration of R-Pg+ and R-G+ and the patient's menstrual state.