Interaction of titanium dioxide nanoparticles with PVC-microplastics and chromium counteracts oxidative injuries in Trachyspermum ammi L. by modulating antioxidants and gene expression.

The emergence of polyvinyl chloride (PVC) microplastics (MPs) as pollutants in agricultural soils is increasingly alarming, presenting significant toxic threats to soil ecosystems. Ajwain (Trachyspermum ammi L.), a plant of significant medicinal and culinary value, is increasingly subjected to environmental stressors that threaten its growth and productivity. This situation is particularly acute given the well-documented toxicity of chromium (Cr), which has been shown to adversely affect plant biomass and escalate risks to the productivity of such economically and therapeutically important species. The present study was conducted to investigate the individual effects of different levels of PVC-MPs (0, 2, and 4 mg L-1) and Cr (0, 150, and 300 mg kg-1) on various aspects of plant growth. Specifically, we examined growth and biomass, photosynthetic pigments, gas exchange attributes, oxidative stress responses, antioxidant compound activity (both enzymatic and nonenzymatic), gene expression, sugar content, nutritional status, organic acid exudation, and Cr accumulation in different parts of Ajwain (Trachyspermum ammi L.) seedlings, which were also exposed to varying levels of titanium dioxide (TiO2) nanoparticles (NPs) (0, 25, and 50 µg mL-1). Results from the present study showed that the increasing levels of Cr and PVC-MPs in soils significantly decreased plant growth and biomass, photosynthetic pigments, gas exchange attributes, sugars, and nutritional contents from the roots and shoots of the plants. Conversely, increasing levels of Cr and PVC-MPs in the soil increased oxidative stress indicators in term of malondialdehyde, hydrogen peroxide, and electrolyte leakage, and also increased organic acid exudation pattern in the roots of T. ammi seedlings. Interestingly, the application of TiO2-NPs counteracted the toxicity of Cr and PVC-MPs in T. ammi seedlings, leading to greater growth and biomass. This protective effect is facilitated by the NPs' ability to sequester reactive oxygen species, thereby reducing oxidative stress and lowering Cr concentrations in both the roots and shoots of the plants. Our research findings indicated that the application of TiO2-NPs has been shown to enhance the resilience of T. ammi seedlings to Cr and PVC-MPs toxicity, leading to not only improved biomass but also a healthier physiological state of the plants. This was demonstrated by a more balanced exudation of organic acids, which is a critical response mechanism to metal stress.

Liquid fertilizers produced by microwave-assisted acid hydrolysis of livestock and poultry wastes and their effects on hot pepper cultivation.

Liquid fertilizers (LFs) produced by microwave-assisted acid hydrolysis of livestock and poultry wastes were applied to potted hot pepper (Capsicum annuum L.) to evaluate their potential to be used as amino acid LFs. A preliminary experiment was conducted to determine the optimum acid-hydrolysis conditions for producing LFs from a mixture of pig hair and faeces (P) and another mixture of chicken feathers and faeces (C). Two LFs were produced under the optimum acid-hydrolysis conditions (acidification by sulphuric acid (7.5 mol L-1) in a microwave (200 W) for 90 minutes), and a commercial amino acid LF (Guo Guang (GG)) was used for comparison. P, C and GG fertilizers were tested in potted hot pepper cultivation at two doses, whereas no fertilizer application served as the control (CK). P and C fertilizers significantly increased the fruit yield compared with GG fertilizer, particularly at the higher dose. Moreover, the treatments improved the fruit vitamin C and soluble sugar contents in the order of C > P > GG compared with CK. These results could be attributed to more types of amino acids in C fertilizer than in P and GG fertilizers. The results also indicated that the prepared fertilizers could significantly increase the shoot and root dry weight, soil available nitrogen and phosphorus contents and nitrogen, phosphorus, and potassium (NPK) uptake by plants compared with CK. In conclusion, microwave-assisted acid hydrolysis could effectively convert unusable wastes into valuable fertilizers comparable or even superior to commercial fertilizers.

Alleviation of cadmium toxicity in pea (Pisum sativum L.) through Zn-Lys supplementation and its effects on growth and antioxidant defense.

Cadmium significantly impacts plant growth and productivity by disrupting physiological, biochemical, and oxidative defenses, leading to severe damage. The application of Zn-Lys improves plant growth while reducing the stress caused by heavy metals on plants. By focusing on cadmium stress and potential of Zn-Lys on pea, we conducted a pot-based study, organized under completely randomized block design CRD-factorial at the Botanical Garden of Government College University, Faisalabad. Both pea cultivars were grown in several concentrations of cadmium @ 0, 50 and 100 µM, and Zn-Lys were exogenously applied @ 0 mg/L and 10 mg/L with three replicates for each treatment. Cd-toxicity potentially reduces plant growth, chlorophyll contents, osmoprotectants, and anthocyanin content; however, an increase in MDA, H2O2 initiation, enzymatic antioxidant activities as well as phenolic, flavonoid, proline was observed. Remarkably, exogenously applied Zn-Lys significantly enhanced the plant growth, biomass, photosynthetic attributes, osmoprotectants, and anthocyanin contents, while further increase in enzymatic antioxidant activities, total phenolic, flavonoid, and proline contents were noticed. However, application of Zn-Lys instigated a remarkable decrease in levels of MDA and H2O2. It can be suggested with recommendation to check the potential of Zn-Lys on plants under cadmium-based toxic soil.

Emerging trends in wastewater treatment: Addressing microorganic pollutants and environmental impacts.

This review focuses on the challenges and advances associated with the treatment and management of microorganic pollutants, encompassing pesticides, industrial chemicals, and persistent organic pollutants (POPs) in the environment. The translocation of these contaminants across multiple media, particularly through atmospheric transport, emphasizes their pervasive nature and the subsequent ecological risks. The urgency to develop cost-effective remediation strategies for emerging organic contaminants is paramount. As such, wastewater-based epidemiology and the increasing concern over estrogenicity are explored. By incorporating conventional and innovative wastewater treatment techniques, this article highlights the integration of environmental management strategies, analytical methodologies, and the importance of renewable energy in waste treatment. The primary objective is to provide a comprehensive perspective on the current scenario, imminent threats, and future directions in mitigating the effects of these pollutants on the environment. Furthermore, the review underscores the need for international collaboration in developing standardized guidelines and policies for monitoring and controlling these microorganic pollutants. It advocates for increased investment in research and development of advanced materials and technologies that can efficiently remove or neutralize these contaminants, thereby safeguarding environmental health and promoting sustainable practice.

Combined exposure of PVC-microplastic and mercury chloride (HgCl2) in sorghum (Pennisetum glaucum L.) when its seeds are primed titanium dioxide nanoparticles (TiO2-NPs).

The present work studied the impact of different levels of PVC-microplastics (PVC-MPs), namely 0 (no PVC-MPs), 2, and 4 mg L-1, along with mercury (Hg) levels of 0 (no Hg), 10, and 25 mg kg-1 in the soil, while concurrently applying titanium dioxide-nanoparticles (TiO2-NPs) at 0 (no TiO2-NPs), 50, and 100 µg mL-1 to sorghum (Pennisetum glaucum L.) plants. This study aimed to examine plant growth and biomass, photosynthetic pigments and gas exchange characteristics, oxidative stress indicators, and the response of various antioxidants (enzymatic and non-enzymatic) and their specific gene expression, proline metabolism, the AsA-GSH cycle, and cellular fractionation in the plants. The research outcomes indicated that elevated levels of PVC-MPs and Hg stress in the soil notably reduced plant growth and biomass, photosynthetic pigments, and gas exchange attributes. However, PVC-MPs and Hg stress also induced oxidative stress in the roots and shoots of the plants by increasing malondialdehyde (MDA), hydrogen peroxide (H2O2), and electrolyte leakage (EL) which also induced increased compounds of various enzymatic and non-enzymatic antioxidants and also the gene expression and sugar content. Furthermore, a significant increase in proline metabolism, the AsA-GSH cycle, and the pigmentation of cellular components was observed. Although, the application of TiO2-NPs showed a significant increase in plant growth and biomass, gas exchange characteristics, enzymatic and non-enzymatic compounds, and their gene expression and also decreased oxidative stress. In addition, the application of TiO2-NPs enhanced cellular fractionation and decreased the proline metabolism and AsA-GSH cycle in P. glaucum plants. These results open new insights for sustainable agriculture practices and hold immense promise in addressing the pressing challenges of heavy metal contamination in agricultural soils.

Genome-wide identification of CCO gene family in cucumber (Cucumis sativus) and its comparative analysis with A. thaliana.

Carotenoid cleavage oxygenase (CCO) is an enzyme capable of converting carotenoids into volatile, aromatic compounds and it plays an important role in the production of two significant plant hormones, i.e., abscisic acid (ABA) and strigolactone (SL). The cucumber plant genome has not been mined for genomewide identification of the CCO gene family. In the present study, we conducted a comprehensive genome-wide analysis to identify and thoroughly examine the CCO gene family within the genomic sequence of Cucumis sativus L. A Total of 10 CCO genes were identified and mostly localized in the cytoplasm and chloroplast. The CCO gene is divided into seven subfamilies i.e. 3 NCED, 3 CCD, and 1 CCD-like (CCDL) subfamily according to phylogenetic analysis. Cis-regulatory elements (CREs) analysis revealed the elements associated with growth and development as well as reactions to phytohormonal, biotic, and abiotic stress conditions. CCOs were involved in a variety of physiological and metabolic processes, according to Gene Ontology annotation. Additionally, 10 CCO genes were regulated by 84 miRNA. The CsCCO genes had substantial purifying selection acting upon them, according to the synteny block. In addition, RNAseq analysis indicated that CsCCO genes were expressed in response to phloem transportation and treatment of chitosan oligosaccharides. CsCCD7 and CsNCED2 showed the highest gene expression in response to the exogenous application of chitosan oligosaccharides to improve cold stress in cucumbers. We also found that these genes CsCCD4a and CsCCDL-a showed the highest expression in different plant organs with respect to phloem content. The cucumber CCO gene family was the subject of the first genome-wide report in this study, which may help us better understand cucumber CCO proteins and lay the groundwork for the gene family's future cloning and functional investigations.

Foliar application of iron-lysine to boost growth attributes, photosynthetic pigments and biochemical defense system in canola (Brassica napus L.) under cadmium stress.

In the current industrial scenario, cadmium (Cd) as a metal is of great importance but poses a major threat to the ecosystem. However, the role of micronutrient - amino chelates such as iron - lysine (Fe - lys) in reducing Cr toxicity in crop plants was recently introduced. In the current experiment, the exogenous applications of Fe - lys i.e., 0 and10 mg L - 1, were examined, using an in vivo approach that involved plant growth and biomass, photosynthetic pigments, oxidative stress indicators and antioxidant response, sugar and osmolytes under the soil contaminated with varying levels of Cd i.e., 0, 50 and 100 µM using two different varieties of canola i.e., Sarbaz and Pea - 09. Results revealed that the increasing levels of Cd in the soil decreased plant growth and growth-related attributes and photosynthetic apparatus and also the soluble protein and soluble sugar. In contrast, the addition of different levels of Cd in the soil significantly increased the contents of malondialdehyde (MDA) and hydrogen peroxide (H2O2), which induced oxidative damage in both varieties of canola i.e., Sarbaz and Pea - 09. However, canola plants increased the activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and non-enzymatic compounds such as phenolic, flavonoid, proline, and anthocyanin, which scavenge the over-production of reactive oxygen species (ROS). Cd toxicity can be overcome by the supplementation of Fe - lys, which significantly increased plant growth and biomass, improved photosynthetic machinery and sugar contents, and increased the activities of different antioxidative enzymes, even in the plants grown under different levels of Cd in the soil. Research findings, therefore, suggested that the Fe - lys application can ameliorate Cd toxicity in canola and result in improved plant growth and composition under metal stress.

Fabrication and characterization of novel, cost-effective graphitic carbon nitride/Fe coated textile nanocomposites for effective degradation of dyes and biohazards.

Textile-based photocatalysts are the new materials that can be utilized as an effective sustainable solution for biochemical hazards. Hence, we aimed to develop a sustainable, cost-effective, and facile approach for the fabrication of photocatalytic fabric using graphitic carbon nitride (g-C3N4) and ferric-based multifunctional nanocomposite. Bulk g-C3N4 was prepared from urea by heating it at 500 °C for 2 h. The structure of ball-milled g-C3N4 was engineered by doping with various amounts of iron (III) chloride hexahydrate solution (0.006 mol/L) and sintered at 90 °C for 24 h to prepare g-C3N4-nanosheets/α-Fe2O3 composites. These nanocomposites have potential avenues towards rational designing of g-C3N4 for improved photocatalytic, antibacterial, and antiviral behavior. The prepared nanocomposite was characterized for its surface morphology, chemical composition, crystal structure, catalytic, antibacterial, and antiviral behavior. The fabrication of ferric doped g-C3N4 nanocomposites was characterized by SEM, EDX, FTIR, and XRD analysis. The coated fabric nanocomposite was characterized for methylene blue dye degradation under visible light, antibacterial and antiviral behavior. The developed textile-based photocatalyst has been found with very good recyclability with photocatalytic degradation of dye up to 99.9 % when compared to conventional g-C3N4 powder-based photocatalyst.

Photo-Induced Super-Hydrophilicity of Nano-Calcite @ Polyester Fabric: Enhanced Solar Photocatalytic Activity against Imidacloprid.

The present study is pertinent to photo-induced, hydrophilic, nano-calcite grown onto the mercerized surface of polyester fabric (PF), treated with UV (10-50 min) and visible light (1-5 h) in addition to its photocatalytic application. The wicking method has been employed to select the most hydrophilic sample of fabric upon irradiation. The micrographs obtained by scanning electron microscopy, transmission electron microscopy, and high-resolution transmission electron microscopy indicated the erosions occurring at the surface of nano-calcite after UV light irradiation, maintaining the crystallinity of the photocatalyst. The surface charge has been measured for as-fabricated and irradiated nano-calcite @ PF for the development of high negative zeta potential after UV light irradiation (-24.6 mV). The irradiated nano-calcite @ PF exhibited a significant change in its contact angle, and the wetting property was enhanced to a considerable extent on UV (55.32°) and visible light irradiation (79.00°) in comparison to as-fabricated nano-calcite @ PF (137.54°). The irradiated samples of nano-calcite @ PF delineated the redshift in harvesting of solar spectrum, as revealed by diffuse reflectance spectroscopy comparative spectra. Additionally, the band gap of untreated nano-calcite was found to be 3.5 eV, while UV- and visible light-irradiated PF showed a reduction in band gap up to 2.95 and 3.15 eV upon UV and visible light irradiation. The photocatalytic efficiency of mesoporous nano-calcite was evaluated by photocatalytic degradation of imidacloprid as the probe pollutant. Higher solar photocatalytic degradation of imidacloprid (94.15%) was attained by UV light-irradiated nano-calcite @ PF. The time-resolved photoluminescence study has verified the high photocatalytic activity of UV light-irradiated nano-calcite @ PF for the generation of high concentration of hydroxyl radicals. The highly efficient reusability of a nano-calcite-based solar photocatalytic reactor has been observed for 10 cycles of treatment of imidacloprid bearing wastewater. The enhanced photocatalytic activity of UV light-exposed (20 min), superhydrophilic, nano-calcite @ PF for mineralization of pollutants suggests it to be an efficient solar photocatalyst for environmental applications.

Alkaline and acidic soil constraints on iron accumulation by Rice cultivars in relation to several physio-biochemical parameters.

Agricultural production is severely limited by an iron deficiency. Alkaline soils increase iron deficiency in rice crops, consequently leading to nutrient deficiencies in humans. Adding iron to rice enhances both its elemental composition and the nutritional value it offers humans through the food chain. The purpose of the current pot experiment was to investigate the impact of Fe treatment in alkaline (pH 7.5) and acidic (pH 5.5) soils to introduce iron-rich rice. Iron was applied to the plants in the soil in the form of an aqueous solution of FeSO4 with five different concentrations (100, 200, 300, 400, and 500 mM). The results obtained from the current study demonstrated a significant increase in Fe content in Oryza sativa with the application of iron in both alkaline and acidic pH soils. Specifically, Basmati-515, one of the rice cultivars tested, exhibited a notable 13% increase in iron total accumulation per plant and an 11% increase in root-to-shoot ratio in acidic soil. In contrast to Basmati-198, which demonstrated maximum response in alkaline soil, Basmati-515 exhibited notable increases in all parameters, including a 31% increase in dry weight, 16% increase in total chlorophyll content, an 11% increase in CAT (catalase) activity, 7% increase in APX (ascorbate peroxidase) activity, 26% increase in POD (peroxidase) activity, and a remarkable 92% increase in SOD (superoxide dismutase) in acidic soil. In alkaline soil, Basmati-198 exhibited respective decreases of 40% and 39% in MDA and H2O2 content, whereas Basmati-515 demonstrated a more significant decrease of 50% and 67% in MDA and H2O2 in acidic soil. These results emphasize the potential for targeted soil management strategies to improve iron nutrition and address iron deficiency in agricultural systems. By considering soil conditions, it is possible to enhance iron content and promote its availability in alkaline and acidic soils, ultimately contributing to improved crop nutrition and human health.

Ameliorative Effects of Exogenous Potassium Nitrate on Antioxidant Defense System and Mineral Nutrient Uptake in Radish (Raphanus sativus L.) under Salinity Stress.

Soil salinization has become a major issue around the world in recent years, as it is one of the consequences of climate change as sea levels rise. It is crucial to lessen the severe consequences of soil salinization on plants. A pot experiment was conducted to regulate the physiological and biochemical mechanisms in order to evaluate the ameliorative effects of potassium nitrate (KNO3) on Raphanus sativus L. genotypes under salt stress. The results from the present study illustrated that the salinity stress induced a significant decrease in shoot length, root length, shoot fresh weight, shoot dry weight, root fresh weight, root dry weight, number of leaves per plant, leaf area chlorophyll-a, chlorophyll-b, total chlorophyll, carotenoid, net photosynthesis, stomatal conductance, and transpiration rate by 43, 67, 41, 21, 34, 28, 74, 91, 50, 41, 24, 34, 14, 26, and 67%, respectively, in a 40 day radish while decreased by 34, 61, 49, 19, 31, 27, 70, 81, 41, 16, 31, 11, 21, and 62%, respectively, in Mino radish. Furthermore, MDA, H2O2 initiation, and EL (%) of two varieties (40 day radish and Mino radish) of R. sativus increased significantly (P < 0.05) by 86, 26, and 72%, respectively, in the roots and also increased by 76, 106, and 38% in the leaves in a 40 day radish, compared to the untreated plants. The results also elucidated that the contents of phenolic, flavonoids, ascorbic acid, and anthocyanin in the two varieties (40 day radish and Mino radish) of R. sativus increased with the exogenous application of KNO3 by 41, 43, 24, and 37%, respectively, in the 40 day radish grown under the controlled treatments. Results indicated that implementing KNO3 exogenously in the soil increased the activities of antioxidants like SOD, CAT, POD, and APX by 64, 24, 36, and 84% in the roots and also increased by 21, 12, 23, and 60% in the leaves of 40 day radish while also increased by 42, 13, 18, and 60% in the roots and also increased by 13, 14, 16, and 41% in the leaves in Mino radish, respectively, in comparison to those plants grown without KNO3. We found that KNO3 substantially improved plant growth by lowering the levels of oxidative stress biomarkers, thereby further stimulating the antioxidant potential system, which led to an improved nutritional profile of both R. sativus L. genotypes under normal and stressed conditions. The current study would offer a deep theoretical foundation for clarifying the physiological and biochemical mechanisms by which the KNO3 improves salt tolerance in R. sativus L. genotypes.

Modulation of salt stress through application of citrate capped silver nanoparticles and indole acetic acid in maize.

The present study was conducted to determine the effect of indole acetic acid (IAA) and Citrate Capped Silver Nanoparticles (Cit-AgNPs) on various attributes of maize under induced salinity stress. Seeds of the said variety were collected from Cereal Crop Research Institute (CCRI) Pirsabaq, Nowshera, sterilized and sown in earthen pots filled with 2 kg silt and soil (1:2) in triplicates in the green house of the Botany Department, University of Peshawar. Nanoparticles were analyzed by scanning electron microscopy (SEM), Energy Dispersive X-Ray Spectroscopy (EDX), Thermo-gravimetric analysis (TGA) and Differential thermal analysis (DTA). Results of SEM revealed spherical morphology of Cit-AgNPs while EDX showed various elemental composition. TGA showed dominant weight loss up to 300 °C while the DTA showed major exothermic peaks at 420 °C. High Salinity concentration (80 mM) imposed significant detrimental impacts by reducing the agronomic attributes, photosynthetic pigments, osmolytes and antioxidant enzymes, which was remarkably ameliorated by the foliar application of Cit-AgNPs and IAA. Agronomic attributes including leaf, root and shoot fresh and dry weight was improved by 52-74%, 43-69% and 36-79% in individual as well as combined treatments of IAA and NPs. Photosynthetic pigments were amplified by 35-63%, total osmolytes were augmented by 39-68% and antioxidant enzymes including SOD and POD were boosted by 42-57% and 37-62% respectively, in combined as well as individual application. Conclusively, Cit-AgNPs are considered as salt mitigating entities that enhance the tolerance level of crop plants along with IAA, which may be beneficial for the plants growing in saline stressed environment.

Nitric oxide confers cadmium tolerance in fragrant rice by modulating physio-biochemical processes, yield attributes, and grain quality traits.

Cadmium (Cd) stress causes serious disruptions in plant metabolism, physio-biochemical processes, crop yield, and quality characters. Nitric oxide (NO) improves the quality features and nutritional contents of fruit plants. However, how NO confers Cd toxicity in fragrant rice plants, is sparse. Hence, the present study investigated the effects of 50 µM NO donor sodium nitroprusside (SNP) on physio-biochemical processes, plant growth attributes, grain yield, and quality traits of fragrant rice under Cd stress (100 mg kg-1 soil). The results revealed that Cd stress diminished rice plant growth, impaired photosynthetic apparatus and antioxidant defense system, and deteriorated the grain quality traits. However, foliar application of SNP mitigated Cd stress by improving plant growth and gas exchange attributes. Higher electrolyte leakage (EL) was accompanied with elevated levels of malondialdehyde (MDA) and hydrogen peroxide (H2O2) under Cd stress; however, exogenous application of SNP reduced them. The activities and relative expression levels of enzymatic antioxidants; superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX) and non-enzymatic antioxidants, glutathione (GSH) contents were reduced by Cd stress, while SNP application regulated their activity and transcript abundances. SNP application improved fragrant rice grain yield and 2-acetyl-1-pyrroline content by 57.68 % and 75.54 % respectively, which is concomitant with higher biomass accumulation, photosynthetic efficiency, photosynthetic pigment contents, and an improved antioxidant defense system. Collectively, our results concluded that SNP application regulated the fragrant rice plant physio-biochemical processes, yield traits and grain quality characters under Cd-affected soil.

Potential application of melatonin in reducing boron toxicity in rice seedlings through improved growth, cell wall composition, proline, and defense mechanisms.

Melatonin (MT) has been demonstrated to provide defense against both biotic and abiotic stressors. Boron toxicity (BT) can significantly limit the growth and production of plants. However, few studies have been conducted on whether MT is effective in attenuating B toxicity in different plants. In order to evaluate the efficacy of exogenous MT treatment in reducing the negative impact of BT on rice seedlings, this study examined the influence of MT on growth, antioxidant capacity, cell wall composition, and proline metabolism in rice seedlings under hydroponics. Four treatments were established: MT (50 µM), MT + BT (50 µM MT + 800 µM B), BT (800 µM), and CK (control) in a completely randomized design. The results indicate that BT had a significant detrimental effect on the shoot length, root length, and root and shoot fresh weights of rice seedlings by 11.96%, 27.77%, 25.69%, and 18.67%, respectively as compared to the control treatment. However, exogenous MT application increased these parameters and reduced B accumulation in aboveground parts (14.05%) of the plant. Exogenous MT also increased the endogenous melatonin content and antioxidant enzyme activities (64.45%, 71.61%, 237.64%, and 55.42% increase in superoxide dismutase, ascorbate peroxidase, and peroxidase activities, respectively), while decreasing reactive oxygen species levels and oxidized forms of glutathione and ascorbic acid. Additionally, MT enhanced the biosynthesis of proline by decreasing proline dehydrogenase (ProDH) and increasing the GSH (glutathione) and ASA (ascorbic acid) contents. Exogenous MT also increased cell wall components that can increase B adsorption to the cell wall. Overall, these findings suggest that MT application can be a potential solution for strengthening the stress tolerance of rice seedlings, particularly under conditions of B toxicity. In regions where soil contains high levels of boron, the use of MT could enhance rice crop yields and quality.

Silicic and Ascorbic Acid Induced Modulations in Photosynthetic, Mineral Uptake, and Yield Attributes of Mung Bean (Vigna radiata L. Wilczek) under Ozone Stress.

Most of the world's crop production and plant growth are anticipated to be seriously threatened by the increasing tropospheric ozone (O3) levels. The current study demonstrates how different mung bean genotypes reacted to the elevated level of O3 in the presence of exogenous ascorbic and silicic acid treatments. It is the first report to outline the potential protective effects of ascorbic and silicic acid applications against O3 toxicity in 12 mung bean {Vigna radiata (L.) Wilken} varieties. Under controlled circumstances, the present investigation was conducted in a glass house. There were four different treatments used: control (ambient O3 concentration of 40-45 ppb), elevated O3 (120 ppb), elevated O3 with silicic acid (0.1 mM), and elevated O3 with ascorbic acid (10 mM). Three varieties, viz. NM 20-21, NM 2006, and NM 2016, showcased tolerance to O3 toxicity. Our findings showed that ascorbic and silicic acid applications gradually increased yield characteristics such as seed yield, harvest index, days to maturity, and characteristics related to gas exchange such as transpiration rate, stomatal conductance, net photosynthetic activity, and water-use efficiency. Compared to the control, applying both growth regulators enhanced the mineral uptake across all treatments. Based on the findings of the current study, it is concluded that the subject mung bean genotypes responded to silicic acid treatment more efficiently than ascorbic acid to mitigate the harmful effects of O3 stress.

Genome Editing Technology: A New Frontier for the Treatment and Prevention of Cardiovascular Diseases.

Over the past 2 decades, genome-editing technique has proven to be a robust editing method that revolutionizes the field of biomedicine. At the genetic level, it can be efficiently utilized to generate various disease-resistance models to elucidate the mechanism of human diseases. It also develops an outstanding tool and enables the generation of genetically modified organisms for the treatment and prevention of various diseases. The versatile and novel clustered regularly interspaced short palindromic repeats (CRISPR/Cas9) system mitigates the challenges of various genome editing techniques such as zinc-finger nucleases, and transcription activator-like effector nucleases. For this reason, it has become a ground-breaking technology potentially employed to manipulate the desired gene of interest. Interestingly, this system has been broadly utilized due to its tremendous applications for treating and preventing tumors and various rare disorders; however, its applications for treating cardiovascular diseases (CVDs) remain in infancy. More recently, 2 newly developed genome editing techniques, such as base editing and prime editing, have further broadened the accuracy range to treat CVDs under consideration. Furthermore, recently emerged CRISPR tools have been potentially applied in vivo and in vitro to treat CVDs. To the best of our knowledge, we strongly enlightened the applications of the CRISPR/Cas9 system that opened a new window in the field of cardiovascular research and, in detail, discussed the challenges and limitations of CVDs.

Efficacy of priming wheat (Triticum aestivum) seeds with a benzothiazine derivative to improve drought stress tolerance.

We evaluated the effects of different concentrations (0.05 and 0.15mM) of a benzothiazine (BTh) derivative on wheat (Triticum aestivum L.) in normal (100% field water capacity, FWC) and drought (60% FWC) conditions. Various morphological and physiological characteristics, and the uptake of osmo-protectants and nutrients were measured under the two FWC conditions. Results show that the drought conditions significantly reduced plant growth, affected plant composition, reduced the concentrations of photosynthetic pigments and affected gaseous exchange attributes, stomatal behaviour, and uptake fluxes of essential nutrients, while increasing the contents of different osmo-protectants and enzymatic and non-enzymatic antioxidants to decrease the production of reactive oxygen species (ROS) within the cells/tissues. However, seed priming with BTh reduced water stress conditions by increasing plant growth and biomass, photosynthetic pigments, stomatal behaviour, different gaseous exchange attributes, and uptake fluxes of essential nutrients compared with unprimed plants. In addition, the plant has a strong antioxidant defense system, which further increased its activities under BTh derivative treatments, to scavenge ROS production and maintain cell turgor under water stress conditions. In conclusion, drought stress-induced oxidative stress and altered the growth of T. aestivum , whereas seed priming increased plant growth and antioxidant production by improving the plant tolerance to drought. We suggest that seed priming with a BTh derivative as an effective priming technique in T. aestivum for reducing drought stress tends to benefit a grower in terms of better growth to fulfil the market demand for food cereals.

Effect of intermittent shade on nitrogen dynamics assessed by 15N trace isotopes, enzymatic activity and yield of Brassica napus L.

Modern era of agriculture is concerned with the environmental influence on crop growth and development. Shading is one of the crucial factors affecting crop growth considerably, which has been neglected over the years. Therefore, a two-year field experiment was aimed to investigate the effects of shading at flowering (S1) and pod development (S2) stages on nitrogen (N) dynamics, carbohydrates and yield of rapeseed. Two rapeseed genotypes (Chuannong and Zhongyouza) were selected to evaluate the effects of shading on 15N trace isotopes, enzymatic activities, dry matter, nitrogen and carbohydrate distribution and their relationship with yield. The results demonstrated that both shading treatments disturbed the nitrogen accumulation and transportation at the maturity stage. It was found that shading induced the downregulation of the N mobilizing enzymes (NR, NiR, GS, and GOGAT) in leaves and pods at both developmental stages. Shading at both growth stages resulted in reduced dry matter of both varieties but only S2 exhibited the decline in pod shell and seeds dry weight in both years. Besides this, carbohydrates distribution toward economic organs was declined by S2 treatment and its substantial impact was also experienced in seed weight and seeds number per pod which ultimately decreased the yield in both genotypes. We also revealed that yield is positively correlated with dry matter, nitrogen content and carbohydrates transportation. In contrast to Chuannong, the Zhongyouza genotype performed relatively better under shade stress. Overall, it was noticed that shading at pod developmental stage considerable affected the transportation of N and carbohydrates which led to reduced rapeseed yield as compared to shading at flowering stage. Our study provides basic theoretical support for the management techniques of rapeseed grown under low light regions and revealed the critical growth stage which can be negatively impacted by low light.