Induced responses to the wheat pathogen: Tan Spot-(Pyrenophora tritici-repentis) in wheat (Triticum aestivum) focus on changes in defence associated and sugar metabolism.

INTRODUCTION: Tan Spot (TS) disease of wheat is caused by Pyrenophora tritici-repentis (Ptr), where most of the yield loss is linked to diseased flag leaves. As there are no fully resistant cultivars available, elucidating the responses of wheat to Ptr could inform the derivation of new resistant genotypes. OBJECTIVES: The study aimed to characterise the flag-leaf metabolomes of two spring wheat cultivars (Triticum aestivum L. cv. PF 080719 [PF] and cv. Fundacep Horizonte [FH]) following challenge with Ptr to gain insights into TS disease development. METHODS: PF and FH plants were inoculated with a Ptr strain that produces the necrotrophic toxin ToxA. The metabolic changes in flag leaves following challenge (24, 48, 72, and 96 h post-inoculation [hpi]) with Ptr were investigated using untargeted flow infusion ionisation-high resolution mass spectroscopy (FIE-HRMS). RESULTS: Both cultivars were susceptible to Ptr at the flag-leaf stage. Comparisons of Ptr- and mock-inoculated plants indicated that a major metabolic shift occurred at 24 hpi in FH, and at 48 hpi in PF. Although most altered metabolites were genotype specific, they were linked to common pathways; phenylpropanoid and flavonoid metabolism. Alterations in sugar metabolism as well as in glycolysis and glucogenesis pathways were also observed. Pathway enrichment analysis suggested that Ptr-triggered alterations in chloroplast and photosynthetic machinery in both cultivars, especially in FH at 96 hpi. In a wheat-Ptr interactome in integrative network analysis, "flavone and flavonol biosynthesis" and "starch and sucrose metabolism" were targeted as the key metabolic processes underlying PF-FH-Ptr interactions. CONCLUSION: These observations suggest the potential importance of flavone and flavonol biosynthesis as well as bioenergetic shifts in susceptibility to Ptr. This work highlights the value of metabolomic approaches to provide novel insights into wheat pathosystems.

Identification of Fusarium head blight resistance loci in two Brazilian wheat mapping populations.

Fusarium head blight (FHB) is a disease of wheat (Triticum aestivum L.) that causes major yield losses in South America, as well as many other wheat growing regions around the world. FHB results in low quality, contaminated grain due to the production of mycotoxins such as deoxynivalenol (DON). In Brazil, FHB outbreaks are increasing in frequency and are currently controlled by fungicides which are costly and potentially harmful to the wider environment. To identify the genetic basis of resistance to FHB in Brazilian wheat, two mapping populations (Anahuac 75 × BR 18-Terena and BR 18-Terena × BRS 179) segregating for FHB resistance were phenotyped and quantitative trait loci (QTL) analysis was undertaken to identify genomic regions associated with FHB-related traits. A total of 14 QTL associated with FHB visual symptoms were identified, each of which explained 3.7-17.3% of the phenotypic variance. Two of these QTL were stable across environments. This suggests FHB resistance in Anahuac 75, BR 18-Terena and BRS 179 is controlled by multiple genetic loci that confer relatively minor differences in resistance. A major, novel QTL associated with DON accumulation was also identified on chromosome 4B (17.8% of the phenotypic variance), as well as a major QTL associated with thousand-grain weight on chromosome 6B (16.8% phenotypic variance). These QTL could be useful breeding targets, when pyramided with major sources of resistance such as Fhb1, to improve grain quality and reduce the reliance on fungicides in Brazil and other countries affected by FHB.

Are Demethylation Inhibitor Plus Quinone Outside Inhibitor Fungicide Premixes During Flowering Worthwhile for Fusarium Head Blight Control in Wheat? A Meta-Analysis.

Fusarium head blight (FHB), caused mainly by Fusarium graminearum, is best controlled with demethylation inhibitor (DMI) fungicides during flowering. However, the use of premixes of DMI and quinone outside inhibitor (QoI) fungicides to control FHB has increased in Brazil. Data on FHB severity and wheat yields measured in field experiments conducted in Brazil were gathered from both peer- and nonpeer-reviewed sources published from 2000 to 2018. After selection criteria were applied, 73 field trials from 35 bibliographic sources were identified, among which 50% of the data were obtained from cooperative network trials conducted after 2011. To be included in the analysis, DMI plus QoI premixes or tebuconazole were tested in at least 14 trials and 3 years. Four premixes met the criteria. Estimates of percent control (and respective 95% confidence intervals) by a network model fitted to the log of the treatment means ranged from 44.1% (pyraclostrobin plus metconazole applied once; 32.4 to 53.7) to 64.3% (pyraclostrobin plus metconazole; 58.4 to 69.3); the latter did not differ from tebuconazole (59.9%; 53.6 to 65.3). Yield response was statistically similar for pyraclostrobin plus metconazole (532.1 kg/ha; 441 to 623) and trifloxystrobin plus prothioconazole (494.9 kg/ha; 385 to 551), and both differed statistically from a group composed of tebuconazole (448.2 kg/ha; 342 to 554), trifloxystrobin plus tebuconazole (468.2 kg/ha; 385 to 551), azoxystrobin plus tebuconazole (462.4 kg/ha; 366 to 558), and pyraclostrobin plus metconazole applied once (413.7 kg/ha; 308 to 518). The two categories of FHB index (7% cutoff) and yield (3,000 kg/ha cutoff), both in the nontreated check, did not explain the heterogeneity in the estimates. Considering only the fungicide effects on yield, two sequential sprays of tebuconazole or one spray of pyraclostrobin plus metconazole as management choices are likely more profitable than DMI plus QoI premixes sprayed twice during flowering.

Dissecting the genetic basis of wheat blast resistance in the Brazilian wheat cultivar BR 18-Terena.

BACKGROUND: Wheat blast, caused by Magnaporthe oryzae Triticum (MoT) pathotype, is a global threat to wheat (Triticum aestivum L.) production. Few blast resistance (R) genes have been identified to date, therefore assessing potential sources of resistance in wheat is important. The Brazilian wheat cultivar BR 18-Terena is considered one of the best sources of resistance to blast and has been widely used in Brazilian breeding programmes, however the underlying genetics of this resistance are unknown. RESULTS: BR 18-Terena was used as the common parent in the development of two recombinant inbred line (RIL) F6 populations with the Brazilian cultivars Anahuac 75 and BRS 179. Populations were phenotyped for resistance at the seedling and heading stage using the sequenced MoT isolate BR32, with transgressive segregation being observed. Genetic maps containing 1779 and 1318 markers, were produced for the Anahuac 75 × BR 18-Terena and BR 18-Terena × BRS 179 populations, respectively. Five quantitative trait loci (QTL) associated with seedling resistance, on chromosomes 2B, 4B (2 QTL), 5A and 6A, were identified, as were four QTL associated with heading stage resistance (1A, 2B, 4A and 5A). Seedling and heading stage QTL did not co-locate, despite a significant positive correlation between these traits, indicating that resistance at these developmental stages is likely to be controlled by different genes. BR 18-Terena provided the resistant allele for six QTL, at both developmental stages, with the largest phenotypic effect conferred by a QTL being 24.8% suggesting that BR 18-Terena possesses quantitative resistance. Haplotype analysis of 100 Brazilian wheat cultivars indicates that 11.0% of cultivars already possess a BR 18-Terena-like haplotype for more than one of the identified heading stage QTL. CONCLUSIONS: This study suggests that BR 18-Terena possesses quantitative resistance to wheat blast, with nine QTL associated with resistance at either the seedling or heading stage being detected. Wheat blast resistance is also largely tissue-specific. Identification of durable quantitative resistances which can be combined with race-specific R gene-mediated resistance is critical to effectively control wheat blast. Collectively, this work facilitates marker-assisted selection to develop new varieties for cultivation in regions at risk from this emerging disease.

Genetic Characterization of a Wheat Association Mapping Panel Relevant to Brazilian Breeding Using a High-Density Single Nucleotide Polymorphism Array.

Bread wheat (Triticum aestivum L.) is one of the world's most important crops. Maintaining wheat yield gains across all of its major production areas is a key target toward underpinning global food security. Brazil is a major wheat producer in South America, generating grain yields of around 6.8 million tons per year. Here, we establish and genotype a wheat association mapping resource relevant to contemporary Brazilian wheat breeding programs. The panel of 558 wheat accessions was genotyped using an Illumina iSelect 90,000 single nucleotide polymorphism array. Following quality control, the final data matrix consisted of 470 accessions and 22,475 polymorphic genetic markers (minor allele frequency ≥5%, missing data <5%). Principal component analysis identified distinct differences between materials bred predominantly for the northern Cerrado region, compared to those bred for southern Brazilian agricultural areas. We augmented the genotypic data with 26 functional Kompetitive Allele-Specific PCR (KASP) markers to identify the allelic combinations at genes with previously known effects on agronomically important traits in the panel. This highlighted breeding targets for immediate consideration - notably, increased Fusarium head blight resistance via the Fhb1 locus. To demonstrate the panel's likely future utility, genome-wide association scans for several phenotypic traits were undertaken. Significant (Bonferroni corrected P < 0.05) marker-trait associations were detected for Fusarium kernel damage (a proxy for type 2 Fusarium resistance), identifying previously known quantitative trait loci in the panel. This association mapping panel represents an important resource for Brazilian wheat breeding, allowing future genetic studies to analyze multiple agronomic traits within a single genetically diverse population.

Quantitative Review of the Effects of Triazole and Benzimidazole Fungicides on Fusarium Head Blight and Wheat Yield in Brazil.

Triazole and benzimidazole fungicides have been used for controlling Fusarium head blight (FHB) in wheat for over two decades. In Brazil, it was only during the last five years that uniform fungicide trials for FHB control have been established yearly, thus contributing to a new large body of fungicide efficacy data for this country. A systematic review of both peer- and non-peer-reviewed studies on chemical control conducted since 2000 in Brazil was performed. Fungicides of interest were the triazoles tebuconazole (TEBU1x and TEBU2x) and propiconazole (PROP2x), and the benzimidazole carbendazim (CARB2x). Most fungicides were applied twice, the first at early to mid-flowering and the second 7 to 10 days later. Only TEBU was tested as one or two applications, and thus four treatments were evaluated. For these fungicides, there were 35 trials reporting FHB index and 48 reporting mean yield. Network meta-analytic models were fitted to the data of the log of the means of FHB index for each fungicide and for the nontreated check. The meta-analytic estimates were used to obtain control efficacy ( C¯ ), or percent disease reduction relative to the nontreated check. The absolute mean difference ( D¯ ) in yield (kg/ha) between the fungicide-treated and the nontreated check plots was also estimated. Yield response relative to the nontreated check ( Y¯ ) was also calculated based on the difference in the logs of the means of yield between fungicide-treated and nontreated check. The TEBU1x, TEBU2x, and CARB2x treatments performed similarly with regards to control efficacy (= 59%, 53% and 55%, respectively), and although better than PROP2x (47%), the difference was marginally significant. Yield response ( D¯ ) was highest for TEBU2x, ( D¯ = 558 kg/ha, Y¯ = 19.2%) followed by PROP2x (497 kg/ha, 16.0%), TEBU1x (457 kg/ha, 17.3%), and CARB2x (456 kg/ha, 12.8%). For an average 2016 scenario of fungicide plus application costs (FC = $18 U.S./ha) and average wheat price (WP = $215 U.S./MT), the probability of breaking even on the financial investment in the four treatments ranged from 59 to 63%. For 140 scenarios (four fungicides) created based on the combination of five WP ($133 to 266 U.S./MT) and seven FC ($5 to 35 U.S./ha), the probability of breaking even was >50% for all but two scenarios. The information may serve as a guide for planning future trials and provides a baseline and first step toward optimizing FHB management in Brazil.

Resultados agronômicos e qualitativos da nova cultivar de trigo 'BRS Marcante' / Agronomic and qualitative results of the new wheat cultivar 'BRS Marcante'

A cultivar de trigo 'BRS Marcante' foi desenvolvida pela Embrapa, envolvendo um híbrido F1 do cruzamento entre as linhagens PF 980533 e PF 970227 com a cultivar 'BRS Guamirim', realizado em telado, na Embrapa Trigo, no inverno de 2003. As gerações segregantes foram conduzidas pelo método genealógico e a linhagem resultante, nomeada de PF 080310. A cultivar caracteriza-se pela sua ampla capacidade de adaptação às condições de cultivo do sul do Brasil, pelo bom potencial de rendimento de grãos e qualidade industrial da classe Pão.

The wheat cultivar 'BRS Marcante' was developed by Embrapa, as a result a cross between F1 hybrid between lines PF 980533 and PF 970227 with 'BRS Guamirim' and carried out in a green-house of Embrapa Wheat, on 2003 winter season. The segregate generations were conducted by genealogic method and the genotype resulted was named PF 080310. It has wide adaptation ability to south Brazilian conditions, high grain yield potential and belongs to Bread Class.

Detecção de um begomovírus em amostras foliares de tomateiro com sondas não-radioativas / Detection of a begomovirus in tomato leaf samples using non-radioactive probes

O aumento na ocorrência de begomoviroses (geminiviroses) em tomateiros, Lycopersicon esculentum Mill., em várias regiões brasileiras, vem causando grandes prejuízos para o agronegócio de tomate, devido à ocorrência do inseto vetor, Bemisia argentifolii (Bemisia tabaci biotipo B). A diagnose é realizada em geral por "polymerase chain reaction" (PCR) ou por hibridização com sondas radioativas. A PCR é um método de alta sensibilidade, porém apresenta a desvantagem da possibilidade de obtenção de resultados falso-positivos, devido a contaminações, ou falso-negativos causados por inibidores contaminantes da reação, ou pela extrema especificidade dos iniciadores. A hibridização de ácidos nucléicos com sondas radioativas tem o seu uso limitado devido à necessidade de infra-estrutura especial, treinamento de pessoal, riscos para a saúde do manipulador e demanda constante de radioquímicos. O presente trabalho tem por finalidade demonstrar a viabilidade do uso do método de hibridização com sondas não-radioativas para a detecção de um begomovírus de tomateiro do Brasil. A sensibilidade do teste foi alta, obtendo-se detecção de até 0,1fg do DNA homólogo e em extrato bruto foliar diluído até 100 vezes.

Major outbreaks of tomato begomoviruses (geminiviruses) in several tomato Lycopersicon esculentum Mill growing in many parts of Brazil have been imposing significant losses upon the tomato agribusiness, due to the introduction of the Bemisia argentifolii (Bemisia tabaci biotype B). Polymerase chain reaction (PCR) and hybridization are generally used for diagnosis. The PCR is a detection method with high sensitivity, however it has the disadvantage of producing false-positives, due to contamination, or false-negatives caused by inhibitors or because of the high primer specifity. The use of nucleic acid hybridization with radio-labelled probes is restricted due to the requirement of special infrastructure and handling experience, the risk for the userÆs health and a regular radiochemical element supply. This study is aimed at demonstrating the usefulness of the hybridization method with non-radioactive probes for detection of a tomato begomoviruses in Brazil. The sensitivity of this method was high enabling the detection of 0.1fg of homologous DNA and in crude sap extract diluted up to 100-fold.