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1.
Molecules ; 27(9)2022 Apr 23.
Artículo en Inglés | MEDLINE | ID: mdl-35566076

RESUMEN

Broa is a Portuguese maize bread with characteristic sensory attributes that can only be achieved using traditional maize varieties. This study intends to disclose the volatile compounds that are mainly associated with the baking process of broas, which can be important contributors to their aroma. Twelve broas were prepared from twelve maize flours (eleven traditional maize varieties and one commercial hybrid). Their volatile compounds were analyzed by GC×GC-ToFMS (two-dimensional gas chromatography coupled with time-of-flight mass spectrometry) for an untargeted screening of the chemical compounds mainly formed during baking. It was possible to identify 128 volatiles that belonged to the main chemical families formed during this stage. Among these, only 16 had been previously detected in broas. The most abundant were furans, furanones, and pyranones, but the most relevant for the aroma of broas were ascribed to sulfur-containing compounds, in particular dimethyl trisulfide and methanethiol. Pyrazines might contribute negatively to the aroma of broas since they were present in higher amounts in the commercial broa. This work constitutes the most detailed study of the characterization of broas volatile compounds, particularly those formed during the Maillard reaction. These findings may contribute to the characterization of other maize-based foodstuffs, ultimately improving the production of foods with better sensory features.


Asunto(s)
Compuestos Orgánicos Volátiles , Pan/análisis , Cromatografía de Gases y Espectrometría de Masas/métodos , Humanos , Odorantes/análisis , Compuestos de Azufre/análisis , Compuestos Orgánicos Volátiles/análisis , Zea mays/química
2.
Nursing (Ed. bras., Impr.) ; 24(283): 6689-6702, dez. 2021. tab
Artículo en Inglés, Portugués | LILACS, BDENF - Enfermería | ID: biblio-1371306

RESUMEN

Objetivo: Averiguar o que diz a literatura quanto à satisfação das puérperas em relação à assistência recebida no trabalho de parto e parto nos serviços de saúde. Método: Estudo qualitativo do tipo revisão integrativa, nas Bases de Dados: MEDLINE/BVS, SciELO, BDENF, PubMed, e DOAJ, no período de 15 de janeiro a 20 de fevereiro de 2021. Foi realizada leitura analítica dos estudos identificando os pontos chave para hierarquização e síntese das ideias. Resultados: Foram selecionados 14 estudos. Os dados obtidos com a pesquisa apontam para a inexistência de unanimidade quanto à percepção e satisfação das mulheres em relação à assistência ao trabalho de parto e parto. Conclusão: Portanto, atenção, acolhimento, cuidado e vínculo são elementos fundamentais para uma melhor assistência e, consequentemente, para o alcance da satisfação destas mulheres em relação à assistência recebida(AU)


Objective: To investigate what the literature says about the satisfaction of postpartum women in relation to the assistance received in labor and delivery in health services. Method: Qualitative study of the integrative review type, in Databases: MEDLINE/BVS, SciELO, BDENF, PubMed, and DOAJ, from January 15th to February 20th of 2021. An analytical reading of the studies was carried out, identifying the key points for the hierarchy and synthesis of ideas. Results: 14 studies were selected. The data obtained from the survey point to the lack of unanimity regarding the perception and satisfaction of women in relation to care during labor and delivery. Conclusion: Therefore, attention, reception, care and bonding are fundamental elements for better care and, consequently, for achieving these women's satisfaction in relation to the care received.(AU)


Objetivo: Investigar lo que dice la literatura sobre la satisfacción de las mujeres posparto en relación a la asistencia recibida en el trabajo de parto y parto en los servicios de salud. Método: Estudio cualitativo del tipo revisión integrativa, en Bases de datos: MEDLINE / BVS, SciELO, BDENF, PubMed, y DOAJ, del 15 de enero al 20 de febrero de 2021. Análisis analítico Se realizó la lectura de los estudios, identificando los puntos clave para la jerarquía y síntesis de las ideas. Resultados: Se seleccionaron 14 estudios. Los datos obtenidos de la encuesta apuntan a la falta de unanimidad en cuanto a la percepción y satisfacción de las mujeres en relación al cuidado durante el trabajo de parto y parto. Conclusión: Por lo tanto, la atención, la recepción, el cuidado y la vinculación son elementos fundamentales para un mejor cuidado y, en consecuencia, para lograr la satisfacción de estas mujeres en relación con el cuidado recibido(AU)


Asunto(s)
Satisfacción del Paciente , Periodo Posparto , Parto Normal
3.
Rev Esc Enferm USP ; 55: e03706, 2021.
Artículo en Inglés, Portugués | MEDLINE | ID: mdl-34190878

RESUMEN

OBJECTIVE: To correlate the biometric measurements of the nasal area of premature and term newborns to provide parameters for a nasal protector model. METHOD: A crosssectional descriptive study, carried out in the neonatal joint accommodation, intermediate and intensive care units of a hospital in Maceio, Alagoas, with a total of 300 newborns, divided into two groups: 150 term and 150 premature. Neonatal history data and 1200 digital photographs were used for biometric measurements. RESULTS: The groups were homogeneous regarding gender, weight and length of the newborn. The measurements of nasal width, distance from the wing of the nose to the right and left columella midline, right and left nasal introitus area, length of the right and left nasal dorsum were different when compared in groups according to gestational age and weight ranges - very low weight, low weight and appropriate weight for gestational age (p<0.05). CONCLUSION: The data obtained provide parameters for creating a nasal protector for newborns using prongs, considering anatomical aspects.


Asunto(s)
Nariz , Estudios Transversales , Edad Gestacional , Humanos , Recién Nacido
4.
Rev Bras Ginecol Obstet ; 43(5): 403-413, 2021 May.
Artículo en Inglés | MEDLINE | ID: mdl-34182584

RESUMEN

OBJECTIVE: To investigate in the literature the studies on the benefits of music therapy interventions among pregnant women in the prenatal, delivery and postpartum periods. DATA SOURCES: The search for articles was carried out in the following electronic databases: VHL, LILACS, SciELO, Portal CAPES, PsycINFO, ERIC, PubMed/Medline, and journals specialized in this field: Revista Brasileira de Musicoterapia ("Brazilian Journal of Music Therapy") and Voices. STUDY SELECTION: Descriptors in Portuguese (musicoterapia, gravidez, gestantes, revisão), English (music therapy, pregnancy, pregnant women, review) and Spanish (musicoterapia, embarazo, mujeres embarazadas, revisión) were used. The search was delimited between January 2009 and June 2019. The process of selection and evaluation of the articles was performed through peer review. DATA COLLECTION: The following data were extracted: article title, year of publication, journal, author(s), database, country and date of collection, purpose of the study, sample size, type of care, intervention, instruments used, results, and conclusion. The data were organized in chronological order based on the year of publication of the study. SUMMARY OF THE DATA: In total, 146 articles were identified, and only 23 studies were included in this systematic review. The articles found indicate among their results relaxation, decreased levels of anxiety, psychosocial stress and depression, decreased pain, increase in the maternal bond, improvement in the quality of sleep, control of the fetal heart rate and maternal blood pressure, and decreased intake of drugs in the postoperative period. CONCLUSION: Music therapy during the prenatal, delivery and postpartum periods can provide benefits to pregnant women and newborns, thus justifying its importance in this field.


OBJETIVO: Investigar na literatura os estudos sobre os benefícios das intervenções musicoterapêuticas em gestantes no pré-natal, parto e pós-parto. FONTES DOS DADOS: A busca dos artigos foi realizada nas seguintes bases de dados eletrônicas: BVS, LILACS, SciELO, Portal CAPES, PsycINFO, ERIC, PubMed/Medline e revistas especializadas da área: Revista Brasileira de Musicoterapia e Voices. SELEçãO DOS ESTUDOS: Utilizaram-se descritores em português (musicoterapia, gravidez, gestantes, revisão), em inglês (music therapy, pregnancy, pregnant women, review) e em espanhol (musicoterapia, embarazo, mujeres embarazadas, revisión). A busca foi delimitada de janeiro de 2009 até junho de 2019. Os processos de seleção e avaliação dos artigos foram realizados por revisão por pares. COLETA DE DADOS: Os seguintes dados foram extraídos: título do artigo, ano da publicação, revista, autor(es), base de dados, país e data da coleta, objetivo do estudo, tamanho da amostra, tipo de atendimento, intervenção, instrumentos utilizados, resultados, e conclusão. Os dados foram organizados em ordem cronológica a partir do ano de publicação do estudo. SíNTESE DOS DADOS: Foram identificados 146 artigos e incluídos apenas 23 estudos na revisão sistemática. Os artigos encontrados indicam em seus resultados relaxamento, diminuição dos níveis de ansiedade, de estresse psicossocial e de depressão, diminuição da dor, aumento do vínculo materno, melhora da qualidade do sono, controle da frequência cardíaca fetal e da pressão arterial materna, e diminuição da ingestão de fármacos no pós-operatório. CONCLUSõES: A musicoterapia durante o pré-natal, parto e pós-parto pode trazer benefícios para a gestante e para o neonato, o que justifica sua importância nessa área.


Asunto(s)
Musicoterapia , Música/psicología , Mujeres Embarazadas/psicología , Femenino , Humanos , Parto , Periodo Posparto , Embarazo , Atención Prenatal , Calidad de Vida , Relajación , Salud de la Mujer
5.
Rev. bras. ginecol. obstet ; 43(5): 403-413, May 2021. tab, graf
Artículo en Inglés | LILACS | ID: biblio-1288560

RESUMEN

Abstract Objective To investigate in the literature the studies on the benefits ofmusic therapy interventions among pregnant women in the prenatal, delivery and postpartum periods. Data Sources The search for articles was carried out in the following electronic databases: VHL, LILACS, SciELO, Portal CAPES, PsycINFO, ERIC, PubMed/Medline, and journals specialized in this field: Revista Brasileira de Musicoterapia ("Brazilian Journal of Music Therapy") and Voices. Study Selection Descriptors in Portuguese (musicoterapia, gravidez, gestantes, revisão), English (music therapy, pregnancy, pregnant women, review) and Spanish (musicoterapia, embarazo, mujeres embarazadas, revisión) were used. The search was delimited between January 2009 and June 2019. The process of selection and evaluation of the articles was performed through peer review. Data Collectio n The following data were extracted: article title, year of publication, journal, author(s), database, country and date of collection, purpose of the study, sample size, type of care, intervention, instruments used, results, and conclusion. The data were organized in chronological order based on the year of publication of thestudy. Summary of the Data In total, 146 articles were identified, and only 23 studies were included in this systematic review. The articles found indicate among their results relaxation, decreased levels of anxiety, psychosocial stress and depression, decreased pain, increase in the maternal bond, improvement in the quality of sleep, control of the fetal heart rate and maternal blood pressure, and decreased intake of drugs in the postoperative period. Conclusion Music therapy during the prenatal, delivery and postpartum periods can provide benefits to pregnant women and newborns, thus justifying its importance in this field.


Resumo Objetivo Investigar na literatura os estudos sobre os benefícios das intervenções musicoterapêuticas em gestantes no pré-natal, parto e pós-parto. Fontes dos dados A busca dos artigos foi realizada nas seguintes bases de dados eletrônicas: BVS, LILACS, SciELO, Portal CAPES, PsycINFO, ERIC, PubMed/Medline e revistas especializadas da área: Revista Brasileira de Musicoterapia e Voices. Seleção dos estudos Utilizaram-se descritores em português (musicoterapia, gravidez, gestantes, revisão), em inglês (music therapy, pregnancy, pregnant women, review) e em espanhol (musicoterapia, embarazo, mujeres embarazadas, revisión). A busca foi delimitada de janeiro de 2009 até junho de 2019. Os processos de seleção e avaliação dos artigos foram realizados por revisão por pares. Coleta de dados Os seguintes dados foram extraídos: título do artigo, ano da publicação, revista, autor(es), base de dados, país e data da coleta, objetivo do estudo, tamanho da amostra, tipo de atendimento, intervenção, instrumentos utilizados, resultados, e conclusão. Os dados foram organizados emordem cronológica a partir do ano de publicação do estudo. Síntese dos dados Foram identificados 146 artigos e incluídos apenas 23 estudos na revisão sistemática. Os artigos encontrados indicam em seus resultados relaxamento, diminuição dos níveis de ansiedade, de estresse psicossocial e de depressão, diminuição da dor, aumento do vínculo materno, melhora da qualidade do sono, controle da frequência cardíaca fetal e da pressão arterial materna, e diminuição da ingestão de fármacos no pós-operatório. Conclusões A musicoterapia durante o pré-natal, parto e pós-parto pode trazer benefícios para a gestante e para o neonato, o que justifica sua importância nessa área.


Asunto(s)
Humanos , Femenino , Embarazo , Mujeres Embarazadas/psicología , Música/psicología , Musicoterapia , Atención Prenatal , Calidad de Vida , Relajación , Salud de la Mujer , Parto , Periodo Posparto
6.
Rev. Esc. Enferm. USP ; 55: e03706, 2021. tab
Artículo en Inglés | LILACS, BDENF - Enfermería | ID: biblio-1279631

RESUMEN

ABSTRACT Objective: To correlate the biometric measurements of the nasal area of premature and term newborns to provide parameters for a nasal protector model. Method: A crosssectional descriptive study, carried out in the neonatal joint accommodation, intermediate and intensive care units of a hospital in Maceio, Alagoas, with a total of 300 newborns, divided into two groups: 150 term and 150 premature. Neonatal history data and 1200 digital photographs were used for biometric measurements. Results: The groups were homogeneous regarding gender, weight and length of the newborn. The measurements of nasal width, distance from the wing of the nose to the right and left columella midline, right and left nasal introitus area, length of the right and left nasal dorsum were different when compared in groups according to gestational age and weight ranges - very low weight, low weight and appropriate weight for gestational age (p<0.05). Conclusion: The data obtained provide parameters for creating a nasal protector for newborns using prongs, considering anatomical aspects.


RESUMEN Objetivo: Correlacionar las medidas biométricas de la región nasal de neonatos prematuros y a término y ofrecer parámetros para un modelo de protector nasal. Método: Se realizó un estudio descriptivo de corte transversal en unidades de internación conjunta de cuidados intermediarios e intensivos neonatales de un hospital de Maceió, Alagoas, con 300 recién nacidos, divididos en dos grupos: 150 a término y 150 prematuros. Para las mediciones biométricas se utilizaron datos de la historia neonatal y 1.200 fotografías digitales. Resultados: Los grupos eran homogéneos en cuanto a sexo, peso y longitud del recién nacido. Las medidas de la anchura nasal, la distancia del ala de la nariz a la línea media del subtabique, lado derecho e izquierdo, la longitud y la anchura del subtabique, el área de las fosas nasales derecha e izquierda, la longitud del tabique nasal lado derecho e izquierdo eran diferentes cuando se compararon en los grupos según la edad gestacional, y en los rangos de peso: peso muy bajo, peso bajo y peso adecuado para la edad gestacional (p<0,05). Conclusión: Los datos obtenidos brindan parámetros para la creación de un protector nasal para recién nacidos que utilizan prongs, considerando los aspectos anatómicos.


RESUMO Objetivo: Correlacionar as medidas biométricas da região nasal de recém-nascidos prematuros e a termo e fornecer parâmetros para um modelo de protetor nasal. Método: Estudo descritivo de corte transversal, realizado nas unidades de alojamento conjunto, cuidados intermediários e intensivos neonatais de um hospital de Maceió, Alagoas, com 300 recém-nascidos, divididos em dois grupos: 150 a termo e 150 prematuros. Utilizaram-se dados da história neonatal e 1200 fotografias digitais para as medidas biométricas. Resultados: Os grupos foram homogêneos quanto ao sexo, peso e comprimento do recém-nascido. As medidas de largura nasal, distância da asa do nariz à linha média columelar direita e esquerda, comprimento e largura da columela, área do introito nasal direita e esquerda, comprimento do dorso nasal direito e esquerdo apresentaram-se diferentes quando comparadas em grupos de acordo com a idade gestacional, e quanto às faixas de peso - muito baixo peso, baixo peso e peso adequado a idade gestacional (p<0,05). Conclusão: Os dados obtidos fornecem parâmetros para criação de protetor nasal para recém-nascidos em uso de pronga, considerando aspectos anatômicos.


Asunto(s)
Enfermería Neonatal , Tecnología , Recién Nacido , Nariz , Factores Protectores
7.
Hum Mutat ; 41(11): 1999-2011, 2020 11.
Artículo en Inglés | MEDLINE | ID: mdl-32906212

RESUMEN

Clinical and genetic features of five unrelated patients with de novo pathogenic variants in the synaptic vesicle-associated membrane protein 2 (VAMP2) reveal common features of global developmental delay, autistic tendencies, behavioral disturbances, and a higher propensity to develop epilepsy. For one patient, a cognitively impaired adolescent with a de novo stop-gain VAMP2 mutation, we tested a potential treatment strategy, enhancing neurotransmission by prolonging action potentials with the aminopyridine family of potassium channel blockers, 4-aminopyridine and 3,4-diaminopyridine, in vitro and in vivo. Synaptic vesicle recycling and neurotransmission were assayed in neurons expressing three VAMP2 variants by live-cell imaging and electrophysiology. In cellular models, two variants decrease both the rate of exocytosis and the number of synaptic vesicles released from the recycling pool, compared with wild-type. Aminopyridine treatment increases the rate and extent of exocytosis and total synaptic charge transfer and desynchronizes GABA release. The clinical response of the patient to 2 years of off-label aminopyridine treatment includes improved emotional and behavioral regulation by parental report, and objective improvement in standardized cognitive measures. Aminopyridine treatment may extend to patients with pathogenic variants in VAMP2 and other genes influencing presynaptic function or GABAergic tone, and tested in vitro before treatment.


Asunto(s)
4-Aminopiridina/farmacología , Mutación/genética , Proteína 2 de Membrana Asociada a Vesículas/genética , Adulto , Electrofisiología , Exocitosis/efectos de los fármacos , Femenino , Humanos , Masculino , Transmisión Sináptica/efectos de los fármacos , Vesículas Sinápticas/efectos de los fármacos , Vesículas Sinápticas/metabolismo
8.
Rev. chil. nutr ; 47(4): 658-668, ago. 2020. tab, graf
Artículo en Inglés | LILACS | ID: biblio-1138601

RESUMEN

ABSTRACT Regular lentil consumption may improve health and prevent certain chronic diseases. Lentils have high antioxidant activity and contain proteins, essential amino acids, fiber, minerals, and bioactive compounds. However, few studies have investigated the physicochemical characteristics of lentils when subjected to various soaking and cooking methods. We aimed to evaluate the effects of different hydration and cooking practices on the hydration coefficient, cooking time, primary metabolism compounds (lipids, proteins, fibers, carbohydrates), energy value, soluble protein, color and texture characteristics, amino acid and mineral profiles, bioactive compounds, antioxidant activity, and antinutritional factors (phytates and tannins) in lentils. Hydration water was preheated to initial temperatures of 25°C or 90°C. Lentils pre-hydrated with water at 90°C needed less cooking time, obtained greater softness, and had less decreases in amino acids, minerals, bioactive compounds, and antioxidant activity.


RESUMEN El consumo regular de lentejas puede mejorar la salud y prevenir ciertas enfermedades crónicas. Las lentejas tienen una alta actividad antioxidante y contienen proteínas, aminoácidos esenciales, fibra, minerales y compuestos bioactivos. Sin embargo, pocos estudios han investigado las características fisicoquímicas de las lentejas sometidas a varios métodos de remojo y cocción. El objetivo de este estudio fue evaluar los efectos de diferentes procesos de cocción e hidratación sobre el coeficiente de hidratación, el tiempo de cocción, los metabolitos primarios (lípidos, proteínas, fibras, carbohidratos), el valor energético, la proteína soluble, color y textura, los perfiles de aminoácidos y minerales, los compuestos bioactivos, la actividad antioxidante y los factores antinutricionales (fitatos y taninos) en la lenteja. El agua de hidratación se precalentó a temperaturas iniciales de 25°C o 90°C. Las lentejas prehidratadas con agua a 90°C necesitaban menos tiempo de cocción y obtuvieron una mayor suavidad, disminuyendo también la pérdida de aminoácidos, minerales, compuestos bioactivos y actividad antioxidante.


Asunto(s)
Salud , Enfermedad Crónica , Lens (Planta) , Aminoácidos Esenciales , Proteínas , Metabolismo , Minerales , Antioxidantes
9.
Int J Biochem Cell Biol ; 108: 40-50, 2019 03.
Artículo en Inglés | MEDLINE | ID: mdl-30648622

RESUMEN

Staphylococcal food poisoning is a disease that originates significant health and economic losses and is caused by Staphylococcus aureus strains able to produce enterotoxins. The aim of this work is to go further on the study of the volatile exometabolome of S. aureus using an advanced gas chromatographic technique. Enterotoxic and non-enterotoxic strains were assessed. The volatile exometabolome profile comprised 240 volatiles belonging to ten chemical families. This volatiles were mainly by-products of branched-chain amino acids and methionine degradation, pyruvate metabolism, diacetyl pathway, oxidative stress and carotenoid cleavage. Metabolites released by the first two pathways were produced in higher contents by the enterotoxic strains. This study add further insights to S. aureus volatile exometabolome, and also shows that by applying it, it is possible to distinguish strains of S. aureus by the number of produced enterotoxins, which is especially important from the food safety point of view.


Asunto(s)
Metabolómica , Staphylococcus aureus/metabolismo , Especificidad de la Especie , Staphylococcus aureus/aislamiento & purificación , Volatilización
10.
Front Cell Neurosci ; 11: 324, 2017.
Artículo en Inglés | MEDLINE | ID: mdl-29123471

RESUMEN

Release of the major excitatory neurotransmitter glutamate by synaptic vesicle exocytosis depends on glutamate loading into synaptic vesicles by vesicular glutamate transporters (VGLUTs). The two principal isoforms, VGLUT1 and 2, exhibit a complementary pattern of expression in adult brain that broadly distinguishes cortical (VGLUT1) and subcortical (VGLUT2) systems, and correlates with distinct physiological properties in synapses expressing these isoforms. Differential trafficking of VGLUT1 and 2 has been suggested to underlie their functional diversity. Increasing evidence suggests individual synaptic vesicle proteins use specific sorting signals to engage specialized biochemical mechanisms to regulate their recycling. We observed that VGLUT2 recycles differently in response to high frequency stimulation than VGLUT1. Here we further explore the trafficking of VGLUT2 using a pHluorin-based reporter, VGLUT2-pH. VGLUT2-pH exhibits slower rates of both exocytosis and endocytosis than VGLUT1-pH. VGLUT2-pH recycling is slower than VGLUT1-pH in both hippocampal neurons, which endogenously express mostly VGLUT1, and thalamic neurons, which endogenously express mostly VGLUT2, indicating that protein identity, not synaptic vesicle membrane or neuronal cell type, controls sorting. We characterize sorting signals in the C-terminal dileucine-like motif, which plays a crucial role in VGLUT2 trafficking. Disruption of this motif abolishes synaptic targeting of VGLUT2 and essentially eliminates endocytosis of the transporter. Mutational and biochemical analysis demonstrates that clathrin adaptor proteins (APs) interact with VGLUT2 at the dileucine-like motif. VGLUT2 interacts with AP-2, a well-studied adaptor protein for clathrin mediated endocytosis. In addition, VGLUT2 also interacts with the alternate adaptors, AP-1 and AP-3. VGLUT2 relies on distinct recycling mechanisms from VGLUT1. Abrogation of these differences by pharmacological and molecular inhibition reveals that these mechanisms are dependent on the adaptor proteins AP-1 and AP-3. Further, shRNA-mediated knockdown reveals differential roles for AP-1 and AP-3 in VGLUT2 recycling.

11.
Environ Pollut ; 231(Pt 1): 802-811, 2017 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-28865386

RESUMEN

The volatile metabolome of Rhizobium sp. strain E20-8 exposed to three concentrations of cadmium (2.5, 5.0 and 7.5 µM) was screened using comprehensive two-dimensional gas chromatography coupled to time of flight mass spectrometry (GC × GC-ToFMS), combined with headspace solid phase microextraction (HS-SPME). Cd exposure induced a global increase in the concentration of volatile organic compounds (VOCs) both intra and extracellularly. Peak areas of several linear alkanes, ketones, aldehydes, alcohols, terpenic and volatile sulfur compounds, and one ester (ethyl acetate), were especially increased when compared with the control condition (no Cd). These compounds might originate from the metabolization of toxic membrane peroxidation products, the proteolysis of oxidized proteins or the alteration of metabolic pathways, resulting from the oxidative stress imposed by Cd. Several VOCs are related to oxidative damage, but the production of VOCs involved in antioxidant response (menthol, α-pinene, dimethyl sulfide, disulfide and trisulfide, 1-butanol and 2-butanone) and in cell aggregation (2,3-butanedione, 3-methyl-1-butanol and 2-butanone) is also observed. These results bring new information that highlights the role of VOCs on bacteria response to Cd stress, identify a novel set of biomarkers related with metal stress and provide information to be applied in biotechnological and remediation contexts.


Asunto(s)
Cadmio/toxicidad , Sustancias Peligrosas/toxicidad , Rhizobium/fisiología , Pruebas de Toxicidad , Acetatos , Alcoholes/análisis , Aldehídos/análisis , Alcanos/análisis , Monoterpenos Bicíclicos , Butanonas , Ésteres/análisis , Cromatografía de Gases y Espectrometría de Masas/métodos , Cetonas , Monoterpenos , Pentanoles , Microextracción en Fase Sólida/métodos , Compuestos Orgánicos Volátiles/análisis
12.
Curr Microbiol ; 74(5): 575-583, 2017 May.
Artículo en Inglés | MEDLINE | ID: mdl-28260118

RESUMEN

The aim of this study is to evaluate the effects of the inoculation of the saltmarsh plant (Halimione portulacoides) with Pseudomonas sp. SPN31 nah+ combined with exposure to 2-methylnaphthalene (2-MtN) on the plant rhizosphere and endosphere bacterial communities as well as on plant health. To achieve this goal, microcosm experiments were set up. Denaturing gradient gel electrophoresis (DGGE) profiles and statistical analysis showed that rhizosphere and endosphere bacterial communities had distinct responses to plant inoculation and/or exposure to 2-MtN. PCR-sequencing analysis of nah genes encoding for 2-MtN degrading enzymes suggested the presence of Pseudomonas sp. SPN31 nah+ in the endosphere of H. portulacoides with 2-MtN contamination. Moreover, a significant effect in the photosynthetic performance of inoculated plants was detected. To conclude, despite the potential beneficial effect of plant inoculation with Pseudomonas sp. SPN31 nah+ endophytic bacteria may have on plant health, no significant effect on the removal of MtN was detected for the level of contamination used in the study.


Asunto(s)
Bacterias , Chenopodiaceae/efectos de los fármacos , Chenopodiaceae/microbiología , Naftalenos/efectos adversos , Pseudomonas/fisiología , Rizosfera , Contaminantes del Suelo/efectos adversos , Bacterias/clasificación , Bacterias/genética , Biodegradación Ambiental , Chenopodiaceae/fisiología , Regulación Bacteriana de la Expresión Génica , Regulación Enzimológica de la Expresión Génica , Genes Bacterianos , Naftalenos/metabolismo , Fotosíntesis , Filogenia , Raíces de Plantas/microbiología , ARN Ribosómico 16S/genética , Microbiología del Suelo , Contaminantes del Suelo/metabolismo , Compuestos Orgánicos Volátiles
13.
Motriz (Online) ; 23(2): e101628, 2017. tab, ilus
Artículo en Inglés | LILACS | ID: biblio-841844

RESUMEN

Abstract Aims: Cardiovascular disease risk factors occur more frequently in children with obesity. Project PANK is a multidisciplinary school-based intervention lasting 6 months to improve BMI z-score, waist circumference (WC), waist-to-height ratio (WHtR), blood pressure (BP), nutrition, physical activity (PA), sedentary behaviour (SB), cardiorespiratory fitness (CRF), glucose, cholesterol, and triglycerides (TG). Methods/DesignA total of 77 children (7-10 years) were recruited from an urban school. The protocol includes PA and SB individual meetings for children/parents; increasing school exercise; PA and SB lessons for children; A goal in the number of steps/day to accomplish in and after school. In nutrition, the protocol includes three individual meetings for children/parents and six lessons for children. ResultsPositive associations were found between the BMI Z-score, WC, and WHtR with TG; the BMI Z-score and WHtR with glucose; the light PA time and HDL-C; the vigorous and moderate-to-vigorous PA with CRF; the caloric intake and lipids with LDL-C, BMI z-score, WC, and WHtR. A negative association was found between CRF and TG. ConclusionBaseline results stress the importance of multidisciplinary school-based interventions. We hypothesized that PANK will improve blood variables, anthropometric measures, and BP, by changing food intake, enhancing PA and CRF, and decreasing SB.(AU)


Asunto(s)
Humanos , Masculino , Femenino , Niño , Enfermedades Cardiovasculares/etiología , Ejercicio Físico , Síndrome Metabólico , Obesidad/prevención & control , Factores de Riesgo , Conducta Sedentaria
14.
Pesqui. vet. bras ; 36(12): 1194-1202, Dec. 2016. tab, graf, ilus
Artículo en Inglés | LILACS, VETINDEX | ID: biblio-842033

RESUMEN

The maintenance of metabolic activities during the in vitro culture of somatic cells of wild animals, especially collared peccary (Pecari tajacu), is an interesting step in conservation of these cells for the use in nuclear transfer. In this context, it is necessary to optimize the culture conditions of somatic cells by the establishment of appropriate supplementation to the media. Therefore, this study aimed to analyze the composition of the culture means of somatic cell derived from ear tissue of collared peccaries, evaluating concentrations of fetal bovine serum (FBS; 10% vs. 20%) and epidermal growth factor (EGF; 5ng/mL vs. 10ng/mL). Tissues were submitted to primary culture and subcultures for 40 days and cells were analyzed for morphology, adhesion, subconfluence, and proliferative activity to develop the growth curve and to determine the population doubling time (PDT), viability, and functional/metabolic activity. No difference was observed between the concentrations of FBS for several parameters, except for viability [FBS10: 85.6% vs. FBS20: 98.2%], PDT [FBS10: 155.4h vs. 77.2h], and functional/metabolic assay [FBS10: 0.57-0.55 vs. FBS20: 0.82-0.99 (D5-D7)]. For the EGF in culture, no difference was observed in the evaluated parameters. In all experiments, the growth curves were typical S-shape and the cells passed through a lag, logarithmic, and plateau phase. In conclusion, 20% FBS is suitable for the recovery of somatic cells; nevertheless, EGF does not improve the quality of growing these cells. To our knowledge, this is the first study culturing somatic cells of collared peccaries.(AU)


A manutenção das atividades metabólicas durante o cultivo in vitro de células somáticas de animais silvestres, especialmente cateto (Pecari tajacu), é uma etapa interessante na conservação dessas células para o uso na transferência nuclear. Nesse contexto, é necessário aperfeiçoar as condições de cultivo de células somáticas pelo estabelecimento de suplementações apropriadas aos meios. Portanto, este estudo objetivou analisar a composição dos meios de cultivo de células somáticas derivadas de tecido auricular de catetos, avaliando concentrações de soro fetal bovino (SFB; 10% vs. 20%) e fator de crescimento epidermal (EGF; 5 ng/mL vs. 10 ng/mL). Para tanto, tecidos foram submetidos ao cultivo primário e subcultivos por 40 dias e células foram analisadas por morfologia, adesão, subconfluência, e atividade proliferativa pelo desenvolvimento da curva de crescimento e determinação do time de duplicação da população (PDT), viabilidade, e atividade funcional/metabólica. Nenhuma diferença foi observada entre as concentrações de SFB para os vários parâmetros, exceto para viabilidade [SFB10: 85,6% vs. SFB20: 98,2%], PDT [SFB10: 155,4 h vs. 77,2 h], e atividade funcional/metabólica [SFB10: 0,57-0,55 vs. SFB20: 0,82-0,99 (D5-D7)]. Para o EGF em cultivo, nenhuma diferença foi observada nos parâmetros avaliados. Em todos os experimentos, as curvas de crescimento foram típicas de forma S e as células passaram por uma fase lag, logarítmica e platô. Em conclusão, 20% de SFB é adequado para a recuperação de células somáticas; contudo, EGF não melhora a qualidade de crescimento dessas células. Ao nosso conhecimento, este é o primeiro estudo cultivando células somáticas de catetos.(AU)


Asunto(s)
Animales , Artiodáctilos , Células Cultivadas , Oído , Ingeniería de Tejidos/veterinaria , Técnicas In Vitro/veterinaria , Técnicas de Cultivo de Tejidos/veterinaria
15.
Rev. cuba. inf. cienc. salud ; 26(4)oct.-dic. 2015. ilus
Artículo en Español | CUMED | ID: cum-62971

RESUMEN

Con el objetivo de identificar los elementos fuentes del capital social en las organizaciones de información y su relación con el proceso de gestión del conocimiento, se realizó un estudio de tipo descriptivo. Se utilizó el análisis documental clásico y el análisis de contenido. Se muestran los principales autores del tema y otros que lo han tratado desde diversas perspectivas de análisis. Se abordan aspectos teóricos referentes al tema del capital social en las organizaciones y específicamente en las organizaciones de información. Se analizan los criterios sobre sus principales exponentes, dimensiones, niveles y tipos. Se examinan los elementos fuentes del capital social y su identificación en las organizaciones de información. Se expone la estrecha vinculación entre el capital social y el proceso de gestión del conocimiento. Se logró identificar los elementos fuentes de dicho capital en las organizaciones de información. Se constató la relevancia de los elementos del capital social para el desarrollo de la gestión del conocimiento en las organizaciones. El capital social es un activo intangible de esencial identificación en las organizaciones de información, pues su esencia se basa en las relaciones sociales capaces de desarrollar estas entidades en sus tres ambientes de acción: individual, organizacional y externo(AU)


A descriptive study was conducted to identify the source elements of social capital in information organizations and their relationship to the process of knowledge management. The study was based on classic document analysis and content analysis. Reference is made to the main authors dealing with the subject as well as others who have approached it from various perspectives. A discussion is provided of theoretical aspects of the topic of social capital in organizations, particularly in information organizations. An analysis is presented of criteria advanced by the main exponents of social capital, its dimensions, levels and types. Examination is performed of the source elements of social capital and their identification in information organizations. Evidence is provided of the close link between social capital and the process of knowledge management. Identification is performed of the source elements of social capital in information organizations. It was found that social capital elements are relevant to the development of knowledge management in organizations. Social capital is an intangible asset of indispensable identification in information organizations, since its essence is based on social relationships capable of developing those entities on their three dimensions: individual, organizational and external(AU)


Asunto(s)
Gestión del Conocimiento , Centros de Información , Recursos Humanos , Epidemiología Descriptiva
16.
Rev. cub. inf. cienc. salud ; 26(4): 0-0, oct.-dic. 2015. ilus
Artículo en Español | LILACS | ID: lil-769423

RESUMEN

Con el objetivo de identificar los elementos fuentes del capital social en las organizaciones de información y su relación con el proceso de gestión del conocimiento, se realizó un estudio de tipo descriptivo. Se utilizó el análisis documental clásico y el análisis de contenido. Se muestran los principales autores del tema y otros que lo han tratado desde diversas perspectivas de análisis. Se abordan aspectos teóricos referentes al tema del capital social en las organizaciones y específicamente en las organizaciones de información. Se analizan los criterios sobre sus principales exponentes, dimensiones, niveles y tipos. Se examinan los elementos fuentes del capital social y su identificación en las organizaciones de información. Se expone la estrecha vinculación entre el capital social y el proceso de gestión del conocimiento. Se logró identificar los elementos fuentes de dicho capital en las organizaciones de información. Se constató la relevancia de los elementos del capital social para el desarrollo de la gestión del conocimiento en las organizaciones. El capital social es un activo intangible de esencial identificación en las organizaciones de información, pues su esencia se basa en las relaciones sociales capaces de desarrollar estas entidades en sus tres ambientes de acción: individual, organizacional y externo...


A descriptive study was conducted to identify the source elements of social capital in information organizations and their relationship to the process of knowledge management. The study was based on classic document analysis and content analysis. Reference is made to the main authors dealing with the subject as well as others who have approached it from various perspectives. A discussion is provided of theoretical aspects of the topic of social capital in organizations, particularly in information organizations. An analysis is presented of criteria advanced by the main exponents of social capital, its dimensions, levels and types. Examination is performed of the source elements of social capital and their identification in information organizations. Evidence is provided of the close link between social capital and the process of knowledge management. Identification is performed of the source elements of social capital in information organizations. It was found that social capital elements are relevant to the development of knowledge management in organizations. Social capital is an intangible asset of indispensable identification in information organizations, since its essence is based on social relationships capable of developing those entities on their three dimensions: individual, organizational and external...


Asunto(s)
Humanos , Recursos Humanos , Centros de Información , Gestión del Conocimiento , Epidemiología Descriptiva
18.
Plant Foods Hum Nutr ; 70(4): 463-9, 2015 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-26249219

RESUMEN

The characteristics of modern life has led consumers to seek convenience and speed in food preparation, but storage, which is often prolonged, can result in grain hardening, leading to higher energy consumption during preparation, grain with increased hardness is often discarded. Due to the increasing global demand for grain, the use of alternative techniques aimed at reducing grain waste is necessary. Therefore, we studied a method that meets consumer demand and results in better use of harvested grain. The beans studied were cowpea beans (Vigna unguiculata) of the BRS Guariba cultivar after 1 and 12 months of storage. Sodium chloride (NaCl), potassium chloride (KCl) and sodium bicarbonate (NaHCO3) were added to soaking water at different concentrations as a way to reduce cooking time. These beans were placed in soaking water for 12 h at 3 concentrations (0, 1.0 and 2.5%). The cowpeas soaked in water containing NaHCO3 presented the highest hydration coefficient, which increased with the salt concentration; these beans also had decreased hardness, chewiness and cooking time.


Asunto(s)
Fabaceae , Manipulación de Alimentos/métodos , Sales (Química) , Agua , Fenómenos Químicos , Dureza , Calor , Cloruro de Potasio , Bicarbonato de Sodio , Cloruro de Sodio , Factores de Tiempo
19.
Rev. cuba. inf. cienc. salud ; 25(4)oct.-dic. 2014.
Artículo en Español | CUMED | ID: cum-59047

RESUMEN

Objetivo: proponer pautas para identificar el capital cultural en las entidades de información. Métodos: se realizó un estudio de tipo descriptivo. Se utilizaron el análisis documental clásico y las entrevistas a través de las variables clima social, lazos de confianza, canales de comunicación, compromiso y sentido de pertenencia, condiciones de trabajo, competencias laborales, cultura organizacional, valores culturales, satisfacción, experiencia y liderazgo. Se mostraron las definiciones, perspectivas y elementos del capital cultural y se demostró su importancia en las entidades de información. Se realizó un análisis de las dimensiones que conforman el capital cultural. Se expusieron variables tales como actitudes, valores, aptitudes, capacidades, aprendizaje y cultura organizacional, que se operacionalizaron en indicadores e ítems donde se tomó como base de estudio el modelo Intellectus y los modos de identificar el capital cultural en entidades educativas y culturales. Resultados: el capital cultural se identificó a partir de elementos que tenían un carácter multidimensional y que se encontraban estrechamente relacionados. Las tres dimensiones que como aspectos esenciales del capital cultural influyeron en su creación y transmisión fueron la dimensión cognitiva, la dimensión estructural y la dimensión relacional. Estas tres dimensiones se interrelacionaron entre sí, lo que hizo posible que el capital cultural se generara, se desarrollara y se transmitiera a todos los niveles de la organización, tributando a mejorar las decisiones desde el punto de vista gerencial. Conclusión: el capital cultural se impuso como un elemento indispensable que marcó una diferencia entre las organizaciones a partir de las características de sus individuos(AU)


Objective: propose guidelines to identify cultural capital in information institutions. Methods: a descriptive study was conducted. Classic document analysis was used, as well as interviews based on the variables social climate, confidence ties, communication channels, commitment and sense of belonging, work conditions, work competences, organizational culture, cultural values, satisfaction, experience and leadership. Cultural capital definitions, perspectives and component elements were presented, as well as their importance for information institutions. An analysis was conducted of the dimensions of cultural capital. Variables such as attitudes, values, aptitudes, capacities, learning and organizational culture were operationalized through indicators and headings based on the Intellectus model and identification modes for cultural capital in educational and cultural institutions. Results: cultural capital was identified on the basis of multidimensional, closely interrelated elements. The cognitive dimension, the structural dimension and the relational dimension were found to play an essential role in the creation and transmission of cultural capital. These three dimensions were closely interrelated, making it possible for cultural capital to be generated, developed and transmitted to all levels in the organization, contributing to improve management decisions. Conclusions: cultural capital was found to be a crucial element in organizations, differentiating them on the basis of the characteristics of their members(AU)


Asunto(s)
Competencia Cultural/organización & administración , Centros de Información/organización & administración , Cultura Organizacional , Epidemiología Descriptiva
20.
Rev. cub. inf. cienc. salud ; 25(4): 397-409, oct.-dic. 2014.
Artículo en Español | LILACS | ID: lil-725108

RESUMEN

Proponer pautas para identificar el capital cultural en las entidades de información. Métodos: se realizó un estudio de tipo descriptivo. Se utilizaron el análisis documental clásico y las entrevistas a través de las variables clima social, lazos de confianza, canales de comunicación, compromiso y sentido de pertenencia, condiciones de trabajo, competencias laborales, cultura organizacional, valores culturales, satisfacción, experiencia y liderazgo. Se mostraron las definiciones, perspectivas y elementos del capital cultural y se demostró su importancia en las entidades de información. Se realizó un análisis de las dimensiones que conforman el capital cultural. Se expusieron variables tales como actitudes, valores, aptitudes, capacidades, aprendizaje y cultura organizacional, que se operacionalizaron en indicadores e ítems donde se tomó como base de estudio el modelo Intellectus y los modos de identificar el capital cultural en entidades educativas y culturales. Resultados: el capital cultural se identificó a partir de elementos que tenían un carácter multidimensional y que se encontraban estrechamente relacionados. Las tres dimensiones que como aspectos esenciales del capital cultural influyeron en su creación y transmisión fueron la dimensión cognitiva, la dimensión estructural y la dimensión relacional. Estas tres dimensiones se interrelacionaron entre sí, lo que hizo posible que el capital cultural se generara, se desarrollara y se transmitiera a todos los niveles de la organización, tributando a mejorar las decisiones desde el punto de vista gerencial. Conclusión: el capital cultural se impuso como un elemento indispensable que marcó una diferencia entre las organizaciones a partir de las características de sus individuos...


Propose guidelines to identify cultural capital in information institutions. Methods: a descriptive study was conducted. Classic document analysis was used, as well as interviews based on the variables social climate, confidence ties, communication channels, commitment and sense of belonging, work conditions, work competences, organizational culture, cultural values, satisfaction, experience and leadership. Cultural capital definitions, perspectives and component elements were presented, as well as their importance for information institutions. An analysis was conducted of the dimensions of cultural capital. Variables such as attitudes, values, aptitudes, capacities, learning and organizational culture were operationalized through indicators and headings based on the Intellectus model and identification modes for cultural capital in educational and cultural institutions. Results: cultural capital was identified on the basis of multidimensional, closely interrelated elements. The cognitive dimension, the structural dimension and the relational dimension were found to play an essential role in the creation and transmission of cultural capital. These three dimensions were closely interrelated, making it possible for cultural capital to be generated, developed and transmitted to all levels in the organization, contributing to improve management decisions. Conclusions: cultural capital was found to be a crucial element in organizations, differentiating them on the basis of the characteristics of their members...


Asunto(s)
Humanos , Centros de Información/organización & administración , Competencia Cultural/organización & administración , Cultura Organizacional , Epidemiología Descriptiva
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