Enriched high purity germanium detectors for the LEGEND-200 experiment: purification and characterization by quadrupole and high resolution inductively coupled plasma mass spectrometry (ICP-MS).

LEGEND-200 (Large Enriched Germanium Experiment for Neutrinoless ßß Decay) is a physics experiment at the Gran Sasso National Laboratories (LNGS) in Italy searching for neutrinoless double beta (0υßß) decay of 76Ge using enriched high purity germanium (HPGe) detectors with a total mass of about 200 kg. During the production of germanium crystals, especially during the crystal cutting, a fraction of the enriched germanium remains as metal residues. To reuse these residual materials again for crystal growing, they must be efficiently purified. A special plant was built to purify and convert Ge metal to GeO2. Quadrupole ICP-MS (Q-ICP-MS) and High Resolution ICP-MS (HR-ICP-MS) were used to characterize the starting materials, reaction and final products. The results of the analyses are presented here.

Characterization of a kg-scale archaeological lead-based PbWO4 cryogenic detector for the RES-NOVA experiment.

Core-collapse Supernovae (SNe) are one of the most energetic events in the Universe, during which almost all the star's binding energy is released in the form of neutrinos. These particles are direct probes of the processes occurring in the stellar core and provide unique insights into the gravitational collapse. RES-NOVA will revolutionize how we detect neutrinos from astrophysical sources, by deploying the first ton-scale array of cryogenic detectors made from archaeological lead. Pb offers the highest neutrino interaction cross-section via coherent elastic neutrino-nucleus scattering (CEνNS). Such process will enable RES-NOVA to be equally sensitive to all neutrino flavours. For the first time, we propose the use archaeological Pb as sensitive target material in order to achieve an ultra-low background level in the region of interest (O(1 keV)). All these features make possible the deployment of the first cm-scale neutrino telescope for the investigation of astrophysical sources. In this contribution, we will characterize the radiopurity level and the performance of a small-scale proof-of-principle detector of RES-NOVA, consisting in a PbWO4 crystal made from archaeological-Pb operated as cryogenic detector.

Secular equilibrium assessment in a CaWO4 target crystal from the dark matter experiment CRESST using Bayesian likelihood normalisation.

CRESST is a leading direct detection sub-GeVc-2 dark matter experiment. During its second phase, cryogenic bolometers were used to detect nuclear recoils off the CaWO4 target crystal nuclei. The previously established electromagnetic background model relies on Secular Equilibrium (SE) assumptions. In this work, a validation of SE is attempted by comparing two likelihood-based normalisation results using a recently developed spectral template normalisation method based on Bayesian likelihood. Albeit we find deviations from SE in some cases we conclude that these deviations are artefacts of the fit and that the assumptions of SE is physically meaningful.

Geant4-based electromagnetic background model for the CRESST dark matter experiment.

The CRESST (Cryogenic Rare Event Search with Superconducting Thermometers) dark matter search experiment aims for the detection of dark matter particles via elastic scattering off nuclei in CaWO 4 crystals. To understand the CRESST electromagnetic background due to the bulk contamination in the employed materials, a model based on Monte Carlo simulations was developed using the Geant4 simulation toolkit. The results of the simulation are applied to the TUM40 detector module of CRESST-II phase 2. We are able to explain up to ( 68 ± 16 ) % of the electromagnetic background in the energy range between 1 and 40 keV .

Limits on Momentum-Dependent Asymmetric Dark Matter with CRESST-II.

The usual assumption in direct dark matter searches is to consider only the spin-dependent or spin-independent scattering of dark matter particles. However, especially in models with light dark matter particles O(GeV/c^{2}), operators which carry additional powers of the momentum transfer q^{2} can become dominant. One such model based on asymmetric dark matter has been invoked to overcome discrepancies in helioseismology and an indication was found for a particle with a preferred mass of 3 GeV/c^{2} and a cross section of 10^{-37} cm^{2}. Recent data from the CRESST-II experiment, which uses cryogenic detectors based on CaWO_{4} to search for nuclear recoils induced by dark matter particles, are used to constrain these momentum-dependent models. The low energy threshold of 307 eV for nuclear recoils of the detector used, allows us to rule out the proposed best fit value above.

Low-level γ-ray spectrometry at the underground laboratory Garching.

Two low-background setups for material screening based on HPGe detectors were built in the Garching Underground Laboratory with an overburden of ~10 m.w.e. They include several layers of passive shielding as well as an active muon veto. The first setup (GEM) comprises a 150% efficiency HPGe detector which can optionally be surrounded by a NaI(Tl) scintillation detector that serves as anti-Compton veto. The second setup (LoAx) consists of two smaller HPGe detectors which are arranged face-to-face to cover a larger solid angle around the sample and to allow coincidence measurements. For a 5.6 kg piece of copper after 11 days of measurement we have reached a sensitivity for (226)Ra and (228)Ra/(228)Th of ~5 mBq kg(-1) with the GEM setup. In the LoAx setup we have achieved limits of less than 100 mBq kg(-1) for (234)Th and (210)Pb with a 156 g sample of PPO wavelength shifter after 18 days of measurement.

Primary unilateral fibroleiomyoma of the ovary in a pregnant mare: a case report.

Fibroleiomyomas of the ovary are rare. This smooth muscle tumour may develop and increase in size during pregnancy. Diagnosis is based on histological and immunohistochemical analyses. Histologically, the ovarian fibroleiomyoma consists of non-invasive bundles of smooth muscle cells that synthesize collagen-like extracellular matrix. This case report describes an ovarian fibroleiomyoma in a pregnant multiparous broodmare with a history of intermittent signs of colic.

Properties of maltose-inducible alpha-glucosidase MalL (sucrase-isomaltase-maltase) in Bacillus subtilis: evidence for its contribution to maltodextrin utilization.

Recently, we identified the maltose inducible alpha-glucosidase MalL of Bacillus subtilis. The malL gene encodes a 561-residue protein with amino acid identities to several alpha-glucosidases and is located in a nine-gene spanning gene cluster, which is presumably organized in an operon. MalL was overproduced, purified, and its enzymatic characteristics were described in more detail. This characterization of the enzyme showed a protein stable up to 37 degrees C after temperature treatment for 15 min and exhibiting an optimal reaction temperature of 42 degrees C. Various disaccharides such as sucrose, maltose, and isomaltose were hydrolyzed with different efficiencies. MalL also hydrolyzes longer maltodextrins from maltotriose up to maltohexaose, but not maltoheptaose, palatinose, isomaltotriose, or isomaltotetraose. MalL expression is subject to both maltose induction and carbon catabolite repression. In this article, we present data demonstrating that induction of MalL expression also occurs when starch, amylose, or glycogen are present in the growth medium. The hydrolysis of these substrates by alpha-amylase presumably leads to products which, when taken up into the cytoplasm, trigger the initiation of maltose operon transcription. Furthermore, MalL expression varies temporally, showing a second induction in the stationary growth phase.

Identification and enzymatic characterization of the maltose-inducible alpha-glucosidase MalL (sucrase-isomaltase-maltase) of Bacillus subtilis.

A gene coding for a putative alpha-glucosidase has been identified in the open reading frame yvdL (now termed malL), which was sequenced as part of the Bacillus subtilis genome project. The enzyme was overproduced in Escherichia coli and purified. Further analyses indicate that MalL is a specific oligo-1,4-1,6-alpha-glucosidase (sucrase-maltase-isomaltase). MalL expression in B. subtilis requires maltose induction and is subject to carbon catabolite repression by glucose and fructose. Insertional mutagenesis of malL resulted in a complete inactivation of the maltose-inducible alpha-glucosidase activity in crude protein extracts and a Mal- phenotype.