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1.
J Agric Food Chem ; 72(20): 11617-11628, 2024 May 22.
Artículo en Inglés | MEDLINE | ID: mdl-38728580

RESUMEN

When grapes are exposed to wildfire smoke, certain smoke-related volatile phenols (VPs) can be absorbed into the fruit, where they can be then converted into volatile-phenol (VP) glycosides through glycosylation. These volatile-phenol glycosides can be particularly problematic from a winemaking standpoint as they can be hydrolyzed, releasing volatile phenols, which can contribute to smoke-related off-flavors. Current methods for quantitating these volatile-phenol glycosides present several challenges, including the requirement of expensive capital equipment, limited accuracy due to the molecular complexity of the glycosides, and the utilization of harsh reagents. To address these challenges, we proposed an enzymatic hydrolysis method enabled by a tailored enzyme cocktail of novel glycosidases discovered through genome mining, and the generated VPs from VP glycosides can be quantitated by gas chromatography-mass spectrometry (GC-MS). The enzyme cocktails displayed high activities and a broad substrate scope when using commercially available VP glycosides as the substrates for testing. When evaluated in an industrially relevant matrix of Cabernet Sauvignon wine and grapes, this enzymatic cocktail consistently achieved a comparable efficacy of acid hydrolysis. The proposed method offers a simple, safe, and affordable option for smoke taint analysis.


Asunto(s)
Frutas , Cromatografía de Gases y Espectrometría de Masas , Glicósido Hidrolasas , Glicósidos , Fenoles , Humo , Vitis , Hidrólisis , Glicósidos/química , Glicósidos/metabolismo , Glicósidos/análisis , Humo/análisis , Glicósido Hidrolasas/metabolismo , Glicósido Hidrolasas/química , Glicósido Hidrolasas/genética , Fenoles/química , Fenoles/metabolismo , Vitis/química , Frutas/química , Frutas/enzimología , Vino/análisis , Incendios Forestales , Biocatálisis
2.
J Agric Food Chem ; 72(5): 2697-2707, 2024 Feb 07.
Artículo en Inglés | MEDLINE | ID: mdl-38054424

RESUMEN

There is a need for transformational innovation within the existing food system to achieve United Nations Sustainable Development Goal 2 of ending hunger within a sustainable agricultural system by 2030. Mycelium, the vegetative growth form of filamentous fungi, may represent a convergence of several features crucial for the development of food products that are nutritious, desirable, scalable, affordable, and environmentally sustainable. Mycelium has gained interest as technology advances demonstrate its ability to provide scalable biomass for food production delivering good flavor and quality protein, fiber, and essential micronutrients urgently needed to improve public health. We review the potential of mycelium as an environmentally sustainable food to address malnutrition and undernutrition, driven by food insecurity and caloric dense diets with less than optimal macro- and micronutrient density.


Asunto(s)
Promoción de la Salud , Desnutrición , Humanos , Hambre , Micronutrientes , Micelio , Abastecimiento de Alimentos
3.
J Agric Food Chem ; 71(28): 10543-10564, 2023 Jul 19.
Artículo en Inglés | MEDLINE | ID: mdl-37428126

RESUMEN

There is an urgent requirement to minimize food waste and create more sustainable food systems that address global increases in malnutrition and hunger. The nutritional value of brewers' spent grain (BSG) makes it attractive for upcycling into value-added ingredients rich in protein and fiber having a lower environmental impact than comparable plant-based ingredients. BSG is predictably available in large quantities globally and can therefore play a role in addressing hunger in the developing world via the fortification of humanitarian food aid products. Moreover, addition of BSG-derived ingredients can improve the nutritional profile of foods commonly consumed in more developed regions, which may aid in reducing the prevalence of dietary-related disease and mortality. Challenges facing the widespread utilization of upcycled BSG ingredients include regulatory status, variability of raw material composition, and consumer perception as low-value waste products; however, the rapidly growing upcycled food market suggests increasing consumer acceptability and opportunities for significant market growth via effective new product innovation and communication strategies.


Asunto(s)
Desnutrición , Eliminación de Residuos , Alimentos , Antioxidantes/análisis , Dieta Vegetariana , Grano Comestible/química
4.
J Agric Food Chem ; 70(48): 15007-15027, 2022 Dec 07.
Artículo en Inglés | MEDLINE | ID: mdl-36409321

RESUMEN

Research continues to provide compelling insights into potential health benefits associated with diets rich in plant-based natural products (PBNPs). Coupled with evidence from dietary intervention trials, dietary recommendations increasingly include higher intakes of PBNPs. In addition to health benefits, PBNPs can drive flavor and sensory perceptions in foods and beverages. Chardonnay marc (pomace) is a byproduct of winemaking obtained after fruit pressing that has not undergone fermentation. Recent research has revealed that PBNP diversity within Chardonnay marc has potential relevance to human health and desirable sensory attributes in food and beverage products. This review explores the potential of Chardonnay marc as a valuable new PBNP ingredient in the food system by combining health, sensory, and environmental sustainability benefits that serves as a model for development of future ingredients within a sustainable circular bioeconomy. This includes a discussion on the potential role of computational methods, including artificial intelligence (AI), in accelerating research and development required to discover and commercialize this new source of PBNPs.


Asunto(s)
Inteligencia Artificial , Industria de Alimentos , Humanos
5.
Front Microbiol ; 11: 393, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32318028

RESUMEN

Food and human health are inextricably linked. As such, revolutionary impacts on health have been derived from advances in the production and distribution of food relating to food safety and fortification with micronutrients. During the past two decades, it has become apparent that the human microbiome has the potential to modulate health, including in ways that may be related to diet and the composition of specific foods. Despite the excitement and potential surrounding this area, the complexity of the gut microbiome, the chemical composition of food, and their interplay in situ remains a daunting task to fully understand. However, recent advances in high-throughput sequencing, metabolomics profiling, compositional analysis of food, and the emergence of electronic health records provide new sources of data that can contribute to addressing this challenge. Computational science will play an essential role in this effort as it will provide the foundation to integrate these data layers and derive insights capable of revealing and understanding the complex interactions between diet, gut microbiome, and health. Here, we review the current knowledge on diet-health-gut microbiota, relevant data sources, bioinformatics tools, machine learning capabilities, as well as the intellectual property and legislative regulatory landscape. We provide guidance on employing machine learning and data analytics, identify gaps in current methods, and describe new scenarios to be unlocked in the next few years in the context of current knowledge.

6.
J AOAC Int ; 96(4): 705-11, 2013.
Artículo en Inglés | MEDLINE | ID: mdl-24000740

RESUMEN

An international collaborative study was conducted on an HPLC method with fluorescent detection for the determination of flavanols and procyanidins in chocolate and cocoa-containing materials. The sum of the oligomeric fractions with degree of polymerization 1-10 was the determined content value. Sample materials included dark and milk chocolates, cocoa powder, cocoa liquors, and cocoa extracts. The content ranged from approximately 2 to 500 mg/g (defatted basis). Thirteen laboratories--representing commercial, industrial, and academic institutions in six countries--participated in this interlaboratory study. Fourteen samples were sent as blind duplicates to the collaborators. Results for 12 laboratories yielded repeatability RSD (RSDr) values below 10% for all materials analyzed, ranging from 4.17 to 9.61%. Reproducibility RSD (RSDR) values ranged from 5.03 to 12.9% for samples containing 8.07 to 484.7 mg/g material analyzed. In one sample containing a low content of flavanols and procyanidins (approximately 2 mg/g), the RSDR was 17.68%.


Asunto(s)
Biflavonoides/análisis , Cacao/química , Catequina/análisis , Flavonoides/análisis , Extractos Vegetales/análisis , Proantocianidinas/análisis , Cromatografía Líquida de Alta Presión , Polvos , Reproducibilidad de los Resultados
7.
J AOAC Int ; 95(4): 1153-60, 2012.
Artículo en Inglés | MEDLINE | ID: mdl-22970585

RESUMEN

An international collaborative study was conducted on an HPLC method with fluorescent detection (FLD) for the determination of flavanols and procyanidins in materials containing chocolate and cocoa. The sum of the oligomeric fractions with degree of polymerization 1-10 was the determined content value. Sample materials included dark and milk chocolates, cocoa powder, cocoa liquors, and cocoa extracts. The content ranged from approximately 2 to 500 mg/g (defatted basis). Thirteen laboratories representing commercial, industrial, and academic institutions in six countries participated in the study. Fourteen samples were sent as blind duplicates to the collaborators. Results from 12 laboratories yielded repeatability relative standard deviation (RSDr) values that were below 10% for all materials analyzed, ranging from 4.17 to 9.61%. The reproducibility relative standard deviation (RSD(R)) values ranged from 5.03 to 12.9% for samples containing 8.07 to 484.7 mg/g. In one sample containing a low content of flavanols and procyanidins (approximately 2 mg/g), the RSD(R) was 17.68%. Based on these results, the method is recommended for Official First Action for the determination of flavanols and procyanidins in chocolate, cocoa liquors, powder(s), and cocoa extracts.


Asunto(s)
Biflavonoides/análisis , Cacao/metabolismo , Catequina/análisis , Técnicas de Química Analítica/métodos , Técnicas de Química Analítica/normas , Cromatografía Líquida de Alta Presión/métodos , Flavanonas/análisis , Análisis de los Alimentos/métodos , Análisis de los Alimentos/normas , Proantocianidinas/análisis , Cooperación Internacional , Laboratorios , Lípidos/análisis , Modelos Químicos , Polimerizacion , Polvos/análisis , Estándares de Referencia , Reproducibilidad de los Resultados
9.
Mol Aspects Med ; 31(6): 546-57, 2010 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-20854838

RESUMEN

Data on the potential health benefits of dietary flavanols and procyanidins, especially in the context of cardiovascular health, are considerable and continue to accumulate. Significant progress has been made in flavanol analytics and the creation of phytonutrient-content food databases, and novel data emanated from epidemiological investigations as well as dietary intervention studies. However, a comprehensive understanding of the pharmacological properties of flavanols and procyanidins, including their precise mechanisms of action in vivo, and a conclusive, consensus-based accreditation of a causal relationship between intake and health benefits in the context of primary and secondary cardiovascular disease prevention is still outstanding. Thus, the objective of this review is to identify and discuss key questions and gaps that will need to be addressed in order to conclusively demonstrate whether or not dietary flavanols and procyanidins have a role in preventing, delaying the onset of, or treating cardiovascular diseases, and thus improving human life expectancy and quality of life.


Asunto(s)
Enfermedades Cardiovasculares/prevención & control , Dieta , Flavonoides/administración & dosificación , Proantocianidinas/administración & dosificación , Predicción , Conductas Relacionadas con la Salud , Conocimientos, Actitudes y Práctica en Salud , Directrices para la Planificación en Salud , Humanos
10.
Exp Biol Med (Maywood) ; 232(2): 293-300, 2007 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-17259337

RESUMEN

There has been considerable work on the relationships between nutrition and the immune response, particularly on studies that have focused on adaptive responses. There is increasing recognition of the importance of innate immunity in host protection and initiation of cytokine networks. In this study, we examined the effect of select cocoa flavanols and procyanidins on innate responses in vitro. Peripheral blood mono-nuclear cells (PBMCs), as well as purified monocytes and CD4 and CD8 T cells, were isolated from healthy volunteers and cultured in the presence of cocoa flavanol fractions that differ from another by the degree of flavanol polymerization: short-chain flavanol fraction (SCFF), monomers to pentamers; and long-chain flavanol fraction (LCFF), hexamers to decamers. Parallel investigations were also done with highly purified flavanol monomers and procyanidin dimers. The isolated cells were then challenged with lipopolysaccharide (LPS) with quantitation of activation using CD69 and CD83 expression and analysis of secreted tumor necrosis factor (TNF)-alpha, interleukin (IL)-1beta, IL-6, IL-10, and granulocyte macrophage colony-stimulating factor (GM-CSF). The chain length of flavanol fractions had a significant effect on cytokine release from both unstimulated and LPS-stimulated PBMCs. For example, there was a striking increase of LPS-induced synthesis of IL-1beta, IL-6, IL-10, and TNF-alpha in the presence of LCFF. LCFF and SCFF, in the absence of LPS, stimulated the production of GM-CSF. In addition, LCFF and SCFF increased expression of the B cell markers CD69 and CD83. There were also unique differential responses in the mononuclear cell populations studied. We conclude that the oligomers are potent stimulators of both the innate immune system and early events in adaptive immunity.


Asunto(s)
Biflavonoides/farmacología , Biopolímeros/farmacología , Linfocitos T CD4-Positivos/efectos de los fármacos , Linfocitos T CD8-positivos/efectos de los fármacos , Cacao/química , Catequina/farmacología , Proantocianidinas/farmacología , Adulto , Biflavonoides/química , Biopolímeros/química , Linfocitos T CD4-Positivos/inmunología , Linfocitos T CD4-Positivos/metabolismo , Linfocitos T CD8-positivos/inmunología , Linfocitos T CD8-positivos/metabolismo , Catequina/química , Proliferación Celular , Cromatografía Líquida de Alta Presión , Citocinas/metabolismo , Humanos , Proantocianidinas/química
11.
J Cardiovasc Pharmacol ; 47 Suppl 2: S103-9; discussion 119-21, 2006.
Artículo en Inglés | MEDLINE | ID: mdl-16794446

RESUMEN

A low sodium diet has often been implicated in the protection of low blood pressure populations from hypertension, but several other dietary factors, including those as yet unidentified, may also be involved. The Kuna Indians of Panama are free of hypertension and cardiovascular disease, but this is changing with migration to urban areas. We compared the indigenous diet of Kuna Indians living on remote islands in Panama (Ailigandi), whose lifestyle is largely hunter-gatherer, with those who have moved to a suburb of Panama City (Vera Cruz). Between April and October 1999, members of a Kuna research team administered a 118-item food frequency questionnaire to133 adult Kuna from Ailigandi and 183 from Vera Cruz. Single 24-hour urine collections and nonfasting blood samples were obtained. The Kuna in Ailigandi reported consuming a 10-fold higher amount of cocoa-containing beverages, 4 times the amount of fish, and twice the amount of fruit as urban Kuna (P<0.05 by t test). Salt added was ample among those living in Ailigandi and Vera Cruz according to both self-report (7.1+/-1.1 and 4.6+/-0.3 tsp weekly) and urinary sodium levels (177+/-9 and 160+/-7 mEq Na/g creatinine), respectively. The low blood pressure of island-dwelling Kuna does not seem to be related to a low salt diet. Among dietary factors that varied among migrating Kuna, the notably higher intake of flavanol-rich cocoa is a potential candidate for further study.


Asunto(s)
Aculturación , Cacao , Dieta , Flavonoides , Hipertensión/etiología , Indígenas Centroamericanos , Adulto , Animales , Bebidas , Biflavonoides/administración & dosificación , Catequina/administración & dosificación , Encuestas sobre Dietas , Femenino , Peces , Flavonoides/administración & dosificación , Frutas , Humanos , Masculino , Persona de Mediana Edad , Panamá , Proantocianidinas/administración & dosificación , Sodio en la Dieta/administración & dosificación , Verduras
12.
J Cardiovasc Pharmacol ; 47 Suppl 2: S110-8; discussion S119-21, 2006.
Artículo en Inglés | MEDLINE | ID: mdl-16794447

RESUMEN

Flavanols and their related oligomeric compounds, the procyanidins, have received increased attention during the past decade due to their reported health benefits. On the basis of compelling data published during the past decade demonstrating that the consumption of certain flavanol-rich foods can improve markers of cardiovascular health, additional clinical, and epidemiological research is clearly warranted to establish appropriate public health recommendations. However, recommendations on the consumption of these foods appropriate for use by health professionals can only be made on the basis of clinical investigations that accurately identify and quantify--through proper analytical measurement systems--the flavanols in the foods used in these investigations. This manuscript provides an overview of the strengths, weaknesses, and limitations of commonly used analytical methods to characterize the content of flavanols in foods. Two nonspecific measurements widely used by investigators, the Folin-Ciocalteu assay and the Oxygen Radical Absorbance Capacity (ORAC) measurement, are discussed in this context, as is the use of various high-performance liquid chromatography methods that provide more specific data related to the content of flavanols in foods. A comparison of the data obtained from these analytical methods to those of the more rigorous high-performance liquid chromatography analyses demonstrates that these nonspecific methods are ill-suited for providing unequivocal data necessary to evaluate the importance of dietary flavanols in the context of improving cardiovascular health. Meaningful dietary recommendations for the consumption of flavanol-rich foods will only be made possible by additional well-designed clinical and epidemiological studies enabled by detailed compositional data obtained through use of appropriate analytical methods.


Asunto(s)
Flavonoides/análisis , Análisis de los Alimentos/métodos , Biflavonoides/análisis , Enfermedades Cardiovasculares/prevención & control , Catequina/análisis , Cromatografía Líquida de Alta Presión , Directrices para la Planificación en Salud , Humanos , Molibdeno , Fenoles/análisis , Proantocianidinas/análisis , Especies Reactivas de Oxígeno/análisis , Compuestos de Tungsteno
13.
J Cardiovasc Pharmacol ; 47 Suppl 2: S163-71; discussion S172-6, 2006.
Artículo en Inglés | MEDLINE | ID: mdl-16794453

RESUMEN

Signs of chronic or acute inflammation have been demonstrated in most cardiovascular diseases of multifactorial pathogenesis, including atherosclerosis and chronic heart failure. The triggers and mechanisms leading to inflammation may vary between clinical conditions but they share many common mediators, including specific patterns of eicosanoid and cytokine production. Certain cocoa-based products can be rich in a subclass of flavonoids known as flavanols, some of which have been found in model systems to possess potential anti-inflammatory activity relevant to cardiovascular health. Indeed, experimental evidence demonstrates that some cocoa-derived flavanols can reduce the production and effect of pro-inflammatory mediators either directly or by acting on signaling pathways. However, it should be noted that the evidence for any beneficial effects of cocoa flavanols in providing a meaningful anti-inflammatory action has been gathered predominantly from in vitro experiments. Therefore, additional research in well-designed human clinical experiments, using cocoa properly characterized in terms of flavanol content, would be a welcome addition to the evidence base to determine unambiguously if this benefit does indeed exist. If so, then flavanol-rich cocoa could be a potential candidate for the treatment, or possibly prevention, of the broad array of chronic diseases that are linked to dysfunctional inflammatory responses.


Asunto(s)
Antiinflamatorios/farmacología , Cacao/química , Flavonoides/farmacología , Biflavonoides/análisis , Plaquetas/efectos de los fármacos , Enfermedades Cardiovasculares/inmunología , Enfermedades Cardiovasculares/patología , Catequina/análisis , Citocinas/biosíntesis , Eicosanoides/metabolismo , Humanos , FN-kappa B/metabolismo , Óxido Nítrico/fisiología , Proantocianidinas/análisis
14.
J Cardiovasc Pharmacol ; 47 Suppl 2: S177-86; discussion S206-9, 2006.
Artículo en Inglés | MEDLINE | ID: mdl-16794456

RESUMEN

Endothelial dysfunction characterizes many disease states including subclinical atherosclerosis. The consumption of flavanol-rich cocoa and cocoa-based products has been shown to improve endothelial function in both compromised and otherwise normal, healthy individuals when administered either acutely or over a period of several days, or weeks. Women experience increased risk for cardiovascular disease after menopause, which can be associated with endothelial dysfunction. Whether a flavanol-rich cocoa-based product can improve endothelial function in hypercholesterolemic postmenopausal women is not known. The purpose of the present study was to determine whether chronic dietary administration of flavanol-rich cocoa improves endothelial function and markers of cardiovascular health in hypercholesterolemic postmenopausal women. Thirty-two postmenopausal hypercholesterolemic women were randomly assigned to consume a high-flavanol cocoa beverage (high cocoa flavanols (CF)--446 mg of total flavanols), or a low-flavanol cocoa beverage (low CF--43 mg of total flavanols) for 6 weeks in a double-blind study (n=16 per group). Endothelial function was determined by brachial artery-reactive hyperemia. Plasma was analyzed for lipids (total cholesterol, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol), hormones (follicle-stimulating hormone), total nitrate/nitrite, activation of cellular adhesion markers (vascular cell adhesion molecule 1, intercellular adhesion molecule 1, E-Selectin, P-Selectin), and platelet function and reactivity. Changes in these plasma markers were then correlated to brachial reactivity. Brachial artery hyperemic blood flow increased significantly by 76% (P<0.05 vs. baseline) after the 6-week cocoa intervention in the high CF group, compared with 32% in the low CF cocoa group (P=ns vs. baseline). The 2.4-fold increase in hyperemic blood flow with high CF cocoa closely correlated (r2=0.8) with a significant decrease (11%) in plasma levels of soluble vascular cell adhesion molecule-1. Similar responses were not observed after chronic use of low CF. There were no significant differences between high and low CF in other biochemical markers and parameters measured. This study is the first to identify beneficial vascular effects of flavanol-rich cocoa consumption in hypercholesterolemic postmenopausal women. In addition, our results suggest that reductions in plasma soluble vascular cell adhesion molecule-1 after chronic consumption of a flavanol-rich cocoa may be mechanistically linked to improved vascular reactivity.


Asunto(s)
Cacao , Moléculas de Adhesión Celular/metabolismo , Endotelio Vascular/efectos de los fármacos , Endotelio Vascular/fisiología , Flavonoides/farmacología , Hipercolesterolemia/tratamiento farmacológico , Bebidas , Plaquetas/efectos de los fármacos , Plaquetas/fisiología , Arteria Braquial/efectos de los fármacos , Arteria Braquial/fisiología , Método Doble Ciego , Femenino , Flavonoides/administración & dosificación , Humanos , Hipercolesterolemia/fisiopatología , Lípidos/sangre , Persona de Mediana Edad , Posmenopausia
15.
J Agric Food Chem ; 54(5): 1571-6, 2006 Mar 08.
Artículo en Inglés | MEDLINE | ID: mdl-16506802

RESUMEN

A new chromatographic approach for separating cacao procyanidins according to their degree of polymerization has been developed. It utilizes diol stationary phase columns operating in normal phase mode with a binary gradient of acidified acetonitrile and methanol-water. Performance of the diol stationary phase was evaluated on an analytical scale utilizing classical chromatographic conditions for the normal phase separation of procyanidins according to their degree of polymerization. The new separation approach was developed on an analytical scale but further extended to the preparative scale. These newly developed analytical and preparative high-performance liquid chromatography procedures were successfully applied to the separation, as well as isolation, of cacao procyanidins from unfermented cacao seeds. The degree of polymerization associated with each molecular weight fraction was determined by mass spectrometry.


Asunto(s)
Cacao/química , Cromatografía Líquida de Alta Presión/métodos , Polímeros/química , Proantocianidinas/química , Proantocianidinas/aislamiento & purificación , Semillas/química , Espectrometría de Masas , Peso Molecular
16.
Proc Natl Acad Sci U S A ; 103(4): 1024-9, 2006 Jan 24.
Artículo en Inglés | MEDLINE | ID: mdl-16418281

RESUMEN

Epidemiological and medical anthropological investigations suggest that flavanol-rich foods exert cardiovascular health benefits. Endothelial dysfunction, a prognostically relevant key event in atherosclerosis, is characterized by a decreased bioactivity of nitric oxide (NO) and impaired flow-mediated vasodilation (FMD). We show in healthy male adults that the ingestion of flavanol-rich cocoa was associated with acute elevations in levels of circulating NO species, an enhanced FMD response of conduit arteries, and an augmented microcirculation. In addition, the concentrations and the chemical profiles of circulating flavanol metabolites were determined, and multivariate regression analyses identified (-)-epicatechin and its metabolite, epicatechin-7-O-glucuronide, as independent predictors of the vascular effects after flavanol-rich cocoa ingestion. A mixture of flavanols/metabolites, resembling the profile and concentration of circulating flavanol compounds in plasma after cocoa ingestion, induced a relaxation in preconstricted rabbit aortic rings ex vivo, thus mimicking acetylcholine-induced relaxations. Ex vivo flavanol-induced relaxation, as well as the in vivo increases in FMD, were abolished by inhibition of NO synthase. Oral administration of chemically pure (-)-epicatechin to humans closely emulated acute vascular effects of flavanol-rich cocoa. Finally, the concept that a chronic intake of high-flavanol diets is associated with prolonged, augmented NO synthesis is supported by data that indicate a correlation between the chronic consumption of a cocoa flavanol-rich diet and the augmented urinary excretion of NO metabolites. Collectively, our data demonstrate that the human ingestion of the flavanol (-)-epicatechin is, at least in part, causally linked to the reported vascular effects observed after the consumption of flavanol-rich cocoa.


Asunto(s)
Cacao/metabolismo , Catequina/fisiología , Flavonas/química , Administración Oral , Adulto , Animales , Aorta/patología , Fenómenos Fisiológicos Cardiovasculares , Sistema Cardiovascular/metabolismo , Catequina/química , Dieta , Endotelio/metabolismo , Flavonas/metabolismo , Humanos , Cinética , Masculino , Modelos Estadísticos , Análisis Multivariante , Óxido Nítrico/metabolismo , Óxido Nítrico Sintasa/metabolismo , Conejos , Análisis de Regresión , Riesgo , Transducción de Señal , Factores de Tiempo
17.
Clin Dev Immunol ; 12(1): 11-7, 2005 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-15712594

RESUMEN

The consumption of a diet rich in certain flavonoids, including the flavanol sub-class, has been associated with a reduced risk for vascular disease. We evaluated the effects of the regular consumption (14 d) of a flavanol-containing milk chocolate (FCMC) or cocoa butter chocolate (CBC) on variables related to vascular disease risk, oxidative stress and physical activity. Twenty-eight free-living, young (18-20 years old) male soccer players consumed daily 105 g of FCMC (168 mg of flavanols) or CBC (< 5 mg of flavanols), as part of their normal diet. The consumption of FCMC was significantly associated with a decrease in diastolic blood pressure (- 5 mm Hg), mean blood pressure (- 5 mm Hg), plasma cholesterol (-11%), LDL-cholesterol (-15%), malondialdehyde (- 12%), urate (- 11%) and lactate dehydrogenase (LDH) activity (- 11%), and an increase in vitamin E/cholesterol (+ 12%). No relevant changes in these variables were associated with CBC consumption. No changes in the plasma levels of (-)-epicatechin were observed following analysis of fasting blood samples. In conclusion, FCMC consumption was associated with changes in several variables often associated with cardiovascular health and oxidant stress. The presence of significant quantities of flavanols in FCMC is likely to have been one of the contributing factors to these results.


Asunto(s)
Cacao/química , Dieta , Flavonoides/farmacología , Oxidantes/metabolismo , Estrés Oxidativo/efectos de los fármacos , Fútbol , Adolescente , Adulto , Humanos , Masculino , Fenómenos Fisiológicos de la Nutrición
18.
Clin Dev Immunol ; 12(1): 1-9, 2005 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-15712593

RESUMEN

Platelet activity and platelet-endothelial cell interactions are important in the acute development of thrombosis, as well as in the pathogenesis of cardiovascular disease. An increasing number of foods have been reported to have platelet-inhibitory actions, and research with a number of flavanol-rich foods, including, grape juice, cocoa and chocolate, suggests that these foods may provide some protection against thrombosis. In the present report, we review a series of in vivo studies on the effects of flavanol-rich cocoa and chocolate on platelet activation and platelet-dependent primary hemostasis. Consumption of flavanol-rich cocoa inhibited several measures of platelet activity including, epinephrine- and ADP-induced glycoprotein (GP) IIb/IIIa and P-Selectin expression, platelet microparticle formation, and epinephrine-collagen and ADP-collagen induced primary hemostasis. The epinephrine-induced inhibitory effects on GP IIb/IIIa and primary hemostasis were similar to, though less robust than those associated with the use of low dose (81 mg) aspirin. These data, coupled with information from other studies, support the concept that flavanols present in cocoa and chocolate can modulate platelet function through a multitude of pathways.


Asunto(s)
Plaquetas/efectos de los fármacos , Plaquetas/fisiología , Flavonoides/farmacología , Animales , Plaquetas/citología , Cacao/química , Humanos , Activación Plaquetaria/efectos de los fármacos
19.
Clin Dev Immunol ; 12(1): 35-41, 2005 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-15712597

RESUMEN

Evidence suggesting that dietary polyphenols, flavanols, and proanthocyanidins in particular offer significant cardiovascular health benefits is rapidly increasing. Accordingly, reliable and accurate methods are needed to provide qualitative and quantitative food composition data necessary for high quality epidemiological and clinical research. Measurements for flavonoids and proanthocyanidins have employed a range of analytical techniques, with various colorimetric assays still being popular for estimating total polyphenolic content in foods and other biological samples despite advances made with more sophisticated analyses. More crudely, estimations of polyphenol content as well as antioxidant activity are also reported with values relating to radical scavenging activity. High-performance liquid chromatography (HPLC) is the method of choice for quantitative analysis of individual polyphenols such as flavanols and proanthocyanidins. Qualitative information regarding proanthocyanidin structure has been determined by chemical methods such as thiolysis and by HPLC-mass spectrometry (MS) techniques at present. The lack of appropriate standards is the single most important factor that limits the aforementioned analyses. However, with ever expanding research in the arena of flavanols, proanthocyanidins, and health and the importance of their future inclusion in food composition databases, the need for standards becomes more critical. At present, sufficiently well-characterized standard material is available for selective flavanols and proanthocyanidins, and construction of at least a limited food composition database is feasible.


Asunto(s)
Flavonoides/análisis , Análisis de los Alimentos/normas , Proantocianidinas/análisis , Antioxidantes/química , Cromatografía Líquida de Alta Presión , Dimerización , Flavonoides/química , Espectrometría de Masas , Estructura Molecular , Proantocianidinas/química
20.
Am J Clin Nutr ; 81(1 Suppl): 298S-303S, 2005 01.
Artículo en Inglés | MEDLINE | ID: mdl-15640494

RESUMEN

An increasing body of epidemiologic evidence supports the concept that diets rich in fruits and vegetables can promote health and attenuate, or delay, the onset of various diseases. Epidemiologic data support the idea that these health benefits are causally linked to the consumption of certain flavonoids present in fruit and vegetables. In the context of cardiovascular health, a particular group of flavonoids, namely, the flavan-3-ols (flavanols), has received attention. Flavanol-rich, plant-derived foods and beverages include wine, tea, and various fruits and berries, as well as cocoa and cocoa products. Numerous dietary intervention studies in humans and animals indicate that flavanol-rich foods and beverages might exert cardioprotective effects with respect to vascular function and platelet reactivity. This review discusses the bioactivity of flavanols in the context of cardiovascular health, with respect to their bioavailability, their antioxidant properties, and their vascular effects.


Asunto(s)
Antioxidantes/uso terapéutico , Cacao , Enfermedades Cardiovasculares/prevención & control , Endotelio Vascular/efectos de los fármacos , Flavonoides/uso terapéutico , Proantocianidinas/uso terapéutico , Animales , Antioxidantes/farmacocinética , Disponibilidad Biológica , Plaquetas/efectos de los fármacos , Enfermedades Cardiovasculares/etiología , Endotelio Vascular/fisiología , Flavonoides/farmacocinética , Frutas , Homeostasis/efectos de los fármacos , Humanos , Proantocianidinas/farmacocinética , Ratas , Verduras
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