RESUMEN
Chenuda virus (CNUV) (Orbivirus, Reoviridae) is the only known orbivirus associated with argas (Argasidae) ticks. Scientific study of this group is necessary for understanding of Orbivirus genus evolution patterns. We conducted a comparative analysis of full genomes of five different viruses of Chenuda virus species, including Baku virus strains (BAKV) circulating in a rather limited area in the Central Asia and Transcaucasia. It was shown that VP4(OC1) and VP6(Hel) proteins variability greatly exceeds the variability of other proteins. The divergence between CNUV and BAKV in this proteins is about 50%. Even in closely related strains isolated from the same geographical region, the conservative genes of which are 90-95% identical, the VP4(OC1) and VP6(Hel) divergence reaches values that would usually be indicative of different serotypes (74.1-82.2%).
RESUMEN
Full-length genome of the Chim virus (CHIMV) (strain LEIV-858Uz) was sequenced using the next-generation sequencing approach (ID GenBank: KF801656). The CHIMV/LEIV-858Uz was isolated from the Ornithodoros tartakovskyi Olenev, 1931 ticks collected in the great gerbil (Rhombomys opimus Lichtenstein, 1823) burrow in Uzbekistan near Chim town (Kashkadarinsky region) in July of 1971. Later, four more CHIMV strains were isolated from the O. tartakovskyi, O. papillipes Birula, 1895, Rhipicephalus turanicus Pomerantsev, 1936 collected in the great gerbil burrows in Kashkadarinsky, Bukhara, and Syrdarya regions of Uzbekistan, and three strains--from the Hyalomma asiaticum Schulze et Schlottke, 1930 from the great gerbil burrows in Dzheskazgan region of Kazakhstan. The virus is a potential pathogen of humans and camels. The phylogenetic analysis revealed that the CHIMV is a novel member of the Nairovirus genus (Bunyaviridae) and closely related to the Qalyub virus (QYBV), which is prototype for the group of the same name. The amino acid homology between the CHIMV and QYBV is 87% for the RdRp catalytic center (L-segment) that is coincident with both QYBV and CHIMV associated with the Ornithodoros ticks and burrow of rodents as well. The CHIMV homologies with other nairoviruses are 30-40% for the amino acid sequences of precursor polyprotein GnGc (M-segment), whereas 50%--for the nucleocapsid N (S-segment). The data obtained permit to classify the CHIMV as a member of the QYBV group in the genus of Nairovirus (Bunyaviridae).
Asunto(s)
Argasidae/virología , Infecciones por Bunyaviridae/veterinaria , Genoma Viral , Gerbillinae/virología , Ixodes/virología , Nairovirus/clasificación , Filogenia , Enfermedades de los Roedores/virología , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Infecciones por Bunyaviridae/virología , Gerbillinae/parasitología , Kazajstán , Datos de Secuencia Molecular , Nairovirus/genética , Nairovirus/aislamiento & purificación , ARN Polimerasa Dependiente del ARN/genética , Homología de Secuencia de Aminoácido , UzbekistánRESUMEN
Full-length genomes of the Sakhalin virus (SAKH) and Paramushir virus (PRMV) (Sakhalin group, Nairovirus, Bunyaviridae) isolated from the ticks Ixodes uriae White 1852 were sequenced using the next-generation sequencing (Genbank ID: KF801659, KF801656). SAKV and PRMV have 81% identity for the part of RNA-dependent RNA-polymerase (RdRp) on the nucleotide level and 98.5% on the amino acid level. Full-length genome comparison shows that SAKV have, in average, from 25% (N-protein, S-segment) to 50% (RdRp, L-segment) similarity with the nairoviruses. The maximum value of the amino acid similarity (50.3% for RdRp) SAKV have with the Crimean-Congo hemorrhagic fever virus (CCHFV) and Dugbe virus (DUGV), which are also associated with the Ixodidae ticks. Another virus studied is Rukutama virus (RUKV) (isolated from ticks I. signatus Birulya, 1895) that recently was classified (based on morphology and antigenic reaction) to the Nairovirus genus, presumably to the Sakhalin group. In this work the genome of the RUKV was sequenced (KF892052-KF892054) and RUKV was classified as a member of the Uukuniemi group (Phlebovirus, Bunyaviridae). RUKV is closely related (93.0-95.5% similarity) with our previously described Komandory virus (KOMV). RUKV and KOMV form separate phylogenetic line neighbor of Manawa virus (MWAV) isolated from the ticks Argas abdussalami Hoogstraal et McCarthy, 1965 in Pakistan. The value of the similarity between RUCV and MWAV is 65-74% on the amino acid level.
Asunto(s)
Enfermedades de las Aves/virología , Aves/virología , Infecciones por Bunyaviridae/veterinaria , Genoma Viral , Ixodes/virología , Nairovirus/genética , Phlebovirus/genética , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Aves/parasitología , Infecciones por Bunyaviridae/virología , Datos de Secuencia Molecular , Nairovirus/clasificación , Nairovirus/aislamiento & purificación , Océano Pacífico , Phlebovirus/clasificación , Phlebovirus/aislamiento & purificación , Filogenia , Federación de Rusia , Homología de Secuencia de AminoácidoRESUMEN
The results of the virological identification of the Chikungunya fever case in Moscow (September, 2013) in an Indonesian visitor are presented. The clinic, electron microscopy, and molecular genetic data are discussed. The Ghikungunya virus (CHIKV) strain CHIKVILEIV-Moscow/1/2013 belonging to the Asian genotype (ID GenBank KF872195) was deposited into the Russian State Collection of viruses (GKV 1239; 18.11.2013).
Asunto(s)
Infecciones por Alphavirus/diagnóstico , Virus Chikungunya/genética , Infecciones por Alphavirus/patología , Infecciones por Alphavirus/virología , Secuencia de Bases , Fiebre Chikungunya , Virus Chikungunya/clasificación , Virus Chikungunya/aislamiento & purificación , Humanos , Indonesia , Masculino , Persona de Mediana Edad , Datos de Secuencia Molecular , Moscú , Filogenia , ViajeRESUMEN
The Artashat virus (ARTSV) was originally isolated fom the Ornithodoros alactagalis Issaakjan, 1936 (Argasidae Koch, 1844), which were collected in the burrow of small five-toed jerboa (Allactaga elater Lichtenstein, 1825) in Armenia in 1972. Later, the ARTSV was isolated from the O. verrucosus Olenev, Sassuchin et Fenuk, 1934 collected in the burrows of Persian gerbil (Meriones persicus Blanford, 1875) in Azerbaijan. Based on the virion morphology, the ARTSV was assigned to the Bunyaviridae viruses. In this work, the ARTSV genome was partially sequenced (GenBank ID: KF801650) and it was shown that the ARTSV is a new member of the Nairovirus genus. ARTSV has from 42% (Issyk-Kul virus) to 58% (Raza virus, Hughes group) similarity with the nairoviruses for nucleotide sequence of part of RNA-dependent RNA-polymerase (RdRp). The similarity on the amino acid level is 65-70%. Low level of homology and the equidistant position of the ARTSV on phylogenetic tree indicate that the ARTSV is a new prototype species of the Nairovirus genus (Bunyaviridae) forming a separate phylogenetic branch.
Asunto(s)
Argasidae/virología , Infecciones por Bunyaviridae/veterinaria , Genoma Viral , Gerbillinae/virología , Nairovirus/clasificación , Ornithodoros/virología , Filogenia , Enfermedades de los Roedores/virología , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Infecciones por Bunyaviridae/virología , Gerbillinae/parasitología , Datos de Secuencia Molecular , Nairovirus/genética , Nairovirus/aislamiento & purificación , ARN Polimerasa Dependiente del ARN/genética , Homología de Secuencia de Aminoácido , TranscaucasiaRESUMEN
Complete genome sequences were obtained for the LEIV-13841Ka (ID GenBank KF767463-65) and LEIV-279Az (ID GenBank KF767460-62) virus strains, which were classified as different strains of the Zaliv Terpeniya virus (ZTV). LEIV-13841Ka was isolated from the ticks Ixodes (Ceratixodes) uriae White, 1852 collected on Ariy Kamen (Commander Islands) in 1986. LEIV-279Az was isolated from the mosquitoes Culex modestus Ficalbi, 1889, collected in heron colony (Ardea Linnaeus, 1758) in Azerbaijan (1969) and was initially identified as Uukuniemi virus (UUKV). According to the results obtained LEIV-279Az is ZTV strain as well. LEIV-13841Ka and LEIV-279Az RdRp sequences have high level of homology (99%) with previously sequenced ZTV/LEIV-271Ka. The L-segment nucleotide sequences are homological with ZTV/LEIV-271Ka on the level of 94% and 98% for LEIV-13841Ka and LEIV-279Az, respectively; M-segment--89% and 88%, respectively. Such homologies for the amino acid sequences of Gn/Gc polyprotein are 98.3% and 97.7%. NP proteins of ZTV/LEIV-13841Ka and LEIV-279Az have 88.7% and 84.6% homologies with ZTV/LEIV-271Ka both for amino acid and nucleotide sequences, respectively. Thus, for the very first time we demonstrated ZTV strain isolated from mosquitoes in subtropical Transcaucasia zone. Obtained results permit to expand suggested areal of ZTV and to fill up data upon the ecology of the Uukuniemi virus group.
Asunto(s)
Antígenos Virales/genética , Aves/parasitología , Evolución Molecular , Ixodes/virología , Phlebovirus/genética , Filogenia , Animales , Asia del Norte , ADN Viral/genética , Europa (Continente) , Phlebovirus/aislamiento & purificación , Análisis de Secuencia de ADNRESUMEN
Genetic research into the Tyuleniy virus (TYUV) (ID GenBank KF815939) isolated in high latitudes from the Ixodes uriae White, 1852, ticks collected in the nesting colonies of the Alcidae (Leach, 1820) birds and Kama virus (KAMV) (ID GenBank KF815940) isolated from the I. lividus ticks collected in the nesting bird colonies in the middle part of the Russian Plane was carried out. Full-genome comparative analysis revealed 70% homology between KAMV and TYUV on the nucleotide level and 74% on the amino acid level. Thus, KAMV is a new member of the TYUV complex belonging to the seabird tick-borne virus group (STBVG) of Flavivirus (Flaviviridae). KAMV is a separate virus and forms separate phylogenetic line together with the TYUV, Meaban virus (MEAV), and Saumarez Reef virus (SREV).
Asunto(s)
Antígenos Virales/genética , Aves/parasitología , Flavivirus/genética , Ixodes/virología , Filogenia , Phocidae/virología , Animales , Secuencia de Bases , Europa (Continente) , Flavivirus/aislamiento & purificación , Datos de Secuencia Molecular , Federación de RusiaRESUMEN
Full-genome sequencing of the Caspiy virus (CASV - Caspiy virus) (ID GenBank KF801658) revealed its attribution to the Nairovirus genus of the Bunyaviridae family as a separate species. CASV forms separate line, which is the most close to the Hughes virus (HUGV) and Sakhalin virus (SAKV) groups containing viruses linked with seabirds and ticks parasitizing on them and distributed over the shelf and island ecosystems in the Northern Eurasia, as well as the North and South America.
Asunto(s)
Argasidae/virología , Aves/parasitología , Nairovirus/genética , Filogenia , Animales , Asia del Norte , Secuencia de Bases , Datos de Secuencia Molecular , Nairovirus/clasificación , Nairovirus/aislamiento & purificaciónRESUMEN
Complete genome sequencing of the Sokuluk virus (SOKV) isolated in Kyrgyzstan from bats Vespertilio pipistrellus and their obligatory parasites--Argasidae Koch, 1844, ticks was carried out. SOKV was classified as attributed to the Flaviviridae family, Flavivirus genus. The maximum homology (71% for nucleotide and 79% for amino acid sequences) was detected with respect to the Entebbe bat virus (ENTV). ENTV and SOKV form a group joining to the yellow fever virus (YFV) within the limits of the mosquito flavivirus branch. Close relation of SOKV with bat covers and human housings permits to assume SOKV potentially patogenic to human health.
Asunto(s)
Argasidae/virología , Aves/virología , Quirópteros/virología , Flavivirus/clasificación , Flavivirus/genética , Animales , Secuencia de Bases , Flavivirus/metabolismo , Humanos , KirguistánRESUMEN
Complete genome sequencing of three Tamdy (TAMV) virus strains was carried out. The prototype strain TAMV/LEIV-1308Uz was isolated for the very first time from the Hyalomma asiaticum asiaticum Schülce et Schlottke, 1929 (Ixodidae, Hyalomminae) collected in the August 1971 from sheep in the arid area near Namdybulak town (41 degrees 36' N, 64 degrees 39' E) in the Tamdinsky district of the Bukhara region (Uzbekistan). TAMV was revealed to be a prototype member of the new phylogenetic group within the limits of the Nairovirus. The TAMV homology for RdRp (L-segment) amino acid sequence is not less than 40% with Crimea-Congo hemorrhagic fever virus (CCHFV), Hazara virus (HAZV), and Dugbe virus (DUGV), which are also linked with Ixodidae ticks. The TAMV homologies with the Issyk-Kul virus (ISKV) and Caspiy virus (CASV) for RdRp are 37.6% and 37.7%, respectively. These data conformed to the low values of GnGc (M-segment) and nucleocapsid protein N (S-segment) homology. The TAMV homologies with the nairoviruses for GnGc is in average 25%; with the nairoviruses linked with Ixodidae ticks (CCHFV, DUGV, HAZV) - 33%; with Argasidae ticks (ISKV, CASV) - 28%. The TAMV/LEIV-1308Uz, LEIV-6158Ar, and LEIV-10226Az have high level of identity. The TAMV/LEIV-10226Az from Azerbaijan has 99% homology for both nucleotide and amino acid sequences of the prototype TAMV/LEIV-1308Uz RdRp. The TAMV/LEIV-6158Ar from Armenia is more divergent and has 94.2% and 96.3% homologies with the TAMV/LEIV-1308Uz, respectively. The homology between the TAMV/LEIV-1308Uz and TAMV/LEIV-10226Az for GnGc is 93%. The TAMV/LEIV-6158Ar has 90% homology for this protein with the TAMV/LEIV-1308Uz and 93% with the TAMV/LEIV-10226Az, respectively. Differences in nucleocapsid protein between three TAMV strains are 5-7%.
Asunto(s)
Bunyaviridae/clasificación , Bunyaviridae/genética , Genoma Viral , Animales , Bunyaviridae/patogenicidad , Clasificación , Humanos , Medio Oriente , Filogenia , Análisis de Secuencia de ADN , Garrapatas/genética , Garrapatas/virologíaRESUMEN
Molecular-genetic characteristics of the Okhotskiy virus (OKHV) and Aniva virus (ANIV) were studied (ID GenBank KF981623-32). These viruses are distributed over the shelf and Island areas in the high latitudes in the Okhotsk, Bering, and Barents seas and linked with nesting colonies of Alcidae seabirds and their obligatory parasites, the Ixodes uriae (Ixodidae) ticks. OKHV and ANIV are observed to be independent species within the limits of the Great Island virus (GIV) group of the Orbivirus genus of the Reoviridae family. The majority of the genes of OKHV and ANIV have high homology (VP1 - 96%, T2 - 99%, VP7 (T13) - 98%, NS1 - 94%, NS2 - 98%, NS3 - 72%, VP6 - 93%). Nevertheless, the envelope proteins containing the main specific antigenic determinants (VP2 and VP5) of OKHV and ANIV are sufficiently different (62% and 68% homology for amino acid sequences, respectively).
Asunto(s)
Charadriiformes/virología , Ixodes/virología , Orbivirus/genética , Secuencia de Aminoácidos , Animales , Charadriiformes/genética , Ixodes/genética , Orbivirus/patogenicidad , Filogenia , ARN Viral/genéticaRESUMEN
The Tyulek virus (TLKV) was isolated from the ticks Argas vulgaris Filippova, 1961 (Argasidae), collected from the burrow biotopes in multispecies birds colony in the Aksu river floodplain near Tyulek village (northern part of Chu Valley, Kyrgyzstan). Recently, the TLKV was assigned to the Quaranfil group (including the Quaranfil virus (QRFV), Johnston Atoll virus (JAV), Lake Chad virus) that is a novel genus of the Quaranjavirus in the Orthomyxoviridae family. In his work, the complete genome (ID GenBank KJ438647-8) sequence of the TLKV was determined using next-generation sequencing (Illumina platform). Comparison of deduced amino acid sequences shows closed relationship of the TLKV with QRFV and JAV (86% and 84% identity for PB1 and about 70% for PB2 and PA, respectively). The identity level of the TLKV and QRFV in outer glycoprotein GP is 72% and 80% for nucleotide and amino acid sequences, respectively. The phylogenetic analysis showed that the TLKV belongs to the genus of the Quaranjavirus in the family Orthomyxoviridae.
Asunto(s)
Aves/virología , Orthomyxoviridae/genética , Filogenia , Garrapatas/virología , Animales , Clasificación , Kirguistán , Orthomyxoviridae/clasificación , Orthomyxoviridae/aislamiento & purificación , PolímerosRESUMEN
The prototype strain LEIV-K306 of the Batken virus (BKNV) was isolated from the Ixodidae ticks Hyalomma marginatum Koch, 1844 collected from sheep near town Batken (Kirgizstan) in the April 1970. Later, the BKNV was isolated in Kirgizstan from the mixed pool of the Aedes caspius Pallas, 1771 and Culex hortensis Ficalbi, 1889 mosquitoes. From the very beginning, the BKNV was discussed to be very close to the Dhori virus (DHOV) (Orthomyxoviridae, Thogotovirus) isolated from the Ixodidae ticks Hyalomma dromedarii Koch, 1844 in India. In this work, virtually complete genome sequence (MiSeq, Illumina) of the BKNV was determined (ID GenBank KJ396672-4). Structural and non-structural proteins of the BKNV have a high level of homology with DHOV - 98% (PB1) and 96% (PB2, PA, NP, M). Homology of HA protein between the BKNV and DHOV is 90%, which accounts for antigenic difference between these close relative viruses. Since the differences in the other structural and non-structural proteins are about 96-98%, the BKNV could be suggested as the topotypic DHOV strain for Central Asia, Transcaucasia, and Northern Caspian region. The evolution divergence of the BKNV and DHOV for HA could be explained by local ecologic peculiarities of the BKNV areal.
Asunto(s)
Genoma Viral , Orthomyxoviridae/genética , Análisis de Secuencia de ADN , Aedes/virología , Animales , Biota , Aves/virología , Culex/virología , Biblioteca de Genes , Ixodes/virología , Orthomyxoviridae/clasificación , Orthomyxoviridae/aislamiento & purificaciónRESUMEN
The full-length genome of the unclassified Geran virus (GERV, strain LEIV-10899Az) isolated from the ticks (Ornithodoros verrucosus Olenev, Zasukhin and Fenyuk, 1934 (Argasidae, Ornithodorinae)) collected in the burrow of the red-tailed gerbils (Meriones (Cricedidae) erythrourus Grey, 1842) near the Geran station (Azerbaijan) was sequenced using the next-generation approach (GenBank ID: KF801649). It was shown that the GERV is a new representative of the Nairovirus genus (family Bunyaviridae). The comparative analysis of the full-length genome sequences of the GERV with other nairoviruses showed that the highest level of homology (55.6% for N protein (S-segment) of 54.2% for the polyprotein Gn/Gc (M-segment) and 74.8% for the RNA-dependent RNA polymerase (L-segment)) GERV had with the Chim virus (CHIMV) that is also associated with the shelters biocenoses (rodent burrows) in Central Asia and was previously assigned to the Qalyub virus group (QYBV). Comparing the GERV with the QYBV sequences (partial sequence 413 n.o. of RdRp gene) revealed a high level of homology: 74.3 and 97.4% for the nucleotide and amino acid sequences, respectively. The data obtained in this work provided an opportunity to classify the GERV to the QYBV group; the Nairovirus genus, to the family Bunyaviridae.
Asunto(s)
Infecciones por Bunyaviridae/veterinaria , Genoma Viral , Gerbillinae/virología , Nairovirus/genética , Ornithodoros/virología , Filogenia , Proteínas Virales/genética , Secuencia de Aminoácidos , Animales , Azerbaiyán/epidemiología , Secuencia de Bases , Infecciones por Bunyaviridae/epidemiología , Infecciones por Bunyaviridae/transmisión , Infecciones por Bunyaviridae/virología , Vectores de Enfermedades , Secuenciación de Nucleótidos de Alto Rendimiento , Datos de Secuencia Molecular , Nairovirus/clasificación , Nairovirus/aislamiento & purificación , Homología de Secuencia de AminoácidoRESUMEN
The complete genome of Uzun-Agach virus (UZAV), isolated from the liver of Myotis blythii oxygnathus (Monticelli, 1885 (Chiroptera; Vespertilionidae)) bats in Alma-Ata district (Kazakhstan) in 1977 have been sequenced. Based on full-length genome comparison it is shown that UZAV is a new member of the Nairovirus genus (family Bunyaviridae). L-segment and M-segments of UZAV have 69,3% and 64,1% identity with Issyk-Kul virus (ISKV) that also was isolated from bats. S-segment of UZAV have 99,6% identity with the same of ISKV. This allow us to claim that UZAV is a reassortant virus that have S-segment derived from ISKV, and L- and M-segments from another virus that is phylogenetically related to ISKV, but divergent from it. The obtained data that the reassortment between ISKV and UZAV exists in nature suggest that they cocirculated in one ecological niche (bats of the Vespertilionidae family) and the areal of UZAV may coincide with the areal of ISKV.
Asunto(s)
Infecciones por Bunyaviridae/veterinaria , Quirópteros/virología , Genoma Viral , Nairovirus/genética , Filogenia , Virus Reordenados/genética , Proteínas Virales/genética , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Infecciones por Bunyaviridae/epidemiología , Infecciones por Bunyaviridae/transmisión , Infecciones por Bunyaviridae/virología , Secuenciación de Nucleótidos de Alto Rendimiento , Kazajstán/epidemiología , Datos de Secuencia Molecular , Nairovirus/clasificación , Nairovirus/aislamiento & purificación , Virus Reordenados/clasificación , Virus Reordenados/aislamiento & purificación , Homología de Secuencia de Ácido NucleicoRESUMEN
Complete genome sequencing of the Kyzylagach virus (KYZV) LEIV-65A strain isolated from the Culex modestus Ficalbi, 1889 (Culicinae), which was collected in the colony of the Ardeidae Leach, 1820 birds on the coast of Caspian sea, Kyzyl-Agach bay, in the southern part of Azerbaijan, was carried out. KYZV has high homology (about 99%) with the Chinese XJ-160 strain of the Sindbis virus (SINV) isolated from Anopheles sp. in Xinjiang Uyghur autonomic region (north-eastern China). Homologies of KYZV and XJ-160 with European SINV strains are 82% and 93% for the nucleotide and amino acid sequences, respectively (GenBank ID: KF981618). The difference between the nucleotide sequences of KYZV and Australian SINV/SW6562 strain is 19%; amino acid sequences, 12%. Since XJ-160 strain is extremely similar to KYZV, the first could be considered as the KYZV strain. The geography of the KYZV and XJ-160 isolation points and their genetic distance from the European viruses allow the KYZV to be suggested as a SINV (genotype IV) topotypic variant typical of Transcaucasia and Central Asia.
Asunto(s)
Infecciones por Alphavirus/veterinaria , Aves/virología , Culex/virología , Genoma Viral , Filogenia , Virus Sindbis/genética , Proteínas Virales/genética , Infecciones por Alphavirus/epidemiología , Infecciones por Alphavirus/transmisión , Infecciones por Alphavirus/virología , Secuencia de Aminoácidos , Animales , Azerbaiyán/epidemiología , Secuencia de Bases , Vectores de Enfermedades , Secuenciación de Nucleótidos de Alto Rendimiento , Datos de Secuencia Molecular , Homología de Secuencia de Aminoácido , Homología de Secuencia de Ácido Nucleico , Virus Sindbis/clasificación , Virus Sindbis/aislamiento & purificaciónRESUMEN
Almost complete nucleotide sequences for the S, M, and L segments were obtained for three strains of the Batai virus (Bunyamwera serogroup, genus Orthobunyavirus, Bunyaviridae family). Based on the results of the phylogenetic analysis conducted forthe three genomic segments LEIV Ast507 and LEIV-Ast528 strains were grouped with other European BATV isolates and were found to be almost identical to the strain 42 isolated from Volgograd Region, Russia, 2003. Surprisingly, LEIV-13395 strain isolated from the Aedes sp. mosquitos in Magadan Oblast, 1987, turned out to be a novel genotype inside Bunyamwera serogroup. The highest nucleotide identity levels of LEIV-13395 genomicsegments (86.9%, 80.8%, 79.7% for S, M and L segments respectively) were observed with corresponding segments of the Batai virus.
Asunto(s)
Aedes/virología , Virus Bunyamwera/genética , Infecciones por Bunyaviridae/veterinaria , Genoma Viral , Insectos Vectores/virología , Filogenia , Animales , Secuencia de Bases , Aves/virología , Encéfalo/virología , Virus Bunyamwera/clasificación , Virus Bunyamwera/aislamiento & purificación , Virus Bunyamwera/metabolismo , Infecciones por Bunyaviridae/epidemiología , Infecciones por Bunyaviridae/virología , Chlorocebus aethiops/virología , Genotipo , Glicosilación , Ratones , Datos de Secuencia Molecular , Federación de Rusia/epidemiología , Homología de Secuencia de Ácido Nucleico , Células Vero , Proteínas Virales/genética , Proteínas Virales/metabolismoRESUMEN
Complete genome sequence of the Burana virus (BURV) was determined using the next-generation sequencing approach (ID GenBank KF801651). The prototype strain of BURV LEIV-Krg760 was originally isolated from the ticks Haemaphysalis punctata Canestrini et Fanzago, 1877 (Ixodidae, Haemaphysalinae), collected from cows in Tokmak wildlife sanctuary, eastern part of the Chu valley (43 degrees 10' N, 74 degrees 40' E) near Burana village, Kirgizia, in April 1971. Molecular genetics and phylogenetic analyses showed that the BURV belonged to the Nairovirus genus, Bunyaviridae and is related to Tamdy virus (TAMV) that is also associated with the ixodidae ticks of pasture biocenosis in Central Asia. Previous studies showed that TAMV is the prototypic virus of new phylogenetic Tamdy group in the Nairovirus genus. Thus, BURV was classified as a new virus of the Tamdy group, Nairovirus, Bunyaviridae.
Asunto(s)
Bunyaviridae/genética , Genoma Viral/genética , Secuenciación de Nucleótidos de Alto Rendimiento , Ixodidae/genética , Animales , Bovinos , Kirguistán , Datos de Secuencia Molecular , Filogenia , Garrapatas/virologíaRESUMEN
The Syr-Darya valley fever virus (SDVFV) was originally isolated from the blood of the patient with fever in the Kyzylorda province, Kazakhstan, in July 1973 and was classified to the Cardiovirus genus (fam. Picornaviridae). Later, SDVFV was isolated from the ticks Hyalomma as. asiaticum Schulze et Schlottke, 1929 (Hyalomminae) (1 strain) and Dermacentor daghestanicus Olenev, 1929 (Rhipicephalinae) (7 strains), collected in the floodplains of the Syr-Darya river and the Ili river. In this paper, complet genome of the SDVFV (strain LEIV-Tur2833) was sequenced using the next-generation sequencing approach (GenBank ID: KJ191558). It was demonstrated that, phylogenetically, the SDVFV is closely related closest to the Theiler's murine encephalomyelitis virus (TMEV) and Vilyuisk human encephalomyelitis virus (VMEV). The similarity of the SDVFV with VHEV and TMEV based on P1 region of the polyprotein-precursor (structural proteins VP1-VP4), reaches 75% and 91% for nucleotide sequences and 80% and 93% for putative amino acid sequences, respectively. For nonstructural proteins regions P2 (2A-2C) and P3 (3A-3D) similarity of SDVFV with TMEV and VHEV is 96%-98%.
Asunto(s)
Genoma Viral , Filogenia , Infecciones por Picornaviridae/virología , Picornaviridae/genética , Secuencia de Aminoácidos , Animales , Argasidae/virología , Aves/virología , Humanos , Ixodidae/virología , Kazajstán , Metagenoma , Datos de Secuencia Molecular , Picornaviridae/patogenicidad , Infecciones por Picornaviridae/genética , TurkmenistánRESUMEN
Near full-genome sequence of the Wad Medani Virus (WMV) (strain LEIV-8066Tur) (Orbivirus, Reoviridae) isolated from the ticks Hyalomma asiaticum Schulze et Schlottke, 1929, collected from sheep in Baharly district in Turkmenistan, was determined using next generation sequencing approach. The similarity of the RNA-dependent RNA-polymerase (Pol, VP1) amino acid sequence between WMV and the Kemerovo group orbiviruses (KEMV), as well as of the Baku virus (BAKV), was 64%. The similarity of the conserved structural protein VP3 (T2) of WMV with mosquito-borne and tick-borne orbiviruses reaches 46% and 67%, respectively. For the surface proteins VP2, VP5, and VP7 (T13), which have major antigenic determinants of orbiviruses, the similarity of WMV with tickborne orbiviruses (KEMV and BAKV) is 26-30%, 45% and, 57%, respectively (ID GenBank: KJ425426-35).