Simulation-Assisted DNA Nanodevice Serve as a General Optical Platform for Multiplexed Analysis of Micrornas.

Small frame nucleic acids (FNAs) serve as excellent carrier materials for various functional nucleic acid molecules, showcasing extensive potential applications in biomedicine development. The carrier module and function module combination is crucial for probe design, where an improper combination can significantly impede the functionality of sensing platforms. This study explores the effect of various combinations on the sensing performance of nanodevices through simulations and experimental approaches. Variances in response velocities, sensitivities, and cell uptake efficiencies across different structures are observed. Factors such as the number of functional molecules loaded, loading positions, and intermodular distances affect the rigidity and stability of the nanostructure. The findings reveal that the structures with full loads and moderate distances between modules have the lowest potential energy. Based on these insights, a multisignal detection platform that offers optimal sensitivity and response speed is developed. This research offers valuable insights for designing FNAs-based probes and presents a streamlined method for the conceptualization and optimization of DNA nanodevices.

Acute Mesenteric Ischemia in Patients with COVID-19: Review of the literature.

The coronavirus disease 2019 (COVID-19) pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has resulted in a global health emergency. In addition to common respiratory symptoms, some patients with COVID-19 infections may experience a range of extra-pulmonary manifestations, such as digestive system involvement. Patients with COVID-19 have been reported to suffer from acute mesenteric ischemia (AMI) that is associated with disease-related severity and mortality. However, in the context of COVID-19, the exact cause of AMI has yet to be clearly defined. This review provides a comprehensive overview of the available data and elucidates the possible underlying mechanisms linking COVID-19 to AMI, in addition to highlighting therapeutic approaches for clinicians. Finally, given the severe global impact of COVID-19, we emphasize the importance of coordinated vaccination programs.

A lateral flow dipstick combined with reverse transcription recombinase polymerase amplification for rapid and visual detection of the BVDV and BPIV3.

Bovine respiratory disease complex (BRDC) is a serious disease affecting feedlot cattle in China and likely other places worldwide. Bovine viral diarrhea virus (BVDV) and bovine parainfluenza virus type 3 (BPIV3) are principally responsible for causing BRDC, and are a major strain to the industrial economy. Eradication of these viruses/disease requires swift viral identification and treatment. Hence, this study established a fast and easy procedure of BVDV and BPIV3 identification that employs reverse transcription recombinase polymerase amplification (RT-RPA) and lateral flow dipstick (LFD), and uses primers and lateral flow (LF) probe targeting the 5'-UTR gene of BVDV and phosphoprotein P gene of BPIV3, respectively. Our assay was able to successfully amplify BVDV and BPIV3 RNA within 25 min at 35 °C using RT-RPA, with products visible on the LFD within 5 min at room temperature (RT). The lowest detection limits were 50 RNA molecules for BVDV and 34 RNA molecules for BPIV3 per reaction. We also demonstrated that the established dual RT-RPA LFD assay was precise and targeted, harboring excellent potential to become an onsite molecular diagnostic tool in the detection of BVDV and BPIV3. This method can detect BVDV (Pestivirus A, B) and BPIV3, and exhibit no cross-reaction with other viruses like the classical swine fever virus (CSFV) and infectious bovine rhinotracheitis virus (IBRV). The assay performance was further assessed with clinical samples, and demonstrated good performance in comparison to real-time RT-PCR (RT-qPCR). Moreover, the RT-RPA LFD assay was comparitively rapid and required minimal training.

[Polycyclic Aromatic Hydrocarbons in Surface Soil of China (2000-2020): Temporal and Spatial Distribution, Influencing Factors].

With the rapid development of China's economy and society, the polycyclic aromatic hydrocarbon (PAH) pollution of surface soil has attracted increasing attention. Based on a systematic review, this study identified 166 relevant papers (published from 2000 to 2020) dealing with the contamination of 16 PAHs in the surface soil of China and summarized the pollution level, temporal, and spatial distribution influencing factors of PAHs with statistics, spatial interpolation analysis, and source analysis methods. The results showed that the surface soil of China has been polluted by human-caused PAHs, with a median concentration of 675.70 µg·kg-1. Although the overall condition is good, some sampling points have been seriously polluted. Among the monomers of PAHs, the concentrations of fluoranthene (Fla) and pyrene (Pyr) are high, while acenaphthylene (Acy) and acenaphthene (Ace) are relatively low. During the survey period, the concentration data of surface soil PAHs are generally within the moderate pollution levels of 313.10-1070.45 µg·kg-1, while the annual changes of PAHs do not show obvious fluctuations and are less affected by oil production and consumption. Statistics and spatial interpolation results show that PAH pollution in the surface soil of China has regional characteristics, where the concentration decreases in order from northwest, north, east, northeast, southwest, and south-central China. The pollution level in most provinces is "contaminated" or "weakly contaminated." From the source analysis results, PAH pollution in surface soils in most areas of China comes from the high-temperature combustion of fossil fuels such as petroleum, biomass, and coal. Heilongjiang and some northwestern regions (e.g., Xinjiang and Tibet) were mainly represented by oil source pollution. Such results could provide a reference for soil environmental management and PAH pollution control in China.

Targeting of phospho-eIF4E by homoharringtonine eradicates a distinct subset of human acute myeloid leukemia.

More than half of the patients with acute myeloid leukemia (AML) fail to achieve long-term disease-free survival with current therapies and novel therapeutic strategies are urgently needed. The effects of homoharringtonine (HHT) on the growth of AML cell lines and primary leukemia cells were examined using MTT, colony formation assay. The effects of HHT on both eukaryotic translation initiation factor 4E (eIF4E) and phospho-eIF4E(p-eIF4E) were examined through western blot and immunofluorescence staining. HHT selectively reduced levels of p-eIF4E and its downstream oncoprotein Mcl-1, and potently inhibited in vitro and in vivo the growth of a distinct subset of AML cells and primary leukemia cells expressing high level of p-eIF4E through apoptosis. Our findings suggest that HHT might be a first-in-class p-eIF4E-targeted drug and offer a novel therapeutic option for AML patients expressing high level of p-eIF4E.

Katanin P80 expression correlates with lymph node metastasis and worse overall survival in patients with breast cancer.

OBJECTIVE: The aim of this study was to investigate the correlation of katanin P80 expression with clinicopathological features and overall survival (OS) in surgical breast cancer (BC) patients. METHODS: Four hundred and fourteen BC patients underwent surgery were analyzed in this retrospective cohort study. Katanin P80 expression was examined by immunofluorescence assay. The median follow-up duration was 118.0 months (quantiles: 99.0-140.5 months), the last follow-up date was Jul 1st 2017. RESULTS: Eighty-five patients (20.5%) with katanin P80 positive expression and 329 patients (79.5%) with katanin P80 negative expression were observed in this research. Katanin P80 positive expression was correlated with higher N stage (p< 0.001) and TNM stage (p< 0.001). K-M curve and log-rank test revealed that katanin P80 positive patients presented with shorter OS compared with katanin P80 negative patients (p< 0.001). Multivariate Cox's regression analysis disclosed that katanin P80 positive expression (p< 0.001) and histologic grade (p< 0.001) could independently predict unfavorable OS. Furthermore, subgroups analysis was performed, which illuminated that katanin P80 positive expression was correlated with shorter OS in all subgroups divided by molecular subtyping and TNM stage (all p< 0.05) except in TNM stage I subgroup (p= 0.573). CONCLUSION: Katanin P80 expression positively correlated with lymph node metastasis and could abe a novel biomarker for prognosis in BC patients.

[Prognostic Value of Morphology and Hans Classification in Diffuse Large B Cell Lymphoma].

OBJECTIVE: To investigate the prognostic value of morphology and Hans classification in diffuse large B cell lymphoma（DLBCL）. METHODS: Clinical data of 249 patients diagnosed with DLBCL in our hospital and Hangzhou Xixi hospital during Jan 2006 to Dec 2016 were analyzed retrospectively. These patients were classified into 3 groups: immunoblastic variant（IB） group, centroblastic variant（CB） group and others group according to the cell morphology. And DLBCL was also divided into GCB（germinal center B-cell-like）or non-GCB（non-germinal center B-cell-like） group by analyzing the expression of CD10, BCL6 and MUM1 (GCB: CD10 +,BCL6+-,MUM1+-/CD10-,BCL6+,MUM1-；non-GCB：CD10-,BCL6-,MUM1+-/CD10-,BCL6+,MUM1+). RESULTS: The univariate analysis displayed that the age，LDH level，IPI，IB，non-GCB，B-symptoms and rituximab all could influence the OS and EFS, the CR rate of CB subtype patients was significantly higher than that of the patients with IB subtype （68.3% vs 38.9%)(P=0.02）. IB subtype was the in dependent prognostic factor for both EFS and OS in the whole study. In multivariate analysis, IPI and IB were the independent prognostic factors for OS and EFS. IB subtype was also an independent prognostic factor in EFS and OS with or without rituximab. The expression of BCL2 and BCL6 was related with prognosis in R-CHOP, but not in CHOP treated patients. Other markers (CD5, CD10, IRF4/MUM1, HLA-DR and Ki-67 proliferation index) were not of the significant prognostic value for DLBCL. When accepted rituximab, the GCB and non-GCB were not different significantly for prognosis. However, the non-GCB group showed a poor prognosis without using rituximab （EFS P=0.020；OS P=0.020）. Multivariate Cox models showed that OS and EFS were not significantly different between GCB and non-GCB group, however, the IB subtype had a very significantly poor prognosis in OS and EFS (P=0.001, P=0.002). When the analysis was restricted to DLBCL with CB morphology only, no prognostic value was observed in Hans classification. CONCLUSION: The subtype of immunoblast is a major risk factor in patients treated with CHOP or R-CHOP. There is a significant association between the Hans classification and the morphologic subclassification. Results of this study have supplemented the data for the prognostic factor of DLBCL and demonstrated that the cytomorphologic diagnosis can be reproducible.

Several [Gd-M] Heterometal-Organic Frameworks with [Gdn] as Nodes: Tunable Structures and Magnetocaloric Effect.

Tunable structures and magnetocaloric effect (MCE) of seven MOFs with [Gdn] as nodes are explored. The [Gdn] node is realized from mononuclear [Gd] to binuclear paddle-wheel [Gd2], tetranuclear tetrahedral [Gd4], and pentanuclear trigonal bipyramidal [Gd5]. Meanwhile, the magnetic entropy changes from 19.4 to 46.0 J·kg-1·K-1. The results reveal that the effect of magnetic density on MCE plays a dominant role for Gd3+-based compounds, and high spin ground state of Mn2+ (S = 5/2) is more favorable to achieve high MCE than that with Zn2+, Co2+, and Ni2+ (Co2+, S = 3/2; Ni2+, S = 1). To our knowledge, it is the first report that MCE is controlled by various clusters as nodes in MOFs.

[Effect of long noncoding RNA SPRY4-IT1 on proliferation and metastasis of medulloblastoma].

OBJECTIVE: To investigate the effects of long non-coding RNA SPRY4-IT1 (LncRNA) on proliferation and metastasis of medul-loblastoma cells. METHODS: SPRY4-IT1siRNA and control fluorescence siRNA were transfected into medulloblastoma cell line Daoy with Lipo-fe ctamine 2000 and were divided into control group and si-SPRY4-IT1 group. Relative expression of SPRY4-IT1 mRNA were detected by real-time quantitative PCR. The change of cell proliferation were examined using CCK-8 kit and clone forming experiment. The change of cell inva-sion and metastasis were examined by matrigel invasion assay and cell metastasis experiment respectively, The expression of matrix metallopro-teinase MMP-2 and MMP-9 were detected by Western blot. RESULTS: In si-SPRY4-IT1 group,the SPRY4-IT1 mRNA expression level, cell pro-liferation in vitro,cell invasion and migration ability, MMP-2 protein expression were significantly lower than those in the control group. CONCLUSIONS: Interference SPRY4-IT1 expression has prominent inhibitory effect on the cell proliferation、invasion and metastasis of medulloblastoma cell line Daoy.

[Nitrogen flow characteristic and use efficiency in mixed crop-livestock system in North China Plain: Hebei Jinlong Circular Agriculture Farm as an example]. / åŽåŒ—å¹³åŽŸç§å »ä¸€ä½“è§„æ¨¡åŒ–å†œåœºæ°®ç´ æµåŠ¨ç‰¹å¾åŠåˆ©ç”¨æ•ˆçŽ‡­­ä»¥æ²³åŒ—津龙循环农业园区为例.

Scale and intensive development of mixed crop-livestock agriculture is inevitable in North China Plain (NCP), and nitrogen (N) is one of the key elements that linked the crop and livestock production. Here, we used Hebei Jinlong Circular Agriculture Farm as an example, to analyze the N flow and use efficiency, by using data from literature, on-farm survey and modeling. Further, several on-farm N management improvement practices were proposed and evaluated through scenario analysis, to supply technical support and scientific basis not only for achieving higher N use efficiency and productivity at the farm level, but also providing a new model of circular agriculture in NCP. Our results indicated that manure and purchased fertilizer were the main N input in crop production, which amounted to 674.6 kg N·hm-2·a-1 and accounted for 88.3% of the total N input. Of all the N input in crop production system, only 41.5% ended up in the crop products and around 190.7 kg N·hm-2·a-1 surplus. The excess input of synthetic fertilizer was the main reason for low N use efficiency and high N surplus for crop system. Purchased feed was the main N input pathway in livestock production system, and accounted for 83.2% of the total N input. The annual N excretion rate was 776.6 t N, and around 36.3% of the excreted N was recycled to the crop system. The N use efficiency was 19.7% for livestock production system. The N use efficiency was 40.7% at the whole farm level. The scenarios analysis showed that reducing purchased fertilizer N input by 50% (scenario 1) and increasing the total maize production via adjustment of cropping structure (scenario 2) would increase the N use efficiency in cropping system by 18.7% and 9.8%, respectively. The whole farm use efficiency could be increased by 19.1% through optimizing the feed compositions and regimes (scenario 3). Therefore, reducing purchased fertilizer N input, adjustment of cropping structure and optimizing the feed compositions could increase the N production capacity and achieve an environmental friendly mixed crop-livestock production system simultaneously in NCP.

[Effect of matrine on cell apoptosis and proliferation and the apoptosis related proteins of human medulloblastoma D341 cells in vitro].

OBJECTIVE: To investigate the apoptosis and proliferation effect of matrine on human medulloblastoma cell line D341 in vitro and the effect of the expression of the related caspase 3 and caspase 9 proteins. METHODS: The D341 cells were cultivated successfully in vitro. Then the cells were divided into 5 groups according to the concentration of matrine (0.5 mg/mI group, 1.0 mg/ml group, 1.5 mg/ml group, 2.0 mg/ml group and the control group was 0 mg/ml). All the experiments were repeated three times. The cell morphologic and structure change was observed with the optical microscope and the transmission electron microscope. The proliferation of D341 cell was analyzed using Cell Counting Kit-8 assay. Apoptosis was detected by Annexin V-FITC/PI double staining. The expression of Caspase3 and Caspase9 was detected by Western blot. RESULTS: With the effect of matrine, the proliferation inhibition rate gradually increased with drug concentrations increasing, and there was a significant difference (P < 0.01). The inhibitory effect of matrine on cell proliferation was different with the different treatment time, there was a significant difference between the 24 h to 72 h groups (P < 0.01). The apoptotic rate increased with matrine concentrations increasing. There were significant differences between the group of 0.5 mg/mI or 1.0 mg/mI to the group of 1.5 mg/mI or 2.0 mg/mI (P < 0.05). The apoptotic rate increased with the prolonged treatment time. There were significant differences between the group of 24 h or 48 h to the group of 72 h ( P < 0.05). With the increase of matrine concentration, the expression of Caspase 3 and Caspase 9 increased (P < 0.01). CONCLUSION: Matrine induces the apoptosis, and inhibits the proliferation of human medulloblastoma D341 cells in vitro by up-regulation of the expression level of Caspase3, Caspase9.

[The relationship between hypermethylation of Syk gene promoter and medulloblastoma cell invasion and metastasis].

OBJECTIVE: To investigate the effect of demethylation of Syk gene promoter by the methylation transferase inhibitor 5-aza-CdR on the invasion and metastasis of medulloblastoma cell line Daoy. METHODS: Medulloblastoma cell line Daoy was treated with 5-aza-CdR in vitro. Methylation-specific PCR, real time-PCR and Western blot were used to detect Syk gene promoter methylation status, Syk mRNA and protein expression respectively. Transwell was employed to study the invasion and metastasis of medulloblastoma cell line Daoyby counting the cells that had invaded through Matrigel and migrated to the undersurface of the membrane before and after treatment of 5-aza-CdR. RESULTS: In comparison to control group, Syk gene promoter of 5-aza-CdR-treated groups was demethylated and expression of Syk mRNA and protein was significantly up-regulated by 3.40±0.24 folds (P<0.01) and 3.23±0.19 folds (P<0.01) respectively. The invasiveness and metastasis of medulloblastoma cell line Daoy was decreased(P<0.05). CONCLUSIONS: Hypermethylation of Syk gene promoter is responsible for the down-regulation of Syk gene expression in medulloblastoma cell line Daoy, which may be one of the mechanisms that enhanced cell invasion and metastasis. While 5-aza-CdR can reverse the hypermethylation of Syk gene promoter and restore Syk gene expression and thus suppresses invasiveness and metastasis of tumor cells.

Ultrastrong Alkali-Resisting Lanthanide-Zeolites Assembled by [Ln60] Nanocages.

Zeolites, as one of the most important porous materials, are most widely utilized in sorbents, catalysis, and ion-exchange fields. However, the multi-functional lanthanide-zeolites constructed exclusively by lanthanide ions and oxygen linkers are to our knowledge unknown hitherto. Herein, we, for the first time, report the unique structure and multifunctions of lanthanide zeolites (1·Gd, 1·Tb, 1·Dy), featuring 60 nuclear [Ln60] nanocages as building blocks and ultrastrong alkali-resisting. These compounds possess extremely high stability and still retain single crystallinity after treatment in boiling water, 0.1 M HCl, and 20 M NaOH aqueous solutions. Magnetic studies revealed 1·Gd has large magnetocaloric effect with -ΔSm(max) = 66.5 J kg(-1) K(-1), falling among the largest values known to date. Importantly, these lanthanide-zeolites themselves can efficiently catalyze the cycloaddition of CO2 with epoxides under mild conditions. Our finding extends the conventional zeolites to lanthanide counterparts, opening a new space for seeking novel and/or multifunctional zeolites.

Heterometal-organic frameworks as highly sensitive and highly selective luminescent probes to detect I⁻ ions in aqueous solutions.

Two cationic heterometal-organic frameworks (Eu-Zn (1·NO3⁻) and Tb-Zn (2·NO3⁻)) with NO3(-) counter-anions in the channels are structurally and luminously characterized. Both of them can serve as highly sensitive and highly selective luminescent probes for detecting I(-) ions in aqueous solutions. In particular, 2·NO3⁻ can selectively and reversibly detect I(-) with a fast response time of just 10 s and an extremely low detection limit of 0.001 ppm. Mechanism studies reveal that I(-) is quickly oxidized to form I3⁻with the help of 1·NO3⁻ or 2·NO3⁻, leading to luminescence quenching. This represents the first report of MOF-based luminescent probes for the detection of I(-) in aqueous solutions.

New strategy to construct single-ion magnets: a unique Dy@Zn6 cluster exhibiting slow magnetic relaxation.

Two unique heptanuclear clusters Ln@Zn6 (Ln = Dy (1), Er (2)) were structurally and magnetically characterized. Each Dy(3+)/Er(3+) is located in a nona-coordinate D(3h) coordination environment, and is encapsulated in a diamagnetic Zn6 cage. Compound 1 exhibits single-ion magnetic behavior, and is the first example of a single-ion magnet (SIM) constructed through embedding one magnetic anisotropic metal ion into a diamagnetic cage.

Unique (3,12)-connected coordination polymers displaying high stability, large magnetocaloric effect and slow magnetic relaxation.

Two unique 3D coordination polymers based on octahedral [Ln6] (Ln = Gd (), Dy ()) clusters as nodes were synthesized, exhibiting a novel (3,12)-connected topology with the symbol of (4(20)·6(26)·8(20))(4(3))4. The magnetic studies reveal that shows a -ΔSm = 46.6 J kg(-1) K(-1), falling among the largest values known to date, and displays slow magnetic relaxation behavior. Both and have high thermal and solvent stabilities.

Structures, luminescence, and slow magnetic relaxation of eight 3D lanthanide-organic frameworks.

Eight three-dimensional lanthanide-organic frameworks: [Ln(BPDC)1.5(DMF)(H2O)2]·2H2O (Ln = Eu (1), Gd (2), Tb (3), Dy (4); BPDC = 4,4'-dicarboxylate-2,2'-dipyridine anion), [Ln(BPDC)(DMF)2(NO3)] (Ln = Eu (5), Gd (6), Tb (7), Dy (8)) were fabricated and structurally characterized. Compounds 1-4 are isostructural, belonging to the triclinic system with space group P1¯, while compounds 6-8 belong to the monoclinic system with space group C2/c. Structural differences between two types of compounds may be caused by different reaction conditions. Magnetic properties of 2-4 and 6-8 have been investigated and only compounds 4 and 8 display significant frequency-dependence, albeit without reaching the characteristic maxima above 2 K, implying slow magnetic relaxation behaviors in 4 and 8. After the application of a DC field, good peak shapes of AC signal were obtained and got the energy barrier for 4, ΔE/kB = 79.80 K and the pre-exponential factor τ0 = 1.28 × 10(-10) s, for 8, ΔE/kB = 38.15 K and τ0 = 2.47 × 10(-9) s. Geometrical differences in the crystal fields of Dy(3+) in 4 and 8 seem to be responsible for the large divergence of their magnetic behaviors. Luminescence analyses were performed on coordination polymers containing Eu(3+), Tb(3+), and Dy(3+), which exhibit the characteristic transitions of corresponding lanthanide ions, and give the lifetime (τ0) of 1, 3, 4, 5, 7 and 8 are 0.56 ms, 0.89 ms, 8.48 µs, 0.60 ms, 0.75 ms and 36.35 µs, respectively.

Anion-induced changes of structure interpenetration and magnetic properties in 3D Dy-Cu metal-organic frameworks.

Two novel Dy-Cu MOFs were structurally and magnetically characterized. The results reveal that it is the first example of anion-induced change of structure interpenetration and magnetic properties in 3d-4f MOFs.

Structural diversity and magnetic properties of five copper-organic frameworks containing one-, two-, and three-types of organic ligands.

Five novel compounds {[Cu(CMA)]·0.8H(2)O}(n) (1), {[Cu(CMA)(bpy)(0.5)]·0.5DMF}(n) (2), {[Cu(CMA)(bpy)(H(2)O)]·0.5H(2)O}(n) (3), {[Cu(2)(CMA)(DPA)(bpy)(2)(H(2)O)]ClO(4)}(n) (4) and {[Cu(3)(CMA)(DPA)(bpy)(4)(OH)](ClO(4))(2)·3H(2)O)}(n) (5) (H(2)CMA = 3-(carboxymethoxy)-5-methylbenzoic acid, HDPA = 2-(3,5-dimethylphenoxy)acetic acid, bpy = 4,4'-bipyridine) were synthesized and characterized by single crystal X-ray diffraction. Interestingly, the kinds of ligands included in these compounds are different: 1 contains single-ligand CMA(2-); 2 and 3 comprise two kinds of ligands (CMA(2-) and bpy); 4 and 5 cover three kinds of ligands (CMA(2-), DPA(-) and bpy). Structural analyses reveal that 1-3, and 5 are three-dimensional (3D) frameworks, while 4 is a 2D layer. Noteworthily, 1 possesses two types of channels: six-star and hexagonal ones. 3 displays a three-fold interpenetrating 3D framework. The structural diversity of the compounds may have originated from different anions, solvents and temperatures. Variable-temperature magnetic susceptibility measurements were carried out on compounds 1, 2 and 4, and the magnetic properties are dramatically different due to the structural diversities. The magnetic data of 1 shows an antiferromagnetic interaction with J = -54.84 cm(-1) estimated from the Bonner-Fisher model. The magnetic data of 2 was least-square fitted to the Blenaey-Bowers equation with J = -131.01 cm(-1), indicating the existence of a strong antiferromagnetic interaction between two adjacent Cu(2+). The magnetic data of linear tetranuclear structure 4 was best fitted to the expression derived from the Hamiltonian H = -2(2J(1)S(1)S(2) + J(2)S(1)S(1*)). The best fitting parameters are J(1) = -17.48 cm(-1) and J(2) = -65.26 cm(-1), which also indicate an antiferromagnetic interaction. Simultaneously, the magneto-structural relationship was discussed.

3 D†MOFs containing trigonal bipyramidal Ln5 †clusters as nodes: large magnetocaloric effect and slow magnetic relaxation behavior.

Two 3D heterometal-organic frameworks based on infrequent trigonal bipyramidal Ln(5)  clusters as nodes were structurally and magnetically characterized (Ln=Gd (1), Dy (2)). The results indicate large MCE of up to 30.7 J kg(-1)  K(-1) in 1 and slow magnetic relaxation behavior in 2. Expectedly, constructing 3D MOFs based on multinuclear clusters as nodes may will be a new strategy for achieving large -ΔS(m). Additionally, compound 1 exhibits high thermal and solvent stabilities, providing a favorable foundation for realistic applications.