RESUMEN
To improve the problem that self-synchronization and control synchronization of vibration systems are respectively restricted by their inherent characteristics and product cost, by combining them a multi-phase composite synchronization scheme is proposed for space far-resonant vibration system actuated by three vibrators. Based on the established mathematical model and sliding mode control (SMC) algorithm, controller of one vibrator is designed to implement the multi-phase control synchronization. Then self-synchronization mechanism of the other two vibrators is analyzed through small parameter average method and Routh-Hurwitz criterion. Besides, necessary calculation and simulation cases in multi-phase composite synchronization state are further conducted. Research results indicate that the multi-phase composite synchronization of three vibrators can be carried out in stable operation regions, but chaotic behaviors, since severe mass asymmetry of the eccentric rotors (ERs), will be induced when the phase differences (PDs) between two coaxial vibrators are controlled in -1.99â¼-1.67 and 1.63â¼2.1 (rad), and the controlled PDs around -0.4 (rad) should be avoid to ensure a stronger synchronization ability.
RESUMEN
Intranuclear protein delivery shows great prospects in broadening the application scope of protein therapy and revolutionizing medicine, however, effective delivery of native proteins into the nucleus of cells remains a great challenge. Herein, we report the supramolecular nanoparticles based on the self-assembly of dibenzocyclooctyne (DIBO) linked lysine-cyclodextrin (DLC) for efficient intranucleus delivery of proteins. Coordination-driven self-assembly of DLCs in aqueous solution enables efficient encapsulation of proteins just by simple mixing, so as to maintain their biological activity in a reliable way. DLC nanoparticles ensure effective intranuclear protein delivery for therapeutic applications and gene regulation. This rationally designed DIBO containing amino acid-cyclodextrin derivative allows the development of a convenient and universal nanoplatform for intranuclear delivery of native proteins.