RESUMEN
Accurate extraction of crop acreage is an important element of digital agriculture. This study uses Sentinel-2A, Sentinel-1, and DEM as data sources to construct a multidimensional feature dataset encompassing spectral features, vegetation index, texture features, terrain features, and radar features. The Relief-F algorithm is applied for feature selection to identify the optimal feature dataset. And the combination of deep learning and the random forest (RF) classification method is utilized to identify lilies in Qilihe District and Yuzhong County of Lanzhou City, obtain their planting structure, and analyze their spatial distribution characteristics in Gansu Province. The findings indicate that terrain features significantly contribute to ground object classification, with the highest classification accuracy when the number of features in the feature dataset is 36. The precision of the deep learning classification method exceeds that of RF, with an overall classification accuracy and kappa coefficient of 95.9% and 0.934, respectively. The Lanzhou lily planting area is 137.24 km2, and it primarily presents a concentrated and contiguous distribution feature. The study's findings can serve as a solid scientific foundation for Lanzhou City's lily planting structure adjustment and optimization and a basis of data for local lily yield forecasting, development, and application.
RESUMEN
BACKGROUND: Hepatocellular carcinoma (HCC) is a highly malignant tumor with high incidence and mortality rates. Aging-related genes are closely related to the occurrence and development of cancer. Therefore, it is of great significance to evaluate the prognosis of HCC patients by constructing a model based on aging-related genes. METHOD: Non-negative matrix factorization (NMF) clustering analysis was used to cluster the samples. The correlation between the risk score and immune cells, immune checkpoints, and Mismatch Repair (MMR) was evaluated through Spearman correlation test. Real Time Quantitative PCR (RT-qPCR) and immunohistochemistry were used to validate the expression levels of key genes in tissue and cells for the constructed model. RESULT: By performing NMF clustering, we were able to effectively group the liver cancer samples into two distinct clusters. Considering the potential correlation between aging-related genes and the prognosis of liver cancer patients, we used aging-related genes to construct a prognostic model. Spearman correlation analysis showed that the model risk score was closely related to MMR and immune checkpoint expression. Drug sensitivity analysis also provided guidance for the clinical use of chemotherapy drugs. RT-qPCR showed that TFDP1, NDRG1, and FXR1 were expressed at higher levels in different liver cancer cell lines compared to normal liver cells. CONCLUSION: In summary, we have developed an aging-related model to predict the prognosis of hepatocellular carcinoma and guide clinical drug treatment for different patients.
Asunto(s)
Carcinoma Hepatocelular , Neoplasias Hepáticas , Humanos , Carcinoma Hepatocelular/genética , Carcinoma Hepatocelular/terapia , Multiómica , Neoplasias Hepáticas/genética , Neoplasias Hepáticas/terapia , Pronóstico , Inmunoterapia , Aprendizaje Automático , Envejecimiento , Microambiente Tumoral , Proteínas de Unión al ARNRESUMEN
CD8+ regulatory T cells (Tregs) play an important role in regulating peripheral immune tolerance. However, difficulties in the characterization of CD8+ Tregs that lack suitable markers have a considerably limited research in this area. Moreover, the induction and effector mechanisms of CD8+ Tregs remain unclear. Herein, we demonstrate the suitability of Ly49 and CD44 as markers for CD8+ Tregs. Our data also show that MHC class II restricted peptides induce CD8+CD44+Ly49+ Tregs via CD4+ T cell activation. Furthermore, we also found cross-suppressive activity of these CD8+ Tregs on responding CD4+ T cells in a cytotoxicity dependent manner. Our data provide new insights into the induction and function of CD8+ Tregs.