Implementation and experimental validation of surface electromyogram and force model of Tibialis Anterior muscle for examining muscular factors.

This study reports a surface electromyogram and force of contraction model. The objective was to investigate the effect of changes in the size, type and number of motor units in the Tibialis Anterior muscle to surface electromyogram and force of dorsiflexion. A computational model to simulate surface electromyogram and associated force of contraction by the Tibialis Anterior muscle was developed. This model was simulated for isometric dorsiflexion, and comparative experiments were conducted for validation. Repeated simulations were performed to investigate the different parameters and evaluate inter-experimental variability. An equivalence statistical test and the Bland-Altman method were used to observe the significance between the simulated and experimental data. Simulated and experimentally recorded data had high similarity for the three measures: maximal power of power spectral density (p < 0.0001), root mean square of surface electromyogram (p < 0.0001) and force recorded at the footplate (p < 0.03). Inter-subject variability in the experimental results was in-line with the variability in the repeated simulation results. This experimentally validated computational model for the surface electromyogram and force of the Tibialis Anterior muscle is significant as it allows the examination of three important muscular factors associated with ageing and disease: size, fibre type and number of motor units.

Computational model to investigate the relative contributions of different neuromuscular properties of tibialis anterior on force generated during ankle dorsiflexion.

This study describes a new model of the force generated by tibialis anterior muscle with three new features: single-fiber action potential, twitch force, and pennation angle. This model was used to investigate the relative effects and interaction of ten age-associated neuromuscular parameters. Regression analysis (significance level of 0.05) between the neuromuscular properties and corresponding simulated force produced at the footplate was performed. Standardized slope coefficients were computed to rank the effect of the parameters. The results show that reduction in the average firing rate is the reason for the sharp decline in the force and other factors, such as number of muscle fibers, specific force, pennation angle, and innervation ratio. The fast fiber ratio affects the simulated force through two significant interactions. This study has ranked the individual contributions of the neuromuscular factors to muscle strength decline of the TA and identified firing rate decline as the biggest cause followed by decrease in muscle fiber number and specific force. The strategy for strength preservation for the elderly should focus on improving firing rate. Graphical abstract Neuromuscular properties of Tibialis Anterior on force generated during ankle dorsiflexion.

A computational model to investigate the effect of pennation angle on surface electromyogram of Tibialis Anterior.

This study has described and experimentally validated the differential electrodes surface electromyography (sEMG) model for tibialis anterior muscles during isometric contraction. This model has investigated the effect of pennation angle on the simulated sEMG signal. The results show that there is no significant effect of pennation angle in the range 0° to 20° to the single fibre action potential shape recorded on the skin surface. However, the changes with respect to pennation angle are observed in sEMG amplitude, frequency and fractal dimension. It is also observed that at different levels of muscle contractions there is similarity in the relationships with Root Mean Square, Median Frequency, and Fractal Dimension of the recorded and simulated sEMG signals.

A Facile and Flexible Method for On-Demand Directional Speed Tunability in the Miniaturised Lab-on-a-Disc.

The Miniaturised Lab-on-a-Disc (miniLOAD) platform, which utilises surface acoustic waves (SAWs) to drive the rotation of thin millimeter-scale discs on which microchannels can be fabricated and hence microfluidic operations can be performed, offers the possibility of miniaturising its larger counterpart, the Lab-on-a-CD, for true portability in point-of-care applications. A significant limitation of the original miniLOAD concept, however, is that it does not allow for flexible control over the disc rotation direction and speed without manual adjustment of the disc's position, or the use of multiple devices to alter the SAW frequency. In this work, we demonstrate the possibility of achieving such control with the use of tapered interdigitated transducers to confine a SAW beam such that the localised acoustic streaming it generates imparts a force, through hydrodynamic shear, at a specific location on the disc. Varying the torque that arises as a consequence by altering the input frequency to the transducers then allows the rotational velocity and direction of the disc to be controlled with ease. We derive a simple predictive model to illustrate the principle by which this occurs, which we find agrees well with the experimental measurements.

Age-Associated Changes in the Spectral and Statistical Parameters of Surface Electromyogram of Tibialis Anterior.

Age-related neuromuscular change of Tibialis Anterior (TA) is a leading cause of muscle strength decline among the elderly. This study has established the baseline for age-associated changes in sEMG of TA at different levels of voluntary contraction. We have investigated the use of Gaussianity and maximal power of the power spectral density (PSD) as suitable features to identify age-associated changes in the surface electromyogram (sEMG). Eighteen younger (20-30 years) and 18 older (60-85 years) cohorts completed two trials of isometric dorsiflexion at four different force levels between 10% and 50% of the maximal voluntary contraction. Gaussianity and maximal power of the PSD of sEMG were determined. Results show a significant increase in sEMG's maximal power of the PSD and Gaussianity with increase in force for both cohorts. It was also observed that older cohorts had higher maximal power of the PSD and lower Gaussianity. These age-related differences observed in the PSD and Gaussianity could be due to motor unit remodelling. This can be useful for noninvasive tracking of age-associated neuromuscular changes.

Effect of toe extension on EMG of triceps surae muscles during isometric dorsiflexion.

The protocol for estimating force of contraction by triceps surae (TS) muscles requires the immobilization of the ankle during dorsiflexion and plantar flexion. However, large variability in the results has been observed. To identify the cause of this variability, experiments were conducted where ankle dorsiflexion force and electromyogram (EMG) of the TS were recorded under two conditions: (i) toes were strapped and (ii) toes were unstrapped, with all other conditions such as immobilization of the ankle remaining unchanged. The root mean square (RMS) of the EMG and the force were analyzed and one-tail Student's t-test was performed for significance between the two conditions. The RMS of the EMG from TS muscles was found to be significantly higher (~55%) during dorsiflexion with toes unstrapped compared with when the toes were strapped. The torque corresponding to dorsiflexion was also higher with toes unstrapped. Our study has shown that it is important to strap the toes when measuring the torque at the ankle and EMG of the TS muscles.

Effect of number of motor units and muscle fibre type on surface electromyogram.

Reduction in number of motor units (nMU) and fast fibre ratio (FFR) is associated with disease or atrophy when this is rapid. There is a need to study the effect of nMU and FFR to analyse the association with ageing and disease. This study has developed a mathematical model to investigate the relationship between nMU and FFR on surface electromyogram (sEMG) of the biceps muscles. The model has been validated by comparing the simulation outcomes with experiments comparing the sEMG of physically active younger and older cohort. The results show that there is statistically significant difference between the two groups, and the simulation studies closely model the experimental results. This model can be applied to identify the cause of muscle weakness among the elderly due to factors such as muscle dystrophy or preferential loss of type F muscle fibres.

Age related neuromuscular changes in sEMG of m. Tibialis Anterior using higher order statistics (Gaussianity & linearity test).

Age-associated changes in the surface electromyogram (sEMG) of Tibialis Anterior (TA) muscle can be attributable to neuromuscular alterations that precede strength loss. We have used our sEMG model of the Tibialis Anterior to interpret the age-related changes and compared with the experimental sEMG. Eighteen young (20-30 years) and 18 older (60-85 years) performed isometric dorsiflexion at 6 different percentage levels of maximum voluntary contractions (MVC), and their sEMG from the TA muscle was recorded. Six different age-related changes in the neuromuscular system were simulated using the sEMG model at the same MVCs as the experiment. The maximal power of the spectrum, Gaussianity and Linearity Test Statistics were computed from the simulated and experimental sEMG. A correlation analysis at α=0.05 was performed between the simulated and experimental age-related change in the sEMG features. The results show the loss in motor units was distinguished by the Gaussianity and Linearity test statistics; while the maximal power of the PSD distinguished between the muscular factors. The simulated condition of 40% loss of motor units with halved the number of fast fibers best correlated with the age-related change observed in the experimental sEMG higher order statistical features. The simulated aging condition found by this study corresponds with the moderate motor unit remodelling and negligible strength loss reported in literature for the cohorts aged 60-70 years.

Improvement of isometric dorsiflexion protocol for assessment of tibialis anterior muscle strength.

It is important to accurately estimate the electromyogram (EMG)/force relationship of triceps surae (TS) muscle for detecting strength deficit of tibalis anterior (TA) muscle. In literature, the protocol for recording EMG and force of dorsiflexion have been described, and the necessity for immobilizing the ankle has been explained. However, there is a significant variability of the results among researchers even though they report the fixation of the ankle. We have determined that toe extension can cause significant variation in the dorsiflexion force and EMG of TS and this can occur despite following the current guidelines which require immobilizing the ankle. The results also show that there was a large increase in the variability of the force and the RMS of EMG of TS when the toes were not strapped compared with when they were strapped. Thus, with the current guidelines, where there are no instructions regarding the necessity of strapping the toes, the EMG/force relationship of TS could be incorrect and give an inaccurate assessment of the dorsiflexor TA strength. In summary, •Current methodology to estimate the dorsiflexor TA strength with respect to the TS activity, emphasizing on ankle immobilization is insufficient to prevent large variability in the measurements.•Toe extension during dorsiflexion was found to be one source of variability in estimating the TA strength.•It is recommended that guidelines for recording force and EMG from TA and TS muscles should require the strapping of the toes along with the need for immobilizing the ankle.

Age-related motor unit remodeling in the Tibialis Anterior.

Limited studies exist on the use of surface electromyogram (EMG) signal features to detect age-related motor unit remodeling in the Tibialis Anterior. Motor unit remodeling leads to declined muscle strength and force steadiness during submaximal contractions which are factors for risk of falls in the elderly. This study investigated the remodeling phenomena in the Tibialis Anterior using sample entropy and higher order statistics. Eighteen young (26.1 ± 2.9 years) and twelve elderly (68.7 ± 9.0 years) participants performed isometric dorsiflexion of the ankle at 20% maximal voluntary contraction (MVC) and their Tibialis Anterior (TA) EMG was recorded. Sample entropy, Gaussianity and Linearity Test statistics were calculated from the recorded EMG for each MVC. Shapiro-Wilk test was used to determine normality, and either a two-tail student t-test or Wilcoxon rank sum test was performed to determine significant difference in the EMG features between the young and old cohorts. Results show age-related motor unit remodeling to be depicted by decreased sample entropy (p <; 0.1), increased non-Gaussianity (p <; 0.05) and lesser degree of linearity in the elderly. This is due to the increased sparsity of the MUAPs as a result of the denervation-reinnervation process, and the decrease in total number of motor units.

A model for generating Surface EMG signal of m. Tibialis Anterior.

A model that simulates surface electromyogram (sEMG) signal of m. Tibialis Anterior has been developed and tested. This has a firing rate equation that is based on experimental findings. It also has a recruitment threshold that is based on observed statistical distribution. Importantly, it has considered both, slow and fast type which has been distinguished based on their conduction velocity. This model has assumed that the deeper unipennate half of the muscle does not contribute significantly to the potential induced on the surface of the muscle and has approximated the muscle to have parallel structure. The model was validated by comparing the simulated and the experimental sEMG signal recordings. Experiments were conducted on eight subjects who performed isometric dorsiflexion at 10, 20, 30, 50, 75, and 100% maximal voluntary contraction. Normalized root mean square and median frequency of the experimental and simulated EMG signal were computed and the slopes of the linearity with the force were statistically analyzed. The gradients were found to be similar (p>0.05) for both experimental and simulated sEMG signal, validating the proposed model.

Specific collection of adherent cells using laser release in a droplet-driven capillary cell.

Laser pressure catapulting of adherent cells directly or cells grown on micropallets are two common methods of dislodgement. We describe a method where laser catapulting is performed as a flow is introduced orthogonally in a simple capillary chamber that is inexpensive and obviates flow-generating devices. The moving cells terminate near the contact line within the liquid medium, ensuring that they remain continuously hydrated and where the surface-tension forces hold them in place to permit a later collection process with a receptacle. By dislodging the cells close to the free edge of the liquid chamber, the amount of cell travel and, thus, contamination is minimized. The metrics of cell death and movement show that firing of the laser beam center a distance away from the cell to create a bubble that cavitates over time is more viable with the technique than directly on the cell.