Ensemble and single-particle level fluorescent fine-tuning of carbon dots via positional changes of amines toward "supervised" oral microbiome sensing.

Significance: Carbon dots (CDs) have attracted a host of research interest in recent years mainly due to their unique photoluminescence (PL) properties that make them applicable in various biomedical areas, such as imaging and image-guided therapy. However, the real mechanism underneath the PL is a subject of wide controversy and can be investigated from various angles. Aim: Our work investigates the effect of the isomeric nitrogen position as the precursor in the synthesis of CDs by shedding light on their photophysical properties on the single particles and ensemble level. Approach: To this end, we adopted five isomers of diaminopyridine (DAP) and urea as the precursors and obtained CDs during a hydrothermal process. The various photophysical properties were further investigated in depth by mass spectroscopy. CD molecular frontier orbital analyses aided us in justifying the fluorescence emission profile on the bulk level as well as the charge transfer processes. As a result of the varying fluorescent responses, we indicate that these particles can be utilized for machine learning (ML)-driven sensitive detection of oral microbiota. The sensing results were further supported by density functional theoretical calculations and docking studies. Results: The generating isomers have a significant effect on the overall photophysical properties at the bulk/ensembled level. On the single-particle level, although some of the photophysical properties such as average intensity remained the same, the overall differences in brightness, photo-blinking frequency, and bleaching time between the five samples were conceived. The various photophysical properties could be explained based on the different chromophores formed during the synthesis. Overall, an array of CDs was demonstrated herein to achieve ∼100% separation efficacy in segregating a mixed oral microbiome culture in a rapid (<0.5 h), high-throughput manner with superior accuracy. Conclusions: We have indicated that the PL properties of CDs can be regulated by the precursors' isomeric position of nitrogen. We emancipated this difference in a rapid method relying on ML algorithms to segregate the dental bacterial species as biosensors.

Influence of physical fitness of coronary disease patients on vagal reentry and heart rate recovery after exercise with and without fluid replacement.

BACKGROUND: Fluid replacement during exercise has been studied as an important strategy to enhance recovery, however, studies are needed to investigate the effect of this strategy in different physical profiles. The aim of the study was to investigate the influence of physical fitness of coronary artery disease (CAD) patients on vagal reentry and heart rate recovery after exercise performed with and without fluid replacement. METHODS: Nonrandomized crossover clinical trial. Thirty-three CAD patients were submitted to (I) cardiopulmonary exercise test: to divide the sample into lower and higher VO2  peak groups; (II) control protocol (CP): composed of rest, aerobic exercise and passive recovery; (III) hydration protocol (HP): composed of the same activities as the CP, but with water intake during exercise. The recovery was evaluated by vagal reentry and heart rate recovery immediately after exercise. RESULTS: The results did not show significant differences between the higher and lower VO2  peak groups. In addition, the hydration strategy adopted was not able to cause significant changes between control and HPs, regardless of group. However, a time effect was observed, suggesting anticipation of vagal reactivation and heart rate reduction in HP. CONCLUSIONS: Physical fitness did not influence vagal reentry and heart rate recovery in CAD patients after exercise. However, the hydration strategy seems to have anticipated vagal reentry and produced a more efficient reduction in heart rate regardless of the individuals' physical fitness, but these results should be analyzed with caution due to the absence of significant differences between groups and protocols.

Cymbopogon citratus Protects Erythrocytes from Lipid Peroxidation in vitro.

INTRODUCTION: Essential oils are complex mixtures of volatile compounds, primarily composed of terpenes and abundant aromatic plants. For example, Cymbopogon citratus (lemongrass) is an aromatic plant that produces a monoterpene-rich essential oil, and studies show that this essential oil has excellent antioxidant activity. Erythrocytes incubated under high sugar levels are constantly exposed to reactive oxygen species, which results in the oxidation of their membranes. OBJECTIVE: The aim of this article is to investigate the antioxidant activity of lemongrass essential oil and its protective effect on erythrocytes exposed to high levels of glucose. MATERIALS AND METHODS: The essential oil was obtained by steam dragging distillation; blood cell suspensions were incubated with glucose 5, 20, 50, and 100 mmol/L. The amount of TBARS produced was measured at 532 nm. In addition, the percentage of antioxidant activity was assessed by DPPH free radical assay. RESULTS: Lemongrass essential oil showed an increase in the antioxidant activity up to 240 mg/ml, while ascorbic acid used as positive control showed a dose-dependent increase in antioxidant activity starting at 1 mmol/L up to 18 mmol/L. However, such a lemongrass dose prevented peroxidation in erythrocytes incubated under a high glucose media, whereas ascorbic acid showed a protective effect only at a concentration of 1 mmol/L. CONCLUSION: Lemongrass essential oil has high antioxidant activity compared to standard antioxidant as ascorbic acid, and also acts as a protective agent against erythrocyte lipoperoxidation due to hyperglycemia in vitro.

Dance therapy as an alternative for cardiac rehabilitation in women population: autonomic and hemodynamic acute responses - a crossover clinical trial protocol

Abstract Aim: The objective of this study is to 1. Investigate in women with cardiac risk factors the acute responses of cardiac autonomic modulation and hemodynamic parameters during and after a dance-based cardiac rehabilitation session and II. Compare these responses with a conventional exercise-based cardiac rehabilitation session. Methods: This will be a crossover-clinical trial that will enroll women with at least one cardiac risk factor. The interventions consist of one dance-based (DB) and one traditional exercise-based (EB) session of cardiac rehabilitation, both composed of initial rest, warm-up, moderate-intensity physical exercise, fast recovery evaluation in orthostatic position, and slow recovery evaluation. The main outcomes are 1. Autonomic modulation, evaluated through heart rate variability linear and non-linear methods, and II. Hemodynamic parameters (heart rate, blood pressure, peripheral oxygen saturation, and respiratory rate). All measures will be evaluated in specific moments during the initial rest, physical exercise, and recovery. Conclusion: The results will allow the safe inclusion of dance-based sessions in cardiac rehabilitation programs opening an important field of research to investigate the long-term effects on physical fitness and cardiac risk factors, as well as adherence and motivation to attend cardiac rehabilitation in the women population.

Gastric Pneumatosis: An Atypical Presentation of Desmoid Tumor.

CASE PRESENTATION: A middle-aged woman presented to the emergency department with a chief complaint of abdominal pain, fever, vomiting, and diarrhea. Abdominal computed tomography revealed gastric pneumatosis and air in the portal system. The patient had an unfavorable clinical course with pneumoperitoneum and septic shock due to secondary peritonitis. She underwent emergency laparotomy where neoformation of the mesentery root was found with infiltration of the small intestine and jejunal perforation. The anatomopathological study of the tumor revealed that it was a desmoid tumor. DISCUSSION: To our knowledge this is the first report in the literature of gastric pneumatosis as the initial presentation of a mesenteric desmoid tumor. Although it usually has a benign clinical course, its locally invasive characteristics can lead to critical illness. Physicians shouldn't overlook these types of complications, as early identification and surgical intervention can modify the prognosis and shorten hospital stay.

Modulation of π-stacking modes and photophysical properties of an organic semiconductor through isosteric cocrystallization.

We report on the control of π-stacking modes (herringbone vs slipped-stack) and photophysical properties of 9,10-bis((E)-2-(pyridin-4-yl)vinyl)anthracene (BP4VA), an anthracene-based organic semiconductor (OSC), by isosteric cocrystallization (i.e., the replacement of one functional group in a coformer with another of "similar" electronic structure) with 2,4,6-trihalophenols (3X-ph-OH, where X = Cl, Br, and I). Specifically, BP4VA organizes as slipped-stacks when cocrystallized with 3Cl-ph-OH and 3Br-ph-OH, while cocrystallization with 3I-ph-OH results in a herringbone mode. The photoluminescence and molecular frontier orbital energy levels of BP4VA were effectively modulated by the presence of 3X-ph-OH through cocrystallization. We envisage that the cocrystallization of OSCs with minimal changes in cocrystal formers can provide access to convenient structural and property diversification for advanced single-crystal electronics.

Trypanocidal activity of new 1,6-diphenyl-1H-pyrazolo[3,4-b]pyridine derivatives: Synthesis, in vitro and in vivo studies.

Despite the serious public health problems caused by Chagas disease in several countries, the available therapy remains with only two drugs that are poorly active during the chronic phase of the disease in addition to having severe side effects. In search of new trypanocidal agents, herein we describe the synthesis and biological evaluation of eleven new 1,6-diphenyl-1H-pyrazolo[3,4-b]pyridine compounds containing the carbohydrazide or the 2,3-dihydro-1,3,4-oxadiazole moieties. Two of them showed promising in vitro activity against amastigote forms of T. cruzi and were evaluated in vivo in male BALB/c mice infected with T. cruzi Y strain. Our results suggest that the substitution at the C-2 position of the phenyl group connected to the carbohydrazide or to the 2,3-dihydro-1,3,4-oxadiazole moieties plays an important role in the trypanocidal activity of this class of compounds. Moreover, the compound containing the 2,3-dihydro-1,3,4-oxadiazole moiety has demonstrated more favorable structural requirements for in vivo activity than its carbohydrazide analog.

Aprendizagem escolar: desafios do professor na atividade docente / School Learning: teacher's challenges in their teaching activities / Aprendizaje Escolar: desafíos del profesor en la actividad docente

Nas duas últimas décadas, período este delimitado a partir da promulgação da atual Lei de Diretrizes e Bases da Educação Nacional (Lei 9.394/96) e marcado por uma elevação na quantidade de matrículas na escola, especialmente no ensino fundamental, o processo de aprendizagem escolar tem se constituído numa questão urgente do nosso tempo, seja nos cursos de formação inicial e/ou continuada de professores, seja no seio da família e da própria escola, ou ainda nos consultórios médicos. Diante dessa problemática, a atividade docente tem sido analisada a partir de diferentes perspectivas, tanto políticas quanto pedagógicas, com vistas a atender a questão do processo de aprendizagem escolar. Tendo a psicologia sócio-histórica como base teórico-metodológica, este artigo objetiva apreender a dimensão subjetiva dos desafios vividos pelo professor na atividade docente frente às possibilidades de aprendizagem que se configuram no espaço escolar. Metodologicamente, o trabalho de investigação se desenvolveu por meio da realização de entrevista semiestruturada e o processo de análise e interpretação dos dados teve como base de apoio a proposta dos núcleos de significação. Os resultados do processo de análise e interpretação apontam para a constituição de dois núcleos de significação, a saber: 1) A vida na escola: um caminho de flores e espinhos; e 2) Da formação à prática: sonhos, possibilidades e desafios.

The last two decades was a period delimited by the promulgation of the current Lei de Diretrizes e Bases da Educação Nacional (Lei 9.394/96) - National Education Guidelines and Base Law (Law 9,394/96), and characterized by an increase in the number of registrations in schools, especially in regards to elementary and middle schools. The school learning process has been constituted as an urgent issue for our time, whether in the undergraduate course and/or other academic training for teachers, or in the family and school environments or even in the doctors' offices. Based on this matter, the teaching activity has been analyzed through different perspectives, both political and pedagogical, in view of addressing the issue of school learning process. Taking into account the socio-historical psychology as theoretical and methodological basis, this article aims to seize the subjective dimension of the challenges experienced by the teacher in their teaching activities as pertaining to the students' learning. Methodologically, the research was developed through semi-structured interview and the process of analysis and interpretation of the data was based on the nuclei of meanings proposal. The results of the process of analysis and interpretation points out to a constitution of two nuclei of meanings, they are: 1) The school life: a path of flowers and thorns; and 2) From training to practice: challenges and possibilities.

En las últimas dos décadas, período delimitado desde la promulgación actual de la Ley de Directrices e Base de la Educación Nacional (Ley 9.394/96), y marcado por un aumento en el número de inscripciones en la escuela, especialmente en la enseñanza mediana, el proceso de aprendizaje se ha constituido en una cuestión urgente de nuestro tempo, en cursos de formación inicial y continua de profesores, dentro de la familia o en consultorios médicos. Frente a esta problemática, la actividad docente ha sido analizada desde diferentes perspectivas, tanto políticas como educativas. Teniendo la psicología socio-histórica como base teórico-metodológica, este artículo surge con el objetivo de apoderarse de la dimensión subjetiva de las dificultades experimentadas por el profesor en la enseñanza de la actividad, frente al estudiante de la escuela de aprendizaje. Metodológicamente, el trabajo de investigación desarrollado por medio de entrevista semiestructurada y el proceso de análisis e interpretación de los datos se basó en la propuesta de la ayuda del núcleo de significación. Los resultados del proceso de análisis e interpretación presentan la constitución de dos núcleos de significado, son ellos: 1) La vida en la escuela: "cada vez se encuentra una flor" y 2) Desafíos de formación: "Creemos que la escuela puede cambiar el mundo".

Multimodal characterization of the bone-implant interface using Raman spectroscopy and nanoindentation.

Titanium implants are widely used in dental and orthopedic surgeries. Osseointegration phenomena lead to direct contact between bone tissue and the implant surface. The quality of the bone-implant interface (BII), resulting from the properties of newly formed bone, determines the implant stability. This study investigates the BII properties using a dedicated in vivo implant model consisting of a coin-shaped Ti-6Al-4V implant inserted in a rabbit femur for 10 weeks. A gap created below the implant was filled with newly formed bone tissue after healing. The properties of mature and newly formed bone tissues were compared using: i) Raman spectroscopy to assess the nanoscale compositional bone properties and ii) nanoindentation to quantify microscale elastic properties in site-matched regions. The mineral-to-matrix ratio, crystallinity (mineral size and lattice order), and the collagen cross-link ratio were significantly lower in newly formed bone tissue (e.g., a mineral-to-matrix ratio of 9.3 ± 0.5 for proline 853 cm-1) compared to mature bone (15.6 ± 1). Nanoindentation measurements gave Young's modulus of 12.8 ± 1.8 GPa for newly formed bone and 15.7 ± 2.3 GPa for mature bone. This multimodal and multiscale approach leads to a better understanding of osseointegration phenomena.

Complementary Oligonucleotide Conjugated Multicolor Carbon Dots for Intracellular Recognition of Biological Events.

By using complementary DNA sequences as surface ligands, we selectively allow two individual diffusing "dual-color" carbon dots to interact in situ and in vitro. Spontaneous nanoscale oxidation of surface-abundant nitroso-/nitro-functionalities leads to two distinctly colored carbon dots (CD) which are isolated by polarity driven chromatographic separation. Green- and red-emitting carbon dots (gCD and rCD) were decorated by complementary single-stranded DNAs which produce a marked increase in the fluorescence emission of the respective carbon dots. Mutual colloidal interactions are achieved through hybridization of complementary DNA base pairs attached to the respective particles, resulting in quenching of their photoluminescence. The observed post-hybridization quenching is presumably due to a combined effect from an aggregation of CDs post duplex DNA formation and close proximity of multicolored CDs, having overlapped spectral regions leading to a nonradiative energy transfer process possibly released as heat. This strategy may contribute to the rational design of mutually interacting carbon dots for a better control over the resulting assembly structure for studying different biological phenomenon including molecular cytogenetics. One of the newly synthesized CDs was successfully used to image intracellular location of GAPDH mRNA using an event of change in fluorescence intensity (FI) of CDs. This selectivity was introduced by conjugating an oligonucleotide harboring complementary sequence to GAPDH mRNA. FI of this conjugated carbon dot, rCD-GAPDH, was also found to decrease in the presence of Ca2+, varied in relation to H+ concentrations, and could serve as a tool to quantify the intracellular concentrations of Ca2+ and pH value (H+) which can give important information about cell survival. Therefore, CD-oligonucleotide conjugates could serve as efficient probes for cellular events and interventions.

Oligodots: Structurally Defined Fluorescent Nanoprobes for Multiscale Dual-Color Imaging in Vitro and in Vivo.

Nanoscale fluorescent probes are of great importance due to their capabilities for imaging on multiscale. Herein, we report the first synthesis of structurally well-defined nanoparticulate "oligodots" developed for multicolor imaging in vitro and in vivo. These nanoparticles are prepared via condensation and curing reactions where the engineering of the solvent results in the nanoparticles with green (λem = 550 nm) and red (λem = 650 nm) emission range. Differences found in the photophysical properties have been attributed to variations in oligomeric compositions produced during the synthesis as was corroborated by extensive physicochemical characterizations. Specifically, mass spectroscopy provided a picture of the formed species during the synthesis. The feasibility of the oligodots for multicolor imaging is demonstrated both in vitro and in vivo. The red-emitting oligodot is employed for dynamic whole-body imaging in mice. It is envisioned that oligodots would enable multicolor imaging of various biomarkers in complex diseases such as cancer where numerous molecular and metabolic phenotypes work in concert in their emergence.

Carbon dots with induced surface oxidation permits imaging at single-particle level for intracellular studies.

For robust single particle optical detection, a high sensitivity in photoluminescence (PL) of Carbon Dots (CDs) must be achieved. PL sensitivity can be successfully correlated with their surface chemistry but requires high synthetic control without altering their basic surface properties. Here we describe conditions for the controlled synthesis of CDs that resulted in a PL sensitivity at the single-particle level. We report that a stoichiometric catalyst N-methyl morpholine-N-oxide (NMMO) can be used as a 'sacrificial' single additive to aid nanoscale surface oxidation. A 24 h NMMO-mediated oxidation increased coverage of oxidized nanoscale surface 3% to 20.9%. NMMO-oxidized CDs (CD-NMMOs) display superior particle brightness, as evidenced by the increase of light absorbance and an enhancement of quantum yield which is characterized by a series of physicochemical and biophysical experiments. We also demonstrate that CD-NMMOs is well suited for intracellular and single-particle imaging.

Three-Dimensional Multiscale, Multistable, and Geometrically Diverse Microstructures with Tunable Vibrational Dynamics Assembled by Compressive Buckling.

Microelectromechanical systems remain an area of significant interest in fundamental and applied research due to their wide ranging applications. Most device designs, however, are largely two-dimensional and constrained to only a few simple geometries. Achieving tunable resonant frequencies or broad operational bandwidths requires complex components and/or fabrication processes. The work presented here reports unusual classes of three-dimensional (3D) micromechanical systems in the form of vibratory platforms assembled by controlled compressive buckling. Such 3D structures can be fabricated across a broad range of length scales and from various materials, including soft polymers, monocrystalline silicon, and their composites, resulting in a wide scope of achievable resonant frequencies and mechanical behaviors. Platforms designed with multistable mechanical responses and vibrationally de-coupled constituent elements offer improved bandwidth and frequency tunability. Furthermore, the resonant frequencies can be controlled through deformations of an underlying elastomeric substrate. Systematic experimental and computational studies include structures with diverse geometries, ranging from tables, cages, rings, ring-crosses, ring-disks, two-floor ribbons, flowers, umbrellas, triple-cantilever platforms, and asymmetric circular helices, to multilayer constructions. These ideas form the foundations for engineering designs that complement those supported by conventional, microelectromechanical systems, with capabilities that could be useful in systems for biosensing, energy harvesting and others.

Self-Assembled, Nanostructured, Tunable Metamaterials via Spinodal Decomposition.

Self-assembly via nanoscale phase separation offers an elegant route to fabricate nanocomposites with physical properties unattainable in single-component systems. One important class of nanocomposites are optical metamaterials which exhibit exotic properties and lead to opportunities for agile control of light propagation. Such metamaterials are typically fabricated via expensive and hard-to-scale top-down processes requiring precise integration of dissimilar materials. In turn, there is a need for alternative, more efficient routes to fabricate large-scale metamaterials for practical applications with deep-subwavelength resolution. Here, we demonstrate a bottom-up approach to fabricate scalable nanostructured metamaterials via spinodal decomposition. To demonstrate the potential of such an approach, we leverage the innate spinodal decomposition of the VO2-TiO2 system, the metal-to-insulator transition in VO2, and thin-film epitaxy, to produce self-organized nanostructures with coherent interfaces and a structural unit cell down to 15 nm (tunable between horizontally and vertically aligned lamellae) wherein the iso-frequency surface is temperature-tunable from elliptic to hyperbolic dispersion producing metamaterial behavior. These results provide an efficient route for the fabrication of nanostructured metamaterials and other nanocomposites for desired functionalities.

Effects of Red Wine Tannat on Oxidative Stress Induced by Glucose and Fructose in Erythrocytes in Vitro.

The literature indicates that red wine presents in its composition several substances that are beneficial to health. This study has investigated the antioxidant effects of Tannat red wine on oxidative stress induced by glucose and fructose in erythrocytes in vitro, with the purpose to determine some of its majoritarian phenolic compounds and its antioxidant capacity. Erythrocytes were incubated using different concentrations of glucose and fructose in the presence or absence of wine. From these erythrocytes were determined the production of thiobarbituric acid reactive species (TBARS), glucose consumption, and osmotic fragility. Moreover, quantification of total phenolic, gallic acid, caffeic acid, epicatechin, resveratrol, and DPPH scavenging activity in wine were also assessed. Red wine showed high levels of polyphenols analyzed, as well as high antioxidant potential. Erythrocytes incubated with glucose and fructose had an increase in lipid peroxidation and this was prevented by the addition of wine. The wine increased glucose uptake into erythrocytes and was able to decrease the osmotic fragility of erythrocytes incubated with fructose. Altogether, these results suggest that wine leads to a reduction of the oxidative stress induced by high concentrations of glucose and fructose.

Ebselen exhibits glycation-inhibiting properties and protects against osmotic fragility of human erythrocytes in vitro.

Diabetic status is associated with an increase on oxidative stress markers in humans and animal models. We have investigated the in vitro effects of high concentrations of glucose on the profile of oxidative stress and osmotic fragility of blood from control and diabetic patients; we considered whether its antioxidant properties could afford some protection against glucose-induced osmotic fragility, and whether ebselen could act as an inhibitor of hemoglobin glycation. Raising blood glucose to 5-100 mmol/L resulted in a concentration-dependent increase of glycated hemoglobin (HbA1c; P < 0.001) and thiobarbituric acid reactive species (TBA-RS) content (P < 0.004). Non-protein SH groups (NPSH) also increased significantly as the concentration of glucose increased up to 30 mmol/L (P < 0.001). The osmotic fragility was more pronounced in blood of uncontrolled diabetic patients than in these non-diabetic subjects. Ebselen significantly reduced the glucose-induced increase in osmotic fragility and inhibited HbA1c formation (P < 0.0001). These results indicate that blood from patients with uncontrolled diabetes are more sensitive to osmotic shock than from patients with controlled diabetes and control subjects in relation to increased production of free radicals in vivo.

A formação de uma professora do ensino fundamental: contribuições da psicologia sócio-histórica / The preparation of a basic school teacher: contributions of the social-historical approach in Psychology

O objetivo deste trabalho é discutir os sentidos e significados de uma professora do ensino fundamental acerca da sua formação em serviço. Metodologicamente, utilizou-se a proposta de núcleos de significação, cujo processo de apreensão de sentidos e significados ocorre por meio da sistematização de pré-indicadores, indicadores e núcleos de significação. Utilizaram-se entrevistas recorrentes com base na fala e em fontes documentais do sujeito. Analisou-se apenas a mediação afetiva na constituição do ser professora. Esse revela aspectos da formação do sujeito articulados à sua docência e ao seu modo de ser professora(AU)

The goal of this study is to discuss the meanings attributed by a basic schooling teacher to her own in-service training. The method employed was the one proposed usually know as "signification centers construction", which tries to grasp the meanings attributed to facts, events and/or situations by focused individuals or groups, through a systematic elaboration of 'pre-indicators', 'indicators' and 'signification centers'. Recurrent interviews and analysis of the subject's available documents. In this paper, however, only one of these centers is fully explained affective mediation and its role in the process of becoming a teacher. That it allowed a better understanding about how the teacher articulated her training to her practice, i. e, to her way of acting as a teacher(AU)

El objetivo de este trabajo es discutir los sentidos y significados de una profesora de la enseñanza primaria sobre su formación en servicio. Metodológicamente se utilizó la propuesta de núcleos de significado, cuyo proceso de aprehensión de sentidos y significados ocurre por medio de la sistematización de pre-indicadores, indicadores y núcleos de significado. Se utilizaron entrevistas recurrentes con base en el habla y en fuentes documentales del sujeto. Se analizó apenas la mediación afectiva en la constitución del ser profesora. Eso revela aspectos de la formación del sujeto articulados a su docencia y a su modo de ser como profesora(AU)

A formação de uma professora do ensino fundamental: contribuições da psicologia sócio-histórica / The preparation of a basic school teacher: contributions of the social-historical approach in Psychology

O objetivo deste trabalho é discutir os sentidos e significados de uma professora do ensino fundamental acerca da sua formação em serviço. Metodologicamente, utilizou-se a proposta de núcleos de significação, cujo processo de apreensão de sentidos e significados ocorre por meio da sistematização de pré-indicadores, indicadores e núcleos de significação. Utilizaram-se entrevistas recorrentes com base na fala e em fontes documentais do sujeito. Analisou-se apenas a mediação afetiva na constituição do ser professora. Esse revela aspectos da formação do sujeito articulados à sua docência e ao seu modo de ser professora.

The goal of this study is to discuss the meanings attributed by a basic schooling teacher to her own in-service training. The method employed was the one proposed usually know as "signification centers construction", which tries to grasp the meanings attributed to facts, events and/or situations by focused individuals or groups, through a systematic elaboration of 'pre-indicators', 'indicators' and 'signification centers'. Recurrent interviews and analysis of the subject's available documents. In this paper, however, only one of these centers is fully explained affective mediation and its role in the process of becoming a teacher. That it allowed a better understanding about how the teacher articulated her training to her practice, i. e, to her way of acting as a teacher.

El objetivo de este trabajo es discutir los sentidos y significados de una profesora de la enseñanza primaria sobre su formación en servicio. Metodológicamente se utilizó la propuesta de núcleos de significado, cuyo proceso de aprehensión de sentidos y significados ocurre por medio de la sistematización de pre-indicadores, indicadores y núcleos de significado. Se utilizaron entrevistas recurrentes con base en el habla y en fuentes documentales del sujeto. Se analizó apenas la mediación afectiva en la constitución del ser profesora. Eso revela aspectos de la formación del sujeto articulados a su docencia y a su modo de ser como profesora.