[Activity of aminopeptidase and inter-alpha-trypsin inhibitor in serum of women in physiological labor]. / Aktywnosc aminopeptydazowa i stezenie inter-alfa-inhibitora trypsyny w surowicy krwi kobiet w okresach porodu fizjologicznego.

Aminopeptidase activity was measured towards L-alanine and L-leucine naphtylamides in the blood serum obtained from the non-pregnant women and from women during the stages of the physiological delivery. In the same samples the concentration of inter-alpha-trypsin inhibitor (alpha ITI) was measured. The highest aminopeptidase activity was observed in the stage II, and it was statistically significant comparing to the stage I, whereas the statistically significant increase in the alpha ITI concentration was noticed in stage I in primiparae and in stage I in multiparae.

[Urinary trypsin inhibitor in pregnant women with normal and pathological course of pregnancy]. / Inhibitor trypsyny w moczu kobiet w ciazy fizjologicznej i patologicznej.

Urinary trypin inhibitor concentration (UTI) was measured by Fritz and all method in the groups of women: non-pregnant (I), pregnant (II), in pregnancy complicated by EPH-gestosis (III) and in the prolongated pregnancy (IV). Furthermore, in the urine from the investigated group the index protein/creatinine was established. There was noticed the statistically significant increase in the UTI concentration in II, III, IV groups comparing to the non-pregnant group--I, and the increase in UTI in III and IV group in comparison to group II. The UTI measurement in the pregnant women connected with the index protein/creatinine could be significant for diagnosing of the pathology of pregnancy.

[Aminopeptidases activity in the amniotic fluid in women in labor with EPH-gestosis]. / Aktywnosc aminopeptydazowa w plynie owodniowym rodzacych z EPH-gestoza.

The thesis of this work was to compare the aminopeptidases activity in the amniotic fluid obtained during the physiological labor and during the labor of pregnant with EPH-gestosis, in presence of the beta-naphtlamidic L-amino-acids (alanine, leucine, phenylalanine, tyrosine, histidine, cysteine) chromogenic substrates. It was assumed the 3-7 times increase in the aminopeptidases activity counted to the proteins from the labored with EPH-gestosis comparing to the labored in physiological labor. Among the used substrates the highest activity in both groups of labored women was measured in presence of substrates with exact amino-acids in this order: L-Ala > L-Leu > L-Phe > L-Tyr > L-His > L-Cys.

[Concentration of cysteine proteinase inhibitors in urine, amniotic fluid and serum from women in pregnancy complicated by EPH-gestosis]. / Stezenie inhibitorów proteinaz cysteinowych w moczu, plynie owodniowym i surowicy kobiet w ciazy powiklanej EPH-gestoza.

Cysteine proteinase inhibitors (IPC) concentration was measured by the modified Barrett method using papaine in urine, amniotic fluid and serum obtained from the healthy labored women and from labored women in pregnancy complicated by EPH-gestosis. It was noticed the statistically significant increase in the IPC concentration in the material from the pregnant women with EPH-gestosis comparing to the women, which pregnancy had the physiologically normal course.

[Changes in arylsulphatase activity (EC 3.1.6.1) in the blood serum and urine in women during pregnancy and in the course of delivery]. / Zmiany aktywnosci arylosulfatazy A (EC 3.1.6.1) w surowicy i moczu kobiet w przebiegu ciazy i porodu fizjologicznego.

By the determination of arylsulphatase A activity (EC 3.1.6.1) in the blood serum and urine obtained from 66 women using the modified method by Lee-Vaupel and Conzelmann it was noticed the increase in the enzyme activity during the pregnancy comparing to the non-pregnant group. The highest enzyme activity was observed in the III trimester of pregnancy. In the following stages of delivery (I, II, III) it was assumed the increase in enzyme activity in urine. The highest enzyme activity in urine was observed in the stage III, and in the serum--in the stage II. It was compared the enzyme activity in primiparae and multiparae proving, that in the serum nd urine this activity is higher in the stages I and II in multiparae, and in the stage III in primiparae.

Development and disappearance of tolerance to induction of interferon and tumor necrosis factor response in athletes treated with natural immunostimulant.

The effect of nonspecific immunostimulation was examined in 15 basketball players subjected to extensive physical effort. The Tolpa* Torf Preparation (TTP*), a natural immunostimulating drug, was applied orally, one 5 mg tablet daily, in two 21-day cycles, separated by 2-week hiatus. Blood samples were collected 4 times, after each of two TTP* cycles and after the first and second hiatus. Whole blood assay was used to determine the spontaneous and induced production of interferon (IFN) and tumor necrosis factor (TNF). The levels of the cytokines were measured by microbioassays. TTP* stimulated synthesis of IFN and TNF in the whole blood cultures. However, after the oral administraton of TTP* for 3 weeks the leukocytes of the athletes developed hyporeactivity to IFN induction by TTP* and to a lesser extent to another "superinducer"--a mixture of phytohemagglutinin and bacterial lipopolysaccharide. The hyporeactivity state disappeared spontaneously within 2 weeks. In contrast, the tolerance to TNF induction did not develop during the TTP* administration. The increase of immunoglobulins, mainly of IgM and IgG classes and an acute phase protein--alpha1-antitrypsin, was observed at the late phase of the treatment. We suggest that the cytokine levels may be early markers for immunoprophylaxis. Furthermore, high production of IFN and TNF may be associated with extensive physical effort.

[Changes in arylsulphatase activity (EC 3.6.1) in the blood serum and urine in women during the pregnancy and in the course of delivery]. / Zmiany aktywnosci arylosulfatazy A (EC 3.1.6.1) w surowicy i moczu kobiet w przebiegu ciazy i porodu fizjologicznego.

By the determination of arylsulphatase A activity (EC 3.1.6.1) in the blood serum and urine obtained from 66 women using the modified method by Lee-Vaupel and Conzelmann it was noticed the increase in the enzyme activity during the pregnancy comparing to the non-pregnant group. The highest enzyme activity was observed in the III trimester of pregnancy. In the following stages of delivery (I, II, III) it was assumed the increase in enzyme activity in urine. The highest enzyme activity in urine was observed in the stage III, and in the serum--in the stage II. It was compared the enzyme activity in primiparae and multiparae proving, that in the serum nd urine this activity is higher in the stages I and II in multiparae, and in the stage III in primiparae.

[The arylsulphatase A (EC 3.1.6.1) activity in serum, urine and amniotic fluid from pregnant women with EPH-gestosis]. / Aktywnosc arylosulfatazy A (EC 3.1.6.1) w surowicy, moczu i plynie owodniowym kobiet ciezarnych z EPH-gestoza.

In serum, urine and amniotic fluid obtained from the 52. women divided into three groups the arylsulphatase A (EC 3.1.6.1) activity was measured by the modified Lee-Vaupel and Conzelmann method. It was noticed in serum from the pregnant women with EPH-gestosis the statistically significant (p < 0.05) increase in the enzyme activity comparing to the results from non-pregnant women and pregnant with normal course of pregnancy. It was no statistically significant differences in the urine from pregnant with EPH-gestosis and from the healthy pregnant, but there was the increase (p < 0.01) in the enzyme activity in amniotic fluid from pregnant women with EPH-gestosis comparing to the physiological course of pregnancy. According to our data, the arylsulphatase A activity assay could be recommended as a diagnostic marker in the EPH-gestosis.

[Gamma-glutamyltransferase (EC 2.3.2.2) activity in the course of brachytherapy of the uterine cervix and corpus carcinoma]. / Aktywnosc gamma-glutamylotransferazy (EC 2.3.2.2) w monitorowaniu brachyterapii raka szyjki i trzonu macicy.

The gamma-glutamyltransferase (EC 2.3.2.2) activity was measured in the blood serum obtained from 25 female patients with the uterine cervix carcinoma 10 and from 21 women with the uterine corpus carcinoma 10, treated by brachytherapy. The enzyme activity was determined in the five time points: before the brachytherapy, in the middle of the treating course, immediately after the application of the full dose of radium and in the seventh and the fourteenth day after the finishing the treatment. It was assumed the changes in the enzyme activity during the treatment course, and the highest values were observed immediately after the brachytherapy. It was noticed, that there is the direct connection between the enzyme activity changes and used brachytherapy method.

The concentration of cotinine in urine, colostrum and amniotic fluids within the system mother-baby.

The findings obtained by the authors of the thesis submit the new cognitive values to the diagnosis of pathology of pregnancy i.e. the influence of nicotine on the organisms of a mother and a new-born child, estimated by the assay of cotinine, the most important metabolite of nicotine. The authors lay a particular stress on the "colostrum-milk way" in the mother-child relationship and this is that needs to be emphasized in this thesis.

[Creatine kinase activity ec 2.7.3.2 in blood serum of women during pregnancy and during delivery]. / Aktywnosc kinazy kreatynowej ec 2.7.3.2 w surowicy krwi kobiet w przebiegu ciazy i okresach porodu.

In the serum of women during the pregnancy and during the stages of the delivery the activity of creatine kinase (CK) was measured using the test by Fermognost. The investigations were led in three 20-personal groups of women: non-pregnant, pregnant examined three times, in each trimester of pregnancy, and labored women examined three times, one time in each stage of delivery. The age of investigated women was between 18-39 years. According to obtained results, it was noticed statistically significant increase in the activity of this enzyme during the pregnancy. Measuring the activity of creatine kinase in three stages of delivery, the highest increase in this enzyme activity was observed in the third stage.

Average quantitative concentration of cotinine within the system pregnant woman-baby.

The findings presented by the authors of this report contribute to the diagnosis of pathology of pregnancy i.e. they assess the influence of nicotine on the organisms of a mother and a new-born child, estimated by concentration of cotinine, the most important metabolite of nicotine. The mean proportional share of cotinine in the fluids and organs in the pregnant women smoking actively was as follows: urine 72.1%, amniotic fluids 14.3%, colostrum 8.9% and placenta 4.7%. The authors pay a particular attention to the "colostrum-milk way" in the mother-child relationship what is emphasized in this report.

[The effect of exogenous iron on levels of adenosinetriphosphate and 2,3-diphosphoglycerate in erythrocytes of men during extreme physical exertion]. / Wplyw egzogennego zelaza na stezenie adenozynotrifosforanu I 2,3-difosfoglicerynianu w erytrocytach mezczyzn przy duzych obciazeniach fizycznych.

Nine men were examined during a three-week training requiring much physical effort. They were given nutrient, "LIVEX", enriched with iron. Hematological parameters as well as concentration of erythrocyte ATP and 2,3-DPG were determined before and after the experiment. Hematological parameters were determined using standard methods while Boehringer's test (Germany) was used for determining ATP and 2,3-DPG. The level of reticular cells was statistically higher after the experiment, and the increase in ATP and 2,3-DPG concentration was insignificant. A positive adaptation of energy metabolism after exogenous iron administration during physical effort was discussed.

Further studies on the etiopathology of malabsorption syndrome in broiler chickens.

The paper presents two cases of malabsorption syndrome in broiler chickens. The results indicate that the etiological factor appears to be the virus agent which causes necrosis in extra-excretional parts of the pancreas. During the course of the disease disorders in the enzymatic activity of gammaglutamyltransferase, leucylaminopeptidase and alanylaminopeptidase were observed. It consequently leads to the deterioration of intestines digestion and resorption of feed components. Application of Selcewet-Polfa to the diseased birds improved the health of the birds, morphological picture of peripheral blood and mineralisation of thigh bones. No radical improvement in the compensation of the final body weight was recorded.

The activity of alpha-amylase and gamma-amylase in serum and pancreatic homogenate of rats with experimental liver damage treated with colchicine.

In connection with extending the therapeutic application of colchicine to include its use in the treatment of liver fibrosis in cirrhosis and possible harmful effects of this drug on other organs, the pancreas in particular, the authors studied this problem experimentally. For this purpose a model of experimental cirrhosis in rats produced with chronic administration of carbon tetrachloride was used, and in the third and ninth months of the experiment the activity of alpha-amylase and gamma-amylase was determined in the serum and pancreatic homogenate. The results did not show any unfavourable effect of colchicine administered in their therapeutic doses for long time periods on the pancreas in experimental rats and in healthy controls.

Non-phosphorylative transglucosylation and other biochemical indices in serum and uterine myoma in women.

Non-phosphorylative transglucosylation, GGT and LAP activity, the level of some "acute phase" proteins i.e. sialic acids, ceruloplasmin, papain and trypsin inhibitors were examined in the serum, normal myometrium around tumours and in the uterine myoma of women. It was observed that the contents of proteins in the "acute phase" as well as GGT and LAP activity increased in the serum, whereas a decreased activity of these enzymes accompanied by increased transferase activity was observed in uterine myoma.

Protein: creatinine and trypsin inhibitor: creatinine ratios in the urine of marathon runners.

We measured changes in the protein: creatinine and trypsin inhibitor: creatinine ratios in the urine of six male marathon runners. Samples of urine were collected before the run, immediately after the run and in 6-h collections for 2 days. We found the greatest increase in the protein: creatinine ratio (2.6 times greater) in urine collected immediately after the run and the greatest increase in the trypsin inhibitor: creatinine ratio in urine samples collected 6-12 and 12-18 h after the run (2 and 3 times greater, respectively). This suggests the existence of different mechanisms for these two physiological processes. The later increase in the trypsin inhibitor: creatinine ratio was perhaps, due to a state of short-lived inflamation and shock after severe physical effort.

[The effect of marathon racing on serum concentrations of selected hormones in health and disabled individuals]. / Wplyw biegu maratonskiego na stezenie wybranych hormonów w surowicy krwi u osób zdrowych i u osób niepelnosprawnych.

Serum concentrations of the selected hormones were assayed in students who did not train long-distance races and in trained disabled individuals who passed the distance on a wheel chairs. Assays were carried out both prior to and after Marathon race. An exercise significantly increased serum concentrations of the following hormones in both groups: cortisol, ACTH, aldosteron, and plasma renin activity. An increase in total thyroxine, free thyroxine and decrease in LH were noted in disabled individuals. Increased concentrations of ACTH, free and total thyroxine, decreased concentrations of cortisol, LH, plasma renin activity, and aldosteron were found in disabled individuals who passed the distance of Marathon race on the wheel chairs comparing with those seen in the students.

In vitro studies into some parameters of protein and carbohydrate metabolism in lymphocytes infected with bovine leucosis virus.

Several parameters of protein and carbohydrate metabolism were determined in normal and leukemic lymphocytes in vitro in cattle, including arylamidase activity toward beta-naphthylamides of L-amino acids. The homogenate of bovine leukemic lymphocytes, in comparison with the control revealed increase of gamma-glutamyltransferase, activity trypsin inhibitor and papain inhibitor concentration and aldolase activity. On the other hand, proteolytic activity toward casein and histomucoid content decreased. Out of the 7 substrates used in the study, only 2, alanyl-beta-naphthylamide and leucyl-beta-naphthylamide, demonstrated lower activity in the leukemic material. Disorders in carbohydrate and protein metabolism in the observed lymphocytes in vitro in cattle are presented in the paper.