HPLC Method Validation for Jatropha dioica Extracts Analysis.

Jatropha dioica is a popular plant used in Mexican herbal medicine to treat several diseases. Cytotoxicity, antimicrobial and antiviral activities have been reported for root extracts, while riolozatrione, 6-epi-riolozatrione, citlalitrione and jatrophatrione, among others, have been identified as the principal components. In this work, an HPLC/DAD method for the analysis of riolozatrione and other major compounds in extracts of different polarities was validated. The analysis was carried out on an AccQ-Tag column with a water-acetonitrile mixture as mobile phase. Flow rate was 0.2 mL/min, and the separation was carried out in gradient mode with UV detection set at 254 nm. The resulting method showed good reproducibility in both retention times and peak areas of riolozatrione, 6-epi-riolozatrione, citlalitrione and jatrophatrione, with relative standard variations lower than 4.5 and 10.5% respectively. In addition, this method provides a good performance for riolozatrione quantitation, with recoveries between 102 and 108% and RSDs lower than 2.5%. The polarity of the extracting solvent did not affect the performance of the chromatographic method. The developed method was applied for the analysis and quantification of riolozatrione in extracts of Jatropha dioica collected in several seasonal stages and years (2014-2017).