[IMMUNOLOGICAL FACTORS OF THE INNATE IMMUNITY IN PATIENTS WITH HEPATOCELLULAR CARCINOMA OF A LARGE SIZE].

Factors of the inborn immunity in patients, suffering hepatocellular carcinoma of large size, were studied. Preoperatively the raising of metabolic activity of neutrophils in spontaneous NST-test, the neutrophils reserve reduction in NST-test, weak activation of the neutrophils absorption function in a phagocytosis reaction were noted. On the 7-8th postoperative day a reduction of quantity of formazan--positive neutrophils in spontaneous NST--test, raising of reserve of the neutrophils metabolic activity, comparing with initial values, were observed.

[Sensitivity of patients to immunomodulators in immunodeficiency diseases].

There were examined 198 patients, in whom immunodeficient (inflammatory, infectious and viral) diseases were revealed, as well as 10 healthy persons--for investigation of sensitivity of the blood neutrophils to immunomodulators (timalin, immunofan, polyoxidonium, timogen, erbisol). Most active one, in accordance to the investigation data, have evolved a synthetic preparation polyoxidonium, action mechanism of which is caused by direct activating impact on phagocytosis. Absence of sensitivity of the blood neutrophils towards activating stimuli (immunomodulators) in some patients was caused by a phenomenon, according to which the blood neutrophils, persisting durably in a preactivated state, further (while examining of the patients) have a reduced capacity for answering to stimulation. Such factors, as persisting in organism components of cellular wall of microorganisms or viruses, as well as antiinflammatory citokines, which are secreted in answer to the infectious agent introduction, are altogether promote the blood neutrophils preactivated state persistence. In patients, suffering viral infections, the blood neutrophils are mostly sensitive to immunofan and timalin. Immunologic preparations must be selected individually, taking into account the data of tests for sensitivity.

[Individual selection of immunocorrector in surgical treatment of obliterating atherosclerosis of the aorta and its branches].

The method for individual selection of immunocorrector in surgical treatment of patients, suffering obliterating atherosclerosis of abdominal aorta and its branches, was proposed. There were examined 69 patients, suffering affection of abdominal aorta and its branches. Inhibition of phagocytic function of neutrophils was observed; on background of activation of the oxygen-dependent metabolism; decompensation of the phagocytic cells function, not depending from the disease stage present. Introduction of the proposed method for the immunocorrector selection secures rising of the diagnosis accuracy and the immunotherapy efficacy.

[Peculiarities of immunity in patients, suffering gastric and duodenal ulcer disease].

The indices of local and the system immunity were studied up in the patients, suffering gastroduodenal ulcer disease. The local immunity peculiarities were noted, characterizing the lowering of the reparative processes activity and gastric ulcers refractivity to conservative treatment, thus confirming the necessity of active surgical tactics application in such patients.

[Non-specific factors of defense in patients with various forms of acute pancreatitis].

In patients, suffering an acute pancreatitis with purulent-septic complications, there are observed: the blood neutrophils metabolic activity raising in spontaneous NST--test, the reserve index of the blood neutrophils metabolic activity is secured, but the phagocytic number is lowered, raised or normal. In patients, who have died, the blood neutrophils metabolic activity in stimulated NST--test is raised, but preservation reserve of their metabolic activity is lowered, in comparison with such in a control group. Trustworthy raising of the blood neutrophils metabolic activity in stimulated NST--test was observed in patients, suffering infected pancreonecrosis, in comparison with such in aseptic necrosis, the phagocytic number in them was trustworthy reduced. In patients, suffering an acute pancreatitis, who have died, there was established the reversed correlation dependence between the formazanpositive blood neutrophils quantity in spontaneous NST--test and preservation reserve.

[Application of ozone therapy in complex treatment of patients with acute necrotizing pancreatitis].

Examination and treatment of 32 patients was done, in whom an acute necrotic pancreatitis was diagnosed. In 16 patients (the main group) ozonotherapy, according to scheme, elaborated in the clinic, was added to conventional treatment. The ozonotherapy application had permitted to reduce the rate of performance of "open" operative interventions. The cellular, humoral and local immunity improvement and biochemical indices normalization were noted. The stationary treatment duration was 21.4 days at average in the main group and 34.5 -- in a control one. The relaparotomy performance rate in the main group was lesser than in a control one.

[Effect of cerulloplasmin on natural factors of defense in patients with an acute necrotic pancreatitis].

In 81.25% patients with an acute necrotic pancreatitis and in 50% of healthy persons the blood neutrophils are sensitive to cerulloplasmin in metabolic activity. In 62.5% of patients the phagocytic index have had reduced in addition of cerulloplasmin. Phagocytic number in addition of cerulloplasmin have constituted over 1,5 conditional units, in 75% of patients, without such addition - in 84%. The conclusion was made about necessity of individual selection of immunomodulators in patients with an acute necrotic pancreatitis.

[Main principles of antibiotic prophylaxis and antibacterial therapy in abdominal surgery].

The principles of antibioticoprophylaxis and antibacterial therapy in abdominal surgery were expounded. There are adduced the most frequently revealed pathogenic microorganisms, inducing intraabdominal infection, the ways of lowering of the antibacterial therapy expenses, new approaches to application of immunocorrection and directed transport of antibiotics in surgery as well as empirical and etiotropic treatment, basing on the taking account of the spread and antibioticosensitivity of pathogenic microorganisms of nosocomial infections. There was shown, that concentration of interleucin-1beta and tumor necrosis factor-alpha in the blood serum is connected with the severity of patient state. Significant rising of this cytokines concentration constitutes the trustworthy criterion of the ineffective therapy and mortality risk. The influence of microorganisms and some antibiotics on induction of interferons was studied. Amoxicyllin, polymixin, vancomycin, cephalosporins and fluconazol are the most active inductors of gamma-interferon. Maximal efficacy of antibacterial therapy is possible only if an adequate surgical intervention was performed.

[The application efficacy of immunomodulators in complex of treatment of an acute necrotic pancreatitis]. / Efektyvnist' zastosuvannia imunomoduliatoriv v kompleksi likuvannia hostroho nekrotychnoho pankreatytu.

Analysis of efficacy of the immunomodulators application, thymalin in particular, in complex treatment algorhythm in 18 patients with an acute necrotic pancreatitis was conducted. Efficacy of immunocorrecting therapy in the immune state improvement, purulent-septic complications prophylaxis, the abortive course of the disease promotion was established In 12 (66.7) patients the disease regression was noted, in 2 (11.1%)--the inflammatory process delineation with pancreatic pseudocyst formation was observed, 4 (22.2%) patients were operated on. All the patients survived.

[Dynamics in the local and systemic immune factors in patients with cystic transformation of the bile duct]. / Dinamika mestnykh i sistemnykh faktorov zashchity u bol'nykh s kistoznoi transformatsiei zhelchnykh protokov.

Cystic transformation of biliary ducts (CTBD) is a rare inborn anomaly of the biliary system development. For the patients with CTBD quite high metabolic activity of neutrophils according to data of spontaneous test with nitroblue tetrazolium (NBT-test), reduction of IgA content in the initial period, significant raising of interleukin-6 (IL-6) level on the first postoperative day are characteristic. Quantity of active neutrophils of type II in the blood of patients with CTBD depends on antigenic load. Neutrophils in the bile restore the NBT according to type I. Local concentration of IL-6 and IL-8, and also the functional activity of neutrophils in ductal bile in patients with CTBD are the sensitive indexes of clinical state and immunological reactivity.

[The application of laferon in a combined therapy of purulent cholangitis]. / Primenenie laferona v komplekse terapii gnoinogo kholangita.

Results of treatment of 160 patients, operated on for nontumoral biliary ducts impassibility, complicated by purulent cholangitis, were analyzed. In 60 patients intraoperatively and after the operation in complex of treatment laferon solution was injected into biliary ducts in dosage 500,000--1 million IU a day, permitting to reduce the frequency of the postoperative complications occurrence from 62.2 to 36% and of postoperative mortality--from 13 to 6%. Regional immune therapy using laferon stimulates the factors of organism inflammatory reaction and hepatocytes functional state restoration.

[Interleukin-6 content in the blood serum and bile duct in patients with obturative jaundice of different origin]. / Soderzhanie interleikin-6 v syvorotke krovi i protokovoi zheltukhoi razlichnogo geneza.

There were examined 19 patients with nontumoral disease and 13--with hepatopancreatoduodenal zone organs tumor. In nontumoral disease interleukin-6 (IL-6) was revealed in 83.3% of patients in the blood serum and in 88.9%--in ductal bile; in nontumoral impassability of biliary ducts IL-6 was revealed in the blood serum in 53.8% of patients, in ductal bile--in 87.5%. It was established, that bilirubinemia degree did not correlate with IL-6 content in the blood serum, but was directly influenced by its concentration in ductal bile.

[The factors of cell immunity in patients with peptic ulcer of duodenum]. / Faktory klitynnoho imunitetu u patsiientiv z vyrazkovoiu khvoroboiu dvanadtsiatypaloï kyshky.

20 patients with peptic ulcer disease of duodenum were studied. We found that metabolic activity of neutrophils in peripheral blood and in mucosa is the same in patients with complicated peptic ulcer. The absorption activity of tissue neutrophils is higher than in blood. During operation we observed an increase of neutrophils' activity both in blood and mucosa. On the 6th day after operation the response of neutrophils recovered. In patients who undergo conservative treatment for peptic ulcer of duodenum the activity of neutrophils decreased significantly. This emphasizes that immunocorrection treatment is indicated to all patients that receive conservative treatment for peptic ulcer.

[The meaning of interleukin-6 in chronic biliary pancreatitis]. / Znachenie interleikina-6 pri khronicheskom biliarnom pankreatite.

The interleukin-6 (IL-6) contents in the blood serum and in bile from the gallbladder was studied in 25 patients with uncomplicated cholelithic disease and with that, complicated by chronic biliary pancreatitis. Laparoscopic cholecystectomy was done in all the patients. In uncomplicated cholelithic disease IL-6 was not revealed in the blood serum and in majority of observations--in bile from gallbladder. Direct dependence was established between duration of cholelithic disease existence, complicated by chronic biliary pancreatitis, and concentration of IL-6 in bile from gallbladder.

[The characteristics of the local protective factors in disseminated suppurative peritonitis]. / Osobennosti faktorov mestnoi zashchity pri rasprostranennom gnoinom peritonite.

A comparative evaluation was done of factors of the local and systemic immunity in 18 patients with diffuse pyoperitonitis. Morphological (neutrophiles from the abdominal cavity in patients with peritonitis retrieve NBT in a diffuse-dotted fashion whereas neutrophiles of blood give a diffuse pattern and by granules) and functional (reduced metabolic activity of abdominal neutrophiles in a spontaneous NBT-test and an augmented index for the reserve versus metabolic activity of blood neutrophiles) differences in local and systemic factors of immune defence were noted. It is shown that the content of all classes of immunoglobulins is significantly lower in the peritoneal exudate than it is in blood serum. The above facts are to be considered in treatment of patients with generalized purulent peritonitis.

[The influence of bile on the survival of leukocyte in patients with purulent cholangitis]. / Vliianie zhelchi na vyzhivaemost' leikotsitov u bol'nykh s gnoinym kholangitom.

It was established that while incubation of the blood leucocytes of a healthy person with duodenal bile of a patient, suffering chronical purulent cholangitis, the leucocytes life span enhances. In a patient's bile the leucocytes survival is higher than in healthy person's.

[The specific aspects of humoral and local cell immunity in patients with purulent cholangitis]. / Osobennosti gumoral'nykh i kletochnykh faktorov mestnogo immuniteta u bol'nykh gnoinym kholangitom.

The existence of autonomous system of the biliary ducts local immunity was established. The contents of the antibodies and C3-component complement in intraductal bile in patients with purulent cholangitis (PC) is normalizing during an early postoperative period. In a common biliary duct bile while PC a polymorphonuclear neutrophils are regularly revealed, which restore nitroblue tetrazolium (NBT) diffusely. The NBT test application in PC diagnosis is substantiated.

[Immunomodulator sensitivity in patients with peritonitis]. / Chuvstvitel'nost' bol'nykh s peritonitom k immunomoduliatoram.

The indexes of the loading rosetteforming tests were studied up in vitro in 10 healthy persons and in 12 patients with peritonitis. In the patients the hyporeactive type of reactivity and multidirectional reaction on immunomodulators was revealed more frequently by comparison with such in a healthy persons. The individual option of the immunocorrecting agents is mandatory in patients with an acute peritonitis.

[State of cellular and humoral immunity after soft tissue endoprosthesis implantation and correction of body forms using biogel Interfall]. / Sostoianie kletochnonogo i gumoral'nogo immuniteta posle éndoprotezirovaniia miagkikh tkanei i korrektsii form tela s pomoshch'iu biogelia "Interfall".

Injection implantation of polyacrylamide gel "Interfall" in soft tissues was conducted in 110 patients in 1992-1997 yrs. In 17 patients the mammary glands prosthesis was conducted using biogel. Biogel did not influence the cell subpopulations of the immunity T- and B-systems, the immunoglobulins level and did not cause the organism sensibilization.