RESUMEN
AIM: To study a neuroprotective effect of mexidol on the cell model of glutamate stress. MATERIAL AND METHODS: Cytological studies of an effect of glutamate stress on cerebellar granule cells were carried out. RESULTS: Mexidol increased neuronal survival after the addition of glutamate by 8-10% (p<0.05). The effect of mexidol was more pronounced at the stage of neuron culture growth (5 days), cell survivability increased on average by 20%. CONCLUSION: The results of the study confirmed the neuroprotective effect of mexidol in the neuronal culture in glutamate toxicity model.
Asunto(s)
Antioxidantes/farmacología , Neuronas/efectos de los fármacos , Fármacos Neuroprotectores/farmacología , Picolinas/farmacología , Animales , Supervivencia Celular , Células Cultivadas , Cerebelo/citología , Cerebelo/efectos de los fármacos , Ácido Glutámico/toxicidad , Modelos Biológicos , RatasRESUMEN
OBJECTIVE: To study the synergism between neuropeptides and lithium ions. MATERIAL AND METHODS: An experimental model of stroke (chronic bilateral occlusion of the common carotid arteries in rats), neuronal culture studies, histomorphological analyses, determination of micronutrient profile of brain substrates were used. RESULTS: A complex of experimental studies revealed that the effect of cerebrolysin is influenced by the synergism between lithium ions and the neuropeptide contentof this drug. Pharmacokinetic synergism promotes the accumulation of lithium in brain tissues during cerebrolysin treatment. The existence of the pharmacokinetic synergism is evident from the potentiation of neuroprotective effects of the drug under the action of lithium ions established in the model of stroke. CONCLUSION: Lithium ions potentiate neuroprotective effects of cerebrolysin.
Asunto(s)
Aminoácidos/farmacocinética , Encefalinas/farmacocinética , Galanina/farmacocinética , Péptidos y Proteínas de Señalización Intracelular/farmacocinética , Compuestos de Litio/farmacocinética , Neuropéptidos/farmacocinética , Fármacos Neuroprotectores/farmacocinética , Aminoácidos/administración & dosificación , Animales , Encéfalo/efectos de los fármacos , Encéfalo/metabolismo , Modelos Animales de Enfermedad , Sinergismo Farmacológico , Encefalinas/administración & dosificación , Galanina/administración & dosificación , Ácido Glutámico/toxicidad , Péptidos y Proteínas de Señalización Intracelular/administración & dosificación , Compuestos de Litio/administración & dosificación , Masculino , Neuropéptidos/administración & dosificación , Fármacos Neuroprotectores/administración & dosificación , Orexinas , Ratas , Ratas Endogámicas , Accidente Cerebrovascular/tratamiento farmacológico , Accidente Cerebrovascular/patologíaRESUMEN
It was stated with model of bilateral photochemically induced thrombosis of the prefrontal cortex by injected intranasally or intraperitoneally in 1h after operation new derivatives of eritropoetine: Epo, Epo-Fc, Epo-Tr provoked neuroprotective and antiamnestic action. Epo-Fc demonstrated more effective action by intranasal injection.
Asunto(s)
Eritropoyetina/farmacología , Trombosis Intracraneal/tratamiento farmacológico , Fármacos Neuroprotectores/farmacología , Corteza Prefrontal/metabolismo , Animales , Trombosis Intracraneal/metabolismo , Trombosis Intracraneal/patología , Masculino , Corteza Prefrontal/patología , RatasRESUMEN
The neuroprotective effects of dipeptide GK-2h, a mimetic of nerve growth factor, in bifocal photoinduced ischemia in rat brain prefrontal cortex was studied. It was shown that GK-2h, injected intraperitonealy in dose 0.1 mg/kg in 1 h or 4 h after operation and then on 2nd, 4th and 8th days, prevented significantly on 9th day from increasing volume of cortical infarction.
Asunto(s)
Isquemia Encefálica/tratamiento farmacológico , Fármacos Neuroprotectores/farmacología , Péptidos/farmacología , Corteza Prefrontal/efectos de los fármacos , Animales , Isquemia Encefálica/patología , Masculino , Factores de Crecimiento Nervioso/química , Fármacos Neuroprotectores/química , Fármacos Neuroprotectores/uso terapéutico , Péptidos/química , Péptidos/uso terapéutico , Corteza Prefrontal/patología , RatasRESUMEN
The neuroprotective and antiamnestic effects of GK-2 dipeptide (nerve growth factor mimetic) were studied on rats with photoinduced bilateral focal ischemia of the prefrontal cortex. Intraperitoneal injection of GK-2 in a dose of 1 mg/kg on days 1, 2, 4, and 6 postoperation led to a 62% reduction of cortical infarction volume on day 9 and completely preserved conditioned passive avoidance response trained before stroke.