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1.
Sci Rep ; 13(1): 14269, 2023 08 31.
Artículo en Inglés | MEDLINE | ID: mdl-37652935

RESUMEN

Water and nitrogen (N) are the most limiting factors to plant productivity globally, but we lack a critical understanding of how water availability impacts N dynamics in agricultural systems. Plant N requirements are particularly uncertain when water is limited because of the interactive effect of water and N on plant growth, N demand, and plant uptake. We investigated impacts of N application and water availability on plant growth and N movement, including above and belowground growth, water productivity, N productivity, N uptake, N recovery, and greenhouse gas emissions within a semi-arid system in northeastern Colorado, USA. Moderately high soil N availability depressed grain yield and shoot growth under both limited and full water availability, despite no indication of physical toxicity, and came with additional risk of deleterious N losses. Under low N availability, plant N concentrations in aboveground tissues showed greater recovery of N than what was applied in the low N treatments under both full and limited water availability. This enhanced recovery underscores the need to better understand both plant soil foraging and processes governing resource availability under these conditions. Finally, limited water availability reduced N uptake across all N treatments and left 30% more soil nitrate (NO3-) deep in the soil profile at the end of the season than under full water availability. Our results show that plant N needs are not linearly related to water use and emphasize the need for an integrated understanding of water and N interactions, plant foraging for these resources, and the dynamics of processes that make N available to plants.


Asunto(s)
Agricultura , Zea mays , Transporte Biológico , Suelo , Agua
2.
J Adolesc ; 95(2): 382-396, 2023 02.
Artículo en Inglés | MEDLINE | ID: mdl-36372961

RESUMEN

INTRODUCTION: This study investigated a theoretically derived peer conversation style, problem anxiety talk, to examine the emotional regulation of anxiety in dyads as a possible mechanism of anxiety contagion that occurs between adolescent friends. METHODS: In Study 1, 376 late adolescents residing in the United States, ages 17-24 with 68% female, responded to measures of anxiety, worry, friendship quality, depression, co-rumination, and a new measure of problem anxiety talk. In Study 2 problem anxiety talk, stress, anxiety and depression were evaluated in 481 late adolescents, ages 17-24 with 64% female. RESULTS: Once controlling for worry and co-rumination, problem anxiety talk with a close friend predicted anxiety, stress and depression for both samples of late adolescents. Factor analysis in each sample supported problem anxiety talk as a single factor with high internal consistency. CONCLUSIONS: Findings indicate that problem anxiety talk is a distinct construct representing interpersonal disclosures between friends involving threat perception, likelihood of negative events, and lack of coping skills; results also indicate that problem anxiety talk is associated with anxiety, stress, worry, and depression. Implications of problem anxiety talk in its potential role in the etiology and spread of anxiety are discussed.


Asunto(s)
Amigos , Relaciones Interpersonales , Humanos , Adolescente , Femenino , Adulto Joven , Adulto , Masculino , Amigos/psicología , Encuestas y Cuestionarios , Ansiedad/psicología , Grupo Paritario
3.
J Environ Qual ; 51(5): 877-889, 2022 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-35436352

RESUMEN

Precise water and fertilizer application can increase crop water productivity and reduce agricultural contributions to greenhouse gas (GHG) emissions. Regulated deficit irrigation (DI) and drip fertigation control the amount, location, and timing of water and nutrient application. Yet, few studies have measured GHG emissions under these practices, especially for maize (Zea mays L.). The objective was to quantify N2 O and CO2 emission from DI and full irrigation (FI) within a drip-fertigated maize system in northeastern Colorado. During two growing seasons of measurement, treatments consisted of mild, moderate, and extreme DI and FI. Deficit irrigation was managed based on growth stage so that full evapotranspiration (ET) was met during the yield-sensitive reproductive stage, but less than full crop ET was applied during the late vegetative and maturation growth stages. In the first year, mild DI (90% ET) reduced N2 O emissions by 50% compared with FI. In the second year, compared with FI, moderate DI (69-80% ET) reduced N2 O emissions by 15%, and extreme DI (54-68% ET) reduced N2 O emissions by 40%. Only extreme DI in the second year significantly reduced CO2 emissions (by 30%) compared with FI. Mild DI reduced yield-scaled emissions in the first year, but moderate and extreme DI had similar yield-scaled emissions as FI in the second year. The surface drip fertigation resulted in total GHG emissions that were one-tenth of literature-based measurements from sprinkler-irrigated maize systems. This study illustrates the potential of DI and drip fertigation to reduce N2 O and CO2 emissions in irrigated cropping systems.


Asunto(s)
Gases de Efecto Invernadero , Riego Agrícola/métodos , Agricultura/métodos , Dióxido de Carbono/análisis , China , Colorado , Fertilizantes/análisis , Gases de Efecto Invernadero/análisis , Óxido Nitroso/análisis , Suelo , Agua , Zea mays
4.
J Sch Nurs ; 38(3): 249-258, 2022 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-32410495

RESUMEN

This study examined the association between two implementation factors, nurse-reported intervention adherence and self-efficacy, and children's outcomes in school nurse-delivered anxiety interventions. Data were collected in a pilot randomized controlled effectiveness trial with 54 children and 21 school nurses. Nurses implemented either a cognitive behavioral or relaxation-skills-only intervention. Nurse questionnaires assessed implementation factors. Independent evaluators assessed changes in children's anxiety symptoms at postintervention and at 3-month follow-up using clinical improvement and global functioning scales. Regression analyses indicated that greater intervention adherence was associated with greater anxiety symptom improvement at follow-up. Nurse self-efficacy interacted with intervention group, such that nurses with higher self-efficacy who implemented the cognitive behavioral intervention tended to have children show improvement and higher postintervention functioning. The impact of implementation factors on children's outcomes may differ depending on intervention type. Self-efficacy may be important for nurses using relatively complex interventions. Intervention adherence should be supported through training and consultation.


Asunto(s)
Ansiedad , Autoeficacia , Ansiedad/terapia , Trastornos de Ansiedad , Niño , Humanos
5.
Sci Total Environ ; 793: 148569, 2021 Nov 01.
Artículo en Inglés | MEDLINE | ID: mdl-34328984

RESUMEN

Soil organic carbon (SOC), as the largest terrestrial carbon pool, plays an important role in global carbon (C) cycling, which may be significantly impacted by global changes such as nitrogen (N) fertilization, elevated carbon dioxide (CO2), warming, and increased precipitation. Yet, our ability to accurately detect and predict the impact of these global changes on SOC dynamics is still limited. Investigating SOC responses to global changes separately for mineral-associated organic carbon (MAOC) and the particulate organic carbon (POC) can aid in the understanding of overall SOC responses, because these are formed, protected, and lost through different pathways. To this end, we performed a systematic meta-analysis of the response of SOC, MAOC, and POC to global changes. POC was particularly responsive, confirming that it is a better diagnostic indicator of soil C changes in the short-term, compared to bulk SOC and MAOC. The effects of elevated CO2 and warming were subtle and evident only in the POC fraction (+5.11% and - 10.05%, respectively), while increased precipitation had no effects at all. Nitrogen fertilization, which comprised the majority of the dataset, increased SOC (+5.64%), MAOC (+4.49%), and POC (+13.17%). Effect size consistently varied with soil depth and experiment length, highlighting the importance of long-term experiments that sample the full soil profile in global change SOC studies. In addition, SOC pool responses to warming were modified by degree of warming, differently for air and soil warming manipulations. Overall, we suggest that MAOC and POC respond differently to global changes and moderators because of the different formation and loss processes that control these pools. Coupled with additional plant and microbial measurements, studying the individual responses of POC and MAOC improves understanding of the underlying dynamics of SOC responses to global change. This will help inform the role of SOC in mitigating the climate crisis.


Asunto(s)
Carbono , Suelo , Secuestro de Carbono , Minerales , Material Particulado
6.
J Clin Child Adolesc Psychol ; 50(2): 177-186, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-31373524

RESUMEN

The goal of this study was to evaluate the feasibility and impact of brief school-nurse-administered interventions for reducing anxiety. Thirty school nurses in Connecticut and Maryland were randomly assigned to deliver the Child Anxiety Learning Modules (CALM; n = 14) or CALM-Relaxation only (CALM-R; n = 16). Students (N = 54) were ages 5-12 (M age = 8; 84.9% White; 68.5% female) with elevated anxiety symptoms and/or anxiety disorders. Feasibility was assessed based on recruitment, retention, attendance, training and intervention satisfaction, and intervention adherence. Multiple informants, including independent evaluators (IEs), completed measures of clinical improvement at postintervention and at a 3-month follow-up. Of nurses in CALM and CALM-R, 62% and 81%, respectively, enrolled a student and completed an average of 6 sessions. Youth retention was 85% and 94% in CALM and CALM-R, respectively. Training and intervention satisfaction were high. At postintervention and follow-up, youth in both groups showed significant reductions in anxiety and related symptoms and improvements in functioning. Within-group effect sizes were medium to large, and between-group effect sizes were small. Task shifting responsibility for delivering brief mental health interventions to school nurses is feasible and shows promise for reducing anxiety and related impairment. This approach may also be integrated within a response to intervention model used in schools.Public Health Significance: Brief school-nurse-administered anxiety reduction interventions were shown to be feasible and had a positive impact on student anxiety and related impairment highlighting that school nurses can be an important school resource.


Asunto(s)
Trastornos de Ansiedad/terapia , Ansiedad/terapia , Enfermeras y Enfermeros , Servicios de Salud Mental Escolar , Instituciones Académicas , Estudiantes/psicología , Ansiedad/prevención & control , Trastornos de Ansiedad/prevención & control , Niño , Preescolar , Femenino , Humanos , Masculino , Proyectos Piloto
7.
Sci Adv ; 5(12): eaav9318, 2019 12.
Artículo en Inglés | MEDLINE | ID: mdl-31897423

RESUMEN

Bio-based energy is key to developing a globally sustainable low-carbon economy. Lignocellulosic feedstock production on marginally productive croplands is expected to provide substantial climate mitigation benefits, but long-term field research comparing greenhouse gas (GHG) outcomes during the production of annual versus perennial crop-based feedstocks is lacking. Here, we show that long-term (16 years) switchgrass (Panicum virgatum L.) systems mitigate GHG emissions during the feedstock production phase compared to GHG-neutral continuous corn (Zea mays L.) under conservation management on marginally productive cropland. Increased soil organic carbon was the major GHG sink in all feedstock systems, but net agronomic GHG outcomes hinged on soil nitrous oxide emissions controlled by nitrogen (N) fertilizer rate. This long-term field study is the first to demonstrate that annual crop and perennial grass systems respectively maintain or mitigate atmospheric GHG contributions during the agronomic phase of bioenergy production, providing flexibility for land-use decisions on marginally productive croplands.

8.
J AAPOS ; 21(5): 402-406, 2017 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-28890077

RESUMEN

Occlusion therapy remains the mainstay treatment of amblyopia, but its outcome is not assured or universally excellent. Many factors are known to influence treatment outcome, among which compliance is foremost. The occlusion dose monitor (ODM) removes one variable from the treatment equation, because it records the occlusion actually received by-rather than prescribed for-the child. Improvement observed can thus be quantitatively related to the patching received. This review summarizes the insights the ODM has provided to date particularly in elucidating the dose-response relationship. We are entering the era of personalized ophthalmology in which treatments will be tailored to the needs of the individual child and facilitated by the use of wearable monitors.


Asunto(s)
Ambliopía/terapia , Vendajes , Monitoreo Fisiológico , Privación Sensorial , Humanos , Cooperación del Paciente
9.
Glob Chang Biol ; 23(7): 2848-2862, 2017 07.
Artículo en Inglés | MEDLINE | ID: mdl-28135027

RESUMEN

Over the last 50 years, the most increase in cultivated land area globally has been due to a doubling of irrigated land. Long-term agronomic management impacts on soil organic carbon (SOC) stocks, soil greenhouse gas (GHG) emissions, and global warming potential (GWP) in irrigated systems, however, remain relatively unknown. Here, residue and tillage management effects were quantified by measuring soil nitrous oxide (N2 O) and methane (CH4 ) fluxes and SOC changes (ΔSOC) at a long-term, irrigated continuous corn (Zea mays L.) system in eastern Nebraska, United States. Management treatments began in 2002, and measured treatments included no or high stover removal (0 or 6.8 Mg DM ha-1  yr-1 , respectively) under no-till (NT) or conventional disk tillage (CT) with full irrigation (n = 4). Soil N2 O and CH4 fluxes were measured for five crop-years (2011-2015), and ΔSOC was determined on an equivalent mass basis to ~30 cm soil depth. Both area- and yield-scaled soil N2 O emissions were greater with stover retention compared to removal and for CT compared to NT, with no interaction between stover and tillage practices. Methane comprised <1% of total emissions, with NT being CH4 neutral and CT a CH4 source. Surface SOC decreased with stover removal and with CT after 14 years of management. When ΔSOC, soil GHG emissions, and agronomic energy usage were used to calculate system GWP, all management systems were net GHG sources. Conservation practices (NT, stover retention) each decreased system GWP compared to conventional practices (CT, stover removal), but pairing conservation practices conferred no additional mitigation benefit. Although cropping system, management equipment/timing/history, soil type, location, weather, and the depth to which ΔSOC is measured affect the GWP outcomes of irrigated systems at large, this long-term irrigated study provides valuable empirical evidence of how management decisions can impact soil GHG emissions and surface SOC stocks.


Asunto(s)
Productos Agrícolas , Calentamiento Global , Zea mays/crecimiento & desarrollo , Riego Agrícola , Agricultura , Efecto Invernadero , Óxido Nitroso , Suelo
10.
Strabismus ; 24(4): 161-168, 2016 12.
Artículo en Inglés | MEDLINE | ID: mdl-27929726

RESUMEN

PURPOSE: To generate a statistical model for personalizing a patient's occlusion therapy regimen. METHODS: Statistical modelling was undertaken on a combined data set of the Monitored Occlusion Treatment of Amblyopia Study (MOTAS) and the Randomized Occlusion Treatment of Amblyopia Study (ROTAS). This exercise permits the calculation of future patients' total effective dose (TED)-that predicted to achieve their best attainable visual acuity. Daily patching regimens (hours/day) can be calculated from the TED. RESULTS: Occlusion data for 149 study participants with amblyopia (anisometropic in 50, strabismic in 43, and mixed in 56) were analyzed. Median time to best observed visual acuity was 63 days (25% and 75% quartiles; 28 and 91 days). Median visual acuity in the amblyopic eye at start of occlusion was 0.40 logMAR (quartiles 0.22 and 0.68 logMAR) and at end of occlusion was 0.12 (quartiles 0.025 and 0.32 logMAR). Median lower and upper estimates of TED were 120 hours (quartiles 34 and 242 hours), and 176 hours (quartiles 84 and 316 hours). The data suggest a piecewise linear relationship (P = 0.008) between patching dose-rate (hours/day) and TED with a single breakpoint estimated at 2.16 (standard error 0.51) hours/day, suggesting doses below 2.16 hours/day are less effective. CONCLUSION: We introduce the concept of TED of occlusion. Predictors for TED are visual acuity deficit, amblyopia type, and age at start of occlusion therapy. Dose-rates prescribed within the model range from 2.5 to 12 hours/day and can be revised dynamically throughout treatment in response to recorded patient compliance: a personalized dosing strategy.


Asunto(s)
Ambliopía/terapia , Vendajes , Modelos Estadísticos , Medicina de Precisión , Privación Sensorial , Ambliopía/fisiopatología , Femenino , Humanos , Lactante , Masculino , Cooperación del Paciente , Factores de Tiempo , Resultado del Tratamiento , Agudeza Visual/fisiología
11.
Strabismus ; 24(3): 109-12, 2016 09.
Artículo en Inglés | MEDLINE | ID: mdl-27485937

RESUMEN

AIMS: The proportion of patients seen by the paediatric eye service that attend for reasons related to amblyopia has not been quantified. The purpose of this study was to quantify the proportion of patients seen in the paediatric eye service attending for reasons related to amblyopia. METHODS: Records of all eye appointments of children attending the Hillingdon Hospitals NHS Foundation Trust and St Mary's Hospital Imperial College Healthcare NHS Trust over one month in 2009 were examined to determine the diagnosis and reason for attendance. RESULTS: Seven hundred and four patients had appointments booked at St Mary's and Hillingdon in March 2009. The fail-to-attend rates were not significantly different at the 2 sites (19% at St Mary's and 9% at Hillingdon; P=0.75). Of the 704 patients, 533 (St Mary's, 252 [75%]; Hillingdon, 281 [76%]) were attending for amblyopia-related reasons. Of the overall 982 booked appointments, 770 (79%) were amblyopia-related. CONCLUSIONS: Amblyopia diagnosis and management is clearly the most common cause of attendance to the paediatric eye service, accounting for over three-quarters of outpatient visits.


Asunto(s)
Ambliopía/diagnóstico , Ambliopía/terapia , Servicios de Salud del Niño/estadística & datos numéricos , Hospitales Pediátricos/estadística & datos numéricos , Pautas de la Práctica en Medicina/estadística & datos numéricos , Carga de Trabajo/estadística & datos numéricos , Adolescente , Niño , Preescolar , Femenino , Humanos , Lactante , Masculino , Estudios Prospectivos , Adulto Joven
12.
J Environ Qual ; 45(3): 906-14, 2016 May.
Artículo en Inglés | MEDLINE | ID: mdl-27136157

RESUMEN

Dairy manure is often applied to cropped soils as a substitute for inorganic N fertilizers, but the impacts of manure on soil trace gas fluxes, yields, and soil N are uncertain in the semiarid western United States. Soil carbon dioxide (CO-C), methane (CH-C), nitrous oxide (NO-N), and ammonia (NH-N) emissions were monitored using surface chambers from five N treatments: (i) partially composted solid dairy manure (DM) (412 kg N ha), (ii) DM + AgrotainPlus (DM+AP), (iii) enhanced efficiency N fertilizer (SuperU [SU]) (179 kg N ha), (iv) urea (179 kg N ha), and (v) check (no N applied), to determine their effect on growing season (GS) and nongrowing season emissions from a tilled clay loam soil under irrigated, continuous corn production for 3 yr. SuperU and AgrotainPlus contain urease and nitrification inhibitors. Averaged over years, GS soil CO-C emissions were greater for DM and DM+AP than for urea, SU, and check treatments due to the large amount of C added with the manure; CH-C emissions did not vary among N treatments; and NO-N emissions decreased in the order urea = DM = DM+AP > SU > check. AgrotainPlus added to the DM did not reduce NO-N emissions compared with DM. Cumulative NH-N emissions after manure application decreased in the order urea > SU > check, with no significant differences between SU, DM, and DM+AP. Dairy manure provided slow-release N with nitrate intensities lower than urea and NO-N emissions similar to urea. These results highlight the importance of best-management practices such as immediate irrigation after N application and use of urease and nitrification inhibitors to minimize N losses.


Asunto(s)
Estiércol , Nitrógeno , Zea mays , Fertilizantes , Nitratos/análisis , Óxido Nitroso , Suelo
13.
J Child Adolesc Psychiatr Nurs ; 28(3): 121-30, 2015 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-26171792

RESUMEN

PROBLEM: Excessive anxiety is among the most common psychiatric problems facing youth. Because anxious youth tend to have somatic complaints, many seek help from the school nurse. Thus, school nurses are in an ideal position to provide early intervention. This study addresses this problem and describes the plans to develop and test a new intervention (Child Anxiety Learning Modules; CALM), delivered by school nurses, to reduce child anxiety and improve academic functioning. METHODS: An iterative development process including consultation with an expert panel, two open trials, and a pilot randomized controlled study comparing CALM to usual care is proposed. Feedback will be solicited from all participants during each phase and data on outcome measures will be provided by children, parents, teachers, and independent evaluators. FINDINGS: Data will be collected on intervention satisfaction and feasibility. Primary outcomes that include child anxiety symptoms, classroom behavior, and school performance (e.g., attendance, grades, standardized test scores) will be collected at pre- and post-interventions and at a 3-month follow-up evaluation. CONCLUSIONS: Pediatric anxiety is a common problem that school nurses frequently encounter. Consequently, they are well positioned to play a key role in enhancing access to behavioral health interventions to reduce anxiety and may therefore make a significant positive public health impact.


Asunto(s)
Trastornos de Ansiedad/terapia , Ansiedad/terapia , Evaluación de Procesos y Resultados en Atención de Salud , Psicoterapia/métodos , Servicios de Enfermería Escolar/métodos , Niño , Estudios de Factibilidad , Humanos , Proyectos Piloto
14.
Trials ; 16: 189, 2015 Apr 25.
Artículo en Inglés | MEDLINE | ID: mdl-25906974

RESUMEN

BACKGROUND: Amblyopia is the commonest visual disorder of childhood in Western societies, affecting, predominantly, spatial visual function. Treatment typically requires a period of refractive correction ('optical treatment') followed by occlusion: covering the nonamblyopic eye with a fabric patch for varying daily durations. Recent studies have provided insight into the optimal amount of patching ('dose'), leading to the adoption of standardized dosing strategies, which, though an advance on previous ad-hoc regimens, take little account of individual patient characteristics. This trial compares the effectiveness of a standardized dosing strategy (that is, a fixed daily occlusion dose based on disease severity) with a personalized dosing strategy (derived from known treatment dose-response functions), in which an initially prescribed occlusion dose is modulated, in a systematic manner, dependent on treatment compliance. METHODS/DESIGN: A total of 120 children aged between 3 and 8 years of age diagnosed with amblyopia in association with either anisometropia or strabismus, or both, will be randomized to receive either a standardized or a personalized occlusion dose regimen. To avoid confounding by the known benefits of refractive correction, participants will not be randomized until they have completed an optical treatment phase. The primary study objective is to determine whether, at trial endpoint, participants receiving a personalized dosing strategy require fewer hours of occlusion than those in receipt of a standardized dosing strategy. Secondary objectives are to quantify the relationship between observed changes in visual acuity (logMAR, logarithm of the Minimum Angle of Resolution) with age, amblyopia type, and severity of amblyopic visual acuity deficit. DISCUSSION: This is the first randomized controlled trial of occlusion therapy for amblyopia to compare a treatment arm representative of current best practice with an arm representative of an entirely novel treatment regimen based on statistical modelling of previous trial outcome data. Should the personalized dosing strategy demonstrate superiority over the standardized dosing strategy, then its adoption into routine practice could bring practical benefits in reducing the duration of treatment needed to achieve an optimal outcome. TRIAL REGISTRATION: ISRCTN ISRCTN12292232.


Asunto(s)
Ambliopía/terapia , Vendajes , Privación Sensorial , Visión Ocular , Agudeza Visual , Factores de Edad , Ambliopía/diagnóstico , Ambliopía/fisiopatología , Niño , Preescolar , Protocolos Clínicos , Femenino , Humanos , Londres , Masculino , Recuperación de la Función , Proyectos de Investigación , Factores de Tiempo , Resultado del Tratamiento
15.
J Vis Exp ; (83): e51117, 2014 Jan 16.
Artículo en Inglés | MEDLINE | ID: mdl-24457314

RESUMEN

Tracing rare stable isotopes from plant material through the ecosystem provides the most sensitive information about ecosystem processes; from CO2 fluxes and soil organic matter formation to small-scale stable-isotope biomarker probing. Coupling multiple stable isotopes such as (13)C with (15)N, (18)O or (2)H has the potential to reveal even more information about complex stoichiometric relationships during biogeochemical transformations. Isotope labeled plant material has been used in various studies of litter decomposition and soil organic matter formation(1-4). From these and other studies, however, it has become apparent that structural components of plant material behave differently than metabolic components (i.e. leachable low molecular weight compounds) in terms of microbial utilization and long-term carbon storage(5-7). The ability to study structural and metabolic components separately provides a powerful new tool for advancing the forefront of ecosystem biogeochemical studies. Here we describe a method for producing (13)C and (15)N labeled plant material that is either uniformly labeled throughout the plant or differentially labeled in structural and metabolic plant components. Here, we present the construction and operation of a continuous (13)C and (15)N labeling chamber that can be modified to meet various research needs. Uniformly labeled plant material is produced by continuous labeling from seedling to harvest, while differential labeling is achieved by removing the growing plants from the chamber weeks prior to harvest. Representative results from growing Andropogon gerardii Kaw demonstrate the system's ability to efficiently label plant material at the targeted levels. Through this method we have produced plant material with a 4.4 atom%(13)C and 6.7 atom%(15)N uniform plant label, or material that is differentially labeled by up to 1.29 atom%(13)C and 0.56 atom%(15)N in its metabolic and structural components (hot water extractable and hot water residual components, respectively). Challenges lie in maintaining proper temperature, humidity, CO2 concentration, and light levels in an airtight (13)C-CO2 atmosphere for successful plant production. This chamber description represents a useful research tool to effectively produce uniformly or differentially multi-isotope labeled plant material for use in experiments on ecosystem biogeochemical cycling.


Asunto(s)
Andropogon/química , Andropogon/metabolismo , Isótopos de Carbono/química , Marcaje Isotópico/instrumentación , Isótopos de Nitrógeno/química , Isótopos de Carbono/metabolismo , Marcaje Isotópico/métodos , Isótopos de Nitrógeno/metabolismo
16.
Invest Ophthalmol Vis Sci ; 54(9): 6158-66, 2013 Sep 17.
Artículo en Inglés | MEDLINE | ID: mdl-23882695

RESUMEN

PURPOSE: Explore compliance with occlusion treatment of amblyopia in the Monitored and Randomized Occlusion Treatment of Amblyopia Studies (MOTAS and ROTAS), using objective monitoring. METHODS: Both studies had a three-phase protocol: initial assessment, refractive adaptation, and occlusion. In the occlusion phase, participants were instructed to dose for 6 hours/day (MOTAS) or randomized to 6 or 12 hour/day (ROTAS). Dose was monitored continuously using an occlusion dose monitor (ODM). RESULTS: One hundred and fifty-two patients (71 male, 81 female; 122 Caucasian, 30 non-Caucasian) of mean ± SD age 68 ± 18 months participated. Amblyopia was defined as an interocular acuity difference of at least 0.1 logMAR and was associated with anisometropia in 50, strabismus in 44, and both (mixed) in 58. Median duration of occlusion was 99 days (interquartile range 72 days). Mean compliance was 44%, mean proportion of days with no patch worn was 42%. Compliance was lower (39%) on weekends compared with weekdays (46%, P = 0.04), as was the likelihood of dosing at all (52% vs. 60%, P = 0.028). Compliance was lower when attendance was less frequent (P < 0.001) and with prolonged treatment duration (P < 0.001). Age, sex, amblyopia type, and severity were not associated with compliance. Mixture modeling suggested three subpopulations of patch day doses: less than 30 minutes; doses that achieve 30% to 80% compliance; and doses that achieve around 100% compliance. CONCLUSIONS: This study shows that compliance with patching treatment averages less than 50% and is influenced by several factors. A greater understanding of these influences should improve treatment outcome. (ClinicalTrials.gov number, NCT00274664).


Asunto(s)
Ambliopía/terapia , Cooperación del Paciente/estadística & datos numéricos , Privación Sensorial , Adaptación Ocular/fisiología , Ambliopía/fisiopatología , Vendajes , Niño , Preescolar , Femenino , Humanos , Masculino , Refracción Ocular/fisiología
17.
Adm Policy Ment Health ; 40(6): 541-54, 2013 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-23716144

RESUMEN

Social anxiety is highly prevalent but goes untreated. Although school-based CBT programs are efficacious when delivered by specialized psychologists, it is unclear whether school counselors can implement these interventions effectively, which is essential to promote sustainable school programs. We present an initial consultation strategy to support school counselor implementation of group CBT for social anxiety and an evaluation of counselors' treatment fidelity. Counselors were highly adherent to the treatment, but competence varied based on measurement. Counselors and consultants demonstrated good agreement for adherence, but relatively modest correspondence in competence ratings. We discuss future directions for school-based implementation efforts informed by these initial findings.


Asunto(s)
Actitud del Personal de Salud , Competencia Clínica , Terapia Cognitivo-Conductual/educación , Consultores/psicología , Consejo/normas , Servicios de Salud Escolar/normas , Adolescente , Terapia Cognitivo-Conductual/normas , Femenino , Humanos , Masculino , Trastornos Fóbicos/terapia , Psicoterapia de Grupo/educación , Psicoterapia de Grupo/normas
18.
J AAPOS ; 17(2): 166-73, 2013 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-23622448

RESUMEN

PURPOSE: To explore how stereoacuity changes in patients while they are being treated for amblyopia. METHODS: The Monitored Occlusion Treatment for Amblyopia Study (MOTAS) comprised 3 distinct phases. In the first phase, baseline, assessments of visual function were made to confirm the initial visual and binocular visual deficit. The second phase, refractive adaptation, now commonly termed "optical treatment," was an 18-week period of spectacle wear with measurements of logMAR visual acuity and stereoacuity with the Frisby test at weeks 0, 6, 12, and 18. In the third phase, occlusion, participants were prescribed 6 hours of patching per day. RESULTS: A total of 85 children were enrolled (mean age, 5.1 ± 1.5 years). In 21 children amblyopia was associated with anisometropia; in 29, with strabismus; and in 35, with both. At study entry, poor stereoacuity was associated with poor visual acuity (P < 0.001) in the amblyopic eye and greater angle of strabismus (P < 0.001). Of 66 participants, 25 (38%) who received refractive adaptation and 19 (29%) who received occlusion improved by at least one octave in stereoacuity, exceeding test-retest variability. Overall, 38 (45%) improved one or more octaves across both treatment phases. Unmeasureable stereoacuity was observed in 56 participants (66%) at study entry and in 37 (43%) at study exit. CONCLUSIONS: Stereoacuity improved for almost one half of the study participants. Improvement was observed in both treatment phases. Factors associated with poor or nil stereoacuity at study entry and exit were poor visual acuity of the amblyopic eye and large-angle strabismus.


Asunto(s)
Ambliopía/terapia , Anteojos , Privación Sensorial , Visión Binocular/fisiología , Agudeza Visual/fisiología , Ambliopía/fisiopatología , Niño , Preescolar , Estudios de Cohortes , Femenino , Humanos , Masculino , Errores de Refracción/fisiopatología , Errores de Refracción/terapia
19.
J Environ Qual ; 41(5): 1361-70, 2012.
Artículo en Inglés | MEDLINE | ID: mdl-23099927

RESUMEN

Biochar (BC) application to agricultural soils could potentially sequester recalcitrant C, increase N retention, increase water holding capacity, and decrease greenhouse gas (GHG) emissions. Biochar addition to soils can alter soil N cycling and in some cases decrease extractable mineral N (NO and NH) and NO emissions. These benefits are not uniformly observed across varying soil types, N fertilization, and BC properties. To determine the effects of BC addition on N retention and GHG flux, we added two sizes (>250 and <250 µm) of oak-derived BC (10% w/w) to two soils (aridic Argiustoll and aquic Haplustoll) with and without N fertilizer and measured extractable NO and NH and GHG efflux (NO, CO, and CH) in a 123-d laboratory incubation. Biochar had no effect on NO, NH, or NO in the unfertilized treatments of either soil. Biochar decreased cumulative extractable NO in N fertilized treatments by 8% but had mixed effects on NH. Greenhouse gas efflux differed substantially between the two soils, but generally with N fertilizer BC addition decreased NO 3 to 60%, increased CO 10 to 21%, and increased CH emissions 5 to 72%. Soil pH and total treatment N (soil + fertilizer + BC) predicted soil NO flux well across these two different soils. Expressed as CO equivalents, BC significantly reduced GHG emissions only in the N-fertilized silt loam by decreasing NO flux. In unfertilized soils, CO was the dominant GHG component, and the direction of the flux was mediated by positive or negative BC effects on soil CO flux. On the basis of our data, the use of BC appears to be an effective management strategy to reduce N leaching and GHG emissions, particularly in neutral to acidic soils with high N content.


Asunto(s)
Carbón Orgánico , Fertilizantes , Gases/análisis , Nitrógeno/análisis , Suelo/análisis , Metano/análisis , Nitratos/análisis , Compuestos de Amonio Cuaternario/análisis
20.
Child Adolesc Psychiatr Clin N Am ; 21(3): 655-68, 2012 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-22801000

RESUMEN

Anxiety disorders are the most common class of psychopathology among youth, yet many of these youngsters do not receive treatment. This is particularly concerning given the chronic course of anxiety disorders, which often lead to mood disorders, substance abuse, and serious impairment. Schools are an optimal venue for identifying anxious students and delivering mental health treatment given access to youth and ability to overcome various barriers to treatment. This article reviews four school-based treatments for anxiety disorders that have been evaluated in controlled trials. Discussion centers on feasibility, challenges to school-based implementation, and future research directions for this critical area.


Asunto(s)
Trastornos de Ansiedad/terapia , Terapia Cognitivo-Conductual/métodos , Servicios de Salud Escolar , Adolescente , Trastornos de Ansiedad/diagnóstico , Niño , Cultura , Humanos , Apoyo Social , Resultado del Tratamiento
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