Correction of Abnormalities Provoked by Long-Term Alcoholization with Hypoxic-Hyperoxic Training.

Free radical oxidation in the liver and skeletal muscles as well as stress behavior were examined in rats subjected to a gradual long-term alcoholization with elevated ethanol content from 10 to 40% followed by correction of alcohol-induced disturbances with hypoxic-hyperoxic training. The elevated plus-maze test revealed increased anxiety and appearance of risky behavior in alcoholized rats in the absence of changes in motor and orientation activity. In the liver and skeletal muscles of alcoholized rats, free radical oxidation processes were decompensated despite activation of antioxidant enzymes. Adaptation to intermittent hypoxia-hyperoxia during last two weeks of alcoholization exerted a protective effect against ethanol-induced oxidative stress: reduced anxious and risk behavior, normalized tissue tolerance of free radical oxidation processes, and restored the level of protective proteins.

Addition of Hyperoxic Component to Adaptation to Hypoxia Prevents Impairments Induced by Low Doses of Toxicants (Free Radical Oxidation and Proteins of HSP Family).

We studied the possibility of preventing disturbances caused by administration of low doses of toxicants by adaptation to interval hypoxia and hyperoxia. The preventive protective effect of adaptation to hypoxia-hyperoxia manifested in suppression of free radical oxidation, decrease in the levels of HIF-1α and inducible HOx-1, and improvement of tolerance to physical exercises.

Mechanisms of intracellular defense and activity of free radical oxidation in rat myocardium in the dynamics of chronic fluorine intoxication.

The mechanisms of intracellular defense and activity of free radical oxidation in the myocardium were studied in the dynamics of chronic fluorine intoxication. At the early stages of fluorine intoxication (day 3-week 3), the concentrations of defense proteins HIF-1α, HSC73, and HOx-2 and activity of the main metabolic enzymes increased, which promoted maintenance of cardiomyocyte structure and function at the normal physiological level. At late stages of fluorine intoxication (weeks 6 and 9), metabolic changes in the myocardium attest to high strain of the adaptive mechanisms.