Temporal distribution of intertidal macrozoobenthic assemblages in a Nanozostera noltii-dominated area (Lagoon of Venice).

We describe the temporal distribution of intertidal macrozoobenthic assemblages in a small marsh pond of the Lagoon of Venice colonized by the seagrass Nanozostera noltii (Hornemman) Tomlinson et Posluzny. Three stations ranging in the degree of N. noltii cover were selected about 100 m apart and sampled 9 times at regular intervals from March 1996 to March 1997. We applied the concepts of resistance and resilience to "natural stress" (e.g. extent of protection from seagrass meadows, exposure of macrozoobenthic assemblages to high temperatures in summer) with the aim to assess the stability of a community along a gradient of seagrass coverage. Results showed that the most structured and taxa-rich macrozoobenthic assemblage occurred at the station covered by a continuous stand of N. noltii, where permanent taxa (i.e. found in 100% of samples) were almost double than those found at the other stations. During the annual cycle, the macrozoobenthic assemblages showed a cyclical pattern, with temporal fluctuations increasing as they moved further away from the seagrass beds. We propose the role of N. noltii offering structural complexity and stability as the more probable explanation to the observed differences between stations in the intertidal assemblages.

Ongoing expansion of the worldwide invader Didemnum vexillum (Ascidiacea) in the Mediterranean Sea: high plasticity of its biological cycle promotes establishment in warm waters.

Non-indigenous ascidians are of particular concern to aquaculture industry and, paradoxically, the activities associated with it represent an important way to translocate these species worldwide. In 2012 a non-indigenous ascidian was found covering the oyster crops in the Ebro Delta (Western Mediterranean). We have identified the ascidian genetically and morphologically as Didemnum vexillum Kott, 2002. This finding indicates that the species is currently expanding its distribution in the Mediterranean Sea, as it has recently been found in the eastern basin (Venice, Adriatic Sea). Introduced populations of D. vexillum are found in temperate and cold waters worldwide, and a successful establishment in the Mediterranean implies a remarkable capacity of adaptability to warm, subtropical conditions. We assessed the life cycle (growth and reproduction) of the ascidian at the studied site. The species has a marked seasonal cycle, with regression in the warmest months and reappearance during winter. In spring D. vexillum reaches its maximum abundance, followed by a peak in reproduction just before regression. This cycle is reversed with respect to the one observed in colder waters, highlighting a plastic biological cycle of this invader and an hitherto unknown ability to establish itself in warm waters. We also analysed the genetic structure of the population of the Ebro Delta and the one established in the Lagoon of Venice using COI sequence data. The low genetic diversity in our samples (three haplotypes) was consistent with what is observed in the introduced populations worldwide. It is likely that the ascidian was introduced with oyster stock from bivalve cultures in the Atlantic French coasts, where the same three haplotypes have been reported. The high boating activity in the Ebro Delta makes further human-mediated transport of the species highly likely, and nearby fishing grounds can be severely affected if invaded. It is urgent to implement measures to prevent the continuous expansion of this ascidian pest in the Mediterranean.

Linking food web functioning and habitat diversity for an ecosystem based management: a Mediterranean lagoon case-study.

We propose a modelling approach relating the functioning of a transitional ecosystem with the spatial extension of its habitats. A test case is presented for the lagoon of Venice, discussing the results in the context of the application of current EU directives. The effects on food web functioning due to changes related to manageable and unmanageable drivers were investigated. The modelling procedure involved the use of steady-state food web models and network analysis, respectively applied to estimate the fluxes of energy associated with trophic interactions, and to compute indices of food web functioning. On the long term (hundred years) temporal scale, the model indicated that the expected loss of salt marshes will produce further changes at the system level, with a lagoon showing a decrease in the energy processing efficiency. On the short term scale, simulation results indicated that fishery management accompanied by seagrass restoration measures would produce a slight transition towards a more healthy system, with higher energy cycling, and maintaining a good balance between processing efficiency and resilience. Scenarios presented suggest that the effectiveness of short term management strategies can be better evaluated when contextualized in the long term trends of evolution of a system. We also remark the need for further studying the relationship between habitat diversity and indicators of food web functioning.

Animal-sediment relationships: evaluating the 'Pearson-Rosenberg paradigm' in Mediterranean coastal lagoons.

We investigated the applicability of the Pearson-Rosenberg (P-R) conceptual model describing a generalized pattern of response of benthic communities in relation to organic enrichment to Mediterranean Sea coastal lagoons. Consistent with P-R model predictions, benthic diversity and abundance showed two different peaks at low (>2.5-5 mg g(-1)) and high (>25-30 mg g(-1)) total organic carbon (TOC) ranges, respectively. We identified TOC thresholds indicating that risks of reduced benthic diversity should be relatively low at TOC valuesabout 28 mg g(-1), and intermediate at values in-between. Predictive ability within these ranges was high based on results of re-sampling simulation. While not a direct measure of causality, it is anticipated that these TOC thresholds should serve as a general screening-level indicator for evaluating the likelihood of reduced sediment quality and associated bioeffects in such eutrophic systems of the Mediterranean Sea.

Is the 1:4 elutriation ratio reliable? Ecotoxicological comparison of four different sediment: water proportions.

Methodological research was carried out to evaluate the discriminatory capability of three toxicity bioassays toward different elutriation ratios (1:4, 1:20, 1:50, and 1:200 sediment:water ratios). Samples from six sampling stations of the Lagoon of Venice have been investigated. The toxicity bioassay results (sea urchin Paracentrotus lividus Lmk sperm cell and embryo toxicity bioassays and bivalve mollusk Crassostrea gigas Thunberg embryo toxicity bioassays) have shown that elutriates generated from the widely used 1:4 ratio were less toxic than those from intermediate ratios (1:20 and 1:50).

Sediment toxicity assessment in the Lagoon of Venice (Italy) using Paracentrotus lividus (Echinodermata: Echinoidea) fertilization and embryo bioassays.

The capacity of two toxicity bioassays (fertilization and embryo toxicity tests) to discriminate sediment toxicity using the sea urchin Paracentrotus lividus was tested in five stations with different levels of pollution in the Lagoon of Venice. Two stations were located in estuarine sites, two in the industrial zone, and one in a site at the top of our quality gradient (reference). Elutriate was chosen as sediment matrix to assess the potential effects of bioavailable pollutants in the water column as a consequence of sediment resuspension (dredging and dumping, fishing gear, etc.). An experimental design based on Quality Assurance/Quality Control procedures (QA/QC) was adopted in order to set the methodological basis for an effective use of these bioassays in monitoring programs. Results revealed both higher embriotoxicity than spermiotoxicity in all stations and the efficacy of combined use of both toxicity bioassays in discriminating differing pollution/bioavailability between stations and periods. The good representativeness of the integrated sampling scheme and the standardization of all experimental phases yielded high precision of results. Clear Toxicity Fingerprints were evidenced for the investigated sites through the combined use of both bioassays. A good fit between ecotoxicological data and chemical contamination levels was found, except for unnatural sediment texture.

Insoluble biodegradative potential of the venice lagoon.

The sediment phase of the Venice lagoon within an area of shallow water, the Palude della Rosa, was studied and three insoluble enzymatic activities (cellulase, phosphatase and urease) were found to be linked to the inorganic phase. These immobilized enzymes were more resistant to environmental changes, even extreme ones, compared to their soluble counterparts. The evolution of their activity with pH, temperature and seasonal variation was investigated. While pH related activities showed the usual behaviour, the resistance to temperature was extremely high and seasonal variation was dependent on immobilization. These enzymatic activities could be used as a diagnostic factor for the ecosystem, since their presence is related to the nature of the waste products.