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I propose methods for reducing the number of exposures in incoherent digital holography with two polarization-sensitive phase-only spatial light modulators (IDH with TPP-SLMs). In IDH with TPP-SLMs, no polarization filters are required, and not only three-dimensional (3D), but polarization information is also obtained. However, seven exposures are required to conduct filter-free polarimetric incoherent holography. In this article, the optical designs and modified phase-shifting interferometry to reduce the number of recordings are described. IDH with TPP-SLMs has the potential for filter-free single-shot multidimensional incoherent holographic imaging.
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I propose a holography technique that detects both three-dimensional (3D) and polarization information on incoherent light without any polarization filters. Two polarization-sensitive phase-only spatial light modulators are used to generate self-interference holograms with different polarization directions. 3D images for different polarization directions are retrieved from the recorded polarization-multiplexed holograms, exploiting the developed phase-shifting interferometry and numerical refocusing. Moreover, light-use efficiency is improved in general incoherent holography because there is no polarization filter. Its validity is experimentally demonstrated.
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I propose a multidimension-multiplexed imaging method with which multiple physical quantities of light are simultaneously obtained as interference fringe images. The varieties of light are distinguished by exploiting the proposed phase-encoding technique. Neither measurements of point spread functions in advance, nor iterative calculations to derive multidimensional information, nor a laser light source is required. Multidimensional imaging of an object and simultaneous three-dimensional image recording of self-luminous light and light transmitted from an object are experimentally demonstrated. A palm-sized interferometer based on the proposed holography is developed for the experiments to show its portability and physical-filter-free multidimensional imaging ability without an antivibration structure.
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We propose single-path single-shot phase-shifting digital holographic microscopy (SSP-DHM) in which the quantitative phase information of an object wave is acquired without a laser light source. Multiple phase-shifted holograms are simultaneously obtained using a linear polarizer, a liquid crystal on a silicon spatial light modulator (LCoS-SLM), and a polarization-imaging camera. Complex amplitude imaging of a USAF1951 test target and phase imaging of transparent HeLa cells are performed to show its quantitative phase-imaging ability. We also conduct an experiment for the motion-picture imaging of transparent particles to highlight the single-shot imaging ability of SSP-DHM.
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Computational holography, encompassing computer-generated holograms and digital holography, utilizes diffraction calculations based on complex-valued operations and complex Fourier transforms. However, for some holographic applications, only real-valued holograms or real-valued diffracted results are required. This study proposes a real-valued diffraction calculation that does not require any complex-valued operation. Instead of complex-valued Fourier transforms, we employ a pure real-valued transform. Among the several real-valued transformations that have been proposed, we employ the Hartley transformation. However, our proposed method is not limited to this transformation, as other real-valued transformations can be utilized.
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Fresnel incoherent correlation holography (FINCH) was a milestone in incoherent holography. In this roadmap, two pathways, namely the development of FINCH and applications of FINCH explored by many prominent research groups, are discussed. The current state-of-the-art FINCH technology, challenges, and future perspectives of FINCH technology as recognized by a diverse group of researchers contributing to different facets of research in FINCH have been presented.
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We present color fluorescence imaging using an incoherent digital holographic technique in which holographic multiplexing of multiple wavelengths is exploited. Self-interference incoherent digital holography with a single-path in-line configuration and the computational coherent superposition scheme are adopted to obtain color holographic three-dimensional information of self-luminous objects with a monochrome image sensor and no mechanical scanning. We perform not only simultaneous color three-dimensional sensing of multiple self-luminous objects but also color fluorescence imaging of stained biological samples. Color fluorescence imaging with an improved point spread function is also demonstrated experimentally by adopting a Fresnel incoherent correlation holography system.
Asunto(s)
Holografía/instrumentación , Imagen Óptica/instrumentación , Complejos de Coordinación/química , Diseño de Equipo , Europio/química , Colorantes Fluorescentes/química , Células HeLa , Holografía/métodos , Humanos , Procesamiento de Imagen Asistido por Computador , Imagen Óptica/métodos , Terbio/químicaRESUMEN
We propose a phase-shifting interferometry technique using only two in-line phase-shifted self-interference holograms. There is no requirement for additional recording or estimation in the measurement. The proposed technique adopts a mathematical model for self-interference digital holography. The effectiveness of the proposed technique is demonstrated by experiments on incoherent digital holographic microscopy and color-multiplexed fluorescence digital holography with computational coherent superposition. Two-color-multiplexed four-step phase-shifting incoherent digital holography is realized for the first time, to the best of our knowledge, using the proposed technique.
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In this Letter, we propose spatially incoherent multiwavelength three-dimensional (3D) microscopy that exploits holographic multiplexing and is based on computational coherent superposition (CCS). The proposed microscopy generates spatially incoherent wavelength-multiplexed self-interference holograms with a multiband-pass filter and spatially and temporally incoherent light diffracted from specimens. Selective extractions of 3D spatial information at multiple wavelengths from the holograms are realized using the CCS scheme. We constructed fully mechanical-motion-free holographic multiwavelength 3D microscopy systems and conducted experiments to demonstrate the microscopy.
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We propose multiwavelength-multiplexed phase-shifting incoherent color digital holography. In this technique, a monochrome image sensor records wavelength-multiplexed, phase-shifted, and incoherent holograms, and a phase-shifting interferometry technique selectively extracts object waves at multiple wavelengths from the several recorded holograms. Spatially incoherent light that contains multiple wavelengths illuminates objects, and multiwavelength-incoherent object waves are simultaneously obtained without using any wavelength filters. Its effectiveness is experimentally demonstrated for transparent and reflective objects.
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We propose and demonstrate a multicolor single-pixel digital holography technique. The intensity and phase images of an object are simultaneously obtained from the time-sequence intensity data captured using a single-pixel photodetector. Moreover, phase-structured light and phase-shifting interferometry are implemented using only a spatial light modulator without any mechanical movements. The adopted configuration dramatically simplifies the optical setup by removing both imaging optics and the resultant chromatic aberration. The effectiveness of the proposed technique is demonstrated numerically and experimentally.
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We propose multiwavelength in-line phase-shifting digital holography with a monochrome image sensor, single reference arm, and no mechanical scanning. We use phase-shifting interferometry selectively extracting wavelength information as a method to obtain multiwavelength object waves separately from wavelength-multiplexed phase-shifted holograms. By exploiting a liquid crystal on silicon spatial light modulator with a wide phase-modulation range as an electrically driven phase shifter in this interferometry, we remove mechanically moving parts during phase shifting. Its effectiveness is experimentally demonstrated.
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We propose a high-speed image-reconstruction algorithm for a spatially multiplexed image that is obtained by spatial frequency-division multiplexing. The algorithm utilizes smoothing and does not require any Fourier transform (FT) or iterative procedure to extract the desired information selectively from a single image. Numerical and experimental results show its validity and color holographic imaging ability. Calculation time of the proposed is less than a tenth of that of the FT method when using a central processing unit and an image sensor with four megapixels. Furthermore, throughput that is three times that of the FT method can be achieved in the case of using a graphics processing unit.
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In this review, we introduce digital holographic techniques and recent progress in multidimensional sensing by using digital holography. Digital holography is an interferometric imaging technique that does not require an imaging lens and can be used to perform simultaneous imaging of multidimensional information, such as three-dimensional structure, dynamics, quantitative phase, multiple wavelengths and polarization state of light. The technique can also obtain a holographic image of nonlinear light and a three-dimensional image of incoherent light with a single-shot exposure. The holographic recording ability of this technique has enabled a variety of applications.
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We propose multiwavelength in-line digital holography with wavelength-multiplexed phase-shifted holograms and arbitrary symmetric phase shifts. We use phase-shifting interferometry selectively extracting wavelength information to reconstruct multiwavelength object waves separately from wavelength-multiplexed monochromatic images. The proposed technique obtains systems of equations for real and imaginary parts of multiwavelength object waves from the holograms by introducing arbitrary symmetric phase shifts. Then, the technique derives each complex amplitude distribution of each object wave selectively and analytically by solving the two systems of equations. We formulate the algorithm in the case of an arbitrary number of wavelengths and confirm its validity numerically and experimentally in the cases where the number of wavelengths is two and three.
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We propose single-shot multiwavelength digital holography with an extremely large incident angle and show the digital recording of multiple object waves at multiple wavelengths with an angle of more than 40 degrees and no beam combiner to generate interference light. Both the avoidance of the crosstalk between the object waves at different wavelengths and the space-bandwidth extension are simultaneously achieved with a single-shot exposure of a monochromatic image sensor and a reference beam even when the wavelength difference between the object waves is small. An extremely large angle can be set by utilizing the signal theory. An angle of up to 40.6 degrees was introduced, interference fringes with an 818 nm period at the wavelength of 532 nm were generated, and an image sensor recorded a two-wavelength-multiplexed hologram. Resolution improvement was experimentally demonstrated using two-wavelength digital holography with the wavelengths of 640 and 532 nm.
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Dual-wavelength phase-shifting digital holography that selectively extracts wavelength information from five wavelength-multiplexed holograms is presented. Specific phase shifts for respective wavelengths are introduced to remove the crosstalk components and extract only the object wave at the desired wavelength from the holograms. Object waves in multiple wavelengths are selectively extracted by utilizing 2π ambiguity and the subtraction procedures based on phase-shifting interferometry. Numerical results show the validity of the proposed technique. The proposed technique is also experimentally demonstrated.
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Holografía/métodos , Holografía/instrumentación , Interferometría , Rayos Láser , Modelos TeóricosRESUMEN
We propose phase-shift digital holography (DH) with a one-bit image sensor. In this method, the propagating complex field from an object is binarized by a one-bit sensor using a phase-shifter. The complex field on the hologram plane is then calculated with the one-bit image data. The object field is recovered via Fresnel back-propagation of the calculated hologram and filtering to suppress some artifacts caused by the binarization. The concept was demonstrated in preliminary experiments by using a synthetically binarized hologram with single-shot and multi-shot phase-shift DH.
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We propose a single-shot phase-unwrapping method using two wavelengths in parallel phase-shifting digital holography (PPSDH). The proposed method enables one to solve the phase ambiguity problem in PPSDH. We conducted an experiment of the proposed method using two lasers whose wavelengths are 473 and 532 nm. An object having about 1.9 µm step, which is 7.1 times larger than the half wavelength of one of the lasers (266 nm), was fabricated by using vapor deposition of aluminum. Single-shot measurement of the height of the object was successfully demonstrated, and the validity of the proposed method was verified.
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A superresolution method for interference fringes obtained by parallel four-step phase-shifting digital holography is proposed. A complex amplitude distribution of an object wave is derived from a recorded hologram by parallel phase-shifting interferometry using two pixels without any interpolation procedures. Multiple distributions are derived by changing one of the two pixels when conducting phase-shifting interferometry. The angular spectrum distribution of the object wave is obtained by both the Fourier transforms and synthesis of the spectrum distribution from the Fourier-transformed images in the spatial frequency domain. Available space bandwidth is extended to half of that of an image sensor.