RESUMEN
In parkinsonism, subthalamic nucleus (STN) electrical deep brain stimulation (DBS) improves symptoms, but may be associated with side effects. Adaptive DBS (aDBS), which enables modulation of stimulation, may limit side effects, but limited information is available about clinical effectiveness and efficaciousness. We developed a brain-machine interface for aDBS, which enables modulation of stimulation parameters of STN-DBS in response to γ2 band activity (80-200 Hz) of local field potentials (LFPs) recorded from the primary motor cortex (M1), and tested its effectiveness in parkinsonian monkeys. We trained two monkeys to perform an upper limb reaching task and rendered them parkinsonian with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine. Bipolar intracortical recording electrodes were implanted in the M1, and a recording chamber was attached to access the STN. In aDBS, the M1 LFPs were recorded, filtered into the γ2 band, and discretized into logic pulses by a window discriminator, and the pulses were used to modulate the interval and amplitude of DBS pulses. In constant DBS (cDBS), constant stimulus intervals and amplitudes were used. Reaction and movement times during the task were measured and compared between aDBS and cDBS. The M1-γ2 activities were increased before and during movements in parkinsonian monkeys and these activities modulated the aDBS pulse interval, amplitude, and dispersion. With aDBS and cDBS, reaction and movement times were significantly decreased in comparison to DBS-OFF. The electric charge delivered was lower with aDBS than cDBS. M1-γ2 aDBS in parkinsonian monkeys resulted in clinical benefits that did not exceed those from cDBS. However, M1-γ2 aDBS achieved this magnitude of benefit for only two thirds of the charge delivered by cDBS. In conclusion, M1-γ2 aDBS is an effective therapeutic approach which requires a lower electrical charge delivery than cDBS for comparable clinical benefits.
Asunto(s)
Estimulación Encefálica Profunda , Corteza Motora , Trastornos Parkinsonianos , Núcleo Subtalámico , Animales , Estimulación Encefálica Profunda/métodos , Haplorrinos , Corteza Motora/fisiología , Núcleo Subtalámico/fisiologíaRESUMEN
The changes in neuronal firing activity in the primary motor cortex (M1) and supplementary motor area (SMA) were compared in monkeys rendered parkinsonian by treatment with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine. The neuronal dynamic was characterized using mathematical tools defined in different frameworks (rate, oscillations or complex patterns). Then, and for each cortical area, multivariate and discriminate analyses were further performed on these features to identify those important to differentiate between the normal and the pathological neuronal activity. Our results show a different order in the importance of the features to discriminate the pathological state in each cortical area which suggests that the M1 and the SMA exhibit dissimilarities in their neuronal alterations induced by parkinsonism. Our findings highlight the need for multiple mathematical frameworks to best characterize the pathological neuronal activity related to parkinsonism. Future translational studies are warranted to investigate the causal relationships between cortical region-specificities, dominant pathological hallmarks and symptoms.
Asunto(s)
Potenciales de Acción , Corteza Motora/fisiopatología , Neuronas/fisiología , Trastornos Parkinsonianos/fisiopatología , 1-Metil-4-fenil-1,2,3,6-Tetrahidropiridina , Potenciales de Acción/fisiología , Animales , Ondas Encefálicas , Femenino , Modelos Lineales , Macaca fuscata , Masculino , Microelectrodos , Análisis Multivariante , Dinámicas no Lineales , Análisis de Componente Principal , Procesamiento de Señales Asistido por ComputadorRESUMEN
The present study compares the cortical local field potentials (LFPs) in the primary motor cortex (M1) and the supplementary motor area (SMA) of non-human primates rendered Parkinsonian with administration of dopaminergic neurotoxin, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine. The dynamic of the LFPs was investigated under several mathematical frameworks and machine learning was used to discriminate the recordings based on these features between healthy, parkinsonian with off-medication and parkinsonian with on-medication states. The importance of each feature in the discrimination process was further investigated. The dynamic of the LFPs in M1 and SMA was affected regarding its variability (time domain analysis), oscillatory activities (frequency domain analysis) and complex patterns (non-linear domain analysis). Machine learning algorithms achieved accuracy near 0.90 for comparisons between conditions. The TreeBagger algorithm provided best accuracy. The relative importance of these features differed with the cortical location, condition and treatment. Overall, the most important features included beta oscillation, fractal dimension, gamma oscillation, entropy and asymmetry of amplitude fluctuation. The importance of features in discriminating between normal and pathological states, and on- or off-medication states depends on the pair-comparison and it is region-specific. These findings are discussed regarding the refinement of current models for movement disorders and the development of on-demand therapies.
Asunto(s)
Corteza Motora , Trastornos Parkinsonianos , Animales , Macaca mulatta , Aprendizaje AutomáticoRESUMEN
The banking of human leukocyte antigen (HLA)-homozygous-induced pluripotent stem cells (iPSCs) is considered a future clinical strategy for HLA-matched cell transplantation to reduce immunological graft rejection. Here we show the efficacy of major histocompatibility complex (MHC)-matched allogeneic neural cell grafting in the brain, which is considered a less immune-responsive tissue, using iPSCs derived from an MHC homozygous cynomolgus macaque. Positron emission tomography imaging reveals neuroinflammation associated with an immune response against MHC-mismatched grafted cells. Immunohistological analyses reveal that MHC-matching reduces the immune response by suppressing the accumulation of microglia (Iba-1+) and lymphocytes (CD45+) into the grafts. Consequently, MHC-matching increases the survival of grafted dopamine neurons (tyrosine hydroxylase: TH+). The effect of an immunosuppressant, Tacrolimus, is also confirmed in the same experimental setting. Our results demonstrate the rationale for MHC-matching in neural cell grafting to the brain and its feasibility in a clinical setting.Major histocompatibility complex (MHC) matching improves graft survival rates after organ transplantation. Here the authors show that in macaques, MHC-matched iPSC-derived neurons provide better engraftment in the brain, with a lower immune response and higher survival of the transplanted neurons.
Asunto(s)
Neuronas Dopaminérgicas/trasplante , Rechazo de Injerto/inmunología , Antígenos HLA/genética , Células Madre Pluripotentes Inducidas/trasplante , Complejo Mayor de Histocompatibilidad/inmunología , Animales , Neuronas Dopaminérgicas/inmunología , Femenino , Haplotipos , Inmunohistoquímica , Linfocitos/inmunología , Macaca , Masculino , Microglía/inmunología , Tomografía de Emisión de PositronesRESUMEN
Induced pluripotent stem cells (iPS cells) are a promising source for a cell-based therapy to treat Parkinson's disease (PD), in which midbrain dopaminergic neurons progressively degenerate. However, long-term analysis of human iPS cell-derived dopaminergic neurons in primate PD models has never been performed to our knowledge. Here we show that human iPS cell-derived dopaminergic progenitor cells survived and functioned as midbrain dopaminergic neurons in a primate model of PD (Macaca fascicularis) treated with the neurotoxin MPTP. Score-based and video-recording analyses revealed an increase in spontaneous movement of the monkeys after transplantation. Histological studies showed that the mature dopaminergic neurons extended dense neurites into the host striatum; this effect was consistent regardless of whether the cells were derived from patients with PD or from healthy individuals. Cells sorted by the floor plate marker CORIN did not form any tumours in the brains for at least two years. Finally, magnetic resonance imaging and positron emission tomography were used to monitor the survival, expansion and function of the grafted cells as well as the immune response in the host brain. Thus, this preclinical study using a primate model indicates that human iPS cell-derived dopaminergic progenitors are clinically applicable for the treatment of patients with PD.
Asunto(s)
Modelos Animales de Enfermedad , Neuronas Dopaminérgicas/citología , Neuronas Dopaminérgicas/trasplante , Células Madre Pluripotentes Inducidas/citología , Enfermedad de Parkinson/patología , Enfermedad de Parkinson/terapia , Medicina Regenerativa/métodos , 1-Metil-4-fenil-1,2,3,6-Tetrahidropiridina , Adulto , Anciano , Anciano de 80 o más Años , Animales , Proliferación Celular , Supervivencia Celular , Neuronas Dopaminérgicas/inmunología , Humanos , Macaca fascicularis , Imagen por Resonancia Magnética , Masculino , Mesencéfalo/citología , Movimiento , Neostriado/citología , Neuritas , Enfermedad de Parkinson/etiología , Enfermedad de Parkinson/fisiopatología , Tomografía de Emisión de Positrones , Serina Endopeptidasas/análisis , Serina Endopeptidasas/metabolismoRESUMEN
Activity patterns of projection neurons in the putamen were investigated in behaving monkeys. Stimulating electrodes were implanted chronically into the proximal (MI(proximal)) and distal (MI(distal)) forelimb regions of the primary motor cortex (MI) and the forelimb region of the supplementary motor area (SMA). Cortical inputs to putaminal neurons were identified by excitatory orthodromic responses to stimulation of these motor cortices. Then, neuronal activity was recorded during the performance of a goal-directed reaching task with delay. Putaminal neurons with inputs from the MI and SMA showed different activity patterns, i.e., movement- and delay-related activity, during task performance. MI-recipient neurons increased activity in response to arm-reach movements, whereas SMA-recipient neurons increased activity during delay periods, as well as during movements. The activity pattern of MI + SMA-recipient neurons was of an intermediate type between those of MI- and SMA-recipient neurons. Approximately one-half of MI(proximal)-, SMA-, and MI + SMA-recipient neurons changed activities before the onset of movements, whereas a smaller number of MI(distal)- and MI(proximal + distal)-recipient neurons did. Movement-related activity of MI-recipient neurons was modulated by target directions, whereas SMA- and MI + SMA-recipient neurons had a lower directional selectivity. MI-recipient neurons were located mainly in the ventrolateral part of the caudal aspect of the putamen, whereas SMA-recipient neurons were located in the dorsomedial part. MI + SMA-recipient neurons were found in between. The present results suggest that a subpopulation of putaminal neurons displays specific activity patterns depending on motor cortical inputs. Each subpopulation receives convergent or nonconvergent inputs from the MI and SMA, retains specific motor information, and sends it to the globus pallidus and the substantia nigra through the direct and indirect pathways of the basal ganglia.
Asunto(s)
Corteza Motora/fisiología , Neuronas/fisiología , Estimulación Luminosa/métodos , Desempeño Psicomotor/fisiología , Putamen/fisiología , Tiempo de Reacción/fisiología , Animales , Femenino , Haplorrinos , Macaca , Masculino , Vías Nerviosas/fisiología , Distribución AleatoriaRESUMEN
Macaque monkeys have a highly evolved visual system comparable to that of humans. One of the important visual functions is performing discriminations among biologically significant objects such as food or heterosexual partners. In the present study, we examined whether rhesus monkeys could categorize two-dimensional images related to food or gender using a visual discrimination task. Three rhesus monkeys were trained to make distinctions of food from non-food items, and between male and female monkeys, using 60 or more different pictures of each category. After more than 9 months of training, the monkeys discriminated a variety of foods from non-food and different males from females with more than 80% accuracy, even when the stimuli were used for the first time or presented only once in a session. The proportion of correct responses and response latencies showed better performance in discrimination of food/non-food than that of gender. The results suggest that rhesus monkeys are able to perform visual discrimination of highly abstract biologically significant categories with better performance in a food-related category than a gender-related one, using two-dimensional visual information.