The development of alternative techniques to efficiently inactivate bacterial suspensions is crucial to prevent transmission of waterborne illness, particularly when commonly used techniques such as heating, filtration, chlorination, or ultraviolet treatment are not practical or feasible. We examine the effect of MHz-order acoustic wave irradiation in the form of surface acoustic waves (SAWs) on Gram-positive (Escherichia coli) and Gram-negative (Brevibacillus borstelensis and Staphylococcus aureus) bacteria suspended in water droplets. A significant increase in the relative bacterial load reduction of colony-forming units (up to 74%) can be achieved by either increasing (1) the excitation power, or, (2) the acoustic treatment duration, which we attributed to the effect of the acoustic radiation force exerted on the bacteria. Consequently, by increasing the maximum pressure amplitude via a hybrid modulation scheme involving a combination of amplitude and pulse-width modulation, we observe that the bacterial inactivation efficiency can be further increased by approximately 14%. By combining this scalable acoustic-based bacterial inactivation platform with plasma-activated water, a 100% reduction in E. coli is observed in less than 10 mins, therefore demonstrating the potential of the synergistic effects of MHz-order acoustic irradiation and plasma-activated water as an efficient strategy for water decontamination.
Bacteria , Escherichia coli , Bacteria/radiation effects , Sound , Acoustics , Water
Plasma treatment constitutes an efficient method for chemical-free disinfection. A spray-based system for dispensing plasma-activated aerosols onto surfaces would facilitate disinfection of complex and/or hidden surfaces inaccessible to direct line-of-sight (for example, UV) methods. The complexity and size of current plasma generators (for example, plasma jet and cometary plasma systems)-which prohibit portable operation, together with the short plasma lifetimes, necessitate a miniaturized in situ technique in which a source can be simultaneously activated and administered on-demand onto surfaces. Here, we demonstrate this possibility by combining two nanoscale technologies for plasma and aerosol generation into an integrated device that is sufficiently small and lightweight. Plasma is generated on a carpet of zinc oxide nanorods comprising a nanoneedle ensemble, which when raised to a high electric potential, constitutes a massive point charge array with near-singular electric fields to effect atmospheric breakdown. The plasma is then used to activate water transported through an underlying capillary wick, that is subsequently aerosolized under MHz-order surface acoustic waves. We show that the system, besides being amenable to miniaturization and hence integration into a chipscale device, leads to a considerable improvement in plasma-activation over its macroscale cometary discharge predecessor, with up to 20% and 127% higher hydrogen peroxide and nitrite ion concentrations that are respectively generated in the plasma-activated aerosols. This, in turn, leads to a 67% reduction in the disinfection time to achieve 95% bacterial load reduction, therefore demonstrating the potential of the technology as an efficient portable platform for on-demand field-use surface disinfection.
We investigate a hybrid treatment-consisting of an atmospheric pressure plasma pretreatment, followed by an MHz surface acoustic waves (SAWs) treatment with either de-ionized (DI) water or plasma activated water (PAW)-on mung beans to accelerate the germination process, as mung bean sprout is one of the important food staples. For the early growth rate (after 320 min), we observe that the hybrid treatment with PAW can lead to approximately 217% higher moisture content for the treated beans when compared with that without hybrid treatment. Additionally, the hybrid-treated beans germinate in around 120 min, while the untreated beans germinate only in around 420 min, that is, 3.5-fold faster for treated beans. This can be attributed to the dominant effect of SAW that accelerates stage 1 water absorption process and the effect of direct plasma and PAW that promote stage 2 metabolism process, leading to the enhancement in stage 3 germination process in early growth rate. For the post growth rate (after 24 h), we observe that the hybrid treatment with DI water can lead to an approximately 44.20% in higher moisture and 71.17% in radicle length when compared with untreated beans. Interestingly, the hybrid treatment with PAW, on the other hand, is observed to have an adverse effect on germination after 24 h, that is, approximately 14.51% lower in moisture content and 43.49% lower in radicle length for the hybrid-treated beans with PAW when compared with that with DI water.
Fabaceae , Vigna , Germination , Seeds , Sound
We reveal a unique mechanism by which pure water can be dissociated to form free radicals without requiring catalysts, electrolytes, or electrode contact by means of high-frequency nanometer-amplitude electromechanical surface vibrations in the form of surface acoustic waves (SAWs) generated on a piezoelectric substrate. The physical undulations associated with these mechanical waves, in concert with the evanescent electric field arising from the piezoelectric coupling, constitute half-wavelength "nanoelectrochemical cells" in which liquid is trapped within the SAW potential minima with vertical dimensions defined by the wave amplitude (â¼10 nm), thereby forming highly confined polarized regions with intense electric field strengths that enable the breakdown of water. The ions and free radicals that are generated rapidly electromigrate under the high field intensity in addition to being convectively transported away from the cells by the bulk liquid recirculation generated by the acoustic excitation, thereby overcoming mass transport limitations that lead to ion recombination.
The presence of reactive species in plasma-activated water is known to induce oxidative stresses in bacterial species, which can result in their inactivation. By integrating a microfludic chipscale nebulizer driven by surface acoustic waves (SAWs) with a low-temperature atmospheric plasma source, we demonstrate an efficient technique for in situ production and application of plasma-activated aerosols for surface disinfection. Unlike bulk conventional systems wherein the water is separately batch-treated within a container, we show in this work the first demonstration of continuous plasma-treatment of water as it is transported through a paper strip from a reservoir onto the chipscale SAW device. The significantly larger surface area to volume ratio of the water within the paper strip leads to a significant reduction in the duration of the plasma-treatment, while maintaining the concentration of the reactive species. The subsequent nebulization of the plasma-activated water by the SAW then allows the generation of plasma-activated aerosols, which can be directly sprayed onto the contaminated surface, therefore eliminating the storage of the plasma-activated water and hence circumventing the typical limitation in conventional systems wherein the concentration of the reactive species diminishes over time during storage, resulting in a reduction in the efficacy of bacterial inactivation. In particular, we show up to 96% reduction in Escherichia coli colonies through direct spraying with the plasma-activated aerosols. This novel, low-cost, portable and energy-efficient hybrid system necessitates only minimal maintenance as it only requires the supply of tap water and battery power for operation, and is thus suitable for decontamination in home environments.
Disinfection , Sound , Aerosols , Bacteria , Water
There is a pressing need for efficient biological treatment systems for the removal of organic compounds in greywater given the rapid increase in household wastewater produced as a consequence of rapid urbanisation. Moreover, proper treatment of greywater allows its reuse that can significantly reduce the demand for freshwater supplies. Herein, we demonstrate the possibility of enhancing the removal efficiency of solid contaminants from greywater using MHz-order surface acoustic waves (SAWs). A key distinction of the use of these high frequency surface acoustic waves, compared to previous work on its lower frequency (kHz order) bulk ultrasound counterpart for wastewater treatment, is the absence of cavitation, which can inflict considerable damage on bacteria, thus limiting the intensity and duration, and hence the efficiency enhancement, associated with the acoustic exposure. In particular, we show that up to fivefold improvement in the removal efficiency can be obtained, primarily due to the ability of the acoustic pressure field in homogenizing and reducing the size of bacterial clusters in the sample, therefore providing a larger surface area that promotes greater bacteria digestion. Alternatively, the SAW exposure allows the reduction in the treatment duration to achieve a given level of removal efficiency, thus facilitating higher treatment rates and hence processing throughput. Given the low-cost of the miniature chipscale platform, these promising results highlight its possibility for portable greywater treatment for domestic use or for large-scale industrial wastewater processing through massive parallelization.
Waste Disposal, Fluid , Wastewater , Bacteria , Sound
Rayleigh surface acoustic waves (SAWs) have been demonstrated as a powerful and effective means for driving a wide range of microfluidic actuation processes. Traditionally, SAWs have been generated on piezoelectric substrates, although the cost of the material and the electrode deposition process makes them less amenable as low-cost and disposable components. As such, a "razor-and-blades" model that couples the acoustic energy of the SAW on the piezoelectric substrate through a fluid coupling layer and into a low-cost and, hence, disposable silicon superstrate on which various microfluidic processes can be conducted has been proposed. Nevertheless, it was shown that only bulk vibration in the form of Lamb waves can be excited in the superstrate, which is considerably less efficient and flexible in terms of microfluidic functionality compared to its surface counterpart, that is, the SAW. Here, we reveal an extremely simple way that quite unexpectedly and rather nonintuitively allows SAWs to be generated on the superstrate-by coating the superstrate with a thin gold layer. In addition to verifying the existence of the SAW on the coated superstrate, we carry out finite-difference time domain numerical simulations that not only confirm the experimental observations but also facilitate an understanding of the surprising difference that the coating makes. Finally, we elucidate the various power-dependent particle concentration phenomena that can be carried out in a sessile droplet atop the superstrate and show the possibility for simply carrying out rapid and effective microcentrifugation-a process that is considerably more difficult with Lamb wave excitation on the superstrate.
The ability to drive efficient micromixing on a microfluidic platform is crucial for a wide range of lab-on-a-chip applications. Here, we investigate the ability of acoustic waves generated on different geometric surfaces (concave and convex) to enhance the micromixing efficiency in droplet acoustomicrofluidic systems, and, concomitantly, to reduce the power consumption in these devices for a given performance requirement. Quite counterintuitively, we observe that although the acoustic streaming velocity, which scaled inversely with the droplet size, tended to be generally lower (by approximately 45%) when the flow is generated by transducers with convex surfaces compared to those with concave surfaces, the mixing efficiency is disproportionately higher: compared to pure diffusional mixing in the absence of the acoustic forcing, the mixing efficiency due to the acoustically driven convection increased by up to 25% and 43% on these respective surfaces. As such, the mixing enhancement cannot simply be attributed to an increase in the convective flow arising from the acoustic forcing. Rather, we observe the mixing enhancement to be due to the stronger chaotic advection arising in the transducer with the convex surface due to its diverging acoustic field into the droplet.
Seeds, which are high in protein and essential nutrients, must go through a hydration process before consumption. The ability to rapidly increase water absorption can significantly reduce the soaking time as well as the amount of energy needed for cooking seeds. Many studies in the literature employ high-power (102 W) low-frequency (104 Hz) ultrasound; although their results are very promising where more than 100% increase in water content can be obtained between the treated and untreated seeds, the high-power and low-frequency ultrasound often causes acoustic cavitation under high intensity, which can severely disrupt the cell walls and damage the seeds. In our study, however, we demonstrate that treating the seeds via a miniature surface acoustic wave device, which operates at low-power (100 W) and high-frequency (107 Hz) range, gives rise to a higher water absorption rate without the acoustic cavitations. By comparing the water content between the treated and untreated seeds, an increase of up to 2600% (for chickpeas) and 6350% (for mung bean) can be obtained after 60 min. A significantly higher water absorption in mung beans can be attributed to the larger pore size when compared with the acoustic wavelength in water, enabling an efficient transmission of acoustic wave inside the pores. Our results also indicate that the germination time can be reduced by half for treated seeds as compared to the untreated seeds.
The Miniaturised Lab-on-a-Disc (miniLOAD) platform, which utilises surface acoustic waves (SAWs) to drive the rotation of thin millimeter-scale discs on which microchannels can be fabricated and hence microfluidic operations can be performed, offers the possibility of miniaturising its larger counterpart, the Lab-on-a-CD, for true portability in point-of-care applications. A significant limitation of the original miniLOAD concept, however, is that it does not allow for flexible control over the disc rotation direction and speed without manual adjustment of the disc's position, or the use of multiple devices to alter the SAW frequency. In this work, we demonstrate the possibility of achieving such control with the use of tapered interdigitated transducers to confine a SAW beam such that the localised acoustic streaming it generates imparts a force, through hydrodynamic shear, at a specific location on the disc. Varying the torque that arises as a consequence by altering the input frequency to the transducers then allows the rotational velocity and direction of the disc to be controlled with ease. We derive a simple predictive model to illustrate the principle by which this occurs, which we find agrees well with the experimental measurements.
We exploit the possibility of enhancing the molecular transport of liquids through graphene films using amplitude modulated surface acoustic waves (SAWs) to demonstrate effective and efficient nanoparticle filtration. The use of the SAW, which is an extremely efficient means for driving microfluidic transport, overcomes the need for the large mechanical pumps required to circumvent the large pressure drops encountered in conventional membranes for nanoparticle filtration. 100% filtration efficiency was obtained for micron-dimension particulates, decreasing to only 95% for the filtration of particles of tens of nanometers in dimension, which is comparable to that achieved with other methods. To circumvent clogging of the film, which is typical with all membrane filters, a backwash operation to flush the nanoparticles is incorporated simply by reversing the SAW-induced flow such that 98% recovery of the initial filtration rate is recovered. Given these efficiencies, together with the low cost and compact size of the chipscale SAW devices, we envisage the possibility of scaling out the process by operating a large number of devices in parallel to achieve typical industrial-scale throughputs with potential benefits in terms of substantially lower capital, operating and maintenance costs.
The ability to drive microcentrifugation for efficient micromixing and particle concentration and separation on a microfluidic platform is critical for a wide range of lab-on-a-chip applications. In this work, we investigate the use of amplitude modulation to enhance the efficiency of the microcentrifugal recirculation flows in surface acoustic wave microfluidic systems, thus concomitantly reducing the power consumption in these devices for a given performance requirement-a crucial step in the development of miniaturized, integrated circuits for true portable functionality. In particular, we show that it is possible to obtain an increase of up to 60% in the acoustic streaming velocity in a microdroplet with kHz order modulation frequencies due to the intensification in Eckart streaming; the streaming velocity is increasing as the modulation index is increased. Additionally, we show that it is possible to exploit this streaming enhancement to effect improvements in the speed of particle concentration by up to 70% and the efficiency of micromixing by 50%, together with a modest decrease in the droplet temperature.
The deposition of a thin graphene film atop a chip scale piezoelectric substrate on which surface acoustic waves are excited is observed to enhance its performance for fluid transport and manipulation considerably, which can be exploited to achieve further efficiency gains in these devices. Such gains can then enable complete integration and miniaturization for true portability for a variety of microfluidic applications across drug delivery, biosensing and point-of-care diagnostics, among others, where field-use, point-of-collection or point-of-care functionality is desired. In addition to a first demonstration of vibration-induced molecular transport in graphene films, we show that the coupling of the surface acoustic wave gives rise to antisymmetric Lamb waves in the film which enhance molecular diffusion and hence the flow through the interstitial layers that make up the film. Above a critical input power, the strong substrate vibration displacement can also force the molecules out of the graphene film to form a thin fluid layer, which subsequently destabilizes and breaks up to form a mist of micron dimension aerosol droplets. We provide physical insight into this coupling through a simple numerical model, verified through experiments, and show several-fold improvement in the rate of fluid transport through the film, and up to 55% enhancement in the rate of fluid atomization from the film using this simple method.
We investigate the enhancement of heat transfer in the nucleate boiling regime by inducing high frequency acoustic waves (f â¼ 10(6) Hz) on the heated surface. In the experiments, liquid droplets (deionized water) are dispensed directly onto a heated, vibrating substrate. At lower vibration amplitudes (ξs â¼ 10(-9) m), the improved heat transfer is mainly due to the detachment of vapor bubbles from the heated surface and the induced thermal mixing. Upon increasing the vibration amplitude (ξs â¼ 10(-8) m), the heat transfer becomes more substantial due to the rapid bursting of vapor bubbles happening at the liquid-air interface as a consequence of capillary waves travelling in the thin liquid film between the vapor bubble and the air. Further increases then lead to rapid atomization that continues to enhance the heat transfer. An acoustic wave displacement amplitude on the order of 10(-8) m with 10(6) Hz order frequencies is observed to produce an improvement of up to 50% reduction in the surface temperature over the case without acoustic excitation.
Suppressing the Leidenfrost effect can significantly improve heat transfer from a heated substrate to a droplet above it. In this work, we demonstrate that by generating high frequency acoustic wave in the droplet, at sufficient vibration displacement amplitudes, the Leidenfrost effect can be suppressed due to the acoustic radiation pressure exerted on the liquid-vapor interface; strong capillary waves are observed at the liquid-vapor interface and subsequently leads to contact between the liquid and the heated substrate. Using this technique, with 10(5)Hz vibration frequency and 10(-6)m displacement amplitude of the acoustic transducer, a maximum of 45% reduction of the initial temperature (T0â¼200-300°C) of the heated substrate can be achieved with a single droplet of volume 10(-5)l.
The unique characteristic of fast water permeation in laminated graphene oxide (GO) sheets has facilitated the development of ultrathin and ultrafast nanofiltration membranes. Here we report the application of fast water permeation property of immersed GO deposition for enhancing the performance of a GO/water nanofluid charged two-phase closed thermosyphon (TPCT). By benchmarking its performance against a silver oxide/water nanofluid charged TPCT, the enhancement of evaporation strength is found to be essentially attributed to the fast water permeation property of GO deposition instead of the enhanced surface wettability of the deposited layer. The expansion of interlayer distance between the graphitic planes of GO deposited layer enables intercalation of bilayer water for fast water permeation. The capillary force attributed to the frictionless interaction between the atomically smooth, hydrophobic carbon structures and the well-ordered hydrogen bonds of water molecules is sufficiently strong to overcome the gravitational force. As a result, a thin water film is formed on the GO deposited layers, inducing filmwise evaporation which is more effective than its interfacial counterpart, appreciably enhanced the overall performance of TPCT. This study paves the way for a promising start of employing the fast water permeation property of GO in thermal applications.
The ability to suppress the Leidenfrost effect is of significant importance in applications that require rapid and efficient cooling of surfaces with temperature higher than the Leidenfrost point TSL. The Leidenfrost effect will result in substantial reduction in cooling efficiency and hence there have been a few different approaches to suppress the Leidenfrost effect. The majority of these approaches relies on fabricating micro/nano-structures on heated surfaces, others rely on inducing an electric field between the droplets and the heated surfaces. In this paper, we present an approach that induces low frequency vibrations (fâ¼10(2) Hz) on a heated surface to suppress the effect. By mapping the different magnitudes of surface acceleration [greek xi with two dots above]sversus different initial surface temperatures Ts of the substrate, three regimes that represent three distinct impact dynamics are analyzed. Regime-I represents gentle film boiling ([greek xi with two dots above]sâ¼10(2) m s(-2) and Tsâ¼TSL), which is associated with the formation of thin spreading lamella around the periphery of the impinged droplet; Regime-II ([greek xi with two dots above]sâ¼10(2) m s(-2) and Ts>TSL) represents film boiling, which is associated with the rebound of the impinged droplet due to the presence of a thick vapor layer; Regime-III ([greek xi with two dots above]sâ¼10(3) m s(-2) and Tsâ¼TSL) represents contact boiling, which is associated with the ejection of tiny droplets due to the direct contact between the droplet and the heated surface. The estimated cooling enhancement for Regime-I is between 10% and 95%, Regime-II is between 5% and 15%, and Regime-III is between 95% and 105%. The improvement in cooling enhancement between Regime-I (strong Leidenfrost effect) and Regime-III (suppressed Leidenfrost effect) is more than 80%, demonstrating the effectiveness of using low frequency vibrations to suppress the Leidenfrost effect.
A surface acoustic wave-based sample delivery and ionization method that requires minimal to no sample pretreatment and that can operate under ambient conditions is described. This miniaturized technology enables real-time, rapid, and high-throughput analysis of trace compounds in complex mixtures, especially high ionic strength and viscous samples that can be challenging for conventional ionization techniques such as electrospray ionization. This technique takes advantage of high order surface acoustic wave (SAW) vibrations that both manipulate small volumes of liquid mixtures containing trace analyte compounds and seamlessly transfers analytes from the liquid sample into gas phase ions for mass spectrometry (MS) analysis. Drugs in human whole blood and plasma and heavy metals in tap water have been successfully detected at nanomolar concentrations by coupling a SAW atomization and ionization device with an inexpensive, paper-based sample delivery system and mass spectrometer. The miniaturized SAW ionization unit requires only a modest operating power of 3 to 4 W and, therefore, provides a viable and efficient ionization platform for the real-time analysis of a wide range of compounds.
We exploit large accelerations associated with surface acoustic waves to drive an extraordinary fluid jetting phenomena. Laterally focusing the acoustic energy to a small region beneath a drop placed on the surface causes rapid interfacial destabilization. Above a critical Weber number We, an elongated jet forms for drops with dimensions greater than the fluid sound wavelength. Further increases in We lead to single droplet pinch-off and subsequent axisymmetric breakup to form multiple droplets. A simple equation based on a momentum balance is derived to predict the jet velocity.
The ability to detect microbes, pollens and other microparticles is a critically important ability given the increasing risk of bioterrorism and emergence of antibiotic-resistant bacteria. The efficient collection of microparticles via a liquid water droplet moved by a surface acoustic wave (SAW) device is demonstrated in this study. A fluidic track patterned on the SAW device directs the water droplet's motion, and fluid streaming induced inside the droplet as it moves along is a key advantage over other particle collection approaches, because it enhances microparticle collection and concentration. Test particles consisted of 2, 10, 12 and 45 microm diameter monodisperse polystyrene and melamine microparticles; pollen from the Populus deltoides, Kochia scoparia, Secale cerale, and Broussonetia papyrifera (Paper Mulberry) species; and Escherichia coli bacteria. The collection efficiency for the synthetic particles ranged from 16 to 55%, depending on the particle size and surface tension of the collection fluid. The method was more effective in collecting pollen and the bacteria with an efficiency of 45-68% and 61.0-69.8%, respectively. Pollen collection was strongly influenced by its diameter, size, and surface geometry in a manner contrary to initial expectations. Reasons for the consistent yet unexpected collection results include leaky SAW pressure boundary segregation and shear-induced concentration of larger particles, and the subtle effects of wetting interactions. These results demonstrate a new method for collecting microparticles requiring only about one second per run, and illustrate the inadequacy of using synthetic microparticles as a substitute for their biological counterparts in experiments studying particle collection and behavior.
Microfluidic Analytical Techniques/instrumentation , Microfluidic Analytical Techniques/methods , Acoustics , Magnoliopsida , Microscopy, Electron, Scanning , Miniaturization , Particle Size , Pollen/ultrastructure , Probability , Surface Properties
