RESUMEN
Plant stems comprise nodes and internodes that specialize in solute exchange and elongation. However, their boundaries are not well defined, and how these basic units arise remains elusive. In rice with clear nodes and internodes, we found that one subclade of class I knotted1-like homeobox (KNOX1) genes for shoot meristem indeterminacy restricts node differentiation and allows internode formation by repressing YABBY genes for leaf development and genes from another node-specific KNOX1 subclade. YABBYs promote nodal vascular differentiation and limit stem elongation. YABBY and node-specific KNOX1 genes specify the pulvinus, which further elaborates the nodal structure for gravitropism. Notably, this KNOX1 subclade organization is specific to seed plants. We propose that nodes and internodes are distinct domains specified by YABBY-KNOX1 cross-regulation that diverged in early seed plants.
Asunto(s)
Regulación de la Expresión Génica de las Plantas , Proteínas de Homeodominio , Meristema , Oryza , Proteínas de Plantas , Tallos de la Planta , Proteínas de Homeodominio/genética , Proteínas de Homeodominio/metabolismo , Tallos de la Planta/anatomía & histología , Tallos de la Planta/genética , Tallos de la Planta/crecimiento & desarrollo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Meristema/genética , Meristema/crecimiento & desarrollo , Oryza/genética , Oryza/crecimiento & desarrollo , Gravitropismo/genética , Hojas de la Planta/genética , Hojas de la Planta/anatomía & histología , Hojas de la Planta/crecimiento & desarrollo , Genes de PlantasRESUMEN
The shoot and root system architectures are fundamental for crop productivity. During the history of artificial selection of domestication and post-domestication breeding, the architecture of rice has significantly changed from its wild ancestor to fulfil requirements in agriculture. We review the recent studies on developmental biology in rice by focusing on components determining rice plant architecture; shoot meristems, leaves, tillers, stems, inflorescences and roots. We also highlight natural variations that affected these structures and were utilized in cultivars. Importantly, many core regulators identified from developmental mutants have been utilized in breeding as weak alleles moderately affecting these architectures. Given a surge of functional genomics and genome editing, the genetic mechanisms underlying the rice plant architecture discussed here will provide a theoretical basis to push breeding further forward not only in rice but also in other crops and their wild relatives.
RESUMEN
Root hairs play vital roles in plant growth since they enable the efficient absorption of water and nutrients from the soil. Recent advances in Arabidopsis research have provided a deeper understanding of the molecular genetic mechanisms underlying root hair differentiation. CAPRICE (CPC) and its four homologs, which belong to the CPC gene family and encode R3 MYB transcription factors, play central roles in root hair differentiation. In this study, to better understand the functional specificity and contribution of these five CPC family genes, we conducted phenotypic and expression analyses of the CPC family proteins in a cpc mutant background. As a result, ENHANCER OF TRY AND CPC1 (ETC1) and ETC3 were found to complement the hairless root phenotype of the cpc mutant, as did CPC, whereas TRIPTYCHON (TRY) and ETC2 did not rescue the cpc phenotype. Protein expression analysis revealed that GFP fluorescence was nearly undetectable in pCPC::TRY:GFP/cpc and pCPC::ETC2:GFP/cpc plants, supporting the incapability of root hair formation in these plants. Interestingly, the fluorescence intensity of the CPC:GFP fusion protein was weaker than that of ETC1:GFP and ETC3:GFP fusion proteins. These results were inconsistent with the result of the phenotypic analysis, in which the three genes promoted root hair formation to almost the same degree in the cpc mutant background. We further discuss the discrepancy between the root hair phenotypes and the expression levels of CPC family proteins.
RESUMEN
D-type cyclins (CYCDs) are involved in a wide range of biological processes, as one of the major regulators of cell cycle activity. In Arabidopsis (Arabidopsis thaliana), three members of CYCD3 subgroup genes play important roles in plant development such as leaf development and branch formation. In rice (Oryza sativa), there is only one gene (OsCYCD3;1) belonging to the CYCD3 subgroup; its function is unknown. In this study, in order to elucidate the function of OsCYCD3;1, we generated knockout mutants of the gene and conducted developmental analysis. The knockout mutants showed a significantly reduced number of branches compared with a wild type, suggesting that OsCYCD3;1 promotes branch formation. Histological analysis showed that the activities of the axillary meristem and the shoot apical meristem (SAM) were compromised in these mutant plants. Our results suggest that OsCYCD3;1 promotes branch formation, probably by regulating cell division to maintain the activities of the axillary meristem and the SAM.
RESUMEN
Plant development depends on the activity of pluripotent stem cells in meristems, such as the shoot apical meristem and the flower meristem. In Arabidopsis thaliana, WUSCHEL (WUS) is essential for stem cell homeostasis in meristems and integument differentiation in ovule development. In rice (Oryza sativa), the WUS ortholog TILLERS ABSENT 1 (TAB1) promotes stem cell fate in axillary meristem development, but its function is unrelated to shoot apical meristem maintenance in vegetative development. In this study, we examined the role of TAB1 in flower development. The ovule, which originates directly from the flower meristem, failed to differentiate in tab1 mutants, suggesting that TAB1 is required for ovule formation. Expression of a stem cell marker was completely absent in the flower meristem at the ovule initiation stage, indicating that TAB1 is essential for stem cell maintenance in the 'final' flower meristem. The ovule defect in tab1 was partially rescued by floral organ number 2 mutation, which causes overproliferation of stem cells. Collectively, it is likely that TAB1 promotes ovule formation by maintaining stem cells at a later stage of flower development.
Asunto(s)
Diferenciación Celular/genética , Flores/genética , Oryza/genética , Proteínas de Plantas/genética , Flores/crecimiento & desarrollo , Regulación de la Expresión Génica de las Plantas/genética , Meristema/genética , Meristema/crecimiento & desarrollo , Mutación/genética , Oryza/crecimiento & desarrollo , Óvulo Vegetal/genética , Óvulo Vegetal/crecimiento & desarrollo , Desarrollo de la Planta/genética , Células Madre/citologíaRESUMEN
Tea is one of the most consumed beverages worldwide, and trichome formation in tea plant leaves impairs their commercial value. In Arabidopsis thaliana leaves, trichome formation is negatively regulated by the CPC family genes, which encode R3-type MYB transcription factors. Here, we identified six CPC-like genes in a tea plant (Camellia sinensis var. sinensis) for the first time. Simulated three-dimensional structure of the MYB domains of all the six CPC-like proteins exhibited negative charge on the surface, as observed on that of the Arabidopsis CPC protein that does not bind to DNA, indicating their similarity with regard to molecular interaction. We further found that the six CPC-like genes were differentially expressed in different developmental stages of tea leaves, and four out of the six genes were upregulated in the youngest 1st leaves, which formed more trichomes than other older leaves. Although it does not establish a causal link, the correlation between differential expression of CPC-like genes and variable trichome formation suggests that the R3-type MYB transcription factors are potential precipitating factors in affecting the value of tea leaf.
Asunto(s)
Camellia sinensis/genética , Camellia sinensis/fisiología , Genes de Plantas , Hojas de la Planta/genética , Hojas de la Planta/fisiología , Proteínas Proto-Oncogénicas c-myb/genética , Tricomas/genética , Tricomas/fisiología , Productos Agrícolas/genética , Productos Agrícolas/fisiología , Regulación de la Expresión Génica de las Plantas , Variación Genética , Japón , Proteínas Proto-Oncogénicas c-myb/fisiologíaRESUMEN
TRIPTYCHON (TRY) is one of the R3-MYB transcription factors. Its extended C-terminal 19 amino-acid region (CTRY) is considered to affect the ability of root hair differentiation in Arabidopsis. Here, to further understand the function of CTRY, it, together with GFP, was artificially fused with TRY homologs, CPC and ETC1, which do not contain such extended regions and induce root hair differentiation. Arabidopsis transgenic plants carrying the fusion proteins, CPC-CTRY-GFP and ETC1-CTRY-GFP, induced root hair differentiation as observed in those carrying the original proteins without CTRY. The expression levels of the fusion proteins in the transgenic plants were essentially the same as those of the original proteins, although their subcellular localization to nuclei of root epidermal cells was slightly changed by CTRY. Therefore, CTRY does not affect the ability of CPC and ETC1 to induce root hair differentiation when artificially fused, and its function may be restricted in TRY.
Asunto(s)
Proteínas de Arabidopsis/genética , Arabidopsis/genética , Proteínas de Unión al ADN/genética , Epidermis de la Planta/genética , Raíces de Plantas/genética , Proteínas Proto-Oncogénicas c-myb/genética , Factores de Transcripción/genética , Secuencia de Aminoácidos , Arabidopsis/citología , Arabidopsis/metabolismo , Proteínas de Arabidopsis/metabolismo , Diferenciación Celular , Proteínas de Unión al ADN/metabolismo , Regulación de la Expresión Génica de las Plantas , Proteínas Fluorescentes Verdes/genética , Proteínas Fluorescentes Verdes/metabolismo , Células Vegetales/metabolismo , Epidermis de la Planta/citología , Epidermis de la Planta/metabolismo , Raíces de Plantas/citología , Raíces de Plantas/metabolismo , Plantas Modificadas Genéticamente , Proteínas Proto-Oncogénicas c-myb/metabolismo , Proteínas Recombinantes de Fusión/genética , Proteínas Recombinantes de Fusión/metabolismo , Alineación de Secuencia , Homología de Secuencia de Aminoácido , Factores de Transcripción/metabolismoRESUMEN
The Elongator complex, which is conserved in eukaryotes, has multiple roles in diverse organisms. In Arabidopsis thaliana, Elongator is shown to be involved in development, hormone action and environmental responses. However, except for Arabidopsis, our knowledge of its function is poor in plants. In this study, we initially carried out a genetic analysis to characterize a rice mutant with narrow and curled leaves, termed curled later1 (cur1). The cur1 mutant displayed a heteroblastic change, whereby the mutant leaf phenotype appeared specifically at a later adult phase of vegetative development. The shoot apical meristem (SAM) was small and the leaf initiation rate was low, suggesting that the activity of the SAM seemed to be partially reduced in cur1. We then revealed that CUR1 encodes a yeast ELP1-like protein, the largest subunit of Elongator. Furthermore, disruption of OsELP3 encoding the catalytic subunit of Elongator resulted in phenotypes similar to those of cur1, including the timing of the appearance of mutant phenotypes. Thus, Elongator activity seems to be specifically required for leaf development at the late vegetative phase. Transcriptome analysis showed that genes involved in protein quality control were highly upregulated in the cur1 shoot apex at the later vegetative phase, suggesting the restoration of impaired proteins probably produced by partial defects in translational control due to the loss of function of Elongator. The differences in the mutant phenotype and gene expression profile between CUR1 and its Arabidopsis ortholog suggest that Elongator has evolved to play a unique role in rice development.
Asunto(s)
Meristema/fisiología , Oryza/fisiología , Hojas de la Planta/crecimiento & desarrollo , Proteínas de Plantas/metabolismo , Perfilación de la Expresión Génica , Regulación de la Expresión Génica de las Plantas , Técnicas de Inactivación de Genes , Histona Acetiltransferasas/genética , Complejos Multiproteicos , Mutación , Oryza/crecimiento & desarrollo , Factores de Elongación de Péptidos/genética , Fenotipo , Hojas de la Planta/citología , Hojas de la Planta/metabolismo , Proteínas de Plantas/genética , Plantas Modificadas Genéticamente , Subunidades de Proteína , Proteínas de Saccharomyces cerevisiae/genéticaRESUMEN
In plants, reversible histone acetylation and deacetylation play a crucial role in various biological activities, including development and the response to environmental stress. Histone deacetylation, which is generally associated with gene silencing, is catalyzed by multiple histone deacetylases (HDACs). Our understanding of HDAC function in plant development has accumulated from molecular genetic studies in Arabidopsis thaliana. By contrast, how HDACs contribute to the development of rice (Oryza sativa) is poorly understood and no rice mutants of HDAC have been reported. Here we have characterized a new rice mutant showing semi-dwarfism, which we named dwarf with slender leaf1 (dsl1). The mutant showed pleiotropic defects in both vegetative and reproductive developments; e.g. dsl1 produced short and narrow leaves, accompanied by a reduction in the number and size of vascular bundles. The semi-dwarf phenotype was due to suppression of the elongation of some culm (stem) internodes. Interestingly, despite this suppression of the upper internodes, the elongation and generation of lower internodes were slightly enhanced. Inflorescence and spikelet development were also affected by the dsl1 mutation. Some of the observed morphological defects were related to a reduction in cell numbers, in addition to reduced cell division in leaf primordia revealed by in situ hybridization analysis, suggesting the possibility that DSL1 is involved in cell division control. Gene cloning revealed that DSL1 encodes an HDAC belonging to the reduced potassium dependence3/histone deacetylase1 family. Collectively, our study shows that the HDAC DSL1 plays diverse and important roles in development in rice.
Asunto(s)
Sistema Enzimático del Citocromo P-450/metabolismo , Histona Desacetilasas/genética , Histona Desacetilasas/metabolismo , Oryza/crecimiento & desarrollo , Oryza/metabolismo , División Celular , Clonación Molecular , Genes de Plantas , Histonas/metabolismo , Mutación , Oryza/genética , Fenotipo , Hojas de la Planta/citología , Hojas de la Planta/crecimiento & desarrollo , Proteínas de Plantas/metabolismo , Brotes de la Planta/citología , Brotes de la Planta/crecimiento & desarrolloRESUMEN
Shoot branches are formed from the axillary meristem and their formation is a key process in plant development. Although our understanding of the mechanisms underlying stem cell maintenance in the shoot apical meristem (SAM) is progressing, our knowledge of these mechanisms during the process of axillary meristem development is insufficient. To elucidate the genetic mechanisms underlying axillary meristem development in rice (Oryza sativa), we undertook a molecular genetic analysis focusing on TILLERS ABSENT1 (TAB1) and FLORAL ORGAN NUMBER2 (FON2), respective orthologs of the WUSCHEL and CLAVATA3 genes involved in SAM maintenance in Arabidopsis (Arabidopsis thaliana). We revealed that stem cells were established at an early stage of axillary meristem development in the wild-type, but were not maintained in tab1. By contrast, the stem cell region and TAB1 expression domain were expanded in fon2, and FON2 overexpression inhibited axillary meristem formation. These results indicate that TAB1 is required to maintain stem cells during axillary meristem development, whereas FON2 negatively regulates stem cell fate by restricting TAB1 expression. Thus, the genetic pathway regulating SAM maintenance in Arabidopsis seems to have been recruited to play a specific role within a narrow developmental window - namely, axillary meristem establishment - in rice.
Asunto(s)
Meristema/citología , Meristema/crecimiento & desarrollo , Oryza/citología , Oryza/crecimiento & desarrollo , Proteínas de Plantas/metabolismo , Células Madre/citología , Mutación/genética , Oryza/anatomía & histología , Fenotipo , Células Madre/metabolismo , Supresión GenéticaRESUMEN
Stem cell maintenance in the shoot apical meristem (SAM) is very important for plant development and is regulated by the WUSCHEL-CLAVATA (WUS-CLV) feedback loop in Arabidopsis (Arabidopsis thaliana). WUS promotes stem cell identity, whereas CLV negatively regulates stem cell proliferation by repressing WUS expression. We previously showed that, in rice (Oryza sativa), the WUS ortholog TILLERS ABSENT1 (TAB1, also known as OsWUS) has no function in SAM maintenance, whereas it plays a crucial role in axillary meristem development. Recently, we showed that a double mutant of FLORAL ORGAN NUMBER2 (FON2) and ABERRANT SPIKELET AND PANICLE1 (ASP1) led to a marked enlargement of the inflorescence meristem, and that the TAB1 function is not associated with massive stem cells in this meristem. In this paper, we confirmed that TAB1 is also unrelated to the enlargement of the SAM in the vegetative phase of the fon2 and fon2 asp1 mutants. In addition, misexpression of TAB1 under the promoter of FON1 led to a slight reduction of the SAM size in wild type, suggesting that TAB1 is not a positive regulator of stem cells. Taking together, TAB1 seems not to be involved in meristem maintenance, irrespective of the meristem type.
Asunto(s)
Proteínas de Arabidopsis/química , Proteínas de Homeodominio/química , Meristema/citología , Meristema/metabolismo , Oryza/metabolismo , Proteínas de Plantas/metabolismo , Homología de Secuencia de Aminoácido , Células Madre/metabolismo , Mutación/genética , Tamaño de los Órganos , Oryza/anatomía & histología , Proteínas de Plantas/genéticaRESUMEN
Stem cell homeostasis is maintained by the WUSCHEL-CLAVATA (WUS-CLV) negative feedback loop in Arabidopsis (Arabidopsis thaliana). In rice (Oryza sativa), FLORAL ORGAN NUMBER2 (FON2) functions in the negative regulation of stem cell proliferation, similar to Arabidopsis CLV3 In this study, through genetic enhancer analysis, we found that loss of function of ABERRANT SPIKELET AND PANICLE1 (ASP1), encoding an Arabidopsis TOPLESS (TPL)-like transcriptional corepressor, enhances the fon2 flower phenotype, which displayed an increase in floral organ number. In the fon2 asp1 double mutant, the inflorescence was severely affected, resulting in bifurcation of the main axis (rachis), a phenotype that has not previously been reported. The stem cells showed marked overproliferation in fon2 asp1, resulting in extreme enlargement and splitting of the inflorescence meristem. These results suggest that ASP1 and FON2 synergistically regulate stem cell maintenance in rice. Unexpectedly, genetic analysis indicated that TILLERS ABSENT1, the rice ortholog of WUS, is not involved in promoting stem cell proliferation in this meristem. Transcriptome analysis suggested that ASP1 and FON signaling negatively regulate a set of genes with similar functions, and they act on these genes in concert. Taken together, our results suggest that TPL-like corepressor activity plays a crucial role in meristem maintenance, and that stem cell proliferation is properly maintained via the cooperation of ASP1 and FON2.
Asunto(s)
Proteínas Co-Represoras/genética , Flores/genética , Regulación de la Expresión Génica de las Plantas , Meristema/genética , Oryza/genética , Proteínas de Plantas/genética , Transducción de Señal/genética , Proliferación Celular/genética , Flores/citología , Flores/ultraestructura , Perfilación de la Expresión Génica/métodos , Ontología de Genes , Meristema/citología , Meristema/ultraestructura , Microscopía Electrónica de Rastreo , Mutación , Oryza/citología , Plantas Modificadas Genéticamente , Células Madre/citología , Células Madre/metabolismoRESUMEN
The ABC model in flower development represents a milestone of plant developmental studies and is essentially conserved across a wide range of angiosperm species. Despite this overall conservation, individual genes in the ABC model are not necessarily conserved and sometimes play a species-specific role, depending on the plant. We previously reported that carpels are specified by the YABBY gene DROOPING LEAF (DL) in rice (Oryza sativa), which bears flowers that are distinct from those of eudicots. In contrast, another group reported that carpels are specified by two class C genes, OsMADS3 and OsMADS58. Here, we have addressed this controversial issue by phenotypic characterization of floral homeotic gene mutants. Analysis of a complete loss-of-function mutant of OsMADS3 and OsMADS58 revealed that carpel-like organs expressing DL were formed in the absence of the two class C genes. Furthermore, no known flower organs including carpels were specified in a double mutant of DL and SUPERWOMAN1 (a class B gene), which expresses only class C genes in whorls 3 and 4. These results suggest that, in contrast to Arabidopsis, class C genes are not a key regulator for carpel specification in rice. Instead, they seem to be involved in the elaboration of carpel morphology rather than its specification. Our phenotypic analysis also revealed that, similar to its Arabidopsis ortholog CRABS CLAW, DL plays an important function in regulating flower meristem determinacy in addition to carpel specification.
Asunto(s)
Flores/crecimiento & desarrollo , Meristema/crecimiento & desarrollo , Oryza/crecimiento & desarrollo , Flores/ultraestructura , Regulación del Desarrollo de la Expresión Génica , Regulación de la Expresión Génica de las Plantas , Genes de Plantas/fisiología , Microscopía Electrónica de Rastreo , Oryza/genética , Oryza/ultraestructura , Proteínas de Plantas/genética , Proteínas de Plantas/fisiologíaRESUMEN
Inflorescence architecture is diverse in angiosperms, and is mainly determined by the arrangement of the branches and flowers, known as phyllotaxy. In rice (Oryza sativa), the main inflorescence axis, called the rachis, generates primary branches in a spiral phyllotaxy, and flowers (spikelets) are formed on these branches. Here, we have studied a classical mutant, named verticillate rachis (ri), which produces branches in a partially whorled phyllotaxy. Gene isolation revealed that RI encodes a BELL1-type homeodomain transcription factor, similar to Arabidopsis PENNYWISE/BELLRINGER/REPLUMLESS, and is expressed in the specific regions within the inflorescence and branch meristems where their descendant meristems would soon initiate. Genetic combination of an ri homozygote and a mutant allele of RI-LIKE1 (RIL1) (designated ri ril1/+ plant), a close paralog of RI, enhanced the ri inflorescence phenotype, including the abnormalities in branch phyllotaxy and rachis internode patterning. During early inflorescence development, the timing and arrangement of primary branch meristem (pBM) initiation were disturbed in both ri and ri ril1/+ plants. These findings suggest that RI and RIL1 were involved in regulating the phyllotactic pattern of the pBMs to form normal inflorescences. In addition, both RI and RIL1 seem to be involved in meristem maintenance, because the ri ril1 double-mutant failed to establish or maintain the shoot apical meristem during embryogenesis.
Asunto(s)
Inflorescencia/embriología , Inflorescencia/metabolismo , Meristema/embriología , Meristema/metabolismo , Oryza/embriología , Oryza/metabolismo , Proteínas de Plantas/metabolismo , Regulación de la Expresión Génica de las Plantas , Inflorescencia/genética , Meristema/genética , Oryza/genética , Proteínas de Plantas/genéticaRESUMEN
YABBY genes play important roles in the development of lateral organs such as leaves and floral organs in Angiosperms. However, the function of YABBY genes is poorly understood in monocots. We focused on three rice (Oryza sativa) YABBY genes, TONGARI-BOUSHI (TOB1, TOB2, TOB3), which are closely related to Arabidopsis (Arabidopsis thaliana) FILAMENTOUS FLOWER (FIL). To elucidate the function of these YABBY genes, we employed a reverse genetic approach. TOB genes were expressed in bract and lateral organ primordia, but not in meristems. RNAi knockdown of TOB2 or TOB3 in the tob1 mutant caused abnormal spikelet development. Furthermore, simultaneous knockdown of both TOB2 and TOB3 in tob1 affected not only spikelet, but also inflorescence development. In severe cases, the inflorescences comprised naked branches without spikelets. Analysis of inflorescence development at an early stage showed that the observed phenotypic defects were closely associated with a failure to initiate and maintain reproductive meristems. These results indicate that the TOB genes regulate the maintenance and fate of all reproductive meristems. It is likely that the function of FIL/TOB clade YABBY genes has been conserved between Arabidopsis and rice to maintain the proper function of meristems, even though these genes are expressed in lateral organ primordia.
Asunto(s)
Meristema/fisiología , Oryza/fisiología , Proteínas de Plantas/genética , Copas de Floración/genética , Regulación de la Expresión Génica de las Plantas , Inflorescencia/genética , Inflorescencia/fisiología , Meristema/genética , Mutación , Oryza/genética , Proteínas de Plantas/metabolismo , Plantas Modificadas Genéticamente , Interferencia de ARNRESUMEN
Meristems such as the shoot apical meristem and flower meristem (FM) act as a reservoir of stem cells, which reproduce themselves and supply daughter cells for the differentiation of lateral organs. In Oryza sativa (rice), the FLORAL ORGAN NUMBER2 (FON2) gene, which is similar to Arabidopsis CLAVATA3, is involved in meristem maintenance. In fon2 mutants, the numbers of floral organs are increased due to an enlargement of the FM. To identify new factors regulating meristem maintenance in rice, we performed a genetic screening of mutants that enhanced the fon2 mutation, and found a mutant line (2B-424) in which pistil number was dramatically increased. By using a map-based approach and next-generation sequencing, we found that the line 2B-424 had a complete loss-of-function mutation (a large deletion) in OsMADS3, a class C MADS-box gene that is known to be involved in stamen specification. Disruption of OsMADS3 in the fon2 mutant by CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats-CRISPR-associated protein 9) technology caused a flower phenotype similar to that of 2B-424, confirming that the gene responsible for enhancement of fon2 was OsMADS3. Morphological analysis showed that the fon2 and osmads3 mutations synergistically affected pistil development and FM determinacy. We also found that whorl 3 was duplicated in mature flowers and the FM was enlarged at an early developmental stage in severe osmads3 single mutants. These findings suggest that OsMADS3 is involved not only in FM determinacy in late flower development but also in FM activity in early flower development.
Asunto(s)
Flores/citología , Flores/metabolismo , Meristema/citología , Meristema/metabolismo , Oryza/citología , Oryza/metabolismo , Proteínas de Plantas/metabolismo , Flores/genética , Regulación de la Expresión Génica de las Plantas/genética , Regulación de la Expresión Génica de las Plantas/fisiología , Proteínas de Dominio MADS/genética , Proteínas de Dominio MADS/metabolismo , Meristema/genética , Mutación , Oryza/genética , Proteínas de Plantas/genética , Plantas Modificadas Genéticamente/citología , Plantas Modificadas Genéticamente/genética , Plantas Modificadas Genéticamente/metabolismoRESUMEN
Angiosperms produce diverse flowers and the pattern of floral symmetry is a major factor for flower diversification. Bilaterally symmetric flowers have evolved multiple times in different angiosperm lineages from radially symmetric ancestors. Whereas most monocots produce radially symmetric flowers, grasses such as rice (Oryza sativa) and maize (Zea mays) generate bilaterally symmetric flowers and spikelets. In this paper, we focused on the two opposite lemma (tol) mutant, which displays a pleiotropic phenotype in the spikelet. Close morphological examination revealed that a typical spikelet phenotype of the tol mutant was principally based on the mirror image duplication of the lemma-side half of the spikelet. Other spikelet phenotypes can be explained as the derivation from the spikelet with this duplication. A polar pattern of organ formation along the lemma-palea axis was disrupted by this duplication. Accordingly, tol mutation seems to change the spikelet from bilateral symmetry (monosymmetry) to disymmetry. Thus, the tol mutant provides good genetic material to investigate the regulation of spikelet symmetry in rice.
Asunto(s)
Flores/anatomía & histología , Mutación , Oryza/genética , Proteínas de Plantas/genética , Tipificación del Cuerpo , Flores/genética , Regulación del Desarrollo de la Expresión Génica , Regulación de la Expresión Génica de las Plantas , Oryza/anatomía & histología , Oryza/crecimiento & desarrollo , Fenotipo , Proteínas de Plantas/metabolismoRESUMEN
CRISPR-Cas9 technology, which uses an RNA-guided nuclease, has been developed as an efficient and versatile genome-editing method to induce mutations in genes of interest. To examine the feasibility of this method in developmental studies of a model monocot, rice (Oryza sativa), we introduced the construct gDL-1, which produced a guide RNA targeting the DROOPING LEAF (DL) gene. DL regulates midrib formation in the leaf and carpel specification in the flower. Because loss of function of DL causes the drooping leaf phenotype in regenerated seedlings, the effect of gene disruption should be easily detected. In transgenic plants carrying gDL-1, the DL gene was disrupted at high efficiency: seven out of nine plants examined had bi-allelic mutations. All transgenic plants with the bi-allelic mutation showed the drooping leaf phenotype. Observation of cross sections of the leaf blade clearly indicated that these transgenic plants failed to make midrib structures, and were comparable to the severe dl mutant dl-sup1. Thus, CRISPR-Cas9 technology can be a useful and efficient tool in developmental studies in rice.
Asunto(s)
Oryza/genética , Hojas de la Planta/anatomía & histología , Proteínas de Plantas/genética , Sistemas CRISPR-Cas , Regulación del Desarrollo de la Expresión Génica , Regulación de la Expresión Génica de las Plantas , Mutación , Hojas de la Planta/genética , Plantas Modificadas Genéticamente/genéticaRESUMEN
In rice (Oryza sativa), floral organs develop in the spikelet, an inflorescence unit unique to grass species. The floral organs, such as carpels, stamens and lodicules, are enclosed by two spikelet organs, the palea and lemma. The number of floral organs is genetically regulated. Mutations in the FLORAL ORGAN NUMBER (FON) genes cause an increase in the number of carpels and stamens due to an enlargement of the floral meristem. The spikelet organs, such as lemma and palea, are less affected in the fon mutants. We found a mutant, fickle spikelet1 (fsp1), that displayed an increased number not only of floral organs but also of spikelet organs. Because the fsp1 spikelets showed a pleiotropic phenotype, we classified them into four types. The expressivity of the fsp1 phenotype varied from plant to plant, and also from panicle to panicle within a single plant. In addition, the frequency of each fsp1 spikelet type also varied considerably among plants and among panicles within a plant. When the fsp1 mutants were grown in a growth chamber, an extra abnormality, namely a defect in pollen development, was observed. Furthermore, the expressivity of the mutant phenotype increased dramatically in mutant plants grown in a growth chamber. Thus, the expressivity of the fsp1 phenotype seems to be strongly influenced by environmental conditions.