RESUMEN
PURPOSE: Human males absent on the first (hMOF) is a histone acetyltransferase (HAT) and is responsible for acetylating histone H4 at lysine 16 (H4K16). Recent studies have indicated that hMOF is overexpressed in non-small-cell lung cancer (NSCLC) as an oncogene. The aim of this study is to profile the prognostic roles of hMOF in patients with unresectable stage III NSCLC undergoing definitive radiotherapy (RT) and in the radiosensitivity of human NSCLC cells. MATERIALS AND METHODS: The expression of hMOF was detected in 24 normal and tumor-paired fresh-frozen NSCLC tissue samples. The immunohistochemistry was conducted, and the correlation of hMOF with clinicopathological parameters was studied in tissues from 90 patients with unresectable stage III NSCLC who underwent definitive RT. Radiation sensitivity was monitored using clonogenic assays in NCI-H1299 and A549 NSCLC cell lines with hMOF knockdown. RESULTS: hMOF was overexpressed in NSCLC tissues compared with non-cancerous tissues. Compared to patients with downregulated hMOF, upregulated hMOF was observed in 51.1% (46/90) of the patients, who showed a significantly worse 5-year survival rate (5.4% vs 22.9%, P=0.025). hMOF expression was an independent prognostic factor of unresectable stage III NSCLC patients who underwent definitive RT. Silencing hMOF increased in vitro the sensitive enhancing ratio (SER) of NSCLC cell lines and downregulated the expression of phospho-ataxia telangiectasia mutated (p-ATM) and RAD51 after irradiation (IR). CONCLUSION: Overexpression of hMOF predicts poor prognosis in patients with unresectable stage III NSCLC undergoing definitive RT. Downregulating hMOF might be a promising intervention to improve the outcome after RT.
RESUMEN
An electronic nose, core detector of which was composed of three metal-doped SnO2 gas sensors and a photo ionization detector (PID) as a sensor array, was developed for rapid detection of volatile chloralkane and chloroalkene. A gas recognition model was developed based on test and analysis with nine of pure gas and five of mixtures, and then the electronic nose was applied to several water samples and the validity was evaluated with a gas chromatography. The results revealed that the sensor array responded differently between the chloralkane and chloroalkene. PID was less sensitive to chloralkane, while linearly responded to chloroalkene (R2 > 0.997). Sensor TGS2602 performed sensitive to carbon tetrachloride (CT), trichloromethane (TCM) and 1,2-dichloroethane (1,2-DCA), with a linear response to the former two but a poor linear response to 1,2-DCA. Sensors TGS2600 and TGS2620 were by far more sensitive and linearly (R2 > 0.995) responded to dichloromethane (DCM) and 1,2-DCA. Therefore in the final gas recognition model, PID was used to determine the concentration of chloroalkene, sensor TGS2602 was used to determine CT and TCM, sensor TGS2600 or TGS2620 was used to determine DCM and 1,2-DCA. When applied to gas mixtures, sensor TGS2602 responded less sensitive than the sum of the response to each single component, while other sensors responded equally. The electronic nose showed a determined result linearly correlated to GC (R2 > 0.96) as applied to samples with a mixture of DCM and perchloroethylene.