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Anthelmintic resistance in livestock animals has been spreading across the world in prevalence and severity. As a result, researchers are exploring alternative strategies to combat this issue, and one promising avenue is the utilization of medicinal plants. This study aims to investigate the anthelmintic efficacy of the crude ethanolic extract (CEE) derived from the leaves of Juglans regia against one of the most detrimental nematode parasites affecting poultry, namely Ascaridia galli (A. galli). For the in vitro studies, adult A. galli worms were collected from the naturally infected chickens and the efficacy of CEE was measured at the concentration of 25, 50, and 100 mg/ml using adult worm motility inhibition (WMI) assay. In addition, levamisole (0.55 mg/ml) was used as the positive control. Likewise, Phosphate buffered saline (PBS) was used as the negative control. For the in vivo studies, CEE of J.regia at the doses of 500, 1000, and 2000 mg/kg were evaluated in chickens experimentally infected with A. galli. The anthelmintic efficacy was monitored using faecal egg count reduction (FECR) and worm count reduction (WCR) assays. In vitro studies revealed significant (P < 0.001) anthelmintic effects of CEE of J.regia on the motility of A. galli worms at different hours post-exposure. At the concentration of 100 mg/ml, CEE resulted in 96.5% inhibition of worm motility at 24 h post-exposure. While the synthetic anthelmintic drug, levamisole caused the highest inhibition of worm motility (100%) at the same time period. The in vivo anthelmintic activity of CEE of J. regia demonstrated a maximum effect on day 14 post-treatment by inducing 67.28% FECR and 65.03% WCR. We observed no significant difference (P > 0.05) in worm counts between the negative control group and the chickens treated with CEE at the dosage of 500 mg/kg. Together, the results of the present study suggest that CEE of J. regia leaves possess anthelmintic properties and could be a potential source of novel anthelmintic compounds for controlling helminth parasites.
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Antihelmínticos , Ascaridia , Ascaridiasis , Pollos , Juglans , Extractos Vegetales , Hojas de la Planta , Enfermedades de las Aves de Corral , Animales , Ascaridia/efectos de los fármacos , Extractos Vegetales/farmacología , Hojas de la Planta/química , Juglans/química , Enfermedades de las Aves de Corral/tratamiento farmacológico , Enfermedades de las Aves de Corral/parasitología , Antihelmínticos/farmacología , Ascaridiasis/veterinaria , Ascaridiasis/tratamiento farmacológico , Ascaridiasis/parasitología , Etanol/química , Etanol/farmacologíaRESUMEN
AIM OF THE STUDY: The growing resistance of helminth parasites to currently available commercial anthelmintic drugs, combined with apprehensions regarding detrimental chemical residues in livestock products, has sparked an interest in exploring medicinal plants as an alternative strategy for treating helminthiasis. As a result, this study was designed to investigate the anthelmintic activity of crude methanolic extracts (CME) of Saussurea costus root on Ascaridia.galli, a pathogenic nematode of poultry. MATERIALS AND METHODS: In vitro, the anthelmintic effect of Saussurea costus root was evaluated in comparison to commercial anthelmintic, levamisole on the adult nematode parasites, A.galli using worm motility inhibition (WMI) test. The CME of S.costus was also evaluated for in vivo anthelmintic activity in chickens experimentally infected with Ascaridia galli. For the in vivo study, one hundred-day-old chickens were orally infected with embryonated eggs of A. galli worms. The efficacy of the plant extract as an anthelmintic was assessed through two tests: faecal egg count reduction (FECR) test and worm count reduction (WCR) test. The study investigated three distinct doses of plant extract under in vivo setup: 500 mg kg-1 body weight (bw), 1000 mg kg-1 bw, and 2000 mg kg-1 bw. RESULTS: In vitro, all the tested concentrations of S.costus (25 mg/ml, 50 mg/ml, and 100 mg/ml) showed a significant (P < 0.001) anthelmintic effects on live adult A. galli worms in terms of inhibition of worm motility at different hours post-treatment. At the highest concentration of the extract, we observed worm motility inhibition of 100% at 24 h post-exposure. On day 14 post-treatment, all birds were slaughtered, and adult A. galli worms were subsequently retrieved from their small intestines. Birds treated with CME extract of S. costus root exhibited a significant (P < 0.001) reduction in faecal egg count. However, the administration of the extract at the dosage of 500 mg kg-1bw to the birds did not reveal any significant (P > 0.05) differences in the worm count compared to the negative control group. The CME of S. costus at a dose of 2000 mg kg-1bw showed the highest anthelmintic activity by inducing 83.10% FECR and 76.47% WCR. CONCLUSION: In conclusion, the root extract of S. costus has a promising anthelmintic activity on A. galli as demonstrated by the results of the present experiment.
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Antihelmínticos , Ascaridia , Ascaridiasis , Pollos , Extractos Vegetales , Enfermedades de las Aves de Corral , Saussurea , Animales , Ascaridia/efectos de los fármacos , Extractos Vegetales/farmacología , Extractos Vegetales/química , Enfermedades de las Aves de Corral/parasitología , Enfermedades de las Aves de Corral/tratamiento farmacológico , Antihelmínticos/farmacología , Pollos/parasitología , Saussurea/química , Ascaridiasis/veterinaria , Ascaridiasis/tratamiento farmacológico , Ascaridiasis/parasitología , Recuento de Huevos de Parásitos , Heces/parasitología , Raíces de Plantas/química , Levamisol/farmacología , Levamisol/uso terapéuticoRESUMEN
We sought to study the feasibility of axillary artery as alternative access for mechanical circulatory support (MCS) in cardiogenic shock and high-risk percutaneous coronary intervention (HR-PCI) patients with severe occlusive peripheral artery disease (PAD). In patients with severe PAD, the iliofemoral artery may be so diseased preventing deployment of MCS, precluding the use of lifesaving therapy. In such circumstances, the axillary artery may be a viable access site. Records of all patients presenting with cardiogenic shock or HR-PCI requiring MCS through axillary artery access at our institution from January 2016 to September 2018 were examined. Demographics, clinical, procedural, and outcomes data were collected on all patients. A total of 48 patients presented with cardiogenic shock (60%) or HR-PCI (40%) requiring MCS via axillary artery due to prohibitive PAD (mean age 66 ± 11 years). Admission diagnoses were non-ST segment elevation myocardial infarction (38%), unstable angina (23%), ST segment elevation myocardial infarction (19%), and cardiac arrest (21%). Time from axillary access to activation of Impella was 11.9 ± 4 minutes. Four patients required concomitant Impella RP for right ventricular support due to biventricular cardiogenic shock. Twenty-two patients died before Impella was explanted due to multiorgan failure, stroke, and infection. None of the patients who died had vascular complications related to axillary access. Axillary artery appears to be a viable alternative access for large bore devices in patients with prohibitive PAD. As experience of the field with this approach grows, it may be the default access for deployment of large bore sheaths in the future.
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Angina Inestable/terapia , Arteria Axilar , Paro Cardíaco/terapia , Corazón Auxiliar , Infarto del Miocardio sin Elevación del ST/terapia , Intervención Coronaria Percutánea/métodos , Infarto del Miocardio con Elevación del ST/terapia , Choque Cardiogénico/terapia , Adulto , Anciano , Anciano de 80 o más Años , Angina Inestable/complicaciones , Estudios de Factibilidad , Femenino , Arteria Femoral , Paro Cardíaco/complicaciones , Humanos , Arteria Ilíaca , Masculino , Persona de Mediana Edad , Infarto del Miocardio sin Elevación del ST/complicaciones , Enfermedad Arterial Periférica/complicaciones , Estudios Retrospectivos , Infarto del Miocardio con Elevación del ST/complicaciones , Choque Cardiogénico/etiología , Volumen Sistólico , Adulto JovenRESUMEN
In the interests of food safety and public health, plants and their compounds are now re-emerging as an alternative approach to treat parasitic diseases. Here, we studied the anticoccidial effect of different solvent extracts of the fruit peel of Punica granatum-a commercial waste from pomegranate juice industries. The hope underlying these experiments was to find a sustainable natural product for controlling coccidiosis. The plant extracts were prepared using solvents of different polarity. Acute oral toxicity study was first carried out to see the safety of crude extracts. A high dose of crude extracts (300â¯mg/kg body weight) was tested for possession of anticoccidial activity against experimentally induced coccidial infection in broiler chicken. Activity was measured in comparison to the reference drug amprolium on the basis of oocyst output reduction, mean weight gain of birds and feed conversion ratio. Oocyst output was measured using Mc-Masters counting technique. Acute oral toxicity study showed that crude extracts of P. granatum are safe up to dosage of 2000â¯mg/kg body weight. LD50 was not determined as mortalities were not recorded in any of the five groups of chicken. For anticoccidial activity crude methanolic extract (CME) of the fruit peel of P. granatum showed the maximum effect as evident by oocyst output reduction (92.8⯱â¯15.3), weight gain of birds (1403.0⯱â¯11.9â¯g) and feed conversion ratio (1.66⯱â¯0.04), thereby affirming the presence of alcohol soluble active ingredients in the plant. We also tested different doses (100-400â¯mg/kg body weight) of the CME of the fruit peel of P. granatum, the most active extract on E. tenella and observed a dose dependent effect. From the present study it can be concluded that alcoholic extract of the fruit peel of P. granatum has significant potential to contribute to the control of coccidian parasites of chicken.
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Antiprotozoarios/administración & dosificación , Coccidiosis/tratamiento farmacológico , Eimeria/efectos de los fármacos , Lythraceae/química , Fitoquímicos/administración & dosificación , Extractos Vegetales/administración & dosificación , Animales , Antiprotozoarios/efectos adversos , Antiprotozoarios/aislamiento & purificación , Antiprotozoarios/farmacología , Pollos , Modelos Animales de Enfermedad , Efectos Colaterales y Reacciones Adversas Relacionados con Medicamentos , Frutas/química , Fitoquímicos/efectos adversos , Fitoquímicos/aislamiento & purificación , Fitoquímicos/farmacología , Extractos Vegetales/efectos adversos , Extractos Vegetales/aislamiento & purificación , Extractos Vegetales/farmacología , Resultado del TratamientoRESUMEN
Coccidiosis, caused by various species of genus Eimeria, is a major parasitic disease in chicken. The increasing resistance of these parasites to currently used anticoccidial drugs has stimulated the search for new methods of control. As part of this effort, a study was designed to see the anticoccidial effect of different solvent extracts of Artemisia vestita-a traditional herb growing in Kashmir Himalayas. The plant extracts were prepared using different solvents. Preliminary toxicity study was first carried out to see the safety of crude plant extracts. A high dose of crude extracts (300 mg/kg body weight) was tested for possession of anticoccidial activity against experimentally induced coccidial infection in broiler chicken. Activity was measured in comparison to the reference drug amprolium on the basis of oocyst output reduction, mean weight gain of birds and feed conversion ratio. Oocyst output was measured using Mc-Masters counting technique. Preliminary toxicity study showed that crude extracts of A. vestita are safe up to dosage of 2000 mg/kg body weight. LD50 was not determined as mortalities were not recorded in any of the five groups of chicken. For anticoccidial activity crude methanolic extract (CME) of A. vestita showed the maximum effect as evident by oocyst output reduction (71.5 ± 12.2), weight gain of birds (1406.4 ± 12.2) and feed conversion ratio (1.58 ± 0.06), thereby affirming the presence of alcohol soluble active ingredients in the plant. We also tested different doses (100-400 mg/kg body weight) of the CME of A. vestita, the most active extract on E. tenella and observed a dose dependent effect. From the present study it can be concluded that alcoholic extract of A. vestita has the immense potential to contribute to the control of coccidian parasites of chicken. Our results corroborate the use of genus Artemisia and could justify its use in folk medicine for treatment of parasitic diseases.
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Antiprotozoarios/administración & dosificación , Artemisia/química , Coccidiosis/tratamiento farmacológico , Eimeria/efectos de los fármacos , Fitoquímicos/administración & dosificación , Extractos Vegetales/administración & dosificación , Animales , Antiprotozoarios/efectos adversos , Antiprotozoarios/aislamiento & purificación , Peso Corporal , Pollos , Modelos Animales de Enfermedad , Heces/parasitología , India , Fitoquímicos/efectos adversos , Fitoquímicos/aislamiento & purificación , Extractos Vegetales/efectos adversos , Extractos Vegetales/aislamiento & purificación , Resultado del TratamientoRESUMEN
BACKGROUND AND OBJECTIVE: Presence of parasites in fishes has been reported to severely damage the status of antioxidants in them. Among helminth parasites, Pomphorhyncus an acanthocephalan parasite is commonly found in Schizothorax fish of Kashmir and is causing a considerable damage to fish health. The aim of this study was to investigate the antioxidant status in muscle, intestine and liver tissues of Schizothorax plagiostomus parasitized by Pomphorhyncus. MATERIALS AND METHODS: For this, 9 fish specimens collected from Nallah Sukhnag (Budgam) were found to be infected only with Pomphorhyncus, an acanthocephalan parasite. Fishes having the infection of Pomphorhynchus and the same number of uninfected ones were then analyzed for enzymatic antioxidants. Lipid peroxidation and protein content was also assayed for both infected and uninfected fishes. Statistically the whole data were represented as mean and standard deviation. RESULTS: RESULTS showed the reduction of glutathione-s-transferase (GST), superoxide dismutase (SOD), protein content while increase in lipid peroxidation in infected muscle, intestine and liver tissues as compared to respective organs of the uninfected fishes. CONCLUSION: This study concluded that parasitic infections induce oxidative stress.
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Despite presence of anticoccidial drugs and vaccines in the market, coccidiosis continues to result in substantial economic losses to the poultry industry. There is wide-spread resistance to already known anticoccidial drugs. It is an infectious disease of poultry and rigorous management is required during vaccination. In addition there is possibility of drug residues in meat and other byproducts of such treated animals and consequently makes more imperative to explore and understand the role of natural products in livestock parasite management. Therefore a study was designed to evaluate the anticoccidial activity of aqueous extract of Ganoderma applanatum in broiler chicken. In-vivo anticoccidial activity of aqueous extract of G. applanatum was measured in comparison to the reference drug amprolium on the basis of oocysts per gram of faeces, weight gain and feed conversion ratio. Oocyst output was measured with the help of Mc-Masters counting technique. The results of this study established the virulence of coccidian oocysts and the effectiveness of both amprolium and aqueous extract of G. applanatum against coccidian oocysts, confirmed by the fact that treatment with aqueous extract of G. applanatum resulted in a noticeable reduction in coccidian oocysts output, leading to improved weight gain and better feed conversion ratio. The study highlighted the potential of G. applanatum as a natural source of bioactive components for controlling a protozoan parasite, which can be isolated and tested in a bioassay-guided manner and harnessed in the form of anticoccidial drugs.
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Despite the advent of anticoccidial drugs and vaccines, coccidiosis continues to result in substantial economic losses to the poultry industry. Berberine, a natural alkaloid is well known in studies involving synergistic approaches, thereby reducing the dosage of principal drugs. Therefore, a study was designed to see whether a synergistic anticoccidial effect could be obtained between amprolium and berberine, in vivo using broiler chicken. Anticoccidial activity was measured in comparison to the reference drug amprolium on the basis of oocyst output reduction, mean weight gain and feed conversion ratio. Oocyst output was measured using Mc-Masters counting technique. Different combinations of berberine and amprolium were tested and out of which 1:1 ratio was the most effective for controlling these parasites. Oral gavaging of 100(50 + 50) mg/kg body weight of 1:1 ratio of amprolium and berberine caused the equivalent reduction in number of oocysts (38.85 ± 9.61) one day prior to that of standard drug amprolium (49.95 ± 16.65) as well as pure berberine (44.4 ± 9.61) used in the study. Weight gain of birds was also highest in the synergistic group (1547.43 ± 12.86) among all the infected groups. Besides feed conversion ratio in the synergistic group was also better (1.387 ± 0.026). The results of this study proved the effectiveness of both amprolium and berberine and revealed synergism between amprolium and berberine against coccidian oocysts, confirmed by significant reduction in the number of coccidian oocysts shed in the feces, leading to better weight gain and improved feed conversion ratio. The study deep-rooted the synergistic potential of berberine, a natural bioactive compound for controlling a protozoan parasite and the results of this study corroborate with its use for treatment of severe diarrhoea, amoebiasis and intestinal infections.
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Amprolio/administración & dosificación , Antiprotozoarios/administración & dosificación , Berberina/administración & dosificación , Coccidiosis/veterinaria , Extractos Vegetales/administración & dosificación , Enfermedades de las Aves de Corral/tratamiento farmacológico , Animales , Pollos , Coccidiosis/tratamiento farmacológico , Coccidiosis/parasitología , Sinergismo Farmacológico , Quimioterapia Combinada , Enfermedades de las Aves de Corral/parasitologíaRESUMEN
Vascular inflammation and associated ongoing inflammatory responses are considered as the critical culprits in the pathogenesis of acute atherothrombotic events such as acute coronary syndrome (ACS) and myocardial infarction (MI). ST segment elevation myocardial infarction (STEMI) is considered as one of the prominent clinical forms of ACS. Moreover, C-reactive protein (CRP) is an important acute phase prsotein, which may be estimated using high-sensitivity methods (hs-CRP), and its elevated level in body fluids reflects chronic inflammatory status. The circulating hs-CRP level has been proposed as a promising inflammatory marker of coronary artery disease (CAD). The present study investigated the correlation of hs-CRP level with clinical and angiographic features of STEMI, various other traditional risk factors, complications of myocardial infarction and angiographically significant CAD. Out of 190 patients with STEMI that were analyzed, the interval between symptom onset and reperfusion therapy (window period) varied from 0.5 to 24 h. The hs-CRP value was found to be higher in non-diabetic patients (0.61 mg/dl) compared with diabetic patients (0.87 mg/dl). Moreover, a significant correlation between hs-CRP and hs-troponin T was also recorded (P<0.001). However, there was no significant difference in the mean hs-CRP values in patients with or without mortality. It is considered that the present study will increase the understanding of atherosclerosis in general and may also have clinical applications in the targeting of therapy for this harmful disease.
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The information on the epidemiology and control of coccidian parasites of broilers in Kashmir valley is based on the reports available from other regions of the world. With this background, the present study was conducted to investigate the seasonal prevalence of the disease in the temperate agro-climatic conditions of Kashmir valley. A standard protocol for sampling was followed according to which five birds per 10,000 is sufficient to diagnose coccidiosis. Microscopic examination (under 10× and 40× objective lens) was used to reveal the presence of coccidial oocysts. Different species of genus Eimeria were identified on the basis of their predilection site, morphology and size. Coccidiosis was most prevalent in autumn 45.12 ± 2.55 (September 47.5 %, October 42.42 % and November 45.46 %) followed by summer 30.84 ± 6.86, spring 23.81 ± 2.81 and winter 20.29 ± 6.40. In summer, prevalence of disease was low but afterwards prevalence of disease rose up from August to October. In spite of high relative humidity in winter, disease showed low prevalence rate. Over all prevalence for the whole year was 29.87 %. Among species Eimeria tenella was the most dominant parasite showing highest prevalence of 18.13 %. Variation in incidence of coccidiosis with respect to seasons showed a strong correlation and data was found to be statistically significant with P < 0.05. The results obtained would be quite useful to devise appropriate and effective control strategies and prophylactic programs for coccidiosis in poultry unique to this climatic zone and other parts of the world with similar climatic and poultry production systems.
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During a random survey of parasitisation of poultry birds of Kashmir, conducted for 3 years more than 300 poultry birds were randomly collected from different parts of the Kashmir valley and were examined for the helminth parasites. The most dominant group of parasites which was parasitizing the poultry birds was found to be the Cestode, whose prevalence in the infected birds was 76.9 %. Nematodes were found to be the second dominant group of parasites after cestodes with the prevalence of 70 %. Among the different species of nematodes Heterakis gallinae were found infecting almost all the birds autopsied. The highest intensity of H. gallinae might be because of the fact that it requires cockroaches, beetles and grasshoppers as intermediate hosts in addition of having a direct life cycle. Nematodes which were recovered from the intestine and gizzard of the birds belong to the genera Capillaria, Acuaria, Amidostomum, Streptocara, Heterakis, and Ascaridia.
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AIM AND BACKGROUND: Heart-type fatty acid-binding proteins (H-FABP) which are detected within 2-3 h of acute myocardial infarction are involved in uptake of free fatty acids in the myocardium. Our aim in the present study is to compare window periods of H-FABP to high sensitivity troponin T (hs-Trop T) in acute ST elevation myocardial infarction (STEMI). METHODS: 160 STEMI diagnosed patient's serum samples are analyzed for hs-Trop T and H-FABP. Different window periods of chest pain onset (<3 h, 3-6 h and >6 h) are compared with complications, in-hospital mortality and statistically analyzed. RESULTS: From 160 patients, 53 (33%) cases are presented in <3 h, 75 (47%) in 3-6, and 32 (20%) after >6 h respectively. Accordingly sensitivity of hs-Trop T was 92%, 94% and 97% while H-FABP was 75%, 88% and 84%, respectively. Overall sensitivity was 94% and 82% respectively. Statistically significant difference between mean hs-Trop T values with respect to window period <3, 3-6 and >6 h was 0.21, 0.35 and 0.80 ng/ml respectively, p value < 0.0001. No significant difference in H-FABP values was observed. Hs-Trop T positively correlated with age (r = 0.153, P = 0.05), window period (r = 0.363, P < 0.0001), TIMI score (r = 0.208, P = 0.008), ejection fraction (r = 0.191, P = 0.008), serum H-FABP (r = 0.229, P = 0.004), and serum hs-CRP (r = 0.326, p < 0.001). There was a statistically significant difference of mean hs-Trop T values with or without in hospital mortality (0.35 vs. 0.85 ng/ml, respectively, p = 0.008). No significant correlation to age, TIMI score, ejection fraction and hs-CRP values for H-FABP was observed. CONCLUSION: It appears that hs-Trop T is a more sensitive marker than H-FABP in early hours of AMI and higher hs-Trop T predicts increase in-hospital mortality.
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Coccidiosis, caused by various Eimeria species, is a major parasitic disease in chicken. However the increasing resistance of these parasites to currently used anticoccidial drugs has stimulated the search for new methods of control. As part of this effort we investigated the root bark of Berberis lycium (barberry) as a potential source of compounds with anticoccidial activity. In the present study anticoccidial activity of different solvent extracts of the root bark of B. lycium and berberine was evaluated in vivo using broiler chicken. Results of the study demonstrated equipotent efficacy of pure berberine in comparison to that of standard drug amprolium on the basis of reduction in coccidian oocyst output, body weight gain of chicken and feed conversion ratio. Among the extracts crude methanolic extract showed highest anticoccidial activity tested at 300 mg/kg body weight which could be due to the presence of alcohol-soluble active ingredients in root bark of B. lycium. Toxicological studies revealed that B. lycium extracts as well as berberine were not lethal up to dosage of 2000 mg/kg body weight. LD(50) was not determined as mortalities were not recorded in any of the five groups of chicken. From the present study it can be concluded that root bark of B. lycium has the immense potential to contribute to the control of coccidian parasites of chicken. Our results corroborate the use of berberine for treatment of severe diarrhoea, amoebiasis and intestinal infections and could justify its use in folk medicine for treatment of haemorrhagic dysentery.
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Berberina/uso terapéutico , Berberis/química , Coccidiosis/veterinaria , Coccidiostáticos/aislamiento & purificación , Enfermedades de las Aves de Corral/prevención & control , Animales , Berberina/aislamiento & purificación , Berberina/farmacología , Pollos , Coccidiosis/prevención & control , Masculino , Recuento de Huevos de Parásitos , Fitoterapia , Corteza de la Planta/química , Extractos Vegetales/uso terapéutico , Raíces de Plantas/química , Aumento de Peso/efectos de los fármacosRESUMEN
An evaluation of Gastrothylax crumenifer crude antigen preparation viz., Somatic Antigen (SAg), Excretory Secretory Antigen (ESAg) and Egg Antigen (EAg) in serodiagnosis of disease was undertaken. Test sera samples were obtained from 30 Paramphistomiasis Positive and 30 Gastrothylax free sheep slaughtered at Hazratbal Kashmir. The referral antigenic preparation were evaluated against Paramphistomiasis positive sera, via., control negative sera, using double immunodiffusion test (DID), (IEP) Immunoelectrophoretic assay and ELISA. The performance of referral antigens, as assessed from percent sensitivity and specificity, revealed an increasing trend from DID (Double immunodiffusion-An immunological technique used in the detection, identification and quantification of antibodies and antigens) to IEP (immunoelectrophoresis-A general name for a number of biochemical methods for separation and characterization of proteins based on electrophoresis and reaction with antibodies), followed by ELISA, detecting higher number of sheep positive for paramphistomiasis. In ELISA the ESAg and SAg were evaluated as most reactive antigens with no significant difference and EAg was the least antigenic. In IEP, EAg had the higher sensitivity (60%) and analogous specificity of SAg and ESAg. The formation of the preceptin lines in the proximity to EAg containing wells (cathode end) in IEP was suggestive of higher molecular weight of G. crumenifer specific protein molecules with slower rate of migration. Purification and characterization of G. crumenifer and identification of specific antigenic molecules, particularly in EAg has been suggested for qualitative improvement of diagnostic value of the antigens in the tests used here in.