Simulation of limb frostbites in air medium.

The clinical course of frostbites of the limbs was studied in 3 animal species (swine, dog, rabbit) with frostbites of different intensity and duration. Animals of different species exhibited different sensitivity of their limb tissues to cold. The rabbit proved to be the most adequate animal for frostbite simulation. Frostbites of similar area and depth of lesions can be induced by cooling according to different protocols.

Effect of local application of superoxide dismutase on dielectric parameters of cooled skin in rats.

The effect of on Changes in dielectric parameters of the skin (modulus of complex dielectric permittivity |e| and dielectric loss tangent tgd) were studied on rats with local surface contact cooling followed by treatment with various cream formulations. Addition of antioxidant superoxide dismutase (SOD) to the cream significantly prevented the shifts in these parameters, which attested to less pronounced changes in the water balance in SOD-treated skin. Application of SOD during the early terms after cooling accelerated wound healing. Histological examination performed on posttraumatic day 60 revealed better integrity of the skin structures (hair follicle, sweat and sebaceous gland), which indicates ability of SOD to prevent and ameliorate the degree of cold-induced damage in the skin.

[Use of disc electrophoresis in polyacrylamide gel method for the separation of soluble proteins of vibrios]. / Primeneie metoda, diskovogo elektroforeza v poliakrilamidnom gele dlia razdeleniia rastvorimykh belkov vibrionov

The authors present the results of a comparative study of the protein spectra of 80 strains of various vibrios. Proteinograms were elaborated by the method of disc-electrophoresis in polyacrylamide gel for each strain and species. There were revealed 25 protein peaks with definite mobility coefficients. The number of peaks and their areas varied in different species. The qualitative and the quantitative differences between the vibrio species were established. Results of investigations demonstrated a possibliity of using the protein spectra for the differentiation of microorganisms.