RESUMEN
Efficient nutrient acquisition in the human gut is essential for microbial persistence. Although polysaccharides have been well-studied nutrients for the gut microbiome, other resources such as nucleic acids and nucleosides are less studied. We describe several ribose-utilization systems (RUSs) that are broadly represented in Bacteroidetes and appear to have diversified to access ribose from a variety of substrates. One Bacteroides thetaiotaomicron RUS variant is critical for competitive gut colonization in a diet-specific fashion. We used molecular genetics to probe the required functions of the system and the nature of the nutrient source(s) underlying this phenotype. Two RUS-encoded ribokinases were the only components required for this effect, presumably because they generate ribose-phosphate derivatives from products of an unlinked but essential nucleoside phosphorylase. Our results underscore the extensive mechanisms that gut symbionts have evolved to access nutrients and the potential for unexpected dependencies among systems that mediate colonization and persistence.
Asunto(s)
Bacteroides thetaiotaomicron , Pentosiltransferasa/genética , Fosfotransferasas (Aceptor de Grupo Alcohol)/genética , Ribosa/metabolismo , Animales , Bacteroides thetaiotaomicron/genética , Bacteroides thetaiotaomicron/metabolismo , Dieta , Microbioma Gastrointestinal/genética , Regulación Bacteriana de la Expresión Génica , Genes Bacterianos , Ratones , Pentosiltransferasa/metabolismo , Fosfotransferasas (Aceptor de Grupo Alcohol)/metabolismo , SimbiosisRESUMEN
SCOPE: Evidence suggests that dietary pattern may affect polyphenol absorption and/or metabolism. Further, obesity is associated with lower circulating nutrients, though the reason is unclear. We investigated the pharmacokinetic (PK) response of polyphenols in obese/overweight versus lean individuals before and after repeated dosing of grape polyphenols. METHODS AND RESULTS: A pilot study was conducted in which PK challenges were administered before and after 10 days of repeated dosing with polyphenols. Volunteers (6 lean, 6 overweight/obese) consumed resveratrol, grape seed extract, and grape juice (2125 mg total polyphenols) daily. On days 1 and 11, blood samples were collected for 6 h after the polyphenol dose and analyzed for deconjugated catechin, epicatechin, resveratrol, and quercetin. Area under the plasma polyphenol mass by time curves (AUCs) were greater for catechin, epicatechin, and quercetin on day 11 versus day 1 for low BMI individuals (p = 0.039) but not high BMI individuals. Further, AUCs were greater for epicatechin and resveratrol for low versus high BMI individuals (p = 0.041), with a similar trend for catechin (p = 0.065), on day 11 but not day 1. CONCLUSION: These results suggest that that obesity and repeated exposure may modify polyphenol absorption and/or metabolism in humans.
Asunto(s)
Fármacos Antiobesidad/administración & dosificación , Suplementos Dietéticos , Frutas/química , Obesidad/metabolismo , Sobrepeso/metabolismo , Polifenoles/administración & dosificación , Vitis/química , Anciano , Fármacos Antiobesidad/sangre , Fármacos Antiobesidad/metabolismo , Fármacos Antiobesidad/uso terapéutico , Área Bajo la Curva , Índice de Masa Corporal , Femenino , Jugos de Frutas y Vegetales , Humanos , Cinética , Masculino , Persona de Mediana Edad , Obesidad/sangre , Obesidad/dietoterapia , Sobrepeso/sangre , Sobrepeso/dietoterapia , Proyectos Piloto , Extractos Vegetales/administración & dosificación , Extractos Vegetales/metabolismo , Extractos Vegetales/uso terapéutico , Polifenoles/sangre , Polifenoles/metabolismo , Polifenoles/uso terapéutico , Resveratrol , Semillas/química , Estilbenos/administración & dosificación , Estilbenos/sangre , Estilbenos/metabolismo , Estilbenos/uso terapéuticoRESUMEN
Bacteriophage PhiV10 is a temperate phage, which specifically infects Escherichia coli O157:H7. The nucleotide sequence of the PhiV10 genome is 39 104 bp long and contains 55 predicted genes. PhiV10 is closely related to two previously sequenced phages, the Salmonella enterica serovar Anatum (Group E1) phage epsilon15 and a prophage from E. coli APEC O1. The attachment site of PhiV10, like those of its two closest relatives, overlaps the 3' end of guaA in the host chromosome. PhiV10 encodes an O-acetyltransferase, which modifies the O157 antigen. This modification is sufficient to block PhiV10 superinfection, indicating that the O157 antigen is most likely the PhiV10 receptor.