[Methotrexate-Induced Nephrotoxicity in Rats: Protective Effect of Mistletoe (Viscum album L.) Extract].

BACKGROUND: The protective effect of mistletoe extract (Helixor®, HLX) against methotrexate (MTX)-induced acute oxidative stress and nephrotoxicity in rats was evaluated by histological and biochemical methods as well as the comet assay. MATERIAL AND METHODS: 32 female Wistar albino rats were divided into 4 groups: control group, HLX group (5 mg/kg body weight (bw), days 1-10, intraperitoneally (i.p.)), MTX group (10 mg/kg bw, days 7, 8, and 9, i.p.), and MTX + HLX group (10 mg/kg bw, days 7, 8, and 9, i.p. + 5 mg/kg bw, days 1-10, i.p.). At the end of the experiment, the glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), nitric oxide (NO), and myeloperoxidase (MPO) levels were measured, and a histopathological analysis and comet assay were carried out. RESULTS: MTX induced renal oxidative stress and nephrotoxicity in the rats. Pretreatment with HLX significantly improved the renal GSH-Px and SOD activities in the MTX + HLX group compared to the MTX group. The decrease in the NO and MPO levels in the rat groups pretreated with HLX was not significant. The histochemical evaluation revealed that HLX provided significant improvement in the MTX-induced renal degenerative changes, including tubule distension, interstitial inflammation, perirenal inflammation, glomerular congestion, glomerular degeneration, and parenchymal hemorrhage, in the MTX + HLX group compared to the MTX-administered group. According to the comet assay, pretreatment with HLX lowered the MTX-induced DNA damage in endogenous lymphocytes, although not significantly. CONCLUSION: This study demonstrated that HLX administration markedly reduced the MTX-induced acute oxidative stress and nephrotoxicity in rats through its antioxidant and anti-inflammatory properties.

Pneumatization of crista galli in pre-adult and adult stages / Neumatización de la crista galli en etapas pre adultas y adultas

The sinonasal region in humans is one of the regions that commonly shows anatomical variations. These variations can be easily diagnosed by paranasal CT evaluation. One of these variations is Crista galli pneumatization. In recent years, there have been opinions supporting the hypothesis that pneumatization originates from the frontal sinus. In this study, we planned to evaluate whether the presence of Crista galli pneumatization varied in pre-adult and adult periods. In this retrospectively designed study, 218 coronal paranasal CT images collected between 2012 and 2013 were evaluated. Patients were divided into two groups according to age under or over 18 years. In the group under the age of 18 (97 cases), pneumatization was detected in 2.1 % of samples, while in the group over the age of 18 (121 cases), crista galli pneumatization was observed in 15.7 % of samples. According to these results, crista galli pneumatization was found to increase in adulthood. Considering that the frontal sinus is in a rudimentary state at birth, it is radiographically detected first at 6 years of age, and reaches its main size in puberty, this increase in pneumatization runs parallel to the development of the frontal sinus. Consequently, this supports the opinion that crista galli pneumatization originates from the frontal sinus.

La región nasosinusal en los seres humanos con frecuencia muestran variaciones anatómicas. Estas variaciones se pueden diagnosticar fácilmente mediante la evaluación por tomografía computadorizada (TC) de los senos paranasales. Una de estas variaciones es la neumatización de la Crista galli. En los últimos años, se ha apoyado la hipótesis de que ésta neumatización se origina en el seno frontal. En este estudio fue evaluada la presencia de neumatización de la Crista galli y su posible variación en los períodos pre-adultos y adultos. Se realizó un estudio retrospectivo donde se evaluaron 218 imágenes de TC coronal de senos paranasales, recogidas entre 2012 y 2013. Los pacientes fueron divididos en dos grupos según la edad, menores o mayores de 18 años. En el grupo de menores de 18 años (97 casos), se detectó neumatización en el 2,1 % de las muestras, mientras que en el grupo de mayores de 18 (121 casos), se observó neumatización de la Crista galli en el 15,7 % de las muestras. De acuerdo con estos resultados, la neumatización de la Crista galli aumenta en la edad adulta. Teniendo en cuenta que el seno frontal se encuentra en un estado rudimentario en el nacimiento, se detecta radiográficamente a los 6 años de edad alcanzando su tamaño principal en la pubertad; este aumento de la neumatización es paralelo al desarrollo del seno frontal. En consecuencia, esto apoya la opinión de que la neumatización de la Crista galli se origina en el seno frontal.

The Causes of Persistent and Recurrent Nasal Obstruction After Primary Septoplasty.

OBJECTIVES: Septoplasty is the most frequently performed surgical procedure in patients with nasal obstruction. However, nasal obstruction may persist or recur after septoplasty in some patients. In this study, the authors aimed to determine the causes of nasal obstruction after septoplasty. METHODS: Fifty consecutive patients who admitted to our clinic with the complaints of persistent or recurrent nasal obstruction after primary septoplasty and had revision surgery between 2011 and 2015 were included in this study. Demographic data and physical examination findings of the patients were recorded. RESULTS: There were 33 men (66%) and 17 women (34%) with a mean age of 37.3 ±â€Š10.5 (range, 21-57) years. Deviation of perpendicular plate of ethmoid bone (44%), inferior turbinate hypertrophy (36%), concha bullosa (26%), caudal septal deviation-nostril asymmetry (20%), and alar collapse (6%) were the pathologies that were unaddressed during primary surgery. The iatrogenic causes of nasal obstruction after septoplasty were collumellar retraction-nasal tip ptosis (46%), nasal synechiae (20%), nasal septal perforation (10%), and saddle-nose/flat nose deformity (10%). CONCLUSIONS: Iatrogenic deformities due to surgery and pathologies ignored during primary surgery may cause persistent/recurrent nasal obstruction after primary septoplasty. A detailed physical examination and objective tests showing the site of nasal obstruction, an extensive surgical plan that covers all diagnosed pathologies, and a careful postoperative care must be undertaken to prevent undesired postoperative results.

Evaluation of the vertebral artery by 3D digital subtraction angiography / Evaluación de la arteria vertebral mediante angiografía por sustracción digital 3D

The objective of the present study is to determine origin, entry level to the transverse foramen and diameter according to sex and side through 3D angiographic images of seventy-nine vertebral arteries obtained using DSA imaging method. During radiological evaluation, axial, coronal and sagittal images of the artery were used. Data of our study was uploaded to SPSS 14.0 program and significance test and Mann Whitney-U test of the difference between two means were used to evaluate the data. While 76 of the vertebral arteries (96.2%) were originating from the subclavian artery, 3 of them (3.8%) were directly originating from aortic arch. In 67 of 76 the vertebral arteries originating from the subclavian artery were entering through C6, 6 through C7, 2 through C5 and one through C4 transverse foramen. It was seen that one of 3 the vertebral artery originating from aortic arch was entering through C6, one through C7 and the other through C4 transverse foramen. While the mean diameter of the vertebral artery was 3.88±0.71 mm at the right side (3.99 mm in men, 3.66 mm in women), the mean diameter at the left side was 4.15±1.05 mm (4.23 mm in men, 4.06 mm in women).

El objetivo fue determinar el origen, nivel de entrada y diámetro del foramen transverso, en función del sexo y lado sobre 79 arterias vertebrales a través de imágenes angiográficas 3D utilizando el método de formación de imágenes DSA. Durante la evaluación radiológica, se utilizaron imágenes axiales, coronales y sagitales de la arteria. Los datos se procesaron en el programa SPSS 14.0 con las pruebas U de Mann Whitney y de significancia para evaluar la diferencia entre los datos. Mientras que 76 de las arterias vertebrales (96,2%) se originaron de la arteria subclavia, 3 de ellas (3,8%) lo hicieron directamente desde el arco aórtico. En 67 de 76 arterias vertebrales, se observó el origin en la arteria subclavia a través de C6; 6 a través de C7, 2 a través de C5 y una a través del foramen transverso en C4. El diámetro medio de la arteria vertebral fue 3,88±0,71 mm en el lado derecho (3,99 mm en hombres y 3,66 mm en mujeres) y en el lado izquierdo fue 4,15±1,05 mm (4,23 mm en hombres y 4,06 mm en hombres).

Frontal lobe morphometry with MRI in a normal age group of 6-17 year-olds.

BACKGROUND: Morphometric data of the frontal lobe are important for surgical planning of lesions in the frontal lobe and its surroundings. Magnetic resonance imaging (MRI) techniques provide suitable data for this purpose. OBJECTIVES: In our study, the morphometric data of mid-sagittal MRI of the frontal lobe in certain age and gender groups of children have been presented. PATIENTS AND METHODS: In a normal age group of 6-17-year-old participants, the length of the line passing through predetermined different points, including the frontal pole (FP), commissura anterior (AC), commissura posterior (PC), the outermost point of corpus callosum genu (AGCC), the innermost point of corpus callosum genu (IGCC), tuberculum sella (TS), AGCC and IGCC points parallel to AC-PC line and the point such line crosses at the frontal lobe surface (FCS) were measured in three age groups (6-9, 10-13 and 14-17 years) for each gender. RESULTS: The frontal lobe morphometric data were higher in males than females. Frontal lobe measurements peak at the age group of 10-13 in the male and at the age group of 6-13 in the female. In boys, the length of FP-AC increases 4.1% in the 10-13 age group compared with the 6-9-year-old group, while this increase is 2.3% in girls. CONCLUSION: Differences in age and gender groups were determined. While the length of AGCC-IGCC increases 10.4% in adults, in children aged 6-17, the length of AC-PC is 11.5% greater than adults. These data will contribute to the preliminary assessment for developing a surgical plan in fine interventions in the frontal lobe and its surroundings in children.